CN113667591A - Full-automatic nucleic acid detection and analysis system - Google Patents
Full-automatic nucleic acid detection and analysis system Download PDFInfo
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- CN113667591A CN113667591A CN202010413278.7A CN202010413278A CN113667591A CN 113667591 A CN113667591 A CN 113667591A CN 202010413278 A CN202010413278 A CN 202010413278A CN 113667591 A CN113667591 A CN 113667591A
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Abstract
The invention provides a full-automatic nucleic acid detection and analysis system, which comprises a detection kit, a conveying mechanism for driving the detection kit to move, a nucleic acid extraction module, a nucleic acid amplification module and a fluorescence detection module; the conveying mechanism drives the detection kit to respectively reach the nucleic acid extraction module for nucleic acid extraction, the nucleic acid amplification module for amplification and the fluorescence detection module for detection; the detection kit is provided with a spe pool, a sample pool, a control chamber, a liquid path, an amplification reaction area and a to-be-detected area, wherein the liquid path is communicated with the spe pool, the sample pool, the amplification reaction area and the to-be-detected area, the control chamber is positioned above the liquid path, the control chamber is provided with a valve, and magnetic stirring components are arranged in the spe pool and the sample pool. By adopting the technical scheme of the invention, the processes of nucleic acid extraction, nucleic acid amplification, fluorescence detection and the like are integrated into a whole, the operation process is simple, no manual operation is needed in the detection process, and micro integration and rapid automation are realized.
Description
Technical Field
The invention belongs to the technical field of nucleic acid detection equipment, and particularly relates to a full-automatic nucleic acid detection and analysis system.
Background
The nucleic acid detection is a technique for detecting DNA by blood, other body fluids or cells, which is to take tissue cells of a subject, amplify the gene information, detect DNA molecular information in the cells by a specific device, analyze the gene condition contained in the DNA molecular information, and diagnose diseases.
Since a sample generally used for nucleic acid detection is a biological tissue cell, it is necessary to perform steps such as cell lysis, nucleic acid purification, nucleic acid amplification, and the like, and then detect nucleic acid by an optical detection method. Because different processing methods are involved in each step, a sample needs to be processed for multiple times through multiple devices respectively, meanwhile, professional experimenters need to perform complex operations of the various devices, samples among the devices need to be transferred mutually, and the processes are easy to consume time and labor, easily introduce pollution and influence the result of nucleic acid detection.
Disclosure of Invention
Aiming at the technical problems, the invention discloses a full-automatic nucleic acid detection and analysis system, which organically integrates the processes of nucleic acid extraction, nucleic acid amplification, fluorescence detection and the like, has quick and efficient operation, prevents cross contamination because all biological reaction processes are carried out in a nucleic acid detection kit, and can realize automatic control through a control module.
In contrast, the technical scheme adopted by the invention is as follows:
a full-automatic nucleic acid detection and analysis system comprises a detection kit, a conveying mechanism for driving the detection kit to move, a nucleic acid extraction module, a nucleic acid amplification module and a fluorescence detection module;
the conveying mechanism drives the detection kit to respectively reach the nucleic acid extraction module for nucleic acid extraction, the nucleic acid amplification module for amplification and the fluorescence detection module for detection;
the detection kit is provided with a solid phase extraction pool, namely a spe pool, a sample pool, a control room, a liquid path, an amplification reaction area and a to-be-detected area, wherein the liquid path is communicated with the spe pool, the sample pool, the amplification reaction area and the to-be-detected area;
the nucleic acid extraction module comprises a driving mechanism, a spe pool heating and mixing assembly, a sample pool mixing assembly, a gas path interface assembly and a lifting mechanism, wherein the driving mechanism is positioned above the conveying mechanism, and the spe pool heating and mixing assembly and the sample pool mixing assembly are positioned below the conveying mechanism and are respectively connected with the lifting mechanism; the spe pool heating and mixing assembly and the sample pool mixing assembly both comprise magnetic rotating mechanisms;
the driving mechanism is connected with a pressure rod, the position of the pressure rod corresponds to that of a control chamber of the detection kit, the driving mechanism drives the pressure rod to move up and down and controls the circulation of a liquid path through a valve, the spe pool heating and mixing assembly corresponds to the position of a spe pool, and the position of the sample pool mixing assembly corresponds to that of a sample pool;
the detection kit is provided with a connecting interface communicated with the liquid path, one end of the gas path interface component is connected with a gas pipe, and the other end of the gas path interface component is provided with a gas path interface which can be connected with the connecting interface;
the conveying mechanism drives the detection kit to move, and simultaneously, the following actions are carried out: the driving motor extends into a reagent chamber in the detection kit through the driving pressure rod and controls the state of a valve of the reagent chamber, so that liquid in the liquid path flows into the spe pool and the sample pool, and the gas path interface component is connected with the connecting interface and drives the liquid in the liquid path to flow; the lifting mechanism drives the spe pool heating and mixing assembly and the sample pool mixing assembly to be respectively close to the bottoms of the spe pool and the sample pool, the spe pool is heated and mixed, and the sample pool is mixed.
As a further improvement of the invention, the bottom of the control chamber is provided with a through hole, the valve is a deformable valve gasket positioned at the bottom of the control chamber, the valve gasket is deformed by pressing the valve gasket through a pressure rod, and the valve gasket extends into a liquid path below the valve gasket through the through hole to cut off the liquid path. And a spring and a silica gel sealing ring are arranged on the gas path connector.
As a further improvement of the invention, the nucleic acid extraction module comprises a pump, an electromagnetic valve group and an air pipe connected with a pump valve interface, wherein the pump is connected with the air pipe through the electromagnetic valve group, and the air pipe is connected with one end of an air passage interface component.
As a further improvement of the invention, the gas path interface is provided with a spring and a silica gel sealing ring, and when the gas path interface is connected with the connection interface of the test kit, the interface is reliably sealed.
As a further improvement of the invention, the driving mechanism comprises a cam assembly and a driving motor, and the driving motor is connected with the pressure rod through the cam assembly. Further, the cam assembly comprises a plurality of groups of cams.
Further, conveying mechanism, driving motor, spe pond heating and mixing subassembly, sample cell mixing subassembly, pump, electromagnetism valves etc. all are connected with control module, realize automatic control through control module, can realize automatic nucleic acid extraction.
As a further improvement of the invention, the nucleic acid amplification module comprises a thermal cycling component, a pressing block and a lifting driving mechanism, wherein the pressing block is positioned above the test kit, the thermal cycling component is positioned below the test kit, and the lifting driving mechanism is connected with the thermal cycling component and drives the thermal cycling component to be attached to or separated from an amplification reaction zone of the detection kit.
As a further improvement of the present invention, the thermal cycle means comprises a heating/cooling means, a heat transfer plate for transferring heat in contact with the amplification reaction zone of the detection kit 1, and the heating/cooling means is connected to the heat transfer plate.
As a further improvement of the present invention, the thermal cycle component includes a temperature control plate, a heat sink, a heat dissipation fan, and a temperature control probe, the temperature control plate is electrically connected to the heat sink, the heat dissipation fan, the temperature control probe, and the heating/cooling member, respectively, and the temperature control probe is in contact with the heat sink and the heating/cooling member.
Furthermore, the heating/refrigerating component, the lifting driving mechanism, the temperature control plate, the cooling fan and the temperature control probe are all connected with the control module, automatic control is achieved through the control module, and automatic amplification can be achieved.
As a further improvement of the invention, the fluorescence detection module comprises an emission light unit, a lens group unit and a receiving detection unit, wherein light emitted by the emission light unit is reflected to a region to be detected on the kit through the lens group unit, a nucleic acid sample in the region to be detected emits a fluorescence signal after being excited by incident light, and the fluorescence signal enters the receiving detection unit for imaging after passing through the lens group unit.
As a further improvement of the present invention, the emission light unit includes a light source, the lens group unit includes a light uniformizing member, a light filter, a dichroic mirror and a total reflection mirror, the light uniformizing member is located on an emission light path of the light source, the light filter is located at the front end of the light uniformizing member, the dichroic mirror is located at the front end of the light filter, the total reflection mirror is located at one side of the dichroic mirror, and a reflection surface of the total reflection mirror and an emission surface of the dichroic mirror form an acute angle; the total reflector is positioned above the test kit, and the reflecting surface of the total reflector and the test kit are arranged in an acute angle;
the receiving detection unit comprises a focusing mechanism, the front end of the focusing component is provided with a receiving optical filter, and the reflecting surface of the total reflector faces the receiving optical filter;
incident light emitted by the light source is uniformly modulated by the light homogenizing component, is irradiated to the dichroic mirror after being filtered by the optical filter, light beams are reflected by the dichroic mirror and enter the total reflector to be reflected to a region to be detected on the kit, nucleic acid samples loaded on the region to be detected are excited by the incident light to emit fluorescent signals, the fluorescent signals are reflected to the dichroic mirror by the total reflector, the fluorescent light penetrates through the dichroic mirror, enters the receiving optical filter to be filtered to remove stray light, and enters the receiving detection unit through the focusing component to be imaged.
The fluorescence detection module comprises a fluorescence image analysis module used for receiving the pictures imaged by the detection unit for analysis.
As a further improvement of the invention, the reflecting surface of the dichroic mirror forms an included angle of 45 degrees with the light path of the light source; the total reflection mirror and the reflection surface of the dichroic mirror form an included angle of 45 degrees.
As a further improvement of the invention, the dodging component is an optical lens, a lens group or an optical fiber.
As a further improvement of the present invention, the receiving and detecting unit is a camera or a photomultiplier tube.
As a further improvement of the present invention, the focusing member is a focusing lens or a pinhole mechanism.
As a further improvement of the invention, the area to be detected is provided with a biochip, the biochip is provided with a plurality of dot matrix reaction tanks, and index detection reagents are filled in the dot matrix reaction tanks.
Compared with the prior art, the invention has the beneficial effects that:
firstly, by adopting the technical scheme of the invention, the processes of nucleic acid extraction, nucleic acid amplification, fluorescence detection and the like are integrated, and a plurality of indexes can be simultaneously detected by matching with a multi-index biochip, so that the synchronous automatic detection of the plurality of indexes is finally realized. The operation process is simple, no manual operation is needed in the inspection process, and micro integration and rapid automation are achieved. Moreover, all biological reactions are carried out in the detection kit, so that the full-automatic nucleic acid detection and analysis system and the laboratory environment are not polluted, and the detection can be carried out without a special PCR laboratory.
Secondly, the technical scheme of the invention greatly shortens the detection time, and can further automatically analyze and simultaneously give out a multi-index detection result by carrying the automatic analysis module, help a doctor to quickly and accurately identify the pathogenic genes and guide the doctor to take medicine in a targeted manner in time. The system can integrate all functions of gene detection, does not need to purchase equipment with different operations, reduces purchase cost and saves laboratory space.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a fully automatic nucleic acid detection and analysis system according to an embodiment of the present invention.
FIG. 2 is a schematic exploded view of a nucleic acid extraction module according to an embodiment of the present invention.
FIG. 3 is a schematic view showing a state where a valve of the test kit according to the embodiment of the present invention is opened.
FIG. 4 is a schematic view showing a state where a valve of the test kit according to the embodiment of the present invention is closed.
FIG. 5 is a schematic view of the test kit according to the embodiment of the present invention controlling the transfer of the reagent by the plunger.
FIG. 6 is a schematic view of a sample cell of the test kit according to the embodiment of the present invention.
FIG. 7 is a schematic structural diagram of a nucleic acid amplification module according to an embodiment of the present invention.
FIG. 8 is a schematic structural diagram of a fluorescence detection module according to an embodiment of the present invention.
FIG. 9 is a schematic structural view of a biochip according to an embodiment of the present invention.
The reference numerals include:
100-detection kit, 200-nucleic acid extraction module, 300-nucleic acid amplification module, 400-fluorescence detection module;
11-a sample cell, 12-a liquid path, 13-a control chamber, 14-a valve, 15-a magnetic stirring rod,
21-a cam assembly, 22-a spe pool heating and mixing assembly, 23-a sample pool mixing assembly, 24-an air channel interface assembly, 25-a pump, 26-an electromagnetic valve group and 27-a pressure rod;
31-thermal cycle parts, 32-briquettes;
41-light source, 42-light homogenizing component, 43-optical filter, 44-dichroic mirror, 45-total reflection mirror, 46-detection area to be detected, 47-receiving optical filter, 48-focusing lens, 49-receiving detection unit and 50-biochip.
Detailed Description
Preferred embodiments of the present invention are described in further detail below.
Example 1
As shown in fig. 1, a full-automatic nucleic acid detecting and analyzing system includes a detection kit 100, a conveying mechanism for driving the detection kit 100 to move, a nucleic acid extracting module 200, a nucleic acid amplifying module 300, and a fluorescence detecting module 400; the conveying mechanism drives the detection kit 100 to respectively reach the nucleic acid extraction module 200 for nucleic acid extraction, the nucleic acid amplification module 300 for amplification and the fluorescence detection module 400 for detection.
As shown in fig. 2 to 6, the detection kit 100 is provided with a spe pool, a sample pool 11, a control chamber 13, a liquid path 12, an amplification reaction region and a region to be detected 46, the liquid path 12 is communicated with the spe pool, the sample pool 11, the amplification reaction region and the region to be detected 46, the control chamber 13 is located above the liquid path 12, the control chamber 13 is provided with a valve 14, and the spe pool and the sample pool 11 are internally provided with a magnetic stirring rod 15.
As shown in fig. 2, the nucleic acid extraction module 200 includes a driving mechanism, a spe pool heating and mixing assembly 22, a sample pool mixing assembly 23, an air channel interface assembly 24, and a lifting mechanism, wherein the driving mechanism is located above the conveying mechanism, and the spe pool heating and mixing assembly 22 and the sample pool mixing assembly 23 are located below the conveying mechanism and are respectively connected to the lifting mechanism; the spe cell heating and mixing component 22 and the sample cell mixing component 23 both comprise magnetic rotating mechanisms; the driving mechanism is connected with a pressure lever 27, the pressure lever 27 corresponds to the position of the control chamber 13 of the detection kit 100, the driving mechanism drives the pressure lever 27 to move up and down, the circulation of the liquid path 12 is controlled through a valve 14, the spe cell heating and mixing assembly 22 corresponds to the spe cell, and the sample cell mixing assembly 23 corresponds to the sample cell 11; the detection kit 100 is provided with a connection interface communicated with the liquid path 12, one end of the gas path interface component 24 is connected with a gas pipe, and the other end of the gas path interface component 24 is provided with a gas path interface which can be connected with the connection interface. The drive mechanism includes a cam assembly 21 and a drive motor connected to a strut 27 through the cam assembly 21.
The conveying mechanism drives the detection kit 100 to move, and simultaneously, the following actions are carried out: the driving motor extends into a reagent chamber in the detection kit 100 through the driving pressure rod 27, and controls the state of a valve 14 of the reagent chamber, so that liquid in the liquid path 12 flows into the spe cell and the sample cell, and the gas path interface component 24 is connected with the connecting interface to drive the liquid in the liquid path 12 to flow; elevating system drives spe pond heating and mixing subassembly 22, sample cell mixing subassembly 23 and presses close to the bottom of spe pond and sample cell 11 respectively, heats the spe pond and mixes, mixes the sample cell, realizes handling reagent and the sample in the corresponding region in nucleic acid detect reagent box 100, realizes functions such as heating, mixing and magnetic bead absorption. As shown in fig. 6, the motor of the sample cell mixing assembly drives the magnet to rotate, and the magnetic stirring rod 15 of the sample cell in the nucleic acid detection kit 100 rotates along with the magnet, so as to realize the function of uniform mixing; when the magnetic beads are arranged in the nucleic acid detection kit 100, the magnet on the instrument can be lifted to realize the adsorption and release of the magnetic beads.
As shown in fig. 3 and 4, the bottom of the control chamber 13 is provided with a through hole, the valve 14 is a deformable valve gasket located at the bottom of the control chamber 13, the valve 14 gasket is deformed by pressing the valve 14 gasket through a pressing rod 27, and the valve 14 gasket extends into the liquid path 12 below through the through hole to cut off the liquid path 12.
The nucleic acid extraction module 200 realizes the ordered movement of the sample and the reagent in the detection kit by the precise control and the direction switching of the liquid path 12 in the detection kit 100, and completes the processes of cell lysis, nucleic acid purification, amplification sample introduction and the like required by nucleic acid extraction.
The cam assembly 21 consists of a cam pack consisting of a number of specially designed cams. When the driving motor drives the cam group to rotate to different angles, each cam is also positioned at different angles, and the edge of each cam is a curve profile, so that the corresponding pressure lever 27 can be pressed to different heights, and the on-off of each reaction chamber in the chip and the reagent sample introduction process can be controlled by matching with the internal structure of the nucleic acid detection kit 100.
The sample introduction principle of the reagent is as follows: as shown in FIG. 5, in cooperation with the plunger structure inside the nucleic acid detecting reagent kit 100, when the cam member 21 rotates to different angles, the pressing rod 27 is gradually pressed down, so as to control the controlled transfer of the corresponding reagents inside the reagent kit.
The nucleic acid extraction module 200 comprises a pump 25, an electromagnetic valve group 26 and an air pipe connected with a valve interface of the pump 25, wherein the pump 25 is connected with the air pipe through the electromagnetic valve group 26, and the air pipe is connected with one end of an air passage interface component 24; the gas circuit interface assembly 24, the pump 25, the solenoid valve set 26 and the gas pipe connected with the valve interface of the pump 25 provide a power source for the flow of liquid in the detection kit 100, so that samples and reagents are transferred in the kit in order. And a spring and a silica gel sealing ring are arranged on the gas path interface, so that the reliable sealing of the gas path interface assembly 24 and the interface of the detection kit 100 is ensured.
When the detection kit 100 is placed into the full-automatic nucleic acid detection and analysis system, the conveying mechanism drives the detection kit 100 to move, reliably positions the detection kit with the positions of the pressure rods 27 of the cam assembly 21, reliably seals the gas path interface assembly 24, and tightly contacts the spe cell heating and mixing assembly 22 and the sample cell mixing assembly 23 to ensure effective mixing and heating conduction.
At present, a DNA extractor is used for extracting nucleic acid in the market, a traditional extraction method is adopted, after extraction is finished in a PE tube, the nucleic acid is added into an amplification instrument in the next step in a manual liquid transfer mode, and cross contamination is easy to generate.
This nucleic acid extraction module 200 cooperates corresponding detect reagent box 100, carries out the accurate orderly control of flow path to the kit, has carried out a series of automatic operation such as mixture, schizolysis, washing, rinsing, appearance introduction with the sample in the reagent box, has avoided the artificial operation process of traditional instrument, operates high efficiency, full automation, and all biological reaction processes all go on in nucleic acid detect reagent box 100, have prevented cross contamination.
Example 2
In addition to example 1, as shown in fig. 7, the nucleic acid amplification module 300 includes a thermal cycling part 31, a pressing block 32, and a lifting/lowering driving mechanism, wherein the pressing block 32 is located above the test kit, the thermal cycling part 31 is located below the test kit, and the lifting/lowering driving mechanism is connected to the thermal cycling part 31 to drive the thermal cycling part 31 to be attached to or detached from the amplification reaction region of the detection kit 100. The thermal cycling part 31 can provide a rapid temperature change environment for nucleic acid amplification, and the cooling fins in the thermal cycling part 31 heat or cool the nucleic acid and uniformly transfer the heated nucleic acid to the amplification reaction area of the detection kit 100 through the heating plate.
The thermal cycling part 31 includes a heating/cooling means, a heat transfer plate for transferring heat in contact with the amplification reaction region of the detection kit 100, the heating/cooling means being connected to the heat transfer plate; the thermal cycle unit 31 further includes a temperature control plate, a heat sink, a heat dissipation fan, and a temperature control probe, wherein the temperature control plate is electrically connected to the heat sink, the heat dissipation fan, the temperature control probe, and the heating/cooling member, respectively, and the temperature control probe is in contact with the heat sink and the heating/cooling member. The temperature control plate detects the temperature of the heating/refrigerating component and the cooling fin through the temperature control probe, adjusts the heating amount or the refrigerating amount of the heating/refrigerating component in real time, adjusts the air volume of the cooling fan, and accurately controls the temperature of the amplification reaction zone. When the detection kit 100 is conveyed to the nucleic acid amplification module 300, the thermal cycling component 31 is driven by the lifting driving mechanism to rise and tightly attach to the amplification reaction area of the detection kit 100, so that the temperature is rapidly and uniformly transmitted to the whole nucleic acid amplification reaction area of the detection kit 100. By controlling the temperature of the thermal cycler unit 31, a suitable temperature environment can be provided for amplification of nucleic acids in the detection kit 100.
Example 3
On the basis of embodiment 2, as shown in fig. 8, the fluorescence detection module 400 includes an emission light unit, a lens group unit, and a receiving detection unit 49, light emitted by the emission light unit is reflected to the region to be detected 46 on the kit through the lens group unit, and a nucleic acid sample in the region to be detected 46 emits a fluorescence signal after being excited by incident light, and enters the receiving detection unit 49 to be imaged after passing through the lens group unit.
The light emitting unit includes a light source 41, and the light source 41 may be an LED lamp or a laser with various wavelengths. The lens group unit comprises a light homogenizing component 42, a light filter 43, a dichroic mirror 44 and a total reflection mirror 45, wherein the light homogenizing component 42 is positioned on a light emitting path of the light source 41, the light filter 43 is positioned at the front end of the light homogenizing component 42, the dichroic mirror 44 is positioned at the front end of the light filter 43, the total reflection mirror 45 is positioned at one side of the dichroic mirror 44, and a reflecting surface of the total reflection mirror 45 and the light emitting surface of the dichroic mirror 44 form an acute angle; the total reflector 45 is positioned above the test kit, and the reflecting surface of the total reflector 45 and the test kit are arranged in an acute angle; the receiving detection unit 49 comprises a focusing lens 48, a receiving filter 47 is arranged at the front end of the focusing lens 48, and the reflecting surface of the total reflection mirror 45 faces the receiving filter 47. The reflecting surface of the dichroic mirror 44 forms an included angle of 45 degrees with the light path of the light source 41, and the reflecting surface of the total reflection mirror 45 forms an included angle of 45 degrees with the reflecting surface of the dichroic mirror 44; the light homogenizing component 42 is an optical lens, a lens group or an optical fiber; the receiving and detecting unit 49 is a camera or a photomultiplier tube.
Incident light emitted by a light source 41 is uniformly modulated by a light homogenizing component 42, after stray light is filtered by a light filter 43, light beams pass through a dichroic mirror 44 which forms an angle of 45 degrees with a light path, because the wavelength of the incident light is within the reflection range of the dichroic mirror 44, the light beams enter a total reflection mirror 45 which forms an angle of 45 degrees with the light path after being reflected to a region 46 to be detected on a kit, a nucleic acid sample loaded on the region 46 to be detected is excited by the incident light, a fluorescent signal is emitted, the fluorescent signal is reflected to the dichroic mirror 44 by the total reflection mirror 45, and because the wavelength of the fluorescent signal is within the transmission range of the dichroic mirror 44, the fluorescent light penetrates through the dichroic mirror 44, enters a receiving light filter 47 to filter the stray light, and enters a receiving detection unit 49 through a focusing lens 48 to be imaged. The fluorescence detection module 400 includes a fluorescence image analysis module for receiving and analyzing the image imaged by the detection unit 49, and through analysis of the fluorescence image, fluorescence intensity signals of all regions of the to-be-detected region 46 of the kit can be obtained.
Further, treat that detection zone 46 is equipped with biochip 50, be equipped with a plurality of dot matrix reaction tanks on biochip 50, be equipped with different index detect reagent in the dot matrix reaction tank, mutually independent, each other does not cross talk. A schematic diagram of the biochip 50 is shown in FIG. 9.
Furthermore, conveying mechanism, driving motor, spe pond heating and mixing subassembly, sample pond mixing subassembly, pump, electromagnetism valves, heating/refrigeration component, lift actuating mechanism, temperature control plate, radiator fan, control by temperature change probe etc. all are connected with control module, realize automatic control through control module, can realize automatic nucleic acid extraction, amplification and analysis.
All reaction tanks independently amplify in airtight space during nucleic acid amplification, the exciting light is launched in real time to the fluorescence detection system of the instrument in the amplification process, excite all reaction tanks to give out fluorescence, the instrument filters the fluorescence and gathers, carry out multizone synchronous processing according to the distribution of reaction tanks, draw the real-time detection curve of a plurality of dot matrix reaction tanks simultaneously, give the testing result of each index respectively according to the data of this curve in whole amplification process, finally realize the quick automated inspection of many indexes.
The foregoing is a more detailed description of the invention in connection with specific preferred embodiments and it is not intended that the invention be limited to these specific details. For those skilled in the art to which the invention pertains, several simple deductions or substitutions can be made without departing from the spirit of the invention, and all shall be considered as belonging to the protection scope of the invention.
Claims (10)
1. A full-automatic nucleic acid detection and analysis system is characterized in that: the kit comprises a detection kit, a conveying mechanism for driving the detection kit to move, a nucleic acid extraction module, a nucleic acid amplification module and a fluorescence detection module;
the conveying mechanism drives the detection kit to respectively reach the nucleic acid extraction module for nucleic acid extraction, the nucleic acid amplification module for amplification and the fluorescence detection module for detection;
the detection kit is provided with a spe pool, a sample pool, a control chamber, a liquid path, an amplification reaction area and a to-be-detected area, wherein the liquid path is communicated with the spe pool, the sample pool, the amplification reaction area and the to-be-detected area;
the nucleic acid extraction module comprises a driving mechanism, a spe pool heating and mixing assembly, a sample pool mixing assembly, a gas path interface assembly and a lifting mechanism, wherein the driving mechanism is positioned above the conveying mechanism, and the spe pool heating and mixing assembly and the sample pool mixing assembly are positioned below the conveying mechanism and are respectively connected with the lifting mechanism; the spe pool heating and mixing assembly and the sample pool mixing assembly both comprise magnetic rotating mechanisms;
the driving mechanism is connected with a pressure rod, the position of the pressure rod corresponds to that of a control chamber of the detection kit, the driving mechanism drives the pressure rod to move up and down and controls the circulation of a liquid path through a valve, the spe pool heating and mixing assembly corresponds to the position of a spe pool, and the position of the sample pool mixing assembly corresponds to that of a sample pool;
the detection kit is provided with a connecting interface communicated with the liquid path, one end of the gas path interface component is connected with a gas pipe, and the other end of the gas path interface component is provided with a gas path interface which can be connected with the connecting interface;
the conveying mechanism drives the detection kit to move, and simultaneously, the following actions are carried out: the driving motor extends into a reagent chamber in the detection kit through the driving pressure rod and controls the state of a valve of the reagent chamber, so that liquid in the liquid path flows into the spe pool and the sample pool, and the gas path interface component is connected with the connecting interface and drives the liquid in the liquid path to flow; the lifting mechanism drives the spe pool heating and mixing assembly and the sample pool mixing assembly to be respectively close to the bottoms of the spe pool and the sample pool, the spe pool is heated and mixed, and the sample pool is mixed.
2. The fully automated nucleic acid detection and analysis system of claim 1, wherein: the bottom of the control chamber is provided with a through hole, the valve is a deformable valve gasket positioned at the bottom of the control chamber, the valve gasket is extruded by the pressing rod to deform, and the valve gasket extends into a liquid path below the valve gasket through the through hole to cut off the liquid path.
3. The fully automated nucleic acid detection and analysis system according to claim 2, wherein: the nucleic acid extraction module comprises a pump, an electromagnetic valve group and an air pipe connected with a pump valve interface, wherein the pump is connected with the air pipe through the electromagnetic valve group, and the air pipe is connected with one end of an air passage interface component; and a spring and a silica gel sealing ring are arranged on the gas path connector.
4. The fully automated nucleic acid detection and analysis system of claim 3, wherein: the driving mechanism comprises a cam assembly and a driving motor, and the driving motor is connected with the pressure rod through the cam assembly.
5. The fully automated nucleic acid detection and analysis system of claim 1, wherein: the nucleic acid amplification module comprises a thermal cycle component, a pressing block and a lifting driving mechanism, wherein the pressing block is positioned above the test kit, the thermal cycle component is positioned below the test kit, and the lifting driving mechanism is connected with the thermal cycle component and drives the thermal cycle component to be attached to or separated from an amplification reaction zone of the detection kit.
6. The fully automated nucleic acid detection and analysis system of claim 4, wherein: the thermal cycling component comprises a heating/cooling component and a heat transfer plate used for contacting with the amplification reaction zone of the detection kit 1 to transfer heat, and the heating/cooling component is connected with the heat transfer plate;
the thermal cycle part also comprises a temperature control plate, a radiating fin, a radiating fan and a temperature control probe, wherein the temperature control plate is respectively electrically connected with the radiating fin, the radiating fan, the temperature control probe and the heating/refrigerating member, and the temperature control probe is contacted with the radiating fin and the heating/refrigerating member.
7. The fully automatic nucleic acid detection and analysis system according to any one of claims 1 to 6, wherein: the fluorescence detection module comprises an emitting light unit, a lens group unit and a receiving detection unit, light emitted by the emitting light unit is reflected to a region to be detected on the kit through the lens group unit, a nucleic acid sample in the region to be detected is excited by incident light to send a fluorescence signal, and the fluorescence signal enters the receiving detection unit to be imaged after passing through the lens group unit.
8. The fully automated nucleic acid detection and analysis system of claim 7, wherein: the light emitting unit comprises a light source, the lens group unit comprises a light homogenizing component, a light filter, a dichroic mirror and a total reflection mirror, the light homogenizing component is positioned on a light emitting path of the light source, the light filter is positioned at the front end of the light homogenizing component, the dichroic mirror is positioned at the front end of the light filter, the total reflection mirror is positioned on one side of the dichroic mirror, and a reflecting surface of the total reflection mirror and an emitting surface of the dichroic mirror form an acute angle; the total reflector is positioned above the test kit, and the reflecting surface of the total reflector and the test kit are arranged in an acute angle;
the receiving detection unit comprises a focusing mechanism, the front end of the focusing component is provided with a receiving optical filter, and the reflecting surface of the total reflector faces the receiving optical filter;
incident light emitted by a light source is uniformly modulated by a light homogenizing component, is filtered by a light filter and then is emitted to a dichroic mirror, light beams are reflected by the dichroic mirror and then enter a total reflector to be reflected to a region to be detected on a kit, a nucleic acid sample loaded on the region to be detected is excited by the incident light, then a fluorescence signal is emitted, is reflected to the dichroic mirror by the total reflector, and the fluorescence penetrates through the dichroic mirror, enters a receiving light filter to be filtered to remove stray light and enters a receiving detection unit through a focusing component to be imaged;
the fluorescence detection module comprises a fluorescence image analysis module used for receiving the pictures imaged by the detection unit for analysis.
9. The fully automated nucleic acid detection and analysis system of claim 8, wherein: the reflecting surface of the dichroic mirror forms a 45-degree included angle with the light path of the light source, and the total reflecting mirror forms a 45-degree included angle with the reflecting surface of the dichroic mirror; the light homogenizing component is an optical lens, a lens group or an optical fiber; the receiving and detecting unit is a camera or a photomultiplier;
the focusing component is a focusing lens or a pinhole mechanism.
10. The fully automated nucleic acid detection and analysis system of claim 9, wherein: treat that the detection zone is equipped with biochip, be equipped with a plurality of dot matrix reaction ponds on the biochip, be equipped with index detect reagent in the dot matrix reaction pond.
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| CN202010413278.7A CN113667591A (en) | 2020-05-15 | 2020-05-15 | Full-automatic nucleic acid detection and analysis system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115290567A (en) * | 2022-09-29 | 2022-11-04 | 儒克生物科技常州有限公司 | Sealed conveying system for fluorescence detection and working method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115290567A (en) * | 2022-09-29 | 2022-11-04 | 儒克生物科技常州有限公司 | Sealed conveying system for fluorescence detection and working method thereof |
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