TW202340454A - 將誘導性多能幹細胞分化為視網膜色素上皮細胞之方法、視網膜色素上皮細胞以及使用該視網膜色素上皮細胞之方法 - Google Patents

將誘導性多能幹細胞分化為視網膜色素上皮細胞之方法、視網膜色素上皮細胞以及使用該視網膜色素上皮細胞之方法 Download PDF

Info

Publication number
TW202340454A
TW202340454A TW112103136A TW112103136A TW202340454A TW 202340454 A TW202340454 A TW 202340454A TW 112103136 A TW112103136 A TW 112103136A TW 112103136 A TW112103136 A TW 112103136A TW 202340454 A TW202340454 A TW 202340454A
Authority
TW
Taiwan
Prior art keywords
cells
rpe
medium
days
culture
Prior art date
Application number
TW112103136A
Other languages
English (en)
Chinese (zh)
Inventor
心怡 蘇
雷加 卡卡德
馬尤里 巴爾加瓦
楊賓霞
沃爾特 亨齊克
劉增平
王昊飛
巴夫哈沙德 帕里克
清烽 陳
憲忠 賴
兆 蔡
嘉隆 林
全胜 潘
Original Assignee
新加坡科技研究局
新加坡商細胞研究私人有限公司
新加坡商新加坡保健服務私人有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 新加坡科技研究局, 新加坡商細胞研究私人有限公司, 新加坡商新加坡保健服務私人有限公司 filed Critical 新加坡科技研究局
Publication of TW202340454A publication Critical patent/TW202340454A/zh

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C12N2506/025—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells from extra-embryonic cells, e.g. trophoblast, placenta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Ophthalmology & Optometry (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
TW112103136A 2022-01-27 2023-01-30 將誘導性多能幹細胞分化為視網膜色素上皮細胞之方法、視網膜色素上皮細胞以及使用該視網膜色素上皮細胞之方法 TW202340454A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202263303849P 2022-01-27 2022-01-27
US63/303,849 2022-01-27

Publications (1)

Publication Number Publication Date
TW202340454A true TW202340454A (zh) 2023-10-16

Family

ID=87472757

Family Applications (1)

Application Number Title Priority Date Filing Date
TW112103136A TW202340454A (zh) 2022-01-27 2023-01-30 將誘導性多能幹細胞分化為視網膜色素上皮細胞之方法、視網膜色素上皮細胞以及使用該視網膜色素上皮細胞之方法

Country Status (8)

Country Link
US (1) US20250197803A1 (https=)
EP (1) EP4469561A4 (https=)
JP (1) JP2025507265A (https=)
CN (1) CN119497749A (https=)
AU (1) AU2023212665A1 (https=)
CA (1) CA3249352A1 (https=)
TW (1) TW202340454A (https=)
WO (1) WO2023146477A2 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2022191216A1 (https=) * 2021-03-09 2022-09-15

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2951290B1 (en) * 2013-02-01 2017-11-29 The United States of America, as represented by The Secretary, Department of Health and Human Services Method for generating retinal pigment epithelium (rpe) cells from induced pluripotent stem cells (ipscs)
CN108531443A (zh) * 2018-04-04 2018-09-14 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 小分子诱导多能性干细胞分化为视网膜色素上皮细胞的方法
CN110205283B (zh) * 2018-04-18 2021-04-23 浙江大学 一种诱导人羊膜上皮细胞向视网膜色素上皮细胞分化的方法及其应用

Also Published As

Publication number Publication date
AU2023212665A1 (en) 2024-08-15
EP4469561A2 (en) 2024-12-04
JP2025507265A (ja) 2025-03-18
WO2023146477A2 (en) 2023-08-03
CA3249352A1 (en) 2023-08-03
US20250197803A1 (en) 2025-06-19
CN119497749A (zh) 2025-02-21
WO2023146477A3 (en) 2023-10-19
EP4469561A4 (en) 2026-02-25

Similar Documents

Publication Publication Date Title
JP7656949B2 (ja) ミエリン障害の治療のための誘導多能性細胞由来オリゴデンドロサイト前駆細胞
JP7407865B2 (ja) 細胞の再プログラミングのための方法とその用途
AU2025223802A1 (en) Cells differentiated from immunoengineered pluripotent cells
Tucker et al. Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation
US9926532B2 (en) Method of generating induced pluripotent stem cells and differentiated cells
CN100554411C (zh) 来自角膜缘的未分化干细胞组织系统
JP7055638B2 (ja) 幹細胞からの筋肉系列細胞の生成
JP6603529B2 (ja) 再プログラム化多能性細胞の作製
JP7829191B2 (ja) 人工多能性幹細胞を作製する方法、人工多能性幹細胞、および人工多能性幹細胞を使用する方法
US20150376574A1 (en) Pancreatic insulin-producing beta-cell lines derived from human pluripotent stem cells
KR20110022713A (ko) 인간 만능줄기세포로부터 인간 피부 대체물을 제조하는 방법
EP2481795A1 (en) Method of generating induced pluripotent stem cells and differentiated cells
JP2016516394A (ja) ヒト線維芽細胞において特徴的な幹細胞であるＭｕｓｅ細胞から容易に分化した機能性メラノサイトの使用
JP4576545B2 (ja) 羊膜由来因子による胚性幹細胞の培養方法
TW202340454A (zh) 將誘導性多能幹細胞分化為視網膜色素上皮細胞之方法、視網膜色素上皮細胞以及使用該視網膜色素上皮細胞之方法
US20160304840A1 (en) Method for Producing Induced Pluripotent Stem Cells
TWI769410B (zh) 新穎誘導性多能幹細胞(ipscs)及其應用
Huang et al. Induced pluripotent stem cell technologies for tissue engineering
Martínez Sarrà Characterization of Dental Pulp Pluripotent-like Stem Cells (DPPSC) and their mesodermal differentiation potential.
Henning Identification and characterisation of a novel, multi-potent, skeletal muscle-derived stem cell with broad developmental plasticity
Muggeo A STEP-BY-STEP PROCESS TO GENERATE FUNCTIONAL OSTEOCLASTS FROM SITE SPECIFIC GENE-CORRECTED INDUCED PLURIPOTENT STEM CELLS: AN AUTOLOGOUS CELL THERAPY APPROACH TO TREAT AUTOSOMAL RECESSIVE OSTEOPETROSIS.
Class et al. Patent application title: PANCREATIC INSULIN-PRODUCING BETA-CELL LINES DERIVED FROM HUMAN PLURIPOTENT STEM CELLS Inventors: Dodanim Talavera-Adame (Canyon Country, CA, US) Donald C. Dafoe (Santa Monica, CA, US) Assignees: CEDARS-SINAI MEDICAL CENTER