WO2021026832A1

WO2021026832A1 - Microburette upper lid, kit comprising the microburette upper lid and method thereof

Info

Publication number: WO2021026832A1
Application number: PCT/CN2019/100637
Authority: WO
Inventors: 王俊超
Original assignee: 王俊超
Priority date: 2019-08-14
Filing date: 2019-08-14
Publication date: 2021-02-18

Abstract

A microburette upper lid (100, 400), a kit (101) comprising the microburette upper lid (100, 400) and a method thereof. The microburette upper lid (100, 400) is used for sealing a microburette container (200), and can be detachably connected to a mechanical moving rod (150, 550, 650). The microburette upper lid (100, 400) comprises, in structure, a lid body (110) for sealing the microburette container (200), and a receiving groove (120) which is composed of multiple apron sheets (122) and is provided above the lid body (110), and the receiving groove can be detachably connected to the mechanical moving rod (150, 550, 650).

Description

Micro tube upper cover, set containing the micro tube upper cover and method thereof

Technical field

The present invention relates to a microtube upper cover, a set containing the microtube upper cover, and a method thereof, and particularly relates to a sealing, microvolume-related experiment used in an automated biological sample preparation, analysis, and inspection system. Modules for transmission, detection and analysis.

Background technique

In order to improve the consistency of the inspection quality of medical laboratories and improve the limitations of traditional manual inspection methods, in the field of molecular medicine inspection in recent years, automated instruments and equipment have continued to introduce new ones.

The most common automated instruments for biological testing on the market use micro-porous disks for testing. Although porous disks can detect multiple samples at one time, the process of sealing and testing samples usually uses thin film for hot-press packaging, which is easy to produce In the case of incomplete packaging, the holes are not completely sealed, which may cause the risk of liquid leakage during the inspection process.

In view of this, in order to improve the defects of the automated inspection equipment and improve the inspection efficiency, an improved device and/or equipment is urgently needed in the art to improve the deficiencies of the prior art.

Summary of the invention

The content of the invention aims to provide a simplified summary of the disclosure so that readers have a basic understanding of the disclosure. This summary is not a complete summary of the disclosure, and its intention is not to point out important/key components of the embodiments of the invention or to define the scope of the invention.

In order to solve the problems existing in the prior art, the present invention provides a novel microtube upper cap, a set including the upper cap, and a method for capping and moving a biological sample in an automated biological sample detection process using the set .

One embodiment of the present invention relates to an upper cap for a microtube, which is used to seal a micrometer container and can be detachably connected with a mechanical moving rod. The upper cover of the micro tube structure includes a cover body and a receiving groove. The cover body is used to seal the micro container, and the receiving groove is composed of a plurality of skirts, and is arranged above the cover body, and the receiving groove can be detachably connected with the mechanical moving rod. In one embodiment, a part of the cap of the upper cap of the microtube is optically transparent. In an optional embodiment, the micro container refers to a micro container used for sample detection such as a micro tube or a micro disk.

Furthermore, an embodiment of the present invention relates to a kit for automatically sealing and/or moving a micro container. The set is used in automated biological sample detection equipment. It can not only cover and move trace samples and containers at the same time, achieve the purpose of simplifying tedious steps and speeding up the experimental process, avoiding the risk of leaks from incomplete sealing, and improving detection efficiency. Can reduce unnecessary waste of testing consumables. Specifically, the set includes a microtube upper cover and a mechanical moving rod, wherein the structure of the microtube upper cover is the same as that shown in any of the above embodiments. The mechanical moving rod is configured to move along the longitudinal direction, and is detachably connected with the receiving groove of the upper cover of the microtube. When the mechanical moving rod performs the sealing step, connect it through the receiving groove of the upper cap of the micro tube, and then cover the upper cap of the micro tube on the micro container, and move it through the moving mechanical moving rod if necessary. Extract and or move the micro container connected with the upper cap of the micro tube.

According to an embodiment of the present disclosure, the mechanical moving rod structurally includes an outer sleeve and an axis. The outer sleeve is detachably connected to the receiving groove, and the axis is inserted through the outer sleeve, and is configured to move along the lengthwise direction of the outer sleeve to remove the microtube connected to the outer sleeve Upper cover.

According to another embodiment of the present disclosure, the mechanical moving rod structure includes a long rod and a removal member. The long rod is detachably connected with the receiving groove; the removal member is arranged on one side of the long rod, and is configured to move along the longitudinal direction of the long rod to remove the microtube connected to the long rod cover.

According to another embodiment of the present disclosure, a method for automatically sealing and/or moving a microtube includes the following steps:

(a) Providing an automated biological sample processing and testing equipment, including a driving module and a mechanical moving rod coupled to each other, wherein the mechanical moving rod can be moved along the longitudinal direction;

(b) Provide a plurality of caps of the aforementioned microtubes to the automated biological sample processing and testing equipment;

(c) Provide a trace container to the automated biological sample processing and testing equipment, wherein the trace container contains a sample;

(d) Using the drive module to drive the mechanical moving rod to above any of the upper caps of the microtubes, and drive the mechanical moving rods to move down in the longitudinal direction and extend into the receiving grooves of the upper caps of the microtubes To grab the upper cap of the microtube;

(e) Using the drive module to drive the mechanical moving rod to move the grabbed microtube upper cover to the top of the micro container, and drive the mechanical moving rod to cover the microtube downward along the longitudinal direction On the micro-container, the micro-container is sealed to prevent the sample from overflowing, and the mechanical moving rod is used to move the micro-container as needed.

According to a specific embodiment of the present invention, in step (e), after the microtube is covered on the microcontainer with a mechanical moving rod, the drive module is directly used to move the mechanical moving rod upward along the longitudinal direction. Then the micro container can be grasped and/or moved.

According to an embodiment of the present invention, the mechanical moving rod used in the method includes an outer sleeve detachably connected with the receiving groove in structure; and a shaft center that penetrates the outer sleeve and is configured to Move up and down along the longitudinal direction of the outer sleeve, and the step further includes:

(f) Using the drive module to drive the shaft to move downward in the longitudinal direction, and remove the upper cap of the microtube from the outer tube.

According to an embodiment of the present invention, the mechanical moving rod used in the method includes a long rod detachably connected with the receiving groove in structure; and a removal member, which is arranged on one side of the long rod and is configured for use To move up and down along the longitudinal direction of the long rod, and the step further includes:

(g) Utilize the drive module to drive the removal piece to move down in the longitudinal direction to remove the upper cap of the microtube from the long rod.

After referring to the following embodiments, those skilled in the art to which the present invention belongs can easily understand the basic spirit and other purposes of the present invention, as well as the technical means and implementation methods adopted by the present invention.

Description of the drawings

In order to make the above and other objectives, features, advantages and embodiments of the present invention more comprehensible, the description of the accompanying drawings is as follows:

Figures 1A to 1C are top caps for microtubes according to an embodiment of the present invention;

2A and 2B are a set according to an embodiment of the present invention;

3A and 3B are respectively schematic diagrams of the microtubes used on the microcontainer according to different embodiments of the present invention;

4 is a schematic diagram of the upper cap of a microtube according to another embodiment of the present invention;

5A and 5B are a mechanical moving rod according to an embodiment of the present invention;

6A and 6B show a mechanical movable rod according to another embodiment of the present invention;

7A to 7G are schematic diagrams of the operation of the upper cap of the microtube of FIGS. 1A to 1C and the mechanical moving rod of FIGS. 5A and 5B according to an embodiment of the present invention; and

Fig. 8 is a schematic diagram of the operation of the upper cap of the microtube of Figs. 1A to 1C and the mechanical moving rod of Figs. 5A and 5B according to another embodiment of the present invention.

According to the usual operation method, the various features and components in the figure are not drawn to scale. The drawing method is to present the specific features and components related to the present invention in the best way. In addition, between different drawings, the same or similar component symbols are used to refer to similar components/parts.

detailed description

In order to make the description of the present disclosure more detailed and complete, the following provides an illustrative description for the implementation and specific embodiments of the present invention; but this is not the only way to implement or use the specific embodiments of the present invention. The implementation manners cover the characteristics of a number of specific embodiments and the method steps and sequences used to construct and operate these specific embodiments. However, other specific embodiments can also be used to achieve the same or equal functions and sequence of steps.

Although the numerical ranges and parameters used to define the wider range of the present invention are approximate numerical values, the relevant numerical values in the specific embodiments are presented here as accurately as possible. However, any value inherently inevitably contains the standard deviation due to individual test methods. Here, "about" usually means that the actual value is within plus or minus 10%, 5%, 1%, or 0.5% of a specific value or range. Or, the word "about" means that the actual value falls within the acceptable standard error of the average value, depending on the consideration of a person with ordinary knowledge in the technical field of the present invention. Except for the experimental examples, or unless otherwise clearly stated, all ranges, quantities, values and percentages used herein (for example, used to describe the amount of material, length of time, temperature, operating conditions, quantity ratio and other similar Those) have been modified by "about". Therefore, unless otherwise stated to the contrary, the numerical parameters disclosed in this specification and the accompanying patent scope are approximate values and can be changed according to requirements. At least these numerical parameters should be understood as the indicated effective number of digits and the value obtained by applying the general carry method.

The term "biological sample" refers to a biological sample suitable for processing by the kit and/or biological sample processing and testing equipment of the present invention, for example, a biological sample for testing or nucleic acid extraction, such as blood , Body fluids, animal tissues, plant tissues, viruses, free nucleic acids, and eukaryotic and/or prokaryotic cells.

In this article, the term "automated biological sample processing and testing equipment" refers to automated equipment on the market for processing biological samples, for example, automated nucleic acid extraction equipment and automated biological sample testing (eg, biochemical, blood, immune, molecular testing, etc.) Equipment etc. In one embodiment, the automated biological sample processing and detection equipment refers to an automated polymerase chain reaction equipment or a real-time polymerase chain reaction equipment.

In this article, the term "mechanical moving rod" refers to the mechanized arm module mounted on the conventional and well-known automated biological sample processing and testing equipment. The mechanized arm usually moves in two-dimensional and/or three-dimensional directions in a linear manner for grasping And move the sample to be tested and/or the items needed for the test (for example, reagents or consumables), etc.

Unless otherwise defined in this specification, the scientific and technical terms used herein have the same meaning as understood and used by those with ordinary knowledge in the technical field of the present invention. In addition, without conflict with context, the singular nouns used in this specification cover the plural nouns; and the plural nouns also cover the singular nouns.

It is known that the common automated biological sample processing and testing equipment on the market, especially the systems applied in the field of molecular testing, usually use micro-porous disks (such as 24, 48, 96, 384 or 1536 well disks) or multi-well testing consumables. Continuous microtubes have limitations in the application of automated inspections. For example, the conventionally well-known sealed microporous disk is covered by hot-pressing film, but this method is not easy to seal, and it is difficult to make every hole on the porous disk completely sealed. The heat vapor escapes after heating, resulting in a short reaction sample volume. , Causing the risk of misjudgment of the experimental result reading data, or the potential risk of cross-contamination due to liquid leakage between different holes. In addition, the process of detecting biological samples in the prior art is likely to cause unnecessary waste of resources; for example, the micro-porous disk is a disposable consumable. When the number of samples to be tested is low, even if there are multiple unused holes, Once used, the micro-porous disk must be discarded directly, resulting in a waste of resources.

In order to solve the aforementioned problems in the prior art, the inventor of the present case proposes a novel upper cap for microtubes, which can be covered on any hole of a single microtube, multiple rows of microtubes or a microporous disc to seal biological cells. The test sample is placed in the hole of the micro tube or micro multi-well plate, which is convenient for processing and/or detecting biological samples. Please refer to FIGS. 1A to 1C, which are schematic diagrams of the upper cap 100 for the microtube according to an embodiment of the present invention. Please refer to the drawings. The upper cap 100 for the microtube of the present invention structurally includes a cap 110 for sealing the micro container. The cap 110 is mainly composed of a top plate 112 and a ring 114, wherein the ring 114 is provided with Below the top plate 112, it is used to be inserted into the hole of the microtube or the microporous disk, adjacent to the tube wall of the microtube or the hole, and the biological sample to be tested is sealed in the hole of the microtube or the microporous disk through this structure. A receiving groove 120 is provided above the top plate 112 of the cover 110, which is composed of a plurality of skirt pieces 122. The lower end of the mechanical moving rod (not shown in the figure) can be extended into the receiving groove 120, and a micro tube The upper cover 100 is tightly integrated. In an optional embodiment, the plurality of skirt pieces 122 are at least two, three, four, five, six, seven, or eight. Furthermore, referring to Figures 1B and 1C, the top plate 112 of the microtube upper cover 100 of the present invention is transparent relative to the top of the microtube or the hole. Various light sources with wavelengths of 300-800nm can be introduced through the top plate 112 to excite the tube. The fluorescent substance contained in the biological sample can at the same time derive emission fluorescence through the top plate 112 for optical detection. Those with ordinary knowledge in the technical field to which the present invention pertains should be able to understand the choice of wavelength for the material to be detected to determine the wavelength to be introduced.

2A and 2B are schematic diagrams of the set 101 of the present invention according to an embodiment of the present invention. The set 101 includes the microtube upper cover 100 and the mechanical moving rod 150 shown in FIG. 1. The mechanical moving rod 150 includes a rod-shaped member 152 and an actuator 154, wherein the actuator 154 is used to drive the rod-shaped member 152 to move along the longitudinal direction, that is, the lower end of the rod-shaped member 152 can extend downward or Move up. It should be noted that the mechanical moving rod 150 described herein is a robotic arm in an automated biological sample processing and testing device, which is used to grab and/or move samples, reagents, and other components. Therefore, the mechanical moving rod 150 is configured to be able to move in an automated biological sample processing and testing device. In an embodiment of the present invention, please refer to FIGS. 1 and 2 at the same time. The present invention utilizes the lower end of the rod-shaped member 152 of the mechanically movable rod 150 in the set 101 to extend into the receiving groove 120 of the upper cap 100 of the microtube Among them, there is a gap between the skirt pieces 122 on the receiving groove 120, so that the upper end of the formed receiving groove 120 is elastic, so that the rod-shaped member 152 can open each skirt piece 122 through the upper end of the receiving groove 120 to make the lower end A part of it enters the receiving groove 120 so that the upper cap 100 of the microtube is sleeved and clamped on the rod 152. The mechanical moving rod 150 shown can take advantage of the characteristics of the upper cap 100 of the microtube of the present invention to grab the upper cap and move it to a specific position.

In addition, in a non-limiting embodiment, a person with ordinary knowledge in the technical field of the present invention can configure an appropriate number of mechanical moving rods into the automated biological sample processing and testing equipment according to actual use requirements. For example, a single automated biological sample processing and testing device can be equipped with one, two, three or four mechanical moving rods. Furthermore, the position of the mechanical moving rod in the automated biological sample processing and testing equipment is also determined according to actual usage requirements.

3A and 3B show the applicable components of the microtube upper cap 100 of the present invention according to different embodiments. Please refer to FIG. 3A first, the microtube upper cap 100 can be closed on the microtube 200. Furthermore, referring to FIG. 3B, there are a plurality of microtube upper caps 100 which are respectively covered on the holes of the microporous disk 296.

4 is a microtube upper cover 400 according to an embodiment of the present invention. As shown in the figure, the microtube upper cover 400 is a structure composed of four microtube upper covers 100. Therefore, the microtube upper cover 400 It can be simultaneously covered on four microtubes or four holes in a microporous disk. The microtube upper cover 400 is structurally connected to each other with four microtube upper covers 100, and a hole 105 is formed in the middle, which can also be used for the mechanical moving rod to pass through and engage with it, so that the mechanical moving rod can be It is detachably connected with the upper cap of the micro tube.

5A and 5B show a mechanical moving rod 550 according to an embodiment of the present invention. In automated biological sample processing and testing equipment (such as automated PCR equipment), those with ordinary knowledge in the technical field of the present invention should be able to understand the mechanical configuration of the robotic arm in the equipment, which will not be repeated here. In this embodiment, the robotic arm of the present invention is presented in the form of a mechanically movable rod 550, and is characterized by including an actuator 554 and a rod-shaped member 558 coupled to each other. In order to remove the upper cap of the microtube from the mechanically movable rod, the rod-shaped member 558 is structurally provided with an outer tube 552 and a shaft 556, and each member is configured to enable the outer tube 552 through an actuator 554. And/or the axis 556 moves along the longitudinal direction. Specifically, as shown in FIG. 2B, the outer sleeve 552 can be inserted into the receiving groove of the upper cap of the microtube, so that the upper cap of the microtube can be engaged with the lower end of the outer sleeve 552. The shaft 556 passes through the outer tube 552 and is configured to be driven by the actuator 554 to move the shaft 556 along the longitudinal direction of the outer tube 552. Referring to Figure 5B, when the shaft 556 extends downward and moves to protrude beyond the outer sleeve 552, the upper cap of the microtube that is engaged with the lower part of the outer sleeve 552 can be pushed down to remove the microtube from the outer sleeve 552. Cover the tube (not shown in the figure). In another embodiment, the outer tube 552 and the shaft center 556 can also be configured to engage with the upper cap of the microtube through the shaft center 556, and the diameter of the outer tube 552 is larger than the diameter of the microtube receiving groove, so that the actuator When the lower end of the 554 drive shaft 556 moves upward along the lengthwise direction of the outer tube 552, the lower end of the shaft 556 will be folded into the outer tube 552, and at the same time, the upper cap of the microtube can be resisted by the outer tube 552 to make the shaft center 556 remove the cap of the microtube.

6A and 6B show a mechanical moving rod 650 according to another embodiment of the present invention. The mechanical moving rod 650 includes an actuator 654, a long rod 652, and a removal member 656 coupled to each other, wherein the long rod 652 and/or the removal member 656 are driven by the actuator 654 to move along the longitudinal direction Move in the direction to extend the lower end of the long rod 652 into the receiving groove of the upper cap of the microtube, so that the upper cap of the microtube is engaged with the lower end of the long rod 652. The removing member 656 is arranged around the long rod 652 and is coupled with the actuator 654. In this embodiment, the removal member 656 is an annular member. Referring to FIG. 6B, in order to remove the upper cap of the micro tube at the lower end of the long rod 652, the removal member 656 can be driven to move downward by the actuator 654 to push out the micro tube that is clamped at the lower end of the long rod 652. In another embodiment, the removal member 656 does not move, and the actuator 654 drives the long rod 652 to move upward, shortening the distance between the lower end of the long rod 652 and the removal member 656, and the removal member 656 is sleeved on the long rod 652. Remove the upper cap of the lower microtube. The method of removing the upper cap of the microtube is only an example. Without departing from the spirit of the present invention, a person with ordinary knowledge in the art can use his usual experience to modify the removal member 656 relative to the long rod 652. Position to remove the upper cap of the microtube on the mechanical moving rod 650. In an optional embodiment, the driving of each member in the mechanical moving rod can be configured through the configuration of mechanical members, for example, wheels, gears, electromagnetic modules, etc.

7A to 7G show a method for automatically sealing and/or moving a micro container according to an embodiment of the present invention. It should be noted that the components proposed in the present invention are applied to automated biological sample processing and testing equipment. It is generally known that the equipment is usually provided with a drive module, a calculation module, and a detection module that are electrically coupled to each other, so it will not be described in detail here. The feature of the present invention is to provide a novel upper cap of a microtube, and an automated arm (ie, a mechanical moving rod) suitable for automated biological sample processing and testing equipment, and is different from the prior art in that the technical feature is that only a single automated The arm can perform the steps of covering and moving at the same time, which simplifies the automated instrument processing flow, and the method proposed by the present invention can also avoid resource waste and effectively use each consumable.

Specifically, please refer to FIG. 7A. In this embodiment, the mechanical moving rod 550 shown in FIGS. 5A and 5B and the microtube upper cap 100 shown in FIGS. 1A to 1C are taken as examples.

First of all, usually during the inspection process, it is necessary to provide a micro container containing the biological sample to the automated biological sample processing and testing equipment (not shown in the figure). In this embodiment, the micro container is presented in the form of a micro tube 200 and placed on the micro tube rack 202.

The steps of this set are executed as follows:

First, seal biological samples

The mechanical moving rod 550 drives the rod-shaped member 558 through the actuator 554 to move in the longitudinal direction and extend into the receiving groove of the upper cap 100 of the microtube (please refer to FIGS. 5A and 5B at the same time, and FIGS. 1A to 1C) ), the upper cap 100 of the microtube is clamped to the lower end of the rod-shaped member 558, and then the rod-shaped member 558 is moved upward in the longitudinal direction through the actuator 554, and the lower end of the rod-shaped member 558 is moved upward to extract the microtube The upper cover 100, and then move the mechanical moving rod 550 to the top of the target microtube 200, the lower end of the rod 558 is extended downward through the actuator 554, and the microtube upper cover 100 is clamped on the microtube 200, so that It is tightly combined and seals the biological sample.

Second, move the microtube 200

After capping the upper cap 100 of the microtube, please refer to FIGS. 7C and 7D at the same time. The mechanical moving rod 550 of the present invention directly moves the lower end of the rod-shaped member 558 upward along the longitudinal direction through the actuator 554, and extracts and The micro-container 200 is tightly coupled with the upper cap 100 of the tube, and the micro-container 200 is moved to another micro-tube rack 202' through a moving mechanical moving rod 550. It can be seen that one of the advantages of the present invention is that a single automated arm (ie, a mechanical moving rod) can simultaneously perform the steps of covering and moving, which can simplify tedious steps and mechanical settings, make the automated processing process more efficient, and can Reduce the development cost of automated instruments.

Third, remove the upper cap of the micro tube 100

Please refer to FIGS. 7E to 7G at the same time, the mechanical moving rod 550 drives the shaft center 556 of the rod-shaped member 558 through the actuator 554 to extend out of the outer sleeve 552 and engage with the lower end of the rod-shaped member 558 (Ie, the lower end of the outer tube 552) the upper cap 100 of the microtube is removed to separate it from the outer tube 552, and is finally left on the microtube rack 202' for subsequent detection steps.

FIG. 8 is an embodiment in which a set containing a mechanically movable rod 550 and a microtube upper cap 100 is used in a microporous disk 296 according to an embodiment of the present invention.

The method of removing the cap and the cap of the mechanical moving rod 550 and the upper cap 100 of the microtube of the present invention is the same as described above, and will not be repeated here. The only difference here is that the micro container is a micro porous disc 296. The micro porous plate 296 has a disc-shaped structure and is provided with a plurality of micro holes for loading the biological sample. The mechanical moving rod 550 of the present invention can also directly cover the microtube upper cover 100 on the micropores of the microporous disk 296 and tightly integrate with it. Furthermore, the actuator 554 of the mechanically moving rod 550 can also be used to drive the rod-shaped member 558 to move in the longitudinal direction, so that the lower end of the rod-shaped member 558 moves upward, and directly connects the microtube tightly combined with the microtube upper cap 100 The porous disk 296 is extracted, and the micro porous disk 296 can be moved to a specific position as needed.

The foregoing embodiments are exemplified by a single mechanical moving rod. However, those skilled in the art should understand that the automated biological sample processing and testing equipment can be equipped with at least one mechanical moving rod, such as: 1. Two, three, four or five or more moving rods implement the method of the present invention. For example, if you take a microtube as an example, two mechanical moving rods can increase the speed of the cap and move the microtube; if you take the microporous disk as an example, the same can increase the speed of the cap and move the microtube. In the case of a porous disk, the micro porous disk can be extracted in a multi-point combination. For example, use two side-by-side mechanical moving rods to engage with any two microtube upper caps near the center of the microporous disk, or two mechanical moving rods can also be aligned with any diagonal line on the microporous disk. The caps of the two microtubes are snapped together.

Although the specific embodiments of the present invention are disclosed in the above embodiments, they are not intended to limit the present invention. Those with ordinary knowledge in the technical field to which the present invention belongs will not deviate from the principles and spirit of the present invention. Various changes and modifications can be made to it, so the protection scope of the present invention should be defined by the accompanying patent application.

【Symbol Description】

The main component symbols are listed as follows:

100 Micro tube top cover

101 Sets

105 Hole

110 Cover body

112 Roof

114 Rings

120 Receiving slot

122 Skirts

150, 550, 650 Mechanical moving rod

152 Rods

154 Actuator

200 Micro tube

202 Micro tube rack

296 Micro porous disc

400 Micro tube top cover

552 Outer tube

554, 654 Actuator

556 Axis

558 Rods

652 Long pole

656 Removal parts.

Claims

An upper cap for a microtube is used to seal a microcontainer and can be detachably connected with a mechanical moving rod, comprising:

A lid for sealing the micro container; and

A receiving groove is composed of a plurality of skirt pieces, is arranged above the cover body, and is detachably connected with the mechanical moving rod.
The microtube upper cap according to claim 1, wherein a part of the cap body is optically transparent.
A kit used to automatically seal and/or move a micro container, including:

A cap on a micro tube, including:

A lid for tightly sealing the micro container; and

A receiving groove, composed of a plurality of skirt pieces, arranged above the cover; and

A mechanically movable rod, wherein the mechanically movable rod is configured to be movable along the longitudinal direction, and is detachably connected to the receiving groove, and wherein the mechanically movable rod is used for performing the sealing step When connecting with the receiving groove of the upper cap of the micro tube, the upper cap of the micro tube is closed on the micro container, and if necessary, the capped micro tube is moved through the mechanical moving rod. The capped micro container.
The kit of claim 3, wherein a part of the cap body of the microtube upper cap is optically transparent.
The set of claim 3, wherein the mechanical moving rod comprises:

An outer sleeve detachably connected with the receiving groove; and

A shaft is penetrated in the outer tube, and is configured to move up and down along the longitudinal direction of the outer tube to remove the upper cap of the microtube connected to the outer tube.
The set of claim 3, wherein the mechanical moving rod comprises:

A long rod detachably connected with the receiving groove; and

A removal piece, arranged on one side of the long rod, configured to move up and down along the lengthwise direction of the long rod, for removing the upper cap of the micro tube connected to the long rod .
An automated method for sealing and/or moving a microtube includes the following steps:

(a) Provide an automated biological sample processing and testing equipment, including a driving module and a mechanical moving rod coupled to each other, wherein the mechanical moving rod can be moved along the longitudinal direction;

(b) Providing a plurality of upper caps of the microtubes according to claim 1 into the automated biological sample processing and testing equipment;

(c) providing a micro container to the automated biological sample processing and testing equipment, wherein the micro container contains a sample;

(d) Using the drive module to drive the mechanical moving rod to the top of any of the upper caps of the microtubes, and drive the mechanical moving rods to move downward along the longitudinal direction and extend into the microtubes In the receiving groove of the cover to grab the upper cover of the microtube; and

(e) Using the drive module to drive the mechanical moving rod to move the grabbed upper cap of the micro tube to the top of the micro container, and drive the mechanical moving rod to move the rod downward along the longitudinal direction The upper cap of the micro tube is covered on the micro container, the micro container is sealed to prevent the sample from overflowing, and the mechanical moving rod is used to move the micro container as needed.
The method according to claim 7, wherein in the step (e), after the upper cap of the micro tube is covered on the micro container by the mechanical moving rod, the driving module is directly used to make the The mechanical moving rod moves upward along the longitudinal direction to grasp and/or move the micro container.
The method according to claim 7, wherein the mechanically movable rod comprises: an outer tube detachably connected with the receiving groove; and a shaft center, which penetrates the outer tube and is configured for use To move up and down along the longitudinal direction of the outer sleeve, and wherein the step further includes:

(f) Using the drive module to drive the shaft to move downward in the longitudinal direction, and remove the upper cap of the microtube from the outer tube.
The method of claim 7, wherein the mechanically movable rod comprises: a long rod detachably connected with the receiving groove; and a removal member, which is arranged on one side of the long rod and is configured For moving up and down along the longitudinal direction of the long rod, and the step further includes:

(g) Utilizing the driving module to drive the removal member to move downward in the longitudinal direction, and remove the upper cap of the micro tube from the long rod.