TW202002785A - Bacterial blight disease control method - Google Patents

Abstract

The present invention aims to provide a bacterial spot eradication method that can achieve a high eradication effect even without using a chemical agent. This bacterial spot eradication method applies nanobubble water to plants.

Description

Prevention and treatment of speckled bacterial disease

The invention relates to a method for preventing and controlling spotted bacterial disease.

Most of plant diseases are infectious diseases that cause pathogenic bacteria inhabiting soil, seeds, plants and weeds. In particular, it is difficult to sterilize the pathogenic bacteria of soil habitat with chemicals, so soil bacterial diseases (soil infectious diseases) such as spot bacterial disease and bacterial wilt disease are well known as refractory diseases. In addition, regarding the main chemical agents used in soil sterilization (such as fumigants), the selectivity of the target organism is low, and in addition to pathogenic bacteria, it also acts on a wide range of organisms, so it is worried about The biological effects of the human body or the surrounding environment.

In view of this situation and concerns, for example, Patent Document 1 describes "A law for preventing and controlling soil infectious diseases accompanying the growth of crops, which is characterized by the resistance of useful microorganisms to plant diseases during the sowing and seedling stages of crops. Derivatives are added to cultivated soils or crops and soil pH correction substances are given to cultivated soils, thereby deriving the growth promotion effect of crops after planting on the farmland and the effect of suppressing the incidence of soil infectious diseases." ([Request 1]). [Prior Art Literature] [Patent Literature]

[Patent Document 1] Japanese Patent Laid-Open No. 2015-061826

[Problems to be solved by the invention]

Therefore, an object of the present invention is to provide a method for preventing and controlling spotted bacterial diseases that can achieve a high control effect even without using chemical agents. [Means to solve the problem]

The inventors made intensive studies to achieve the above-mentioned problems, and as a result, it was found that by applying nano bubble water to plants, the prevention effect of spotted bacterial disease becomes high even without using chemicals, and the present invention has been completed. That is, the inventor found that the above-mentioned problems can be achieved by the following configuration.

[1] A method for preventing and controlling spotted bacterial disease, in which nano bubble water is applied to plants. [2] The method for preventing and controlling spotted bacterial disease according to [1], wherein at least one of sprinkling water using the nano bubble water and supply of the culture medium diluted with the nano bubble water to the medium is implemented By. [3] The method for preventing and controlling speckle bacterial disease according to [1] or [2], wherein the mode particle diameter of the bubbles contained in the nano bubble water is 10 nm to 500 nm. [4] The method for preventing and controlling spotted bacterial disease according to any one of [1] to [3], wherein the bubbles contained in the nano bubble water include a group selected from the group consisting of oxygen, nitrogen, carbon dioxide, and ozone At least one gas in the group. [5] The method for preventing and controlling spotted bacterial disease according to any one of [1] to [4], wherein the nano bubble water has bubbles of 1×10 ⁸ cells/mL to 1×10 ¹⁰ cells/mL . [6] The method for preventing and controlling spotted bacterial disease according to any one of [1] to [5], wherein the plant body is a fruit or vegetable. [7] The method for controlling spotted bacterial disease as described in [6], wherein the plant body is tomato. [Effect of invention]

According to the present invention, it is possible to provide a method for preventing and controlling spotted bacterial diseases that can achieve a high control effect even without using chemical agents.

Hereinafter, the present invention will be described in detail. The description of the constituent elements described below may be based on the representative embodiments of the present invention, but the present invention is not limited to such embodiments. In addition, in the specification of the present application, the numerical range indicated by "~" refers to a range including the numerical values described before and after "~" as the lower limit value and the upper limit value.

The method for preventing and controlling spotted bacterial disease of the present invention (hereinafter also simply referred to as "the control method for the present invention") is a method for preventing and controlling spotted bacterial disease by applying nano bubble water to plants. Here, the "spotted bacterial disease" is a kind of soil bacterial disease (soil infectious disease) as mentioned above. As a symptom thereof, it is known that water-fed polygons appear on leaves, petioles, or buds of plants Symptoms of lesions. Specific examples of spot bacterial disease include: alfalfa spot bacterial disease (Xanthomonas alfalfae pv. alfalfae); cannabis spot bacterial disease (Xanthomonas campestris pv. cannabis); carrot spot bacterial disease (Xanthomonas hortorum pv. carotae); ivy Xanthomonas hortorum pv. hederae; Xanthomonas campestris pv. papavericola; Xanthomonas axonopodis pv. physalidicola; turnip, cabbage, radish and cabbage etc. Xanthomonas campestris pv. raphani); castor spot bacterial disease (Xanthomonas axonopodis pv. ricini); pepper and tomato and other spotted bacterial diseases (Xanthomonas euvesicatoria, X. vesicatoria); agave and lettuce and other spotted bacterial diseases (Xanthomonas axonopodis pv. vitians); Xanthomonas campestris pv. zinniae etc. Hereinafter, the nano bubble water and optional components used in the control method of the present invention will be described in detail.

[Nano bubble water] The nano bubble water used in the control method of the present invention is water containing bubbles with a diameter of less than 1 μm, and is water mixed with the bubbles. In addition, the "water mixed with the bubbles" refers to the water containing the bubbles inevitably contained in the water (for example, well water containing impurities) used in the generation of nano bubble water. except. Here, the diameter (particle size) of the bubbles contained in the nanobubble water and the mode diameter and the number of bubbles described later are obtained by measuring the Brownian motion speed of the bubbles in the water using the nanoparticle tracking analysis method In this specification, the value measured by the Nanoparticle Series (NanoSight Series) (manufactured by NanoSight) is used in this specification. In addition, the Nano Particle Series (NanoSight Series) (made by NanoSight) can measure the speed of Brownian motion of particles and calculate the diameter (particle Diameter), the mode diameter can be confirmed in the form of mode diameter based on the particle size distribution of the existing nanoparticles.

In the present invention, for the reason that the prevention effect of spot bacterial disease is further improved, the mode particle diameter of the bubbles contained in the nano bubble water is preferably 10 nm to 500 nm, more preferably 30 nm to 300 nm, further preferably 70 nm to 130 nm.

The gas constituting the bubbles contained in the nanobubble water is not particularly limited, and from the viewpoint of remaining in water for a long time, a gas other than hydrogen is preferred, and specific examples include air, oxygen, and nitrogen. , Fluorine, carbon dioxide and ozone. Among these, for the reason that the prevention effect of the spot bacterial disease is further improved, it is preferable to include at least one gas selected from the group consisting of oxygen, nitrogen, carbon dioxide, and ozone, in particular, regarding the growth of plants For the reason that it is good and the bubbles can remain for a longer time, it is more preferable to contain oxygen. Here, the inclusion of oxygen means that it is contained at a concentration higher than the concentration of oxygen in the air. Nitrogen and carbon dioxide are also the same. Furthermore, the concentration of oxygen is preferably 30% by volume or more in the bubbles, and preferably more than 50% by volume and 100% by volume or less.

For the reason that the prevention effect of spot bacterial disease is further improved, the nano bubble water preferably has bubbles of 1×10 ⁸ pieces/mL to 1×10 ¹⁰ pieces/mL, in particular, the generation time of bubbles The reason for the good balance with the residual properties of bubbles is more preferably bubbles having more than 1×10 ⁸ pieces/mL and less than 1×10 ¹⁰ pieces/mL, and more preferably having 5×10 ⁸ pieces/mL. ~5×10 ⁹ bubbles/mL.

The nano bubble water may also contain other components than water and bubbles. Examples of the other components include fertilizers and pesticides. The type and content of other components in nano bubble water are not particularly limited, and can be selected according to the purpose. Among them, in the present invention, it is preferable that the nano bubble water does not substantially contain radicals as the other components. Furthermore, the "substantially free of radicals" does not mean that the water used in the production of the nano bubble water (such as well water containing impurities) inevitably contains free radicals, It refers to the case where free radicals generated by a certain operation are mixed. In addition, the control method of the present invention has a high control effect even without the use of chemical agents, so the nano bubble water may not contain pesticides containing chemical agents.

Examples of the method for generating the nano bubble water include a static mixer method, a Venturi method, a cavitation method, a vapor condensation method, an ultrasonic method, a swirling reflux method, and a pressurized dissolution method And micropore method. Here, the control method of the present invention may also include a generation step of generating the nano bubble water before applying the nano bubble water. That is, the control method of the present invention may be, for example, the following control method, which includes: a generating step of taking water from a water source such as a water storage tank, a well, or agricultural water to a nano bubble generating device to generate nano bubble water; and an application step , Apply the generated nano bubble water. In addition, as a method of taking in water from the water source to the nano-bubble generating device, for example, a method of supplying water drawn from the water source to the nano-bubble generating device using a bucket or a pump, etc.; and laying it on the water source The flow path between the nano-bubble generating device is connected to the nano-bubble generating device, and the method of feeding water directly from the flow path to the nano-bubble generating device, etc.

In addition, as the method of generating the nano bubble water, a method of generating a device that does not intentionally generate free radicals is preferable. Specifically, for example, Japanese Patent Laid-Open No. 2018-15715 [0080] is used. ] Paragraphs ~ [0100] The method described in the paragraph nano bubble generation device. Furthermore, the content is incorporated into this specification.

As other nano bubble generating devices that do not intentionally generate free radicals, for example, a fine bubble generating device including: a liquid ejecting machine that ejects water; a gas mixing machine that pressurizes the gas to make It is mixed into the water ejected from the liquid ejecting machine; and a fine bubble generator generates fine bubbles in the water by passing the water mixed with the gas inside, and is characterized in that the liquid ejecting machine and all Between the fine bubble generators, the gas mixing machine pressurizes the gas and mixes it into the liquid flowing to the fine bubble generator in a pressurized state. Specifically, a method of generating using the nano bubble generating device shown in FIG. 1 can be cited. Here, the nano bubble generating device 10 shown in FIG. 1 includes a liquid ejecting machine 30, a gas mixing machine 40, and a nano bubble generating nozzle 50 inside. In addition, the liquid ejecting machine 30 includes a pump, and takes in raw water of nano bubble water (for example, well water) and ejects it. The gas mixing machine 40 has a container 41 in which compressed gas is enclosed and a substantially cylindrical gas mixing machine body 42, and while the water sprayed from the liquid ejecting machine 30 flows into the gas mixing machine body 42, the compressed gas in the container 41 The gas is introduced into the machine body 42. Thereby, water mixed with gas is generated in the gas mixing body 42. In addition, the nano-bubble generating nozzle 50 passes through the water mixed with gas and generates nano-bubbles in the water mixed with gas according to the principle of pressure dissolution. The structure of the nano bubble generating nozzle described in 2018-15715 is the same. The nano-bubble water generated in the nano-bubble generating nozzle 50 is discharged from the front end of the nano-bubble generating nozzle 50, flows out from the nano-bubble generating device 10, and passes through a flow path (not shown) to a predetermined Use place. As described above, in the nano bubble generating device 10, between the liquid ejecting machine 30 and the nano bubble generating nozzle 50, the gas mixing machine 40 mixes the compressed gas until it flows to the nano bubble generating nozzle 50 in a pressurized state. In water (raw water). With this, it is possible to avoid problems such as cavitation caused when gas is mixed into water on the suction side (suction side) of the liquid ejection machine 30. In addition, since the gas is mixed into the water in a pressurized (compressed) state, the gas can be mixed against the pressure of the water in the gas mixing part. Therefore, even if the negative pressure is not particularly generated at the gas mixing site, the gas can be mixed into the water appropriately. Furthermore, a flow path of water supplied from a water source such as a well or a water pipe is connected to the suction side of the liquid ejection machine 30, and in this flow path, the pressure of water flowing into the liquid ejection machine 30 from the upstream side of the liquid ejection machine 30 (That is, the water pressure on the suction side) may be a positive pressure. In this case, the above configuration becomes more meaningful. That is, when the water pressure (suction pressure) on the upstream side of the liquid ejection machine 30 is a positive pressure, the gas is mixed into the water on the downstream side of the liquid ejection machine 30, so the downstream side of the liquid ejection machine 30 may also be used The configuration of the nano-bubble generating device 10 in which gas is appropriately mixed into water becomes more prominent.

In addition, the water used for the production of the nano bubble water is not particularly limited, and for example, rainwater, tap water, well water, agricultural water, and distilled water can be used. Such water can also be subjected to other treatments before being supplied to the nano bubble water. Examples of other treatments include pH adjustment, precipitation, filtration, and sterilization (sterilization). Specifically, for example, when agricultural water is used, typically, agricultural water after at least one of precipitation and filtration can be used.

In the present invention, the application form of the nano-bubble water to plants differs according to the plant cultivation method, and therefore is not particularly limited. For example, in soil cultivation, the nano-bubble water is sprayed Form; in soil cultivation, the form of spraying the pesticide diluted with the nano bubble water; in nutrient solution cultivation (hydroponics, spray cultivation or solid medium cultivation) or nutrient solution soil cultivation (irrigation and fertilization cultivation) , The form of supplying the culture solution diluted with the nano-bubble water to the medium; and the form of spraying (irrigating) the nano-bubble water separately in the nutrient solution soil cultivation. Among these, for the reason that the operation is simple and the prevention effect of the spot bacterial disease is further improved, it is preferable to perform sprinkling using the nano bubble water and the culture solution diluted with the nano bubble water to the culture medium The form of at least one of the supply. In addition, the method of "sprinkling water" as one form of application is not particularly limited. When the cultivation method is soil cultivation, for example, a method of spraying water on the entire plant body, a part of the plant body (For example, stems or leaves, etc.) The method of spraying water and the method of spraying water on the soil planted with plants. In addition, when the cultivation method is nutrient liquid soil cultivation, as described above, it may be sprinkling using irrigation.

In addition, in the present invention, the application time of the nano bubble water to plants varies according to the application form and the type of plants, and therefore is not particularly limited. For example, in the case of soil cultivation of fruits and vegetables, it may be The whole period from sowing to harvest can also be applied only within a certain period (such as sowing and seedling stage).

In the present invention, the plant to which the nano bubble water is applied is not particularly limited as long as it exhibits symptoms of speckled bacterial disease, and examples thereof include Xanthomonas pescatoria and the like Plants preferred by pathogenic bacteria may preferably include fruits and vegetables. Specific examples of fruits and vegetables include solanaceous plants (eg, eggplants, eggplants, tomatoes (including small tomatoes), tree tomatoes, peppers, bell peppers, habanero peppers, green peppers, sweet peppers, and colored green peppers), five Jiake plants (such as clover, etc.), Cucurbitaceae (such as pumpkin, zucchini, cucumber, spiny melon, Vietnamese melon, bitter gourd, winter melon, chayote, loofah, gourd, watermelon, cantaloupe, melon, etc.), mallow family (Such as okra, etc.) and Rosaceae (such as strawberries, etc.). Among these, more preferred are solanaceae plants, further preferred are eggplants or tomatoes, and particularly preferred are tomatoes. [Example]

Hereinafter, the present invention will be further described in detail with examples. As long as it does not deviate from the gist of the present invention, the materials, usage amounts, ratios, processing contents, processing order, etc. shown in the following examples can be appropriately changed. Therefore, the scope of the present invention should not be limitedly interpreted by the embodiments shown below.

<Contents of the test> The experiment was conducted in the agricultural greenhouse of tomato (variety: Momotaro yoke, Takii seedlings) cultivated in Chuo-shi, Yamanashi from August to December 2017, by the following Implement differently. Test area I: Nano bubble water generated by the following method was used for the dilution of the culture solution in hydroponic cultivation of tomato (medium: rock wool). Test area II: Well water was not used for the dilution of the culture solution in hydroponic cultivation of tomato (medium: rock wool) but nano bubble water was used. Each test area was divided by adjacent agricultural greenhouses, in which 2,800 tomato plants were cultivated. In addition, although the culture medium and the amount are suitably changed according to the conventional method and according to the growth condition of the tomato, the weather, etc., they are adjusted in such a way that they become substantially the same in both test areas. In addition, before the tomato planting, for the two test areas, the chlorine-based chemicals were used to sterilize and disinfect the entire agricultural greenhouse 4 times. The amount of the chlorine-based drug per one time was performed at 300 L/10a.

<Method for generating nano bubble water> Nano bubble water is generated by the following method: using the nano bubble generation device [AQUA Solutions Division of KAKUICHI Manufacturing Co., Ltd. (now: AKA Solutions (AQUA Solutions) Co., Ltd.), 100 V, 10 L/min type] and generates bubbles (nano bubbles) in water by pressure dissolution. In addition, well water is used for generating nano-bubble water, and oxygen is used for the gas constituting the bubble (industrial oxygen, concentration: 99.5% by volume). In addition, regarding the conditions for generating the nano bubbles using the nano bubble generating device, the analysis results obtained by the nano particle analysis system NanoSight LM10 (manufactured by NanoSight) Under the following conditions. ‧Number of bubbles per 1 mL of water: 5×10 ⁸ pieces/mL ‧Mode particle size of bubbles: 100 nm

<Evaluation of the prevention and treatment effect of spotted bacterial disease> For the tomatoes cultivated in each agricultural greenhouse, visually check the stems and leaves for signs of spotted bacterial disease. The results are shown below. Test area I: No symptoms of speckled bacterial disease were observed (see Figure 2). Test area II: In mid-October, spotted bacterial disease symptoms were confirmed on some strains (see Figure 3), and in early November, spotted bacterial disease symptoms were confirmed on all strains.

10‧‧‧Nano bubble generating device 30‧‧‧Liquid ejection machine 40‧‧‧Gas mixing machine 41‧‧‧Container 42‧‧‧gas mixed into the machine body 50‧‧‧Nano bubble generating nozzle

FIG. 1 is a schematic diagram showing an example of a nano bubble generating device. FIG. 2 is an image of the stems and leaves of one tomato in the test area I. FIG. FIG. 3 is an image of the stems and leaves of one tomato in test area II.

10‧‧‧Nano bubble generating device

30‧‧‧Liquid ejection machine

40‧‧‧Gas mixing machine

41‧‧‧Container

42‧‧‧gas mixed into the machine body

50‧‧‧Nano bubble generating nozzle

Claims (7)

A method for preventing and controlling spotted bacterial disease, in which nano bubble water is applied to plants. The method for preventing and controlling spotted bacterial disease as described in item 1 of the patent application scope, wherein at least one of sprinkling water using the nano bubble water and supply of the culture medium diluted with the nano bubble water to the medium is implemented By. The method for preventing and controlling speckle bacterial disease as described in item 1 or 2 of the patent application scope, wherein the mode particle diameter of the bubbles contained in the nano bubble water is 10 nm to 500 nm. The method for preventing and controlling spotted bacterial disease as described in any one of the first to third patent claims, wherein the bubbles contained in the nanobubble water include a group selected from the group consisting of oxygen, nitrogen, carbon dioxide, and ozone At least one gas in the group. The method for preventing and controlling spotted bacterial disease as described in any one of the first to fourth patent applications, wherein the nano bubble water has bubbles of 1×10 ⁸ cells/mL to 1×10 ¹⁰ cells/mL . The method for preventing and controlling spotted bacterial disease as described in any one of claims 1 to 5, wherein the plants are fruits and vegetables. The method for preventing and controlling spotted bacterial disease as described in Item 6 of the patent application range, wherein the plant body is tomato.

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