TW202208062A - Pipette cover and assembling device thereof that comprises a closure part for sealing a pipette container and a fitting notch for detachable connection with a drive device - Google Patents

Abstract

Disclosed are a pipette cover and an assembling device thereof, which are mainly such that a pipette cover is used to seal a pipette container for moving between different pipette racks for automatized preparation, analysis, and inspection of biological samples, in order to make an automatic handling process more efficient. The pipette cover includes a closure part for sealing the pipette container and a fitting notch for detachable connection with a drive device. The drive device is provided with a positioning part at a bottom and a rod movable up and down along the drive device and the positioning part for fit into the fitting notch of the pipette cover so as to make the pipette cover forming the detachable connection.

Description

Microtube top cover and its assembly device

The present invention relates to a microtube cap and its assembling device, in particular to a microtube cap that is used to seal the microtube cap to a microtube container and move it to different microtube racks for automatic biological sample preparation, analysis and inspection, and automatic processing. Process more efficient assembly device.

Press; In recent years, in order to improve the consistency of inspection quality and improve the limitations of traditional manual inspection methods, in the field of molecular medicine inspection, automated instruments and equipment have been successfully developed and implemented. However, the common automated biological sample processing and testing equipment on the market, especially the systems used in the field of molecular testing, usually use micro-well plates (such as 24, 48, 96, 384 or 1536-well plates) or multi-connection consumables. Discharge microtubes, which have limitations in the application of automated inspections. The conventional sealed micro-porous disk system is covered by film hot pressing, but this method is not easy to seal, and it is difficult to make every hole on the porous disk can be completely sealed. There is a heat vapor dissipation after heating, which leads to a short reaction sample volume, resulting in experimental results. The risk of misjudgment of the reading data, or the leakage of liquid between different holes, the potential risk of cross-contamination. In addition, the process of detecting biological samples in the prior art is prone to unnecessary waste of resources. For example, because the micro-well plate is a one-time-use consumable, when the number of samples to be tested is low, even if there are many unused holes in it, Once used, the micro-porous disc must be discarded directly, which indirectly results in waste of resources.

In view of this, the applicant, after continuous research and experimentation, came to design a microtube top cover and an assembly device thereof, which improved the deficiencies of the prior art and the defects of automatic detection instruments, thereby improving the detection efficiency.

The main purpose of the present invention is to provide a microtube cap and its assembly device, which are used to seal the microtube cap to the microtube container and move it to different microtube racks for automated biological sample preparation, analysis and inspection, avoiding airtightness Incomplete leakage risk, making the automated process more efficient.

The aforesaid microtube top cover and its assembling device use a driving device to form a detachable connection to the microtube top cover, and then cover the microtube top cover on the microtube container, and move the driving device mechanically according to different requirements. To extract and/or transfer the microtube container connected to the microtube top cover for automated biological sample preparation analysis and testing, thereby avoiding the risk of leakage due to incomplete sealing.

The aforementioned microtube top cover and its assembling device, wherein the microtube top cover is provided with a cover portion for sealing the microtube container, and an embedded groove for detachable connection with a driving device; the driving device The bottom edge is provided with a positioning portion, and a rod body that can move up and down along the driving device and the positioning portion to be embedded in the embedding groove of the upper cover of the microtube, so that the upper cover of the microtube can be connected in a detachable manner.

In the above-mentioned microtube top cover and its assembling device, the driving device is provided with a slide rail, and a rod body is provided on the slide rail, so that the rod body can move up and down along the slide rail.

In the above-mentioned assembly structure of the upper cover of the micro-tube, wherein the sliding rail of the driving device is provided with a limit groove of appropriate length, the rod system is provided with a horizontal protrusion at an appropriate position, and is located at the wall of the limit groove, so that the rod When the body moves up and down along the slide rail, the position limit of the rod body can be formed by the limit groove.

In the above-mentioned microtube top cover and its assembling device, the positioning portion provided on the driving device is in the shape of a sleeve, and the upper edge thereof is provided with a locking portion, which is locked into the sliding rail and fixed to the bottom end of the driving device by the locking portion. .

The aforementioned cap for microtubes and the assembling device thereof, wherein the cap for microtubes at least comprises a cap portion, and a wall portion extending outward from the top edge of the cap portion, and the inner edge of the cap constitutes an embedded groove, The rod body of the driving device extends into the embedded groove and is tightly combined with the upper cover of the micro-tube.

The aforementioned microtube upper cover and its assembling device, wherein the inner edge of the microtube upper cover wall extends horizontally and vertically outwards to form a clamping groove, so that the opening of the microtube container can be embedded in the clamping groove and tightly combined Put a cap on the microtube, and then seal the biological sample.

Please refer to FIG. 1 and FIG. 2 at the same time, which are an exploded perspective view and a cross-sectional view of the present invention. As shown in the figure, the present invention includes a driving device 1 , a microtube upper cover 2 and a microtube container 3 . The driving device 1 is provided with a sliding rail 11 , and the sliding rail 11 is provided with a rod body 12 which is driven by the driving device 1 and can move up and down along the sliding rail 11 . In this embodiment, the driving device 1 can be moved mechanically (the term "mechanical movement" in this specification refers to the mechanized arm module mounted on the conventional automated biological sample processing and testing equipment, the mechanized The arm usually moves in a two-dimensional or three-dimensional direction in a linear manner); the slide rail 11 is provided with a limit groove 111 of appropriate length, and the rod body 12 is provided with a horizontal protrusion at an appropriate position, and is located between the limit groove 111 The wall portion 121 is used to limit the position of the rod body 12 by the upper and lower ends of the limiting groove 111 when the rod body 12 moves up and down.

The driving device 1 is provided with a positioning portion 13 on the bottom edge of the slide rail 11 . In this embodiment, the positioning portion 13 is in the shape of a sleeve, the upper edge of which is provided with a screwing portion 131, and the locking portion 131 is locked into the sliding rail 11 and fixed to the bottom end of the driving device 1, so that the aforementioned rod The bottom end of 12 passes through the positioning portion 13 .

The microtube upper cover 2 at least includes a cover portion 21 and a wall portion 22 extending horizontally and vertically outward from the top edge of the cover portion 21. The inner edge of the upper cover 2 forms an embedded groove 23. The diameter of the setting groove 23 is equivalent to the diameter of the aforementioned positioning portion 13; the inner edge of the wall portion 22 forms a clamping groove 24, and the diameter of the clamping groove 24 is equivalent to the diameter of the opening of the aforementioned microtube container 3; wherein, the aforementioned cover The joint portion 21 is used to be inserted into the hole of the micro-tube container 3 or the micro-porous plate, so as to seal the biological sample to be tested in the hole of the micro-container 3 or the micro-porous plate. The lower end of the rod body 12 of the aforesaid driving device 1 can extend into the embedded groove 23 and be tightly combined with the upper cover 2 of the microtube. In this embodiment, the capping portion 21 is transparent, and various light sources with a wavelength of 300-800 nm can be introduced to excite the fluorescent substances contained in the biological sample in the tube, and at the same time, the fluorescent light can be exported through the capping portion 21, so as to excite the fluorescent substance contained in the biological sample in the tube. For optical detection.

The diameter of the opening of the microtube container 3 is equivalent to the diameter of the clamping groove 24 of the upper cover 2 of the microtube, which is used to accommodate blood, body fluids, animal tissues, plant tissues, viruses, free nucleic acids, and eukaryotic or A biological sample of prokaryotic cells awaiting detection.

With the aforementioned components, the mechanical movement driving device 1 can be used to extract and or move the microtube container 3 connected to the microtube upper cover 2 according to different requirements for automatic biological sample preparation, analysis and testing, avoiding the leakage risk of incomplete sealing.

Please refer to FIG. 3 , which is a three-dimensional view of the top cover of the micro-tube placed in the micro-porous plate according to the present invention. As shown in the figure, in the operating embodiment of the present invention, a plurality of microtube upper covers 2 are placed on the microporous plate 4 respectively.

Please refer to FIG. 4 and FIG. 5 at the same time, which are a perspective view and a cross-sectional view of the drive device of the present invention connected to the upper cover of the microtube. As shown in the figure, the rod body 12 of the driving device 1 of the present invention extends into the embedding groove 23 of the microtube upper cover 2, so that the microtube upper cover 2 is engaged with the lower end of the rod body 12, and its upper edge is held against it The positioning portion 13 at the bottom end of the driving device 1 is then mechanically moved to extract the microtube top cover 2 and move to the top of the target microtube container.

Please refer to FIG. 6 and FIG. 7 at the same time, which are a perspective view and a cross-sectional view of the microtube upper cover connected to the microtube container of the present invention. Please cooperate with FIG. 2. As shown in the figure, in the present invention, after the rod body 12 of the driving device 1 engages with the microtube upper cover 2 and moves to the top of the microtube container 3 on the target microtube holder 5, the driving device 1 passes through the microtube container 3. Snap the microtube upper cover 2 to the microtube container 3 downward. Wherein, since the inner edge of the wall 22 of the microtube upper cover 2 is a clamping groove 24, the opening of the microtube container 3 can be embedded in the clamping groove 24 and tightly combined with the microtube upper cover 2, thereby sealing the the biological sample.

Please refer to FIG. 8 and FIG. 9 at the same time, in conjunction with FIG. 10 , which are the moving state diagram of the present invention, as well as the three-dimensional view and cross-sectional view of placing the micro-tube container on the micro-tube rack. As shown in the figure, after the microtube upper cover 2 is covered in the present invention, the mechanical movement of the present invention directly extracts the microtube container 3 that is closely combined with the microtube upper cover 2, and mechanically moves the microtube container 3 from the microtube container 3. Rack 5 is moved to another microtube rack 5a. Thereby, the present invention can simultaneously perform the steps of capping and moving in a single mechanical movement, simplifying tedious steps and mechanical settings, making the automated processing process more efficient, and reducing the development cost of automated instruments.

Please refer to FIG. 11 and FIG. 12 at the same time, in conjunction with FIG. 13 , which are the action diagrams of disassembling the upper cover of the microtube, as well as the perspective view and cross-sectional view of disassembling the upper cover of the microtube according to the present invention. As shown in the figure, in the present invention, after the microtube container 3 is moved from the microtube holder 4 to another microtube holder 5a, the driving device 1 drives the rod body 12 to move upward. The positioning part 13 is held on the bottom end of the driving device 1. When the rod body 12 moves upward, the lower end of the rod body 12 will be separated from the upper cover 2 of the microtube. on the tube rack 5a for subsequent detection steps.

Please refer to FIG. 14 , which is a perspective view of another operating embodiment of the present invention. As shown in the figure, another operating embodiment of the present invention, in which the driving device 1 and the microtube upper cover 2 have the same way of removing the cover and the upper cover as described above, which will not be repeated here. The only difference in this embodiment is that the microtube container is 3 is changed to micro-porous plate 4. The microporous disc 4 is a disc-shaped structure and is provided with a plurality of microwells for loading the biological sample. In the driving device 1 of the present invention, the upper cover 2 of the micro-tube can be directly covered on the micro-well of the micro-porous disc and tightly combined with it. Furthermore, through the movement of the driving device 1, the micro-porous disk 4 tightly combined with the micro-tube cover 2 can be directly extracted, and the micro-porous disk 4 can be moved to a specific position as needed.

The foregoing examples are only to illustrate the preferred embodiments of the present invention, but not to limit the scope of the present invention. All modifications and changes are made without losing the essence of the present invention and without departing from the spirit and scope of the present invention.

To sum up, the present invention uses a driving device with a positioning portion on the bottom edge, a rod body that can move up and down on the driving device, and a microtube upper cover detachably connected to the rod body to form a microtube upper cover and its assembly device. It is used to seal the cap of the microtube to the microtube container and move it to different microtube racks for automatic biological sample preparation, analysis and inspection, avoiding the leakage risk of incomplete sealing and making the automatic processing process more efficient. It is a practical design, and it is a creation of novelty. I should file a patent application in accordance with the law, and I pray that the bureau will examine it and grant the patent as soon as possible.

1: drive unit 11: Slide rail 111: Limit slot 12: Rod body 121: Kakibe 13: Positioning part 131: Screw part 2: upper cover 21: Covering part 22: Kakibe 23: Embedded slot 24: clip slot 3: Microtube container 4: Micro-well plate 5: Micro tube rack 5a: Micro tube rack

FIG. 1 is an exploded perspective view of the present invention. Figure 2 is a cross-sectional view of the present invention. Fig. 3 is a perspective view of the top cover of the microtube of the present invention placed on a microporous plate. 4 is a perspective view of the drive device of the present invention connected to the top cover of the microtube. FIG. 5 is a cross-sectional view of the drive device of the present invention connected to the top cover of the microtube. FIG. 6 is a perspective view of the microtube upper cover connected to the microtube container according to the present invention. FIG. 7 is a cross-sectional view of the microtube top cover connected to the microtube container according to the present invention. FIG. 8 is a moving state diagram of the present invention. FIG. 9 is a perspective view of placing the microtube container in the microtube rack according to the present invention. Fig. 10 is a cross-sectional view of placing the microtube container in the microtube rack according to the present invention. Fig. 11 is a diagram showing the action of disassembling the upper cover of the microtube according to the present invention. Fig. 12 is a perspective view of disassembling the upper cover of the microtube according to the present invention. Fig. 13 is a cross-sectional view of disassembling the upper cover of the microtube according to the present invention. FIG. 14 is a perspective view of another operating embodiment of the present invention.

1: drive unit

11: Slide rail

111: Limit slot

12: Rod body

121: Kakibe

13: Positioning part

131: Screw part

2: upper cover

21: Covering part

22: Kakibe

23: Embedded slot

24: clip slot

3: Microtube container

Claims (6)

A microtube top cover and its assembling device, comprising at least a microtube top cover and a driving device, wherein the microtube top cover has a cover part for sealing the microtube container, and a can be formed with a driving device A detachable connecting groove; the driving device is provided with a positioning part, and a rod body that can move up or down along the driving device and the positioning part for being embedded in the embedding groove of the upper cover of the microtube, The upper cover of the micropipette is made to form a detachable connection. The assembly structure of the microtube top cover according to claim 1, wherein the driving device is provided with a slide rail, and a rod body is provided on the slide rail, so that the rod body can move up or down along the slide rail. The microtube top cover and its assembling device as claimed in claim 1, wherein the slide rail of the driving device is provided with a limiting groove of appropriate length, and the rod system is provided with a horizontal protrusion at an appropriate position and is located at the limiting position The wall portion of the groove is used to limit the position of the rod body through the limiting groove when the rod body moves up and down along the slide rail. The microtube top cover and its assembling device as claimed in claim 1, wherein the positioning portion provided in the driving device is in the shape of a sleeve, and the upper edge thereof is provided with a locking portion, through which the locking portion is locked into the microtube The sliding rail is fixed on the bottom end of the driving device. The microtube top cover and its assembling device as claimed in claim 1, wherein the top edge of the cover portion of the microtube top cover is extended horizontally and vertically outward to form a clamping groove, so that the opening of the microtube container is just right It can be embedded in the clip groove and tightly combined with the upper cover of the microtube. The microtube top cover and its assembling device as claimed in claim 1, wherein the cover portion of the microtube top cover is transparent.

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