TW202333837A - 萃取生物分子之裝置及方法 - Google Patents
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Abstract
一種萃取生物分子之裝置,包括一膜管及一容器,其中該膜管包括一膜管上部及一膜管下部,該膜管上部具有一上部開口,該膜管下部容納通透性膜,並具有一下部開口,該容器包括一容器上部及一容器下部,該容器上部具有一開口,允許該膜管被推入該容器內並在該膜管與該容器之間形成液密,該容器下部具有一封閉端,在使用該裝置時,將適量液體加入該容器中,再將該膜管推入該容器中,使該容器內之液體受該膜管之擠壓而流進膜管下部開口,進而通過該通透性膜而容納於該膜管內之膜上之空間內,達成萃取生物分子之目的。
Description
本發明係關於一種萃取生物分子之裝置及方法。
膜管常被使用以萃取生物檢體中之特定蛋白質、核酸等生物分子。膜管為一種具有通透性膜之管狀容器,其上部具有一開口,下部則容納該通透性膜並具有一下部開口。傳統操作方式是將液體加入膜管,以適當裝置和作用力如離心機產生的離心力或真空泵產生的大氣壓力差等方法進行操作。實施萃取的方法通常包含三個基本步驟:
1. 聚留:加入液態檢體於膜管中,施以適當的作用力推動液態檢體通過膜管下部開口,在過程中液態檢體內之特定的生物分子會被聚留在膜上。
2. 清洗:加入清洗液於膜管中並施以作用力,讓膜管內殘留的雜質通過膜而排除到膜管外。
3. 回收:加入溶解液於膜管中以將膜上的目標生物分子回溶,並施以作用力將其推出管外而進入另一個新的容器,完成回收目標生物分子的目的。
上述傳統的操作方法需利用離心機或真空泵等裝置產生作用力推動膜管內液體通過膜往下部開口流出,以進行聚留、清洗、回收等步驟,其中使用離心機裝置之操作較繁複,不適合大量檢體的操作;如果使用真空泵,則回收步驟需使用不同於聚留及清洗步驟使用的專用真空泵裝置,以確保回收步驟是在無污染的狀況下進行,因此須額外使用一套真空泵裝置,對於自動化設備之設計及自動化之實施會增加一些困難。
本發明之目的在於簡化操作的方法及裝置,以利萃取生物分子之大量化檢體處理及簡化自動化之實施。在本發明中,膜管被推入容器時產生的擠壓力讓容器內的液體逆向由膜管下部開口進入膜管內,其操作方法是加入適量液體於一容器中,當膜管被推入該容器並在該膜管與該容器之間形成液密時,該容器內的液體受膜管的擠壓,液體會從膜管下部的開口進入管內並通過膜而流入膜管內。相對於傳統方法,本發明僅需要膜管和容器等之簡單裝置,不需要離心機或真空泵等裝置;液體的流動方向由膜管下部開口進入膜管內,不是傳統方法中由膜管內向膜管下部開口流出,兩者在膜管內液體流動方向完全相反;關於產生擠壓力裝置的設計也是相對容易,如同注射器裝置,活塞桿被推入針筒而與針筒之間形成液密的狀態並產生擠壓力將液體注射出,在本發明中,膜管相當於活塞桿而容器相當於針筒,惟針筒前端有一孔洞而本發明之容器則是封閉的,針筒前端的孔洞改設在膜管下部的開口,注射器裝置和本發明的裝置雖然不同,但操作方式則近似。本發明使用膜管及容器之簡單擠壓裝置即可取代傳統的離心機和真空泵等裝置,尤其是應用在回收的步驟的簡化更為明顯,可免除離心機複雜的步驟或額外的專用真空泵裝置,溶解液可以留在原膜管內直接取用,無需額外的容器以接取該溶解液,故可簡化操作裝置及步驟,使膜管萃取生物分子的方法更容易在自動化的機台上實施。
本發明之萃取生物分子之裝置,包括一膜管10及一容器20。圖1及圖2分別顯示膜管10及容器20之外觀。
膜管10係業界普遍使用之一種器材,其在外觀上大致呈一圓管狀,可分為一上部11及一下部12,該上部11具有一上部開口13,該膜管下部12容納通透性膜14,並具有一下部開口15。
圖2顯示容器20之外觀。該容器20大致上亦為圓管狀,可分為一上部21及一下部22。該容器上部21具有一開口23,該容器下部22具有一封閉端24。
使用該裝置時,加入液體於該容器20中(見圖3)。當該膜管10被推入該容器20中時(見圖4),該容器20內之液體受該膜管10之擠壓而從該膜管下部開口15進入,並通過該通透性膜14而流入該膜管10內。
膜管10和容器20之設計必需配合,使得當膜管10被推入容器20時,膜管10與容器20間之界面必需形成液密,使得容器20內之液體不會從該界面洩漏,而能從膜管下部之開口15通過通透膜14而進入膜管10。
利用上述膜管10及容器20萃取生物分子比傳統方法更適合自動化。在萃取生物分子步驟的回收程序中,將溶解液加入容器20內,再將含有通透性膜14之膜管10由容器20上部開口23推入容器20中,由於該容器20與該膜管10之間係液密,該容器20內的液體受膜管10的擠壓而從膜管下部開口15進入,並通過通透性膜14而流入膜管10內。
本發明與傳統方法在回收過程有極大之差異。在傳統方法中,係將溶解液加在膜管中,溶解液可將膜上的生物分子回溶,然後再施以作用力(如離心力或氣壓力)將溶解液推出而進入另一個新的容器,完成回收目標生物分子的目的。在本發明的方法中,溶解液係加在容器20內。將膜管10由容器20上部開口推入容器20中時,容器20內之溶解液受膜管10之擠壓而從膜管下部開口15進入,並通過通透性膜14而流入膜管10內。通過通透性膜14之溶解液(即回收液)係留在膜管10內,因此,無需再使用離心機或真空泵裝置或另一支新的容器,在自動化的實施及操作上可以更簡化,使膜管萃取生物分子的方法更容易在自動化的機台上實施。
以上所述之本發明內容,僅是實施本發明之一可能方式,針對上述實施例做出修改、替換及組合而為本發明領域中熟悉此項技術者可輕易完成者,應視為在本發明概念之範圍內。
10:膜管
11:膜管上部
12:膜管下部
13:(膜管)上部開口
14:通透性膜
15:(膜管)下部開口
20:容器
21:容器上部
22:容器下部
23:(容器)上部開口
24:(容器)下部封閉端
圖1顯示膜管之外觀;
圖2顯示容器之外觀;
圖3顯示容器裝有液體之狀態;及
圖4顯示膜管推入容器之狀態。
10:膜管
11:膜管上部
12:膜管下部
13:(膜管)上部開口
14:通透性膜
15:(膜管)下部開口
20:容器
23:(容器)上部開口
24:(容器)下部封閉端
Claims (2)
- 一種萃取生物分子之裝置,包括: 一膜管,包括一膜管上部及一膜管下部,該膜管上部具有一上部開口,該膜管下部容納通透性膜,並具有一下部開口; 一容器,包括一容器上部及一容器下部,該容器上部具有一開口,使該膜管被推入該容器上部之該開口,在該膜管與該容器之間形成液密,該容器下部具有一封閉端。
- 一種利用膜管萃取生物分子之方法,包括: 將適量液體加入如請求項1所述之容器中; 將如請求項1所述之膜管推入該容器中,使該容器內之液體受該膜管之擠壓而從該膜管下部開口進入,並通過該通透性膜。
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TW111105538A TWI802273B (zh) | 2022-02-16 | 2022-02-16 | 萃取生物分子之裝置及方法 |
US18/078,094 US20230256395A1 (en) | 2022-02-16 | 2022-12-09 | Device and method for extracting biomolecules |
DE102022132784.1A DE102022132784A1 (de) | 2022-02-16 | 2022-12-09 | Vorrichtung und verfahren zur extraktion von biomolekülen |
CN202211585494.5A CN116606722A (zh) | 2022-02-16 | 2022-12-09 | 萃取生物分子的装置和方法 |
KR1020220171115A KR20230123425A (ko) | 2022-02-16 | 2022-12-09 | 생체 분자를 추출하기 위한 디바이스 및 방법 |
JP2022196832A JP2023119556A (ja) | 2022-02-16 | 2022-12-09 | 生体分子を抽出するためのデバイスおよび方法 |
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- 2022-12-09 US US18/078,094 patent/US20230256395A1/en active Pending
- 2022-12-09 DE DE102022132784.1A patent/DE102022132784A1/de active Pending
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Publication number | Publication date |
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KR20230123425A (ko) | 2023-08-23 |
DE102022132784A1 (de) | 2023-08-17 |
TWI802273B (zh) | 2023-05-11 |
US20230256395A1 (en) | 2023-08-17 |
CN116606722A (zh) | 2023-08-18 |
JP2023119556A (ja) | 2023-08-28 |
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