CA3249352A1 - METHOD FOR DIFFERENTIATING AN INDUCED MULTIPOTENTED STEM CELL INTO A RETINAL PIGMENTED EPITHELIAL CELL, RETINAL PIGMENTED EPITHELIAL CELL AND METHODS FOR USING THE RETINAL PIGMENTED EPITHELIAL CELL - Google Patents

METHOD FOR DIFFERENTIATING AN INDUCED MULTIPOTENTED STEM CELL INTO A RETINAL PIGMENTED EPITHELIAL CELL, RETINAL PIGMENTED EPITHELIAL CELL AND METHODS FOR USING THE RETINAL PIGMENTED EPITHELIAL CELL

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Publication number
CA3249352A1
CA3249352A1 CA3249352A CA3249352A CA3249352A1 CA 3249352 A1 CA3249352 A1 CA 3249352A1 CA 3249352 A CA3249352 A CA 3249352A CA 3249352 A CA3249352 A CA 3249352A CA 3249352 A1 CA3249352 A1 CA 3249352A1
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Canada
Prior art keywords
cell
rpe
medium
days
ips
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3249352A
Other languages
English (en)
French (fr)
Inventor
Xinyi SU
Regha KAKKAD
Mayuri Bhargava
Binxia YANG
Walter Hunziker
Zengping LIU
Haofei WANG
Bhav Harshad PARIKH
Qingfeng Chen
Fritz Shien Choong LAI
Chou (deceased) (Décédé(e)/Deceased) CHAI
Kah Leong Lim
Toan Thang Phan
Original Assignee
Singapore Health Services Pte Ltd
CellResearch Corp Pte Ltd
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Filing date
Publication date
Application filed by Singapore Health Services Pte Ltd, CellResearch Corp Pte Ltd filed Critical Singapore Health Services Pte Ltd
Publication of CA3249352A1 publication Critical patent/CA3249352A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C12N2506/025—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells from extra-embryonic cells, e.g. trophoblast, placenta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Ophthalmology & Optometry (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA3249352A 2022-01-27 2023-01-27 METHOD FOR DIFFERENTIATING AN INDUCED MULTIPOTENTED STEM CELL INTO A RETINAL PIGMENTED EPITHELIAL CELL, RETINAL PIGMENTED EPITHELIAL CELL AND METHODS FOR USING THE RETINAL PIGMENTED EPITHELIAL CELL Pending CA3249352A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202263303849P 2022-01-27 2022-01-27
US63/303,849 2022-01-27
PCT/SG2023/050051 WO2023146477A2 (en) 2022-01-27 2023-01-27 A method of differentiating an induced pluripotent stem cell into a retinal pigment epithelial cell, a retinal pigment epithelial cell and methods of using the retinal pigment epithelial cell

Publications (1)

Publication Number Publication Date
CA3249352A1 true CA3249352A1 (en) 2023-08-03

Family

ID=87472757

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3249352A Pending CA3249352A1 (en) 2022-01-27 2023-01-27 METHOD FOR DIFFERENTIATING AN INDUCED MULTIPOTENTED STEM CELL INTO A RETINAL PIGMENTED EPITHELIAL CELL, RETINAL PIGMENTED EPITHELIAL CELL AND METHODS FOR USING THE RETINAL PIGMENTED EPITHELIAL CELL

Country Status (8)

Country Link
US (1) US20250197803A1 (https=)
EP (1) EP4469561A4 (https=)
JP (1) JP2025507265A (https=)
CN (1) CN119497749A (https=)
AU (1) AU2023212665A1 (https=)
CA (1) CA3249352A1 (https=)
TW (1) TW202340454A (https=)
WO (1) WO2023146477A2 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2022191216A1 (https=) * 2021-03-09 2022-09-15

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2951290B1 (en) * 2013-02-01 2017-11-29 The United States of America, as represented by The Secretary, Department of Health and Human Services Method for generating retinal pigment epithelium (rpe) cells from induced pluripotent stem cells (ipscs)
CN108531443A (zh) * 2018-04-04 2018-09-14 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 小分子诱导多能性干细胞分化为视网膜色素上皮细胞的方法
CN110205283B (zh) * 2018-04-18 2021-04-23 浙江大学 一种诱导人羊膜上皮细胞向视网膜色素上皮细胞分化的方法及其应用

Also Published As

Publication number Publication date
AU2023212665A1 (en) 2024-08-15
EP4469561A2 (en) 2024-12-04
JP2025507265A (ja) 2025-03-18
WO2023146477A2 (en) 2023-08-03
US20250197803A1 (en) 2025-06-19
CN119497749A (zh) 2025-02-21
WO2023146477A3 (en) 2023-10-19
TW202340454A (zh) 2023-10-16
EP4469561A4 (en) 2026-02-25

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