TW201632620A - 使用前驅細胞治療視網膜變性 - Google Patents

Info

Publication number

TW201632620A

TW201632620A TW104142367A TW104142367A TW201632620A TW 201632620 A TW201632620 A TW 201632620A TW 104142367 A TW104142367 A TW 104142367A TW 104142367 A TW104142367 A TW 104142367A TW 201632620 A TW201632620 A TW 201632620A

Authority

TW

Taiwan

Prior art keywords

cells

cell

medium

derived

postpartum

Prior art date

2014-12-16

Links

• Espacenet
• Global Dossier
• Discuss

• 201000007737 Retinal degeneration Diseases 0.000 title claims abstract description 25
• 230000004258 retinal degeneration Effects 0.000 title claims abstract description 23
• 210000000130 stem cell Anatomy 0.000 title abstract description 40
• 238000011282 treatment Methods 0.000 title description 34
• 210000004027 cell Anatomy 0.000 claims abstract description 954
• 239000003636 conditioned culture medium Substances 0.000 claims abstract description 218
• 238000000034 method Methods 0.000 claims abstract description 129
• 239000000203 mixture Substances 0.000 claims abstract description 48
• 239000003795 chemical substances by application Substances 0.000 claims abstract description 31
• 210000000608 photoreceptor cell Anatomy 0.000 claims abstract description 29
• 230000001228 trophic effect Effects 0.000 claims abstract description 29
• 230000002207 retinal effect Effects 0.000 claims abstract description 22
• 210000001519 tissue Anatomy 0.000 claims description 144
• 108090000623 proteins and genes Proteins 0.000 claims description 100
• 108091008598 receptor tyrosine kinases Proteins 0.000 claims description 87
• 102000027426 receptor tyrosine kinases Human genes 0.000 claims description 85
• 241000282414 Homo sapiens Species 0.000 claims description 75
• -1 PDGF-DD Proteins 0.000 claims description 60
• 210000003954 umbilical cord Anatomy 0.000 claims description 56
• 101100208237 Bos taurus THBS2 gene Proteins 0.000 claims description 55
• 108010046722 Thrombospondin 1 Proteins 0.000 claims description 55
• 101710191666 Lactadherin Proteins 0.000 claims description 53
• 102100039648 Lactadherin Human genes 0.000 claims description 53
• 101150022345 GAS6 gene Proteins 0.000 claims description 51
• 102000004169 proteins and genes Human genes 0.000 claims description 47
• 102000004219 Brain-derived neurotrophic factor Human genes 0.000 claims description 46
• 108090000715 Brain-derived neurotrophic factor Proteins 0.000 claims description 46
• 230000014509 gene expression Effects 0.000 claims description 39
• 239000008194 pharmaceutical composition Substances 0.000 claims description 36
• 210000004369 blood Anatomy 0.000 claims description 30
• 239000008280 blood Substances 0.000 claims description 30
• 238000004519 manufacturing process Methods 0.000 claims description 23
• 208000002780 macular degeneration Diseases 0.000 claims description 21
• 102000004890 Interleukin-8 Human genes 0.000 claims description 19
• 108090001007 Interleukin-8 Proteins 0.000 claims description 19
• 229940096397 interleukin-8 Drugs 0.000 claims description 19
• XKTZWUACRZHVAN-VADRZIEHSA-N interleukin-8 Chemical compound C([C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@@H](NC(C)=O)CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCSC)C(=O)N1[C@H](CCC1)C(=O)N1[C@H](CCC1)C(=O)N[C@@H](C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC=1C=CC(O)=CC=1)C(=O)N[C@H](CO)C(=O)N1[C@H](CCC1)C(N)=O)C1=CC=CC=C1 XKTZWUACRZHVAN-VADRZIEHSA-N 0.000 claims description 19
• 108090000742 Neurotrophin 3 Proteins 0.000 claims description 17
• 206010064930 age-related macular degeneration Diseases 0.000 claims description 16
• 230000006727 cell loss Effects 0.000 claims description 16
• 210000002901 mesenchymal stem cell Anatomy 0.000 claims description 15
• 108010017992 platelet-derived growth factor C Proteins 0.000 claims description 15
• 101001008874 Homo sapiens Mast/stem cell growth factor receptor Kit Proteins 0.000 claims description 13
• 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 claims description 13
• 102100027754 Mast/stem cell growth factor receptor Kit Human genes 0.000 claims description 13
• 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 claims description 13
• 210000002950 fibroblast Anatomy 0.000 claims description 13
• 102100022464 5'-nucleotidase Human genes 0.000 claims description 11
• 102100022749 Aminopeptidase N Human genes 0.000 claims description 11
• 102100032912 CD44 antigen Human genes 0.000 claims description 11
• 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 claims description 11
• 101000678236 Homo sapiens 5'-nucleotidase Proteins 0.000 claims description 11
• 101000757160 Homo sapiens Aminopeptidase N Proteins 0.000 claims description 11
• 101000868273 Homo sapiens CD44 antigen Proteins 0.000 claims description 11
• 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 claims description 11
• 101000800116 Homo sapiens Thy-1 membrane glycoprotein Proteins 0.000 claims description 11
• 108090000028 Neprilysin Proteins 0.000 claims description 11
• 102000003729 Neprilysin Human genes 0.000 claims description 11
• 102100024616 Platelet endothelial cell adhesion molecule Human genes 0.000 claims description 11
• 102100033523 Thy-1 membrane glycoprotein Human genes 0.000 claims description 11
• 210000002798 bone marrow cell Anatomy 0.000 claims description 11
• 210000005260 human cell Anatomy 0.000 claims description 11
• 230000004792 oxidative damage Effects 0.000 claims description 11
• 102100028972 HLA class I histocompatibility antigen, A alpha chain Human genes 0.000 claims description 10
• 108010075704 HLA-A Antigens Proteins 0.000 claims description 10
• 108010058597 HLA-DR Antigens Proteins 0.000 claims description 10
• 102000006354 HLA-DR Antigens Human genes 0.000 claims description 10
• 101001076408 Homo sapiens Interleukin-6 Proteins 0.000 claims description 9
• 101000763314 Homo sapiens Thrombomodulin Proteins 0.000 claims description 9
• 102100026966 Thrombomodulin Human genes 0.000 claims description 9
• 239000003937 drug carrier Substances 0.000 claims description 9
• 239000000126 substance Substances 0.000 claims description 9
• 210000002472 endoplasmic reticulum Anatomy 0.000 claims description 8
• 239000000790 retinal pigment Substances 0.000 claims description 8
• 101000898034 Homo sapiens Hepatocyte growth factor Proteins 0.000 claims description 7
• 102000034615 Glial cell line-derived neurotrophic factor Human genes 0.000 claims description 5
• 108091010837 Glial cell line-derived neurotrophic factor Proteins 0.000 claims description 5
• 208000011325 dry age related macular degeneration Diseases 0.000 claims description 4
• 101000868152 Homo sapiens Son of sevenless homolog 1 Proteins 0.000 claims 4
• 102100028976 HLA class I histocompatibility antigen, B alpha chain Human genes 0.000 claims 2
• 102100028971 HLA class I histocompatibility antigen, C alpha chain Human genes 0.000 claims 2
• 108010058607 HLA-B Antigens Proteins 0.000 claims 2
• 108010052199 HLA-C Antigens Proteins 0.000 claims 2
• 108010010378 HLA-DP Antigens Proteins 0.000 claims 2
• 102000015789 HLA-DP Antigens Human genes 0.000 claims 2
• 108010062347 HLA-DQ Antigens Proteins 0.000 claims 2
• 230000004064 dysfunction Effects 0.000 claims 1
• 230000006907 apoptotic process Effects 0.000 abstract description 9
• 210000003583 retinal pigment epithelium Anatomy 0.000 description 251
• 239000002609 medium Substances 0.000 description 202
• 206010057249 Phagocytosis Diseases 0.000 description 127
• 230000008782 phagocytosis Effects 0.000 description 127
• 239000003446 ligand Substances 0.000 description 85
• 239000002243 precursor Substances 0.000 description 63
• 108091003079 Bovine Serum Albumin Proteins 0.000 description 59
• 239000012091 fetal bovine serum Substances 0.000 description 59
• 230000000694 effects Effects 0.000 description 55
• 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 54
• 238000003556 assay Methods 0.000 description 54
• 239000001963 growth medium Substances 0.000 description 51
• 102100036034 Thrombospondin-1 Human genes 0.000 description 50
• 210000001508 eye Anatomy 0.000 description 48
• 102000003745 Hepatocyte Growth Factor Human genes 0.000 description 46
• 108090000100 Hepatocyte Growth Factor Proteins 0.000 description 46
• 235000018102 proteins Nutrition 0.000 description 44
• 230000000242 pagocytic effect Effects 0.000 description 43
• 229940077737 brain-derived neurotrophic factor Drugs 0.000 description 42
• 239000000523 sample Substances 0.000 description 36
• 210000002744 extracellular matrix Anatomy 0.000 description 32
• 238000004113 cell culture Methods 0.000 description 30
• 230000004069 differentiation Effects 0.000 description 28
• 239000000047 product Substances 0.000 description 28
• 230000003412 degenerative effect Effects 0.000 description 27
• 238000000338 in vitro Methods 0.000 description 27
• 102100033762 Proheparin-binding EGF-like growth factor Human genes 0.000 description 26
• 210000002826 placenta Anatomy 0.000 description 26
• 210000005059 placental tissue Anatomy 0.000 description 26
• 238000005516 engineering process Methods 0.000 description 25
• HNXQXTQTPAJEJL-UHFFFAOYSA-N 2-aminopteridin-4-ol Chemical compound C1=CN=C2NC(N)=NC(=O)C2=N1 HNXQXTQTPAJEJL-UHFFFAOYSA-N 0.000 description 24
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 24
• 101000871708 Homo sapiens Proheparin-binding EGF-like growth factor Proteins 0.000 description 24
• WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 24
• 108010038512 Platelet-Derived Growth Factor Proteins 0.000 description 24
• 102000010780 Platelet-Derived Growth Factor Human genes 0.000 description 24
• 239000008103 glucose Substances 0.000 description 24
• 102000004889 Interleukin-6 Human genes 0.000 description 23
• 108020004459 Small interfering RNA Proteins 0.000 description 22
• 238000001514 detection method Methods 0.000 description 22
• 230000012010 growth Effects 0.000 description 22
• 238000002360 preparation method Methods 0.000 description 22
• 108020003175 receptors Proteins 0.000 description 22
• 239000004055 small Interfering RNA Substances 0.000 description 22
• UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 22
• 239000003102 growth factor Substances 0.000 description 20
• 102000005962 receptors Human genes 0.000 description 20
• 102000004190 Enzymes Human genes 0.000 description 19
• 108090000790 Enzymes Proteins 0.000 description 19
• 108090001005 Interleukin-6 Proteins 0.000 description 19
• LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 19
• 229940088598 enzyme Drugs 0.000 description 19
• 230000001537 neural effect Effects 0.000 description 19
• 239000002953 phosphate buffered saline Substances 0.000 description 19
• 108091008695 photoreceptors Proteins 0.000 description 19
• 239000000243 solution Substances 0.000 description 19
• 238000002965 ELISA Methods 0.000 description 18
• 238000008157 ELISA kit Methods 0.000 description 18
• 210000002966 serum Anatomy 0.000 description 18
• 230000001573 trophoblastic effect Effects 0.000 description 18
• 241000700159 Rattus Species 0.000 description 17
• 108010007093 dispase Proteins 0.000 description 17
• 238000002474 experimental method Methods 0.000 description 17
• 108060005980 Collagenase Proteins 0.000 description 16
• 102000029816 Collagenase Human genes 0.000 description 16
• 229960002424 collagenase Drugs 0.000 description 16
• 230000035945 sensitivity Effects 0.000 description 16
• 101000931422 Photobacterium phosphoreum Methenyltetrahydrofolate cyclohydrolase Proteins 0.000 description 15
• 101000752546 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) Transaminated amino acid decarboxylase Proteins 0.000 description 15
• 108010031318 Vitronectin Proteins 0.000 description 15
• 102100035140 Vitronectin Human genes 0.000 description 15
• 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 15
• 239000000463 material Substances 0.000 description 15
• 108010035532 Collagen Proteins 0.000 description 14
• 102000008186 Collagen Human genes 0.000 description 14
• 102000018233 Fibroblast Growth Factor Human genes 0.000 description 14
• 108050007372 Fibroblast Growth Factor Proteins 0.000 description 14
• JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 14
• 102000036693 Thrombopoietin Human genes 0.000 description 14
• 108010041111 Thrombopoietin Proteins 0.000 description 14
• 229920001436 collagen Polymers 0.000 description 14
• 229940126864 fibroblast growth factor Drugs 0.000 description 14
• 238000010186 staining Methods 0.000 description 14
• 102000004230 Neurotrophin 3 Human genes 0.000 description 13
• 150000001875 compounds Chemical class 0.000 description 13
• 230000006378 damage Effects 0.000 description 13
• 229960002989 glutamic acid Drugs 0.000 description 13
• 229940032018 neurotrophin 3 Drugs 0.000 description 13
• 230000002829 reductive effect Effects 0.000 description 13
• 238000012360 testing method Methods 0.000 description 13
• 239000013598 vector Substances 0.000 description 13
• 108010055166 Chemokine CCL5 Proteins 0.000 description 12
• 102000003972 Fibroblast growth factor 7 Human genes 0.000 description 12
• 108090000385 Fibroblast growth factor 7 Proteins 0.000 description 12
• 229930182555 Penicillin Natural products 0.000 description 12
• 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 12
• 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 12
• 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 12
• 239000003963 antioxidant agent Substances 0.000 description 12
• 235000006708 antioxidants Nutrition 0.000 description 12
• 201000010099 disease Diseases 0.000 description 12
• 108010044426 integrins Proteins 0.000 description 12
• 102000006495 integrins Human genes 0.000 description 12
• 230000036542 oxidative stress Effects 0.000 description 12
• 229940049954 penicillin Drugs 0.000 description 12
• 229920000747 poly(lactic acid) Polymers 0.000 description 12
• 238000000926 separation method Methods 0.000 description 12
• 239000012103 Alexa Fluor 488 Substances 0.000 description 11
• 102100034608 Angiopoietin-2 Human genes 0.000 description 11
• 102100032367 C-C motif chemokine 5 Human genes 0.000 description 11
• 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 11
• 101000669513 Homo sapiens Metalloproteinase inhibitor 1 Proteins 0.000 description 11
• 102100039364 Metalloproteinase inhibitor 1 Human genes 0.000 description 11
• 108010017842 Telomerase Proteins 0.000 description 11
• 102000046299 Transforming Growth Factor beta1 Human genes 0.000 description 11
• 230000003399 chemotactic effect Effects 0.000 description 11
• 238000001727 in vivo Methods 0.000 description 11
• 238000002955 isolation Methods 0.000 description 11
• 239000007758 minimum essential medium Substances 0.000 description 11
• 238000010899 nucleation Methods 0.000 description 11
• 230000008569 process Effects 0.000 description 11
• 230000028327 secretion Effects 0.000 description 11
• 229960005322 streptomycin Drugs 0.000 description 11
• 108010048036 Angiopoietin-2 Proteins 0.000 description 10
• 241000699666 Mus <mouse, genus> Species 0.000 description 10
• 238000003559 RNA-seq method Methods 0.000 description 10
• 239000013592 cell lysate Substances 0.000 description 10
• 239000003814 drug Substances 0.000 description 10
• 238000009472 formulation Methods 0.000 description 10
• RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 10
• 239000000049 pigment Substances 0.000 description 10
• 239000007787 solid Substances 0.000 description 10
• 239000006228 supernatant Substances 0.000 description 10
• 108020004414 DNA Proteins 0.000 description 9
• 101150082854 Mertk gene Proteins 0.000 description 9
• 239000003242 anti bacterial agent Substances 0.000 description 9
• 229910002091 carbon monoxide Inorganic materials 0.000 description 9
• 230000010261 cell growth Effects 0.000 description 9
• 230000003833 cell viability Effects 0.000 description 9
• 230000001413 cellular effect Effects 0.000 description 9
• 238000003501 co-culture Methods 0.000 description 9
• 238000012258 culturing Methods 0.000 description 9
• 229940079593 drug Drugs 0.000 description 9
• 229940088597 hormone Drugs 0.000 description 9
• 239000005556 hormone Substances 0.000 description 9
• 238000011534 incubation Methods 0.000 description 9
• 210000004940 nucleus Anatomy 0.000 description 9
• 238000011160 research Methods 0.000 description 9
• 238000002054 transplantation Methods 0.000 description 9
• QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 8
• 108090000663 Annexin A1 Proteins 0.000 description 8
• CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 8
• 101800004490 Endothelin-1 Proteins 0.000 description 8
• 102400000686 Endothelin-1 Human genes 0.000 description 8
• 108010010803 Gelatin Proteins 0.000 description 8
• 208000017442 Retinal disease Diseases 0.000 description 8
• 206010038923 Retinopathy Diseases 0.000 description 8
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
• 102000002938 Thrombospondin Human genes 0.000 description 8
• 108060008245 Thrombospondin Proteins 0.000 description 8
• 101800002279 Transforming growth factor beta-1 Proteins 0.000 description 8
• APKFDSVGJQXUKY-INPOYWNPSA-N amphotericin B Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-INPOYWNPSA-N 0.000 description 8
• 238000004458 analytical method Methods 0.000 description 8
• 230000001640 apoptogenic effect Effects 0.000 description 8
• 230000003115 biocidal effect Effects 0.000 description 8
• 230000030833 cell death Effects 0.000 description 8
• 238000005119 centrifugation Methods 0.000 description 8
• 239000002299 complementary DNA Substances 0.000 description 8
• 239000013078 crystal Substances 0.000 description 8
• 239000012634 fragment Substances 0.000 description 8
• 229920000159 gelatin Polymers 0.000 description 8
• 235000019322 gelatine Nutrition 0.000 description 8
• 235000011852 gelatine desserts Nutrition 0.000 description 8
• 239000007924 injection Substances 0.000 description 8
• 238000002347 injection Methods 0.000 description 8
• 230000008774 maternal effect Effects 0.000 description 8
• 108020004999 messenger RNA Proteins 0.000 description 8
• 210000005036 nerve Anatomy 0.000 description 8
• 239000008188 pellet Substances 0.000 description 8
• 239000013641 positive control Substances 0.000 description 8
• 210000001525 retina Anatomy 0.000 description 8
• MDKGKXOCJGEUJW-UHFFFAOYSA-N suprofen Chemical compound C1=CC(C(C(O)=O)C)=CC=C1C(=O)C1=CC=CS1 MDKGKXOCJGEUJW-UHFFFAOYSA-N 0.000 description 8
• 230000004083 survival effect Effects 0.000 description 8
• 238000012546 transfer Methods 0.000 description 8
• DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 8
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 7
• NCYCYZXNIZJOKI-IOUUIBBYSA-N 11-cis-retinal Chemical compound O=C/C=C(\C)/C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-IOUUIBBYSA-N 0.000 description 7
• 239000012109 Alexa Fluor 568 Substances 0.000 description 7
• APKFDSVGJQXUKY-KKGHZKTASA-N Amphotericin-B Natural products O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1C=CC=CC=CC=CC=CC=CC=C[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-KKGHZKTASA-N 0.000 description 7
• 102100030802 Beta-2-glycoprotein 1 Human genes 0.000 description 7
• 201000004569 Blindness Diseases 0.000 description 7
• 102000003951 Erythropoietin Human genes 0.000 description 7
• 108090000394 Erythropoietin Proteins 0.000 description 7
• 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 7
• 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 7
• 102100037362 Fibronectin Human genes 0.000 description 7
• 101000924488 Homo sapiens Atrial natriuretic peptide receptor 3 Proteins 0.000 description 7
• 101001034314 Homo sapiens Lactadherin Proteins 0.000 description 7
• 102100040756 Rhodopsin Human genes 0.000 description 7
• 108090000820 Rhodopsin Proteins 0.000 description 7
• 230000000735 allogeneic effect Effects 0.000 description 7
• 229960003942 amphotericin b Drugs 0.000 description 7
• 108010023562 beta 2-Glycoprotein I Proteins 0.000 description 7
• 230000027455 binding Effects 0.000 description 7
• 239000003153 chemical reaction reagent Substances 0.000 description 7
• 239000012228 culture supernatant Substances 0.000 description 7
• 230000002950 deficient Effects 0.000 description 7
• 229940105423 erythropoietin Drugs 0.000 description 7
• 239000008273 gelatin Substances 0.000 description 7
• 102000051386 human MFGE8 Human genes 0.000 description 7
• 239000003112 inhibitor Substances 0.000 description 7
• 239000007788 liquid Substances 0.000 description 7
• 239000011159 matrix material Substances 0.000 description 7
• 230000035772 mutation Effects 0.000 description 7
• OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 7
• 230000001105 regulatory effect Effects 0.000 description 7
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
• KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 6
• 108010045374 CD36 Antigens Proteins 0.000 description 6
• 102000053028 CD36 Antigens Human genes 0.000 description 6
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
• 101000739876 Homo sapiens Brain-derived neurotrophic factor Proteins 0.000 description 6
• KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 6
• XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 6
• PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 6
• 239000004677 Nylon Substances 0.000 description 6
• 208000007014 Retinitis pigmentosa Diseases 0.000 description 6
• 102000011117 Transforming Growth Factor beta2 Human genes 0.000 description 6
• 101800000304 Transforming growth factor beta-2 Proteins 0.000 description 6
• 229960004308 acetylcysteine Drugs 0.000 description 6
• 230000004913 activation Effects 0.000 description 6
• 230000001154 acute effect Effects 0.000 description 6
• 239000003429 antifungal agent Substances 0.000 description 6
• 239000003599 detergent Substances 0.000 description 6
• 230000029087 digestion Effects 0.000 description 6
• 230000002255 enzymatic effect Effects 0.000 description 6
• 238000000684 flow cytometry Methods 0.000 description 6
• 230000006870 function Effects 0.000 description 6
• 239000000499 gel Substances 0.000 description 6
• 239000006481 glucose medium Substances 0.000 description 6
• 102000051542 human BDNF Human genes 0.000 description 6
• 229940077456 human brain-derived neurotrophic factor Drugs 0.000 description 6
• 229920002674 hyaluronan Polymers 0.000 description 6
• 229960003160 hyaluronic acid Drugs 0.000 description 6
• 210000004379 membrane Anatomy 0.000 description 6
• 239000012528 membrane Substances 0.000 description 6
• 210000002569 neuron Anatomy 0.000 description 6
• 229920001778 nylon Polymers 0.000 description 6
• 239000002245 particle Substances 0.000 description 6
• 210000001778 pluripotent stem cell Anatomy 0.000 description 6
• 238000011069 regeneration method Methods 0.000 description 6
• 238000012552 review Methods 0.000 description 6
• 239000012679 serum free medium Substances 0.000 description 6
• 230000001225 therapeutic effect Effects 0.000 description 6
• VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 6
• 230000035899 viability Effects 0.000 description 6
• ZUBDGKVDJUIMQQ-VVTNISDDSA-N CC[C@H](C)[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@@H]1CSSC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H]2CSSC[C@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC2=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](Cc2ccc(O)cc2)C(=O)N[C@H](Cc2ccccc2)C(=O)N1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(O)=O Chemical compound CC[C@H](C)[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@@H]1CSSC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H]2CSSC[C@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC2=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](Cc2ccc(O)cc2)C(=O)N[C@H](Cc2ccccc2)C(=O)N1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(O)=O ZUBDGKVDJUIMQQ-VVTNISDDSA-N 0.000 description 5
• 101800003838 Epidermal growth factor Proteins 0.000 description 5
• 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 5
• 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 5
• 108010067306 Fibronectins Proteins 0.000 description 5
• 108010024636 Glutathione Proteins 0.000 description 5
• 239000012981 Hank's balanced salt solution Substances 0.000 description 5
• 101000925493 Homo sapiens Endothelin-1 Proteins 0.000 description 5
• 101500025614 Homo sapiens Transforming growth factor beta-1 Proteins 0.000 description 5
• VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 5
• 231100000002 MTT assay Toxicity 0.000 description 5
• 238000000134 MTT assay Methods 0.000 description 5
• 102000035092 Neutral proteases Human genes 0.000 description 5
• 108091005507 Neutral proteases Proteins 0.000 description 5
• 102100025386 Oxidized low-density lipoprotein receptor 1 Human genes 0.000 description 5
• 102100033237 Pro-epidermal growth factor Human genes 0.000 description 5
• 102100021923 Prolow-density lipoprotein receptor-related protein 1 Human genes 0.000 description 5
• 101150098329 Tyro3 gene Proteins 0.000 description 5
• 102100027548 WW domain-containing transcription regulator protein 1 Human genes 0.000 description 5
• 101710088302 WW domain-containing transcription regulator protein 1 Proteins 0.000 description 5
• 239000013543 active substance Substances 0.000 description 5
• 230000002424 anti-apoptotic effect Effects 0.000 description 5
• 229940088710 antibiotic agent Drugs 0.000 description 5
• 229940121375 antifungal agent Drugs 0.000 description 5
• 230000009286 beneficial effect Effects 0.000 description 5
• 239000000872 buffer Substances 0.000 description 5
• 210000000170 cell membrane Anatomy 0.000 description 5
• 239000006285 cell suspension Substances 0.000 description 5
• 239000011248 coating agent Substances 0.000 description 5
• 238000000576 coating method Methods 0.000 description 5
• 238000011161 development Methods 0.000 description 5
• 229940116977 epidermal growth factor Drugs 0.000 description 5
• 210000000981 epithelium Anatomy 0.000 description 5
• 238000004108 freeze drying Methods 0.000 description 5
• 239000012737 fresh medium Substances 0.000 description 5
• 239000011521 glass Substances 0.000 description 5
• 229960003180 glutathione Drugs 0.000 description 5
• 238000010166 immunofluorescence Methods 0.000 description 5
• 239000007943 implant Substances 0.000 description 5
• 210000003205 muscle Anatomy 0.000 description 5
• 235000016709 nutrition Nutrition 0.000 description 5
• 230000037361 pathway Effects 0.000 description 5
• 210000001539 phagocyte Anatomy 0.000 description 5
• 238000011533 pre-incubation Methods 0.000 description 5
• 230000000750 progressive effect Effects 0.000 description 5
• 230000008929 regeneration Effects 0.000 description 5
• 230000008439 repair process Effects 0.000 description 5
• 238000009256 replacement therapy Methods 0.000 description 5
• 150000003839 salts Chemical class 0.000 description 5
• 238000012163 sequencing technique Methods 0.000 description 5
• 239000011550 stock solution Substances 0.000 description 5
• 239000000758 substrate Substances 0.000 description 5
• 238000002560 therapeutic procedure Methods 0.000 description 5
• 238000001890 transfection Methods 0.000 description 5
• AZKSAVLVSZKNRD-UHFFFAOYSA-M 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Chemical compound [Br-].S1C(C)=C(C)N=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 AZKSAVLVSZKNRD-UHFFFAOYSA-M 0.000 description 4
• 102000007469 Actins Human genes 0.000 description 4
• 108010085238 Actins Proteins 0.000 description 4
• 102000004145 Annexin A1 Human genes 0.000 description 4
• 102100040006 Annexin A1 Human genes 0.000 description 4
• 102100034605 Atrial natriuretic peptide receptor 3 Human genes 0.000 description 4
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
• 108010014258 Elastin Proteins 0.000 description 4
• 241000196324 Embryophyta Species 0.000 description 4
• 101000599951 Homo sapiens Insulin-like growth factor I Proteins 0.000 description 4
• 108010003272 Hyaluronate lyase Proteins 0.000 description 4
• 102000001974 Hyaluronidases Human genes 0.000 description 4
• 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 4
• 102100037852 Insulin-like growth factor I Human genes 0.000 description 4
• 102000007547 Laminin Human genes 0.000 description 4
• 108010085895 Laminin Proteins 0.000 description 4
• 102000005741 Metalloproteases Human genes 0.000 description 4
• 108010006035 Metalloproteases Proteins 0.000 description 4
• 102000002452 NPR3 Human genes 0.000 description 4
• 108010025020 Nerve Growth Factor Proteins 0.000 description 4
• 102000007072 Nerve Growth Factors Human genes 0.000 description 4
• 229920000954 Polyglycolide Polymers 0.000 description 4
• 108010007127 Pulmonary Surfactant-Associated Protein D Proteins 0.000 description 4
• 102100027845 Pulmonary surfactant-associated protein D Human genes 0.000 description 4
• 108090000783 Renin Proteins 0.000 description 4
• 102100028255 Renin Human genes 0.000 description 4
• 102000013275 Somatomedins Human genes 0.000 description 4
• 102000007000 Tenascin Human genes 0.000 description 4
• 108010008125 Tenascin Proteins 0.000 description 4
• 108091009550 Vesicle-associated membrane protein 5 Proteins 0.000 description 4
• 102100035071 Vimentin Human genes 0.000 description 4
• 108010065472 Vimentin Proteins 0.000 description 4
• 238000002835 absorbance Methods 0.000 description 4
• 230000032683 aging Effects 0.000 description 4
• 239000002260 anti-inflammatory agent Substances 0.000 description 4
• 229940121363 anti-inflammatory agent Drugs 0.000 description 4
• 230000001857 anti-mycotic effect Effects 0.000 description 4
• 239000002543 antimycotic Substances 0.000 description 4
• QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
• 230000015572 biosynthetic process Effects 0.000 description 4
• 230000001684 chronic effect Effects 0.000 description 4
• 210000004087 cornea Anatomy 0.000 description 4
• 229960002433 cysteine Drugs 0.000 description 4
• 230000007850 degeneration Effects 0.000 description 4
• 230000018109 developmental process Effects 0.000 description 4
• 239000000975 dye Substances 0.000 description 4
• 239000012530 fluid Substances 0.000 description 4
• MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 4
• 229960002773 hyaluronidase Drugs 0.000 description 4
• 230000028993 immune response Effects 0.000 description 4
• 230000001506 immunosuppresive effect Effects 0.000 description 4
• 238000002513 implantation Methods 0.000 description 4
• 238000011065 in-situ storage Methods 0.000 description 4
• 208000014674 injury Diseases 0.000 description 4
• 238000002372 labelling Methods 0.000 description 4
• 230000003902 lesion Effects 0.000 description 4
• 210000001161 mammalian embryo Anatomy 0.000 description 4
• 239000003550 marker Substances 0.000 description 4
• 239000003900 neurotrophic factor Substances 0.000 description 4
• 229960000988 nystatin Drugs 0.000 description 4
• VQOXZBDYSJBXMA-NQTDYLQESA-N nystatin A1 Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/CC/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 VQOXZBDYSJBXMA-NQTDYLQESA-N 0.000 description 4
• 210000004248 oligodendroglia Anatomy 0.000 description 4
• 210000000056 organ Anatomy 0.000 description 4
• 229910052760 oxygen Inorganic materials 0.000 description 4
• 239000001301 oxygen Substances 0.000 description 4
• 238000011002 quantification Methods 0.000 description 4
• 230000001172 regenerating effect Effects 0.000 description 4
• 230000003248 secreting effect Effects 0.000 description 4
• 230000009758 senescence Effects 0.000 description 4
• 210000001626 skin fibroblast Anatomy 0.000 description 4
• 239000011780 sodium chloride Substances 0.000 description 4
• GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 4
• 238000010561 standard procedure Methods 0.000 description 4
• 210000004500 stellate cell Anatomy 0.000 description 4
• 230000004936 stimulating effect Effects 0.000 description 4
• BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 4
• 230000008685 targeting Effects 0.000 description 4
• 230000017423 tissue regeneration Effects 0.000 description 4
• 210000005048 vimentin Anatomy 0.000 description 4
• 230000000007 visual effect Effects 0.000 description 4
• 230000004393 visual impairment Effects 0.000 description 4
• 238000005406 washing Methods 0.000 description 4
• FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 3
• 108010087905 Adenovirus E1B Proteins Proteins 0.000 description 3
• 108090000145 Bacillolysin Proteins 0.000 description 3
• 102100023698 C-C motif chemokine 17 Human genes 0.000 description 3
• 108010008951 Chemokine CXCL12 Proteins 0.000 description 3
• 102100032765 Chordin-like protein 1 Human genes 0.000 description 3
• 101710173231 Chordin-like protein 1 Proteins 0.000 description 3
• 102000016911 Deoxyribonucleases Human genes 0.000 description 3
• 108010053770 Deoxyribonucleases Proteins 0.000 description 3
• 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 3
• 238000012286 ELISA Assay Methods 0.000 description 3
• 102000016942 Elastin Human genes 0.000 description 3
• WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
• 101000978362 Homo sapiens C-C motif chemokine 17 Proteins 0.000 description 3
• 101001043564 Homo sapiens Prolow-density lipoprotein receptor-related protein 1 Proteins 0.000 description 3
• ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 3
• 108010052014 Liberase Proteins 0.000 description 3
• 241001465754 Metazoa Species 0.000 description 3
• DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
• 239000004365 Protease Substances 0.000 description 3
• 229940096437 Protein S Drugs 0.000 description 3
• 108010066124 Protein S Proteins 0.000 description 3
• RWRDLPDLKQPQOW-UHFFFAOYSA-N Pyrrolidine Chemical compound C1CCNC1 RWRDLPDLKQPQOW-UHFFFAOYSA-N 0.000 description 3
• 241000220317 Rosa Species 0.000 description 3
• 102000004142 Trypsin Human genes 0.000 description 3
• 102000004243 Tubulin Human genes 0.000 description 3
• 108090000704 Tubulin Proteins 0.000 description 3
• 102100028885 Vitamin K-dependent protein S Human genes 0.000 description 3
• 208000027418 Wounds and injury Diseases 0.000 description 3
• 229960000583 acetic acid Drugs 0.000 description 3
• 239000002253 acid Substances 0.000 description 3
• 210000004504 adult stem cell Anatomy 0.000 description 3
• 230000003321 amplification Effects 0.000 description 3
• 230000003078 antioxidant effect Effects 0.000 description 3
• 235000010323 ascorbic acid Nutrition 0.000 description 3
• 229960005070 ascorbic acid Drugs 0.000 description 3
• 239000011668 ascorbic acid Substances 0.000 description 3
• 239000007640 basal medium Substances 0.000 description 3
• 230000008901 benefit Effects 0.000 description 3
• 230000024245 cell differentiation Effects 0.000 description 3
• 230000032823 cell division Effects 0.000 description 3
• 238000002659 cell therapy Methods 0.000 description 3
• 210000003169 central nervous system Anatomy 0.000 description 3
• 239000006184 cosolvent Substances 0.000 description 3
• 230000007812 deficiency Effects 0.000 description 3
• 102000038379 digestive enzymes Human genes 0.000 description 3
• 108091007734 digestive enzymes Proteins 0.000 description 3
• 208000035475 disorder Diseases 0.000 description 3
• 238000010494 dissociation reaction Methods 0.000 description 3
• 230000005593 dissociations Effects 0.000 description 3
• 238000012137 double-staining Methods 0.000 description 3
• 230000003828 downregulation Effects 0.000 description 3
• 229920002549 elastin Polymers 0.000 description 3
• 230000008030 elimination Effects 0.000 description 3
• 238000003379 elimination reaction Methods 0.000 description 3
• 210000002889 endothelial cell Anatomy 0.000 description 3
• 239000003623 enhancer Substances 0.000 description 3
• 210000002919 epithelial cell Anatomy 0.000 description 3
• 238000010195 expression analysis Methods 0.000 description 3
• 238000000605 extraction Methods 0.000 description 3
• 238000001943 fluorescence-activated cell sorting Methods 0.000 description 3
• 239000006260 foam Substances 0.000 description 3
• 238000003197 gene knockdown Methods 0.000 description 3
• 238000012239 gene modification Methods 0.000 description 3
• 230000002068 genetic effect Effects 0.000 description 3
• 102000057308 human HGF Human genes 0.000 description 3
• 230000001771 impaired effect Effects 0.000 description 3
• 238000011081 inoculation Methods 0.000 description 3
• 230000003993 interaction Effects 0.000 description 3
• 239000010410 layer Substances 0.000 description 3
• 108010046018 leukocyte inhibitory factor Proteins 0.000 description 3
• 150000002632 lipids Chemical class 0.000 description 3
• 230000000510 mucolytic effect Effects 0.000 description 3
• 210000001178 neural stem cell Anatomy 0.000 description 3
• 230000000324 neuroprotective effect Effects 0.000 description 3
• 229940021182 non-steroidal anti-inflammatory drug Drugs 0.000 description 3
• 238000003199 nucleic acid amplification method Methods 0.000 description 3
• 235000003170 nutritional factors Nutrition 0.000 description 3
• 210000001328 optic nerve Anatomy 0.000 description 3
• 230000003204 osmotic effect Effects 0.000 description 3
• 229920001610 polycaprolactone Polymers 0.000 description 3
• 239000003755 preservative agent Substances 0.000 description 3
• 230000035755 proliferation Effects 0.000 description 3
• 150000003254 radicals Chemical class 0.000 description 3
• 230000010076 replication Effects 0.000 description 3
• 108091005418 scavenger receptor class E Proteins 0.000 description 3
• 230000019491 signal transduction Effects 0.000 description 3
• 230000011664 signaling Effects 0.000 description 3
• 239000013589 supplement Substances 0.000 description 3
• 230000000699 topical effect Effects 0.000 description 3
• 108091006108 transcriptional coactivators Proteins 0.000 description 3
• 238000012795 verification Methods 0.000 description 3
• 239000013603 viral vector Substances 0.000 description 3
• 230000004382 visual function Effects 0.000 description 3
• 235000019154 vitamin C Nutrition 0.000 description 3
• 239000011718 vitamin C Substances 0.000 description 3
• 235000019165 vitamin E Nutrition 0.000 description 3
• 239000011709 vitamin E Substances 0.000 description 3
• GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
• IICCLYANAQEHCI-UHFFFAOYSA-N 4,5,6,7-tetrachloro-3',6'-dihydroxy-2',4',5',7'-tetraiodospiro[2-benzofuran-3,9'-xanthene]-1-one Chemical compound O1C(=O)C(C(=C(Cl)C(Cl)=C2Cl)Cl)=C2C21C1=CC(I)=C(O)C(I)=C1OC1=C(I)C(O)=C(I)C=C21 IICCLYANAQEHCI-UHFFFAOYSA-N 0.000 description 2
• 102100024394 Adipocyte enhancer-binding protein 1 Human genes 0.000 description 2
• 101710105604 Adipocyte enhancer-binding protein 1 Proteins 0.000 description 2
• 102000000412 Annexin Human genes 0.000 description 2
• 108050008874 Annexin Proteins 0.000 description 2
• IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
• 102000004954 Biglycan Human genes 0.000 description 2
• 108090001138 Biglycan Proteins 0.000 description 2
• 102100021943 C-C motif chemokine 2 Human genes 0.000 description 2
• 102100026194 C-type lectin domain family 2 member B Human genes 0.000 description 2
• OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
• 102100029968 Calreticulin Human genes 0.000 description 2
• 108090000549 Calreticulin Proteins 0.000 description 2
• CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
• 108091006146 Channels Proteins 0.000 description 2
• 102000000013 Chemokine CCL3 Human genes 0.000 description 2
• 108050000299 Chemokine receptor Proteins 0.000 description 2
• 102000019034 Chemokines Human genes 0.000 description 2
• 102000001187 Collagen Type III Human genes 0.000 description 2
• 108010069502 Collagen Type III Proteins 0.000 description 2
• 108090000909 Collectins Proteins 0.000 description 2
• 102000004405 Collectins Human genes 0.000 description 2
• 102000010970 Connexin Human genes 0.000 description 2
• 108050001175 Connexin Proteins 0.000 description 2
• 108010079362 Core Binding Factor Alpha 3 Subunit Proteins 0.000 description 2
• 101710088434 Cytochrome c oxidase subunit 7A1, mitochondrial Proteins 0.000 description 2
• 102100025629 Cytochrome c oxidase subunit 7A1, mitochondrial Human genes 0.000 description 2
• 102000004127 Cytokines Human genes 0.000 description 2
• 108090000695 Cytokines Proteins 0.000 description 2
• 238000000116 DAPI staining Methods 0.000 description 2
• 102100037840 Dehydrogenase/reductase SDR family member 2, mitochondrial Human genes 0.000 description 2
• 206010012689 Diabetic retinopathy Diseases 0.000 description 2
• 102000001301 EGF receptor Human genes 0.000 description 2
• 108060006698 EGF receptor Proteins 0.000 description 2
• 102100021717 Early growth response protein 3 Human genes 0.000 description 2
• 108010055191 EphA3 Receptor Proteins 0.000 description 2
• 102100030324 Ephrin type-A receptor 3 Human genes 0.000 description 2
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
• AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 description 2
• 102000003886 Glycoproteins Human genes 0.000 description 2
• 108090000288 Glycoproteins Proteins 0.000 description 2
• 102100028967 HLA class I histocompatibility antigen, alpha chain G Human genes 0.000 description 2
• 108010024164 HLA-G Antigens Proteins 0.000 description 2
• 229940121710 HMGCoA reductase inhibitor Drugs 0.000 description 2
• 102100039165 Heat shock protein beta-1 Human genes 0.000 description 2
• 101710100504 Heat shock protein beta-1 Proteins 0.000 description 2
• HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
• 101800001649 Heparin-binding EGF-like growth factor Proteins 0.000 description 2
• 102000052396 Hephaestin Human genes 0.000 description 2
• 108700038053 Hephaestin Proteins 0.000 description 2
• 101100118545 Holotrichia diomphalia EGF-like gene Proteins 0.000 description 2
• 108700005087 Homeobox Genes Proteins 0.000 description 2
• 102100037102 Homeobox protein MOX-2 Human genes 0.000 description 2
• 101710142888 Homeobox protein MOX-2 Proteins 0.000 description 2
• 102100029279 Homeobox protein SIX1 Human genes 0.000 description 2
• 241000282412 Homo Species 0.000 description 2
• 101000912618 Homo sapiens C-type lectin domain family 2 member B Proteins 0.000 description 2
• 101001027128 Homo sapiens Fibronectin Proteins 0.000 description 2
• 101000997829 Homo sapiens Glial cell line-derived neurotrophic factor Proteins 0.000 description 2
• 101001044940 Homo sapiens Insulin-like growth factor-binding protein 2 Proteins 0.000 description 2
• 101001003147 Homo sapiens Interleukin-11 receptor subunit alpha Proteins 0.000 description 2
• 101000934372 Homo sapiens Macrosialin Proteins 0.000 description 2
• 101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 description 2
• 101000904196 Homo sapiens Pancreatic secretory granule membrane major glycoprotein GP2 Proteins 0.000 description 2
• 101000612134 Homo sapiens Procollagen C-endopeptidase enhancer 1 Proteins 0.000 description 2
• 101000736906 Homo sapiens Protein prune homolog 2 Proteins 0.000 description 2
• 101000632467 Homo sapiens Pulmonary surfactant-associated protein D Proteins 0.000 description 2
• 101000890554 Homo sapiens Retinal dehydrogenase 2 Proteins 0.000 description 2
• 101000914484 Homo sapiens T-lymphocyte activation antigen CD80 Proteins 0.000 description 2
• 101000830570 Homo sapiens Tumor necrosis factor alpha-induced protein 3 Proteins 0.000 description 2
• 101000803709 Homo sapiens Vitronectin Proteins 0.000 description 2
• 101000964749 Homo sapiens Zinc finger protein 710 Proteins 0.000 description 2
• 102100034980 ICOS ligand Human genes 0.000 description 2
• 206010062016 Immunosuppression Diseases 0.000 description 2
• 102000048143 Insulin-Like Growth Factor II Human genes 0.000 description 2
• 108090001117 Insulin-Like Growth Factor II Proteins 0.000 description 2
• 102100022710 Insulin-like growth factor-binding protein 2 Human genes 0.000 description 2
• 102100033016 Integrin beta-7 Human genes 0.000 description 2
• 102100033336 Integrin beta-8 Human genes 0.000 description 2
• 102100020787 Interleukin-11 receptor subunit alpha Human genes 0.000 description 2
• XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
• 102100023529 Iroquois-class homeodomain protein IRX-5 Human genes 0.000 description 2
• 101710136127 Iroquois-class homeodomain protein IRX-5 Proteins 0.000 description 2
• 102100033420 Keratin, type I cytoskeletal 19 Human genes 0.000 description 2
• 239000004201 L-cysteine Substances 0.000 description 2
• 235000013878 L-cysteine Nutrition 0.000 description 2
• 229930182821 L-proline Natural products 0.000 description 2
• 108010007622 LDL Lipoproteins Proteins 0.000 description 2
• 102000007330 LDL Lipoproteins Human genes 0.000 description 2
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
• 239000004472 Lysine Substances 0.000 description 2
• 241000829100 Macaca mulatta polyomavirus 1 Species 0.000 description 2
• 102100034184 Macrophage scavenger receptor types I and II Human genes 0.000 description 2
• 101710134306 Macrophage scavenger receptor types I and II Proteins 0.000 description 2
• 102100025136 Macrosialin Human genes 0.000 description 2
• 241000124008 Mammalia Species 0.000 description 2
• 108010031099 Mannose Receptor Proteins 0.000 description 2
• 102000018697 Membrane Proteins Human genes 0.000 description 2
• 108010052285 Membrane Proteins Proteins 0.000 description 2
• 102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 description 2
• 101100262697 Mus musculus Axl gene Proteins 0.000 description 2
• 101100407308 Mus musculus Pdcd1lg2 gene Proteins 0.000 description 2
• 102000008730 Nestin Human genes 0.000 description 2
• 108010088225 Nestin Proteins 0.000 description 2
• 206010029260 Neuroblastoma Diseases 0.000 description 2
• 101710163270 Nuclease Proteins 0.000 description 2
• 206010033128 Ovarian cancer Diseases 0.000 description 2
• 206010061535 Ovarian neoplasm Diseases 0.000 description 2
• 101710199789 Oxidized low-density lipoprotein receptor 1 Proteins 0.000 description 2
• 102000004316 Oxidoreductases Human genes 0.000 description 2
• 108090000854 Oxidoreductases Proteins 0.000 description 2
• 102100024019 Pancreatic secretory granule membrane major glycoprotein GP2 Human genes 0.000 description 2
• 241001631646 Papillomaviridae Species 0.000 description 2
• 229930040373 Paraformaldehyde Natural products 0.000 description 2
• 102000035195 Peptidases Human genes 0.000 description 2
• 108091005804 Peptidases Proteins 0.000 description 2
• NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
• 108091000080 Phosphotransferase Proteins 0.000 description 2
• 102100040681 Platelet-derived growth factor C Human genes 0.000 description 2
• 239000002202 Polyethylene glycol Substances 0.000 description 2
• 229920001213 Polysorbate 20 Polymers 0.000 description 2
• 239000004793 Polystyrene Substances 0.000 description 2
• ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
• 102100041026 Procollagen C-endopeptidase enhancer 1 Human genes 0.000 description 2
• 102100038931 Proenkephalin-A Human genes 0.000 description 2
• 108700030875 Programmed Cell Death 1 Ligand 2 Proteins 0.000 description 2
• 102100024213 Programmed cell death 1 ligand 2 Human genes 0.000 description 2
• 101710202113 Prolow-density lipoprotein receptor-related protein 1 Proteins 0.000 description 2
• 101710188053 Protein D Proteins 0.000 description 2
• 102100021538 Protein kinase C zeta type Human genes 0.000 description 2
• 102100036040 Protein prune homolog 2 Human genes 0.000 description 2
• KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 description 2
• 238000002123 RNA extraction Methods 0.000 description 2
• 239000013614 RNA sample Substances 0.000 description 2
• 101710132893 Resolvase Proteins 0.000 description 2
• 102100040070 Retinal dehydrogenase 2 Human genes 0.000 description 2
• 102100025369 Runt-related transcription factor 3 Human genes 0.000 description 2
• 102100021669 Stromal cell-derived factor 1 Human genes 0.000 description 2
• 238000003639 Student–Newman–Keuls (SNK) method Methods 0.000 description 2
• QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
• 102100027222 T-lymphocyte activation antigen CD80 Human genes 0.000 description 2
• 102000004887 Transforming Growth Factor beta Human genes 0.000 description 2
• 108090001012 Transforming Growth Factor beta Proteins 0.000 description 2
• GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 2
• 108090000631 Trypsin Proteins 0.000 description 2
• 102100024596 Tumor necrosis factor alpha-induced protein 3 Human genes 0.000 description 2
• 102100031988 Tumor necrosis factor ligand superfamily member 6 Human genes 0.000 description 2
• 102000033162 Vesicle-associated membrane protein 5 Human genes 0.000 description 2
• 102100031484 Vesicle-associated membrane protein 5 Human genes 0.000 description 2
• 102100025342 Voltage-dependent N-type calcium channel subunit alpha-1B Human genes 0.000 description 2
• 101710088658 Voltage-dependent N-type calcium channel subunit alpha-1B Proteins 0.000 description 2
• 208000008383 Wilms tumor Diseases 0.000 description 2
• 208000026448 Wilms tumor 1 Diseases 0.000 description 2
• 102100022748 Wilms tumor protein Human genes 0.000 description 2
• 101710127857 Wilms tumor protein Proteins 0.000 description 2
• 230000005856 abnormality Effects 0.000 description 2
• 239000012190 activator Substances 0.000 description 2
• 239000002671 adjuvant Substances 0.000 description 2
• 150000001298 alcohols Chemical class 0.000 description 2
• 102000013640 alpha-Crystallin B Chain Human genes 0.000 description 2
• 108010051585 alpha-Crystallin B Chain Proteins 0.000 description 2
• 230000019552 anatomical structure morphogenesis Effects 0.000 description 2
• 210000002159 anterior chamber Anatomy 0.000 description 2
• 230000003110 anti-inflammatory effect Effects 0.000 description 2
• 230000001028 anti-proliverative effect Effects 0.000 description 2
• 206010002906 aortic stenosis Diseases 0.000 description 2
• 239000003181 biological factor Substances 0.000 description 2
• 239000012620 biological material Substances 0.000 description 2
• 210000001775 bruch membrane Anatomy 0.000 description 2
• 229940046731 calcineurin inhibitors Drugs 0.000 description 2
• 239000011575 calcium Substances 0.000 description 2
• 229910052791 calcium Inorganic materials 0.000 description 2
• 210000003986 cell retinal photoreceptor Anatomy 0.000 description 2
• 238000003570 cell viability assay Methods 0.000 description 2
• 230000010094 cellular senescence Effects 0.000 description 2
• 239000003638 chemical reducing agent Substances 0.000 description 2
• OSASVXMJTNOKOY-UHFFFAOYSA-N chlorobutanol Chemical compound CC(C)(O)C(Cl)(Cl)Cl OSASVXMJTNOKOY-UHFFFAOYSA-N 0.000 description 2
• HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
• 210000001136 chorion Anatomy 0.000 description 2
• 238000004140 cleaning Methods 0.000 description 2
• ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 description 2
• 238000007796 conventional method Methods 0.000 description 2
• 229920001577 copolymer Polymers 0.000 description 2
• 239000003246 corticosteroid Substances 0.000 description 2
• 229960001334 corticosteroids Drugs 0.000 description 2
• 238000005138 cryopreservation Methods 0.000 description 2
• 210000004748 cultured cell Anatomy 0.000 description 2
• XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 2
• 235000018417 cysteine Nutrition 0.000 description 2
• 231100000135 cytotoxicity Toxicity 0.000 description 2
• 230000003013 cytotoxicity Effects 0.000 description 2
• 230000007547 defect Effects 0.000 description 2
• 230000001419 dependent effect Effects 0.000 description 2
• 230000008021 deposition Effects 0.000 description 2
• 239000003085 diluting agent Substances 0.000 description 2
• 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 2
• 230000008034 disappearance Effects 0.000 description 2
• 239000012153 distilled water Substances 0.000 description 2
• 238000009826 distribution Methods 0.000 description 2
• 239000002552 dosage form Substances 0.000 description 2
• 238000012377 drug delivery Methods 0.000 description 2
• 238000001035 drying Methods 0.000 description 2
• 238000001962 electrophoresis Methods 0.000 description 2
• 210000002308 embryonic cell Anatomy 0.000 description 2
• 210000001671 embryonic stem cell Anatomy 0.000 description 2
• 239000003889 eye drop Substances 0.000 description 2
• 239000000835 fiber Substances 0.000 description 2
• 239000000706 filtrate Substances 0.000 description 2
• 238000001914 filtration Methods 0.000 description 2
• 239000012520 frozen sample Substances 0.000 description 2
• 238000003209 gene knockout Methods 0.000 description 2
• 230000005017 genetic modification Effects 0.000 description 2
• 235000013617 genetically modified food Nutrition 0.000 description 2
• 210000001654 germ layer Anatomy 0.000 description 2
• 210000003714 granulocyte Anatomy 0.000 description 2
• 230000035876 healing Effects 0.000 description 2
• 230000036541 health Effects 0.000 description 2
• 210000003958 hematopoietic stem cell Anatomy 0.000 description 2
• 102000052654 human GDNF Human genes 0.000 description 2
• 239000000017 hydrogel Substances 0.000 description 2
• 239000002471 hydroxymethylglutaryl coenzyme A reductase inhibitor Substances 0.000 description 2
• 230000000222 hyperoxic effect Effects 0.000 description 2
• 238000005286 illumination Methods 0.000 description 2
• 238000003384 imaging method Methods 0.000 description 2
• 230000002519 immonomodulatory effect Effects 0.000 description 2
• 238000003125 immunofluorescent labeling Methods 0.000 description 2
• 239000003018 immunosuppressive agent Substances 0.000 description 2
• 230000001939 inductive effect Effects 0.000 description 2
• 230000002757 inflammatory effect Effects 0.000 description 2
• 238000001802 infusion Methods 0.000 description 2
• 108010021315 integrin beta7 Proteins 0.000 description 2
• 108010021506 integrin beta8 Proteins 0.000 description 2
• 108010093036 interleukin receptors Proteins 0.000 description 2
• 102000002467 interleukin receptors Human genes 0.000 description 2
• JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
• 238000002647 laser therapy Methods 0.000 description 2
• 239000002502 liposome Substances 0.000 description 2
• 239000003589 local anesthetic agent Substances 0.000 description 2
• 229960005015 local anesthetics Drugs 0.000 description 2
• 230000007774 longterm Effects 0.000 description 2
• 239000006166 lysate Substances 0.000 description 2
• 229940124302 mTOR inhibitor Drugs 0.000 description 2
• 239000003628 mammalian target of rapamycin inhibitor Substances 0.000 description 2
• 230000035800 maturation Effects 0.000 description 2
• 230000007246 mechanism Effects 0.000 description 2
• 238000011177 media preparation Methods 0.000 description 2
• 201000001441 melanoma Diseases 0.000 description 2
• 230000003278 mimic effect Effects 0.000 description 2
• 239000002829 mitogen activated protein kinase inhibitor Substances 0.000 description 2
• 230000009149 molecular binding Effects 0.000 description 2
• 210000001616 monocyte Anatomy 0.000 description 2
• 210000005055 nestin Anatomy 0.000 description 2
• 230000000926 neurological effect Effects 0.000 description 2
• 239000004090 neuroprotective agent Substances 0.000 description 2
• 238000001543 one-way ANOVA Methods 0.000 description 2
• 238000005457 optimization Methods 0.000 description 2
• 102000002574 p38 Mitogen-Activated Protein Kinases Human genes 0.000 description 2
• 108010068338 p38 Mitogen-Activated Protein Kinases Proteins 0.000 description 2
• 229920002866 paraformaldehyde Polymers 0.000 description 2
• 230000036961 partial effect Effects 0.000 description 2
• HIANJWSAHKJQTH-UHFFFAOYSA-N pemirolast Chemical compound CC1=CC=CN(C2=O)C1=NC=C2C=1N=NNN=1 HIANJWSAHKJQTH-UHFFFAOYSA-N 0.000 description 2
• 229960004439 pemirolast Drugs 0.000 description 2
• 239000000137 peptide hydrolase inhibitor Substances 0.000 description 2
• 210000005259 peripheral blood Anatomy 0.000 description 2
• 239000011886 peripheral blood Substances 0.000 description 2
• 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 2
• 208000025712 phagocytic cell dysfunction Diseases 0.000 description 2
• 150000003904 phospholipids Chemical class 0.000 description 2
• 102000020233 phosphotransferase Human genes 0.000 description 2
• 238000007539 photo-oxidation reaction Methods 0.000 description 2
• 230000035479 physiological effects, processes and functions Effects 0.000 description 2
• 230000003169 placental effect Effects 0.000 description 2
• 210000002381 plasma Anatomy 0.000 description 2
• 210000004623 platelet-rich plasma Anatomy 0.000 description 2
• 229920001223 polyethylene glycol Polymers 0.000 description 2
• 229920000642 polymer Polymers 0.000 description 2
• 238000003752 polymerase chain reaction Methods 0.000 description 2
• 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
• 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
• 229920002223 polystyrene Polymers 0.000 description 2
• 229910052700 potassium Inorganic materials 0.000 description 2
• 239000011591 potassium Substances 0.000 description 2
• 230000035935 pregnancy Effects 0.000 description 2
• FYPMFJGVHOHGLL-UHFFFAOYSA-N probucol Chemical compound C=1C(C(C)(C)C)=C(O)C(C(C)(C)C)=CC=1SC(C)(C)SC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 FYPMFJGVHOHGLL-UHFFFAOYSA-N 0.000 description 2
• 229960003912 probucol Drugs 0.000 description 2
• 102000004196 processed proteins & peptides Human genes 0.000 description 2
• 108090000765 processed proteins & peptides Proteins 0.000 description 2
• 238000012545 processing Methods 0.000 description 2
• 108010041071 proenkephalin Proteins 0.000 description 2
• 229960002429 proline Drugs 0.000 description 2
• 235000019419 proteases Nutrition 0.000 description 2
• 108010050991 protein kinase C zeta Proteins 0.000 description 2
• 238000000746 purification Methods 0.000 description 2
• ZAHRKKWIAAJSAO-UHFFFAOYSA-N rapamycin Natural products COCC(O)C(=C/C(C)C(=O)CC(OC(=O)C1CCCCN1C(=O)C(=O)C2(O)OC(CC(OC)C(=CC=CC=CC(C)CC(C)C(=O)C)C)CCC2C)C(C)CC3CCC(O)C(C3)OC)C ZAHRKKWIAAJSAO-UHFFFAOYSA-N 0.000 description 2
• 238000011084 recovery Methods 0.000 description 2
• 229940116176 remicade Drugs 0.000 description 2
• BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 2
• 229930187593 rose bengal Natural products 0.000 description 2
• 229940081623 rose bengal Drugs 0.000 description 2
• STRXNPAVPKGJQR-UHFFFAOYSA-N rose bengal A Natural products O1C(=O)C(C(=CC=C2Cl)Cl)=C2C21C1=CC(I)=C(O)C(I)=C1OC1=C(I)C(O)=C(I)C=C21 STRXNPAVPKGJQR-UHFFFAOYSA-N 0.000 description 2
• 102000014452 scavenger receptors Human genes 0.000 description 2
• 108010078070 scavenger receptors Proteins 0.000 description 2
• 238000007790 scraping Methods 0.000 description 2
• 238000012216 screening Methods 0.000 description 2
• 230000001568 sexual effect Effects 0.000 description 2
• 229960002930 sirolimus Drugs 0.000 description 2
• QFJCIRLUMZQUOT-HPLJOQBZSA-N sirolimus Chemical compound C1C[C@@H](O)[C@H](OC)C[C@@H]1C[C@@H](C)[C@H]1OC(=O)[C@@H]2CCCCN2C(=O)C(=O)[C@](O)(O2)[C@H](C)CC[C@H]2C[C@H](OC)/C(C)=C/C=C/C=C/[C@@H](C)C[C@@H](C)C(=O)[C@H](OC)[C@H](O)/C(C)=C/[C@@H](C)C(=O)C1 QFJCIRLUMZQUOT-HPLJOQBZSA-N 0.000 description 2
• CSMWJXBSXGUPGY-UHFFFAOYSA-L sodium dithionate Chemical compound [Na+].[Na+].[O-]S(=O)(=O)S([O-])(=O)=O CSMWJXBSXGUPGY-UHFFFAOYSA-L 0.000 description 2
• DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 2
• 235000010265 sodium sulphite Nutrition 0.000 description 2
• 241000894007 species Species 0.000 description 2
• 229960004492 suprofen Drugs 0.000 description 2
• 238000001356 surgical procedure Methods 0.000 description 2
• 230000009747 swallowing Effects 0.000 description 2
• 210000002504 synaptic vesicle Anatomy 0.000 description 2
• 208000011580 syndromic disease Diseases 0.000 description 2
• XYKWNRUXCOIMFZ-UHFFFAOYSA-N tepoxalin Chemical compound C1=CC(OC)=CC=C1N1C(C=2C=CC(Cl)=CC=2)=CC(CCC(=O)N(C)O)=N1 XYKWNRUXCOIMFZ-UHFFFAOYSA-N 0.000 description 2
• 229950009638 tepoxalin Drugs 0.000 description 2
• ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 description 2
• 108010060887 thrombospondin 2 Proteins 0.000 description 2
• 210000001541 thymus gland Anatomy 0.000 description 2
• 230000009772 tissue formation Effects 0.000 description 2
• UPSPUYADGBWSHF-UHFFFAOYSA-N tolmetin Chemical compound C1=CC(C)=CC=C1C(=O)C1=CC=C(CC(O)=O)N1C UPSPUYADGBWSHF-UHFFFAOYSA-N 0.000 description 2
• 229960001017 tolmetin Drugs 0.000 description 2
• 238000003325 tomography Methods 0.000 description 2
• 230000001988 toxicity Effects 0.000 description 2
• 231100000419 toxicity Toxicity 0.000 description 2
• 229960005342 tranilast Drugs 0.000 description 2
• NZHGWWWHIYHZNX-CSKARUKUSA-N tranilast Chemical compound C1=C(OC)C(OC)=CC=C1\C=C\C(=O)NC1=CC=CC=C1C(O)=O NZHGWWWHIYHZNX-CSKARUKUSA-N 0.000 description 2
• 230000032258 transport Effects 0.000 description 2
• 239000012588 trypsin Substances 0.000 description 2
• 231100000588 tumorigenic Toxicity 0.000 description 2
• 230000000381 tumorigenic effect Effects 0.000 description 2
• 238000000108 ultra-filtration Methods 0.000 description 2
• 239000012130 whole-cell lysate Substances 0.000 description 2
• 239000012224 working solution Substances 0.000 description 2
• QCHFTSOMWOSFHM-WPRPVWTQSA-N (+)-Pilocarpine Chemical compound C1OC(=O)[C@@H](CC)[C@H]1CC1=CN=CN1C QCHFTSOMWOSFHM-WPRPVWTQSA-N 0.000 description 1
• DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
• ROICYBLUWUMJFF-RDTXWAMCSA-N (6aR,9R)-N,7-dimethyl-N-propan-2-yl-6,6a,8,9-tetrahydro-4H-indolo[4,3-fg]quinoline-9-carboxamide Chemical compound CN(C(=O)[C@H]1CN(C)[C@@H]2CC3=CNC4=CC=CC(C2=C1)=C34)C(C)C ROICYBLUWUMJFF-RDTXWAMCSA-N 0.000 description 1
• JCIIKRHCWVHVFF-UHFFFAOYSA-N 1,2,4-thiadiazol-5-amine;hydrochloride Chemical compound Cl.NC1=NC=NS1 JCIIKRHCWVHVFF-UHFFFAOYSA-N 0.000 description 1
• VQJIFEJETJIBDG-UHFFFAOYSA-N 1-(1-ethoxyethoxy)dodecane Chemical compound CCCCCCCCCCCCOC(C)OCC VQJIFEJETJIBDG-UHFFFAOYSA-N 0.000 description 1
• KVUXYQHEESDGIJ-UHFFFAOYSA-N 10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthrene-3,16-diol Chemical compound C1CC2CC(O)CCC2(C)C2C1C1CC(O)CC1(C)CC2 KVUXYQHEESDGIJ-UHFFFAOYSA-N 0.000 description 1
• WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
• PWRDDPBVJCMVNM-UHFFFAOYSA-N 2-(3-sulfanylpropylamino)acetic acid Chemical compound OC(=O)CNCCCS PWRDDPBVJCMVNM-UHFFFAOYSA-N 0.000 description 1
• IEQAICDLOKRSRL-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-dodecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO IEQAICDLOKRSRL-UHFFFAOYSA-N 0.000 description 1
• HNLXNOZHXNSSPN-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCOCCOCCOCCOCCOCCOCCO)C=C1 HNLXNOZHXNSSPN-UHFFFAOYSA-N 0.000 description 1
• KISWVXRQTGLFGD-UHFFFAOYSA-N 2-[[2-[[6-amino-2-[[2-[[2-[[5-amino-2-[[2-[[1-[2-[[6-amino-2-[(2,5-diamino-5-oxopentanoyl)amino]hexanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]pyrrolidine-2-carbonyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]-5-(diaminomethylideneamino)p Chemical compound C1CCN(C(=O)C(CCCN=C(N)N)NC(=O)C(CCCCN)NC(=O)C(N)CCC(N)=O)C1C(=O)NC(CO)C(=O)NC(CCC(N)=O)C(=O)NC(CCCN=C(N)N)C(=O)NC(CO)C(=O)NC(CCCCN)C(=O)NC(C(=O)NC(CC(C)C)C(O)=O)CC1=CC=C(O)C=C1 KISWVXRQTGLFGD-UHFFFAOYSA-N 0.000 description 1
• ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 1
• LCSKNASZPVZHEG-UHFFFAOYSA-N 3,6-dimethyl-1,4-dioxane-2,5-dione;1,4-dioxane-2,5-dione Chemical compound O=C1COC(=O)CO1.CC1OC(=O)C(C)OC1=O LCSKNASZPVZHEG-UHFFFAOYSA-N 0.000 description 1
• UMCMPZBLKLEWAF-BCTGSCMUSA-N 3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 UMCMPZBLKLEWAF-BCTGSCMUSA-N 0.000 description 1
• 101710172561 3alpha-hydroxysteroid dehydrogenase Proteins 0.000 description 1
• LHYQAEFVHIZFLR-UHFFFAOYSA-L 4-(4-diazonio-3-methoxyphenyl)-2-methoxybenzenediazonium;dichloride Chemical compound [Cl-].[Cl-].C1=C([N+]#N)C(OC)=CC(C=2C=C(OC)C([N+]#N)=CC=2)=C1 LHYQAEFVHIZFLR-UHFFFAOYSA-L 0.000 description 1
• 125000003143 4-hydroxybenzyl group Chemical group [H]C([*])([H])C1=C([H])C([H])=C(O[H])C([H])=C1[H] 0.000 description 1
• XZIIFPSPUDAGJM-UHFFFAOYSA-N 6-chloro-2-n,2-n-diethylpyrimidine-2,4-diamine Polymers CCN(CC)C1=NC(N)=CC(Cl)=N1 XZIIFPSPUDAGJM-UHFFFAOYSA-N 0.000 description 1
• 229920001817 Agar Polymers 0.000 description 1
• 229920000936 Agarose Polymers 0.000 description 1
• 102100036601 Aggrecan core protein Human genes 0.000 description 1
• 108010067219 Aggrecans Proteins 0.000 description 1
• 102100024089 Aldo-keto reductase family 1 member C2 Human genes 0.000 description 1
• 102100024090 Aldo-keto reductase family 1 member C3 Human genes 0.000 description 1
• 102100038910 Alpha-enolase Human genes 0.000 description 1
• 102100023635 Alpha-fetoprotein Human genes 0.000 description 1
• 229930183010 Amphotericin Natural products 0.000 description 1
• QGGFZZLFKABGNL-UHFFFAOYSA-N Amphotericin A Natural products OC1C(N)C(O)C(C)OC1OC1C=CC=CC=CC=CCCC=CC=CC(C)C(O)C(C)C(C)OC(=O)CC(O)CC(O)CCC(O)C(O)CC(O)CC(O)(CC(O)C2C(O)=O)OC2C1 QGGFZZLFKABGNL-UHFFFAOYSA-N 0.000 description 1
• 229920000856 Amylose Polymers 0.000 description 1
• 206010051999 Anogenital dysplasia Diseases 0.000 description 1
• 108020005544 Antisense RNA Proteins 0.000 description 1
• 102100021569 Apoptosis regulator Bcl-2 Human genes 0.000 description 1
• 239000012583 B-27 Supplement Substances 0.000 description 1
• 108091012583 BCL2 Proteins 0.000 description 1
• 102100035656 BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 Human genes 0.000 description 1
• 108010081589 Becaplermin Proteins 0.000 description 1
• 210000003771 C cell Anatomy 0.000 description 1
• 101710155857 C-C motif chemokine 2 Proteins 0.000 description 1
• 102100036845 C-C motif chemokine 22 Human genes 0.000 description 1
• 101710155856 C-C motif chemokine 3 Proteins 0.000 description 1
• 102100021984 C-C motif chemokine 4-like Human genes 0.000 description 1
• 102100036153 C-X-C motif chemokine 6 Human genes 0.000 description 1
• 108700012434 CCL3 Proteins 0.000 description 1
• 108010017148 CCR8 Receptors Proteins 0.000 description 1
• 102000004426 CCR8 Receptors Human genes 0.000 description 1
• 241000283707 Capra Species 0.000 description 1
• 102100035882 Catalase Human genes 0.000 description 1
• 108010053835 Catalase Proteins 0.000 description 1
• 208000009043 Chemical Burns Diseases 0.000 description 1
• 108010083701 Chemokine CCL22 Proteins 0.000 description 1
• 108010055165 Chemokine CCL4 Proteins 0.000 description 1
• 102000001327 Chemokine CCL5 Human genes 0.000 description 1
• 102000006573 Chemokine CXCL12 Human genes 0.000 description 1
• 108010014423 Chemokine CXCL6 Proteins 0.000 description 1
• 102000009410 Chemokine receptor Human genes 0.000 description 1
• 108010012236 Chemokines Proteins 0.000 description 1
• 241000819038 Chichester Species 0.000 description 1
• 108010010786 Cholesterol 25-hydroxylase Proteins 0.000 description 1
• 229920001287 Chondroitin sulfate Polymers 0.000 description 1
• 208000017667 Chronic Disease Diseases 0.000 description 1
• 108090000317 Chymotrypsin Proteins 0.000 description 1
• 206010010356 Congenital anomaly Diseases 0.000 description 1
• 108010002947 Connectin Proteins 0.000 description 1
• 241000195493 Cryptophyta Species 0.000 description 1
• 102000002428 Cyclin C Human genes 0.000 description 1
• 108010068155 Cyclin C Proteins 0.000 description 1
• 102100026533 Cytochrome P450 1A2 Human genes 0.000 description 1
• IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
• ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
• 102100032883 DNA-binding protein SATB2 Human genes 0.000 description 1
• CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
• 208000034423 Delivery Diseases 0.000 description 1
• 229920000045 Dermatan sulfate Polymers 0.000 description 1
• 208000012184 Diffuse Brain injury Diseases 0.000 description 1
• 241001454374 Drosophila <fruit fly, subgenus> Species 0.000 description 1
• 102100031814 EGF-containing fibulin-like extracellular matrix protein 1 Human genes 0.000 description 1
• 101710176517 EGF-containing fibulin-like extracellular matrix protein 1 Proteins 0.000 description 1
• 108010051764 Early Growth Response Protein 3 Proteins 0.000 description 1
• 102000005593 Endopeptidases Human genes 0.000 description 1
• 108010059378 Endopeptidases Proteins 0.000 description 1
• 101710139422 Eotaxin Proteins 0.000 description 1
• 102100023688 Eotaxin Human genes 0.000 description 1
• 108010043938 Ephrin-A4 Proteins 0.000 description 1
• 102100033942 Ephrin-A4 Human genes 0.000 description 1
• 241000283073 Equus caballus Species 0.000 description 1
• 241000402754 Erythranthe moschata Species 0.000 description 1
• 108010054218 Factor VIII Proteins 0.000 description 1
• 102000001690 Factor VIII Human genes 0.000 description 1
• BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
• 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 1
• 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
• CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
• 229930182566 Gentamicin Natural products 0.000 description 1
• 208000010412 Glaucoma Diseases 0.000 description 1
• 102000053171 Glial Fibrillary Acidic Human genes 0.000 description 1
• 101710193519 Glial fibrillary acidic protein Proteins 0.000 description 1
• 102000053187 Glucuronidase Human genes 0.000 description 1
• 108010060309 Glucuronidase Proteins 0.000 description 1
• SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
• 229920002683 Glycosaminoglycan Polymers 0.000 description 1
• 108060003393 Granulin Proteins 0.000 description 1
• 102100031487 Growth arrest-specific protein 6 Human genes 0.000 description 1
• 241000193159 Hathewaya histolytica Species 0.000 description 1
• 102000002812 Heat-Shock Proteins Human genes 0.000 description 1
• 108010004889 Heat-Shock Proteins Proteins 0.000 description 1
• 102000003693 Hedgehog Proteins Human genes 0.000 description 1
• 108090000031 Hedgehog Proteins Proteins 0.000 description 1
• 229920002971 Heparan sulfate Polymers 0.000 description 1
• 102000009824 Hepatocyte Nuclear Factor 1-alpha Human genes 0.000 description 1
• 108010020382 Hepatocyte Nuclear Factor 1-alpha Proteins 0.000 description 1
• 208000028782 Hereditary disease Diseases 0.000 description 1
• 102100022373 Homeobox protein DLX-5 Human genes 0.000 description 1
• 101710189536 Homeobox protein SIX1 Proteins 0.000 description 1
• 102100027332 Homeobox protein SIX2 Human genes 0.000 description 1
• 101000690306 Homo sapiens Aldo-keto reductase family 1 member C3 Proteins 0.000 description 1
• 101000924533 Homo sapiens Angiopoietin-2 Proteins 0.000 description 1
• 101000959738 Homo sapiens Annexin A1 Proteins 0.000 description 1
• 101000803294 Homo sapiens BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 Proteins 0.000 description 1
• 101000655236 Homo sapiens DNA-binding protein SATB2 Proteins 0.000 description 1
• 101000896450 Homo sapiens Early growth response protein 3 Proteins 0.000 description 1
• 101000923005 Homo sapiens Growth arrest-specific protein 6 Proteins 0.000 description 1
• 101500025336 Homo sapiens Heparin-binding EGF-like growth factor Proteins 0.000 description 1
• 101000901627 Homo sapiens Homeobox protein DLX-5 Proteins 0.000 description 1
• 101000634171 Homo sapiens Homeobox protein SIX1 Proteins 0.000 description 1
• 101000651912 Homo sapiens Homeobox protein SIX2 Proteins 0.000 description 1
• 101000979760 Homo sapiens Protein NDNF Proteins 0.000 description 1
• 101000670189 Homo sapiens Ribulose-phosphate 3-epimerase Proteins 0.000 description 1
• 101000703741 Homo sapiens Short stature homeobox protein 2 Proteins 0.000 description 1
• 101000626125 Homo sapiens Tetranectin Proteins 0.000 description 1
• 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
• MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 1
• 239000004354 Hydroxyethyl cellulose Substances 0.000 description 1
• 229920000663 Hydroxyethyl cellulose Polymers 0.000 description 1
• 229920002153 Hydroxypropyl cellulose Polymers 0.000 description 1
• 108010062875 Hydroxysteroid Dehydrogenases Proteins 0.000 description 1
• 102000011145 Hydroxysteroid Dehydrogenases Human genes 0.000 description 1
• 206010020751 Hypersensitivity Diseases 0.000 description 1
• 208000026350 Inborn Genetic disease Diseases 0.000 description 1
• 206010061218 Inflammation Diseases 0.000 description 1
• 108010047852 Integrin alphaVbeta3 Proteins 0.000 description 1
• 108010002352 Interleukin-1 Proteins 0.000 description 1
• 102000015696 Interleukins Human genes 0.000 description 1
• 108010063738 Interleukins Proteins 0.000 description 1
• 239000007760 Iscove's Modified Dulbecco's Medium Substances 0.000 description 1
• 102100033421 Keratin, type I cytoskeletal 18 Human genes 0.000 description 1
• 102100023972 Keratin, type II cytoskeletal 8 Human genes 0.000 description 1
• 108010066327 Keratin-18 Proteins 0.000 description 1
• 108010066302 Keratin-19 Proteins 0.000 description 1
• 108010070511 Keratin-8 Proteins 0.000 description 1
• 102000011782 Keratins Human genes 0.000 description 1
• 108010076876 Keratins Proteins 0.000 description 1
• GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
• 102000004856 Lectins Human genes 0.000 description 1
• 108090001090 Lectins Proteins 0.000 description 1
• 102000004058 Leukemia inhibitory factor Human genes 0.000 description 1
• 108090000581 Leukemia inhibitory factor Proteins 0.000 description 1
• 208000035752 Live birth Diseases 0.000 description 1
• DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
• 206010025421 Macule Diseases 0.000 description 1
• 101710091439 Major capsid protein 1 Proteins 0.000 description 1
• 108010016113 Matrix Metalloproteinase 1 Proteins 0.000 description 1
• 102000000380 Matrix Metalloproteinase 1 Human genes 0.000 description 1
• 208000024556 Mendelian disease Diseases 0.000 description 1
• 102000003792 Metallothionein Human genes 0.000 description 1
• 108090000157 Metallothionein Proteins 0.000 description 1
• 102000047918 Myelin Basic Human genes 0.000 description 1
• 101710107068 Myelin basic protein Proteins 0.000 description 1
• 101150066297 NPR3 gene Proteins 0.000 description 1
• 229930193140 Neomycin Natural products 0.000 description 1
• 206010028980 Neoplasm Diseases 0.000 description 1
• 208000022873 Ocular disease Diseases 0.000 description 1
• 108700020796 Oncogene Proteins 0.000 description 1
• 108700022034 Opsonin Proteins Proteins 0.000 description 1
• 206010061323 Optic neuropathy Diseases 0.000 description 1
• 108010067372 Pancreatic elastase Proteins 0.000 description 1
• 102000016387 Pancreatic elastase Human genes 0.000 description 1
• 108090000526 Papain Proteins 0.000 description 1
• 206010034277 Pemphigoid Diseases 0.000 description 1
• BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
• 102100040682 Platelet-derived growth factor D Human genes 0.000 description 1
• 101710170209 Platelet-derived growth factor D Proteins 0.000 description 1
• 239000004698 Polyethylene Substances 0.000 description 1
• 108010039918 Polylysine Proteins 0.000 description 1
• 239000004721 Polyphenylene oxide Substances 0.000 description 1
• 239000004372 Polyvinyl alcohol Substances 0.000 description 1
• 206010036790 Productive cough Diseases 0.000 description 1
• QOSMNYMQXIVWKY-UHFFFAOYSA-N Propyl levulinate Chemical compound CCCOC(=O)CCC(C)=O QOSMNYMQXIVWKY-UHFFFAOYSA-N 0.000 description 1
• 102100024983 Protein NDNF Human genes 0.000 description 1
• 239000012980 RPMI-1640 medium Substances 0.000 description 1
• 108700008625 Reporter Genes Proteins 0.000 description 1
• 108010081750 Reticulin Proteins 0.000 description 1
• 206010057430 Retinal injury Diseases 0.000 description 1
• 206010038926 Retinopathy hypertensive Diseases 0.000 description 1
• 206010038935 Retinopathy sickle cell Diseases 0.000 description 1
• 102000006382 Ribonucleases Human genes 0.000 description 1
• 108010083644 Ribonucleases Proteins 0.000 description 1
• 108060007030 Ribulose-phosphate 3-epimerase Proteins 0.000 description 1
• 102100039270 Ribulose-phosphate 3-epimerase Human genes 0.000 description 1
• 102000012479 Serine Proteases Human genes 0.000 description 1
• 108010022999 Serine Proteases Proteins 0.000 description 1
• 102100031976 Short stature homeobox protein 2 Human genes 0.000 description 1
• UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
• DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
• 102100024471 Stabilin-1 Human genes 0.000 description 1
• 101710164042 Stabilin-1 Proteins 0.000 description 1
• 229920002472 Starch Polymers 0.000 description 1
• 229930182558 Sterol Natural products 0.000 description 1
• 206010042033 Stevens-Johnson syndrome Diseases 0.000 description 1
• 231100000168 Stevens-Johnson syndrome Toxicity 0.000 description 1
• 238000000692 Student's t-test Methods 0.000 description 1
• 229930006000 Sucrose Natural products 0.000 description 1
• CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
• QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
• 210000001744 T-lymphocyte Anatomy 0.000 description 1
• 102100024554 Tetranectin Human genes 0.000 description 1
• 108090001109 Thermolysin Proteins 0.000 description 1
• 108010000499 Thromboplastin Proteins 0.000 description 1
• 102100030859 Tissue factor Human genes 0.000 description 1
• 102100026260 Titin Human genes 0.000 description 1
• 108091023040 Transcription factor Proteins 0.000 description 1
• 102000040945 Transcription factor Human genes 0.000 description 1
• 108010009583 Transforming Growth Factors Proteins 0.000 description 1
• 102000009618 Transforming Growth Factors Human genes 0.000 description 1
• 102000056172 Transforming growth factor beta-3 Human genes 0.000 description 1
• 108090000097 Transforming growth factor beta-3 Proteins 0.000 description 1
• 208000030886 Traumatic Brain injury Diseases 0.000 description 1
• 108091000117 Tyrosine 3-Monooxygenase Proteins 0.000 description 1
• 102000048218 Tyrosine 3-monooxygenases Human genes 0.000 description 1
• 206010054880 Vascular insufficiency Diseases 0.000 description 1
• 229920001938 Vegetable gum Polymers 0.000 description 1
• 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
• 229930003268 Vitamin C Natural products 0.000 description 1
• 229930003427 Vitamin E Natural products 0.000 description 1
• 206010049644 Williams syndrome Diseases 0.000 description 1
• 201000001305 Williams-Beuren syndrome Diseases 0.000 description 1
• 210000002593 Y chromosome Anatomy 0.000 description 1
• 102100040663 Zinc finger protein 710 Human genes 0.000 description 1
• 230000002159 abnormal effect Effects 0.000 description 1
• 230000002378 acidificating effect Effects 0.000 description 1
• 230000009692 acute damage Effects 0.000 description 1
• 239000012574 advanced DMEM Substances 0.000 description 1
• 238000012382 advanced drug delivery Methods 0.000 description 1
• 230000002411 adverse Effects 0.000 description 1
• 239000008272 agar Substances 0.000 description 1
• 238000003483 aging Methods 0.000 description 1
• 150000001335 aliphatic alkanes Chemical class 0.000 description 1
• 125000002877 alkyl aryl group Chemical group 0.000 description 1
• 238000011316 allogeneic transplantation Methods 0.000 description 1
• 108010026331 alpha-Fetoproteins Proteins 0.000 description 1
• 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
• 229940024606 amino acid Drugs 0.000 description 1
• 235000001014 amino acid Nutrition 0.000 description 1
• 150000001413 amino acids Chemical class 0.000 description 1
• 210000001691 amnion Anatomy 0.000 description 1
• 229940009444 amphotericin Drugs 0.000 description 1
• 210000004102 animal cell Anatomy 0.000 description 1
• 230000006909 anti-apoptosis Effects 0.000 description 1
• 229940124599 anti-inflammatory drug Drugs 0.000 description 1
• 230000000692 anti-sense effect Effects 0.000 description 1
• 239000003146 anticoagulant agent Substances 0.000 description 1
• 229940127219 anticoagulant drug Drugs 0.000 description 1
• 210000000612 antigen-presenting cell Anatomy 0.000 description 1
• 239000002246 antineoplastic agent Substances 0.000 description 1
• 229940041181 antineoplastic drug Drugs 0.000 description 1
• 239000012298 atmosphere Substances 0.000 description 1
• 230000003305 autocrine Effects 0.000 description 1
• 230000005784 autoimmunity Effects 0.000 description 1
• 210000003719 b-lymphocyte Anatomy 0.000 description 1
• 239000003855 balanced salt solution Substances 0.000 description 1
• 230000004888 barrier function Effects 0.000 description 1
• YBHILYKTIRIUTE-UHFFFAOYSA-N berberine Chemical compound C1=C2CC[N+]3=CC4=C(OC)C(OC)=CC=C4C=C3C2=CC2=C1OCO2 YBHILYKTIRIUTE-UHFFFAOYSA-N 0.000 description 1
• 229940093265 berberine Drugs 0.000 description 1
• QISXPYZVZJBNDM-UHFFFAOYSA-N berberine Natural products COc1ccc2C=C3N(Cc2c1OC)C=Cc4cc5OCOc5cc34 QISXPYZVZJBNDM-UHFFFAOYSA-N 0.000 description 1
• WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
• 229920000249 biocompatible polymer Polymers 0.000 description 1
• 230000033228 biological regulation Effects 0.000 description 1
• 230000005540 biological transmission Effects 0.000 description 1
• 229940126587 biotherapeutics Drugs 0.000 description 1
• 210000002459 blastocyst Anatomy 0.000 description 1
• 230000000903 blocking effect Effects 0.000 description 1
• 210000000601 blood cell Anatomy 0.000 description 1
• 210000001772 blood platelet Anatomy 0.000 description 1
• 210000004204 blood vessel Anatomy 0.000 description 1
• 210000001185 bone marrow Anatomy 0.000 description 1
• 239000012888 bovine serum Substances 0.000 description 1
• 210000004556 brain Anatomy 0.000 description 1
• 239000007975 buffered saline Substances 0.000 description 1
• 239000006172 buffering agent Substances 0.000 description 1
• 210000005252 bulbus oculi Anatomy 0.000 description 1
• 239000002775 capsule Substances 0.000 description 1
• 150000001720 carbohydrates Chemical class 0.000 description 1
• 235000014633 carbohydrates Nutrition 0.000 description 1
• 125000004432 carbon atom Chemical group C* 0.000 description 1
• 239000001569 carbon dioxide Substances 0.000 description 1
• 229910002092 carbon dioxide Inorganic materials 0.000 description 1
• 238000009960 carding Methods 0.000 description 1
• 208000006170 carotid stenosis Diseases 0.000 description 1
• 230000015556 catabolic process Effects 0.000 description 1
• 229940105657 catalase Drugs 0.000 description 1
• 238000012832 cell culture technique Methods 0.000 description 1
• 230000005779 cell damage Effects 0.000 description 1
• 230000004663 cell proliferation Effects 0.000 description 1
• 230000008614 cellular interaction Effects 0.000 description 1
• 201000005667 central retinal vein occlusion Diseases 0.000 description 1
• 230000002490 cerebral effect Effects 0.000 description 1
• 230000008859 change Effects 0.000 description 1
• 238000012512 characterization method Methods 0.000 description 1
• 238000010382 chemical cross-linking Methods 0.000 description 1
• 238000006243 chemical reaction Methods 0.000 description 1
• 238000002512 chemotherapy Methods 0.000 description 1
• 229960004926 chlorobutanol Drugs 0.000 description 1
• 235000012000 cholesterol Nutrition 0.000 description 1
• 229940094517 chondroitin 4-sulfate Drugs 0.000 description 1
• KXKPYJOVDUMHGS-OSRGNVMNSA-N chondroitin sulfate Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](OS(O)(=O)=O)[C@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](C(O)=O)O1 KXKPYJOVDUMHGS-OSRGNVMNSA-N 0.000 description 1
• 210000000349 chromosome Anatomy 0.000 description 1
• 229960002376 chymotrypsin Drugs 0.000 description 1
• 238000010367 cloning Methods 0.000 description 1
• 238000007820 coagulation assay Methods 0.000 description 1
• 239000003086 colorant Substances 0.000 description 1
• 238000004891 communication Methods 0.000 description 1
• 239000003184 complementary RNA Substances 0.000 description 1
• 239000002131 composite material Substances 0.000 description 1
• 238000003271 compound fluorescence assay Methods 0.000 description 1
• 238000007906 compression Methods 0.000 description 1
• 230000006835 compression Effects 0.000 description 1
• 238000010276 construction Methods 0.000 description 1
• 238000011109 contamination Methods 0.000 description 1
• 238000013270 controlled release Methods 0.000 description 1
• 230000001276 controlling effect Effects 0.000 description 1
• 210000004351 coronary vessel Anatomy 0.000 description 1
• 239000006071 cream Substances 0.000 description 1
• 238000002788 crimping Methods 0.000 description 1
• 238000004132 cross linking Methods 0.000 description 1
• 230000002338 cryopreservative effect Effects 0.000 description 1
• 238000011461 current therapy Methods 0.000 description 1
• 238000005520 cutting process Methods 0.000 description 1
• UFULAYFCSOUIOV-UHFFFAOYSA-N cysteamine Chemical compound NCCS UFULAYFCSOUIOV-UHFFFAOYSA-N 0.000 description 1
• 238000004163 cytometry Methods 0.000 description 1
• 230000001086 cytosolic effect Effects 0.000 description 1
• 231100000433 cytotoxic Toxicity 0.000 description 1
• 230000001472 cytotoxic effect Effects 0.000 description 1
• 229920006237 degradable polymer Polymers 0.000 description 1
• 238000006731 degradation reaction Methods 0.000 description 1
• 230000000593 degrading effect Effects 0.000 description 1
• 238000006356 dehydrogenation reaction Methods 0.000 description 1
• 239000012649 demethylating agent Substances 0.000 description 1
• AVJBPWGFOQAPRH-FWMKGIEWSA-L dermatan sulfate Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@H](OS([O-])(=O)=O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](C([O-])=O)O1 AVJBPWGFOQAPRH-FWMKGIEWSA-L 0.000 description 1
• 229940051593 dermatan sulfate Drugs 0.000 description 1
• 238000003795 desorption Methods 0.000 description 1
• 230000006866 deterioration Effects 0.000 description 1
• 206010012601 diabetes mellitus Diseases 0.000 description 1
• 235000014113 dietary fatty acids Nutrition 0.000 description 1
• 235000019621 digestibility Nutrition 0.000 description 1
• 238000010790 dilution Methods 0.000 description 1
• 239000012895 dilution Substances 0.000 description 1
• 231100000673 dose–response relationship Toxicity 0.000 description 1
• 238000007878 drug screening assay Methods 0.000 description 1
• 210000003981 ectoderm Anatomy 0.000 description 1
• 208000002169 ectodermal dysplasia Diseases 0.000 description 1
• 208000031068 ectodermal dysplasia syndrome Diseases 0.000 description 1
• 101150097231 eg gene Proteins 0.000 description 1
• 238000004520 electroporation Methods 0.000 description 1
• 239000003995 emulsifying agent Substances 0.000 description 1
• 238000005538 encapsulation Methods 0.000 description 1
• 230000002124 endocrine Effects 0.000 description 1
• 210000001900 endoderm Anatomy 0.000 description 1
• 230000007613 environmental effect Effects 0.000 description 1
• 230000006862 enzymatic digestion Effects 0.000 description 1
• 239000003797 essential amino acid Substances 0.000 description 1
• 235000020776 essential amino acid Nutrition 0.000 description 1
• 235000019441 ethanol Nutrition 0.000 description 1
• 230000007717 exclusion Effects 0.000 description 1
• 238000013401 experimental design Methods 0.000 description 1
• 210000000646 extraembryonic cell Anatomy 0.000 description 1
• 210000002219 extraembryonic membrane Anatomy 0.000 description 1
• 208000030533 eye disease Diseases 0.000 description 1
• 230000004438 eyesight Effects 0.000 description 1
• 239000000194 fatty acid Substances 0.000 description 1
• 229930195729 fatty acid Natural products 0.000 description 1
• 230000004720 fertilization Effects 0.000 description 1
• 210000004700 fetal blood Anatomy 0.000 description 1
• 210000000604 fetal stem cell Anatomy 0.000 description 1
• 239000012467 final product Substances 0.000 description 1
• 239000010419 fine particle Substances 0.000 description 1
• 238000007667 floating Methods 0.000 description 1
• GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
• 238000000799 fluorescence microscopy Methods 0.000 description 1
• 238000007710 freezing Methods 0.000 description 1
• 230000008014 freezing Effects 0.000 description 1
• 229960003692 gamma aminobutyric acid Drugs 0.000 description 1
• BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 1
• WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
• 210000000609 ganglia Anatomy 0.000 description 1
• 238000001641 gel filtration chromatography Methods 0.000 description 1
• 230000030279 gene silencing Effects 0.000 description 1
• 238000012226 gene silencing method Methods 0.000 description 1
• 238000001415 gene therapy Methods 0.000 description 1
• 229960002518 gentamicin Drugs 0.000 description 1
• 239000012362 glacial acetic acid Substances 0.000 description 1
• 230000002518 glial effect Effects 0.000 description 1
• 210000005046 glial fibrillary acidic protein Anatomy 0.000 description 1
• 239000003292 glue Substances 0.000 description 1
• 239000004220 glutamic acid Substances 0.000 description 1
• 230000036252 glycation Effects 0.000 description 1
• 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
• 235000011187 glycerol Nutrition 0.000 description 1
• 230000013595 glycosylation Effects 0.000 description 1
• 238000006206 glycosylation reaction Methods 0.000 description 1
• 230000005484 gravity Effects 0.000 description 1
• RQFCJASXJCIDSX-UUOKFMHZSA-N guanosine 5'-monophosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O RQFCJASXJCIDSX-UUOKFMHZSA-N 0.000 description 1
• 229910052736 halogen Inorganic materials 0.000 description 1
• 210000003128 head Anatomy 0.000 description 1
• 229920000669 heparin Polymers 0.000 description 1
• 229960002897 heparin Drugs 0.000 description 1
• 210000003494 hepatocyte Anatomy 0.000 description 1
• 238000004128 high performance liquid chromatography Methods 0.000 description 1
• 230000006801 homologous recombination Effects 0.000 description 1
• 238000002744 homologous recombination Methods 0.000 description 1
• 102000052611 human IL6 Human genes 0.000 description 1
• 235000019447 hydroxyethyl cellulose Nutrition 0.000 description 1
• 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
• 239000001863 hydroxypropyl cellulose Substances 0.000 description 1
• 235000010977 hydroxypropyl cellulose Nutrition 0.000 description 1
• 239000001866 hydroxypropyl methyl cellulose Substances 0.000 description 1
• 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 description 1
• 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 description 1
• 201000001948 hypertensive retinopathy Diseases 0.000 description 1
• 210000002865 immune cell Anatomy 0.000 description 1
• 230000001900 immune effect Effects 0.000 description 1
• 230000003053 immunization Effects 0.000 description 1
• 238000002649 immunization Methods 0.000 description 1
• 238000003365 immunocytochemistry Methods 0.000 description 1
• 230000005847 immunogenicity Effects 0.000 description 1
• 238000003364 immunohistochemistry Methods 0.000 description 1
• 230000002480 immunoprotective effect Effects 0.000 description 1
• 229960003444 immunosuppressant agent Drugs 0.000 description 1
• 229940125721 immunosuppressive agent Drugs 0.000 description 1
• 230000001976 improved effect Effects 0.000 description 1
• 230000006872 improvement Effects 0.000 description 1
• 238000007901 in situ hybridization Methods 0.000 description 1
• 230000000415 inactivating effect Effects 0.000 description 1
• 230000002779 inactivation Effects 0.000 description 1
• 210000004263 induced pluripotent stem cell Anatomy 0.000 description 1
• 208000015181 infectious disease Diseases 0.000 description 1
• 230000004054 inflammatory process Effects 0.000 description 1
• 230000002401 inhibitory effect Effects 0.000 description 1
• 230000000977 initiatory effect Effects 0.000 description 1
• 229940102223 injectable solution Drugs 0.000 description 1
• 230000010354 integration Effects 0.000 description 1
• 229940100601 interleukin-6 Drugs 0.000 description 1
• 229940047122 interleukins Drugs 0.000 description 1
• 230000003834 intracellular effect Effects 0.000 description 1
• 238000004255 ion exchange chromatography Methods 0.000 description 1
• 150000002500 ions Chemical class 0.000 description 1
• 210000002735 iris pigment epithelial cell Anatomy 0.000 description 1
• 229910052742 iron Inorganic materials 0.000 description 1
• 230000007794 irritation Effects 0.000 description 1
• 231100001032 irritation of the eye Toxicity 0.000 description 1
• 206010023332 keratitis Diseases 0.000 description 1
• 206010023365 keratopathy Diseases 0.000 description 1
• 230000002147 killing effect Effects 0.000 description 1
• 239000004310 lactic acid Substances 0.000 description 1
• 235000014655 lactic acid Nutrition 0.000 description 1
• 239000008101 lactose Substances 0.000 description 1
• 239000002523 lectin Substances 0.000 description 1
• 210000000265 leukocyte Anatomy 0.000 description 1
• 230000000670 limiting effect Effects 0.000 description 1
• 238000011670 long-evans rat Methods 0.000 description 1
• 208000018769 loss of vision Diseases 0.000 description 1
• 231100000864 loss of vision Toxicity 0.000 description 1
• 239000000314 lubricant Substances 0.000 description 1
• 230000002934 lysing effect Effects 0.000 description 1
• 229920002521 macromolecule Polymers 0.000 description 1
• 210000002540 macrophage Anatomy 0.000 description 1
• 238000002595 magnetic resonance imaging Methods 0.000 description 1
• 238000013411 master cell bank Methods 0.000 description 1
• 238000005259 measurement Methods 0.000 description 1
• 230000001404 mediated effect Effects 0.000 description 1
• 210000003716 mesoderm Anatomy 0.000 description 1
• 239000002207 metabolite Substances 0.000 description 1
• 229920000609 methyl cellulose Polymers 0.000 description 1
• 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 1
• 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 1
• 239000001923 methylcellulose Substances 0.000 description 1
• 235000010981 methylcellulose Nutrition 0.000 description 1
• LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 1
• 238000000386 microscopy Methods 0.000 description 1
• 239000004005 microsphere Substances 0.000 description 1
• 238000007392 microtiter assay Methods 0.000 description 1
• 230000005012 migration Effects 0.000 description 1
• 238000013508 migration Methods 0.000 description 1
• 230000011278 mitosis Effects 0.000 description 1
• 238000002156 mixing Methods 0.000 description 1
• 230000004048 modification Effects 0.000 description 1
• 238000012986 modification Methods 0.000 description 1
• 210000000327 mueller cell Anatomy 0.000 description 1
• 210000000663 muscle cell Anatomy 0.000 description 1
• VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 1
• 229960004927 neomycin Drugs 0.000 description 1
• 210000000653 nervous system Anatomy 0.000 description 1
• 210000003061 neural cell Anatomy 0.000 description 1
• 210000004498 neuroglial cell Anatomy 0.000 description 1
• 230000003018 neuroregenerative effect Effects 0.000 description 1
• 239000002858 neurotransmitter agent Substances 0.000 description 1
• 229910052757 nitrogen Inorganic materials 0.000 description 1
• 235000015097 nutrients Nutrition 0.000 description 1
• 230000035764 nutrition Effects 0.000 description 1
• 125000005447 octyloxy group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])O* 0.000 description 1
• 239000003921 oil Substances 0.000 description 1
• 235000019198 oils Nutrition 0.000 description 1
• 239000006195 ophthalmic dosage form Substances 0.000 description 1
• 229940023490 ophthalmic product Drugs 0.000 description 1
• 208000020911 optic nerve disease Diseases 0.000 description 1
• 230000008520 organization Effects 0.000 description 1
• 230000036407 pain Effects 0.000 description 1
• 229940055729 papain Drugs 0.000 description 1
• 235000019834 papain Nutrition 0.000 description 1
• 244000052769 pathogen Species 0.000 description 1
• 230000035515 penetration Effects 0.000 description 1
• 229940056360 penicillin g Drugs 0.000 description 1
• 230000000737 periodic effect Effects 0.000 description 1
• 230000002093 peripheral effect Effects 0.000 description 1
• 210000001428 peripheral nervous system Anatomy 0.000 description 1
• 230000035699 permeability Effects 0.000 description 1
• 210000000680 phagosome Anatomy 0.000 description 1
• 239000008177 pharmaceutical agent Substances 0.000 description 1
• 239000000546 pharmaceutical excipient Substances 0.000 description 1
• 238000002135 phase contrast microscopy Methods 0.000 description 1
• 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
• JOVOSQBPPZZESK-UHFFFAOYSA-N phenylhydrazine hydrochloride Chemical compound Cl.NNC1=CC=CC=C1 JOVOSQBPPZZESK-UHFFFAOYSA-N 0.000 description 1
• 229940096826 phenylmercuric acetate Drugs 0.000 description 1
• PDTFCHSETJBPTR-UHFFFAOYSA-N phenylmercuric nitrate Chemical compound [O-][N+](=O)O[Hg]C1=CC=CC=C1 PDTFCHSETJBPTR-UHFFFAOYSA-N 0.000 description 1
• 102000036213 phospholipid binding proteins Human genes 0.000 description 1
• 108091011000 phospholipid binding proteins Proteins 0.000 description 1
• 210000004991 placental stem cell Anatomy 0.000 description 1
• 229920003023 plastic Polymers 0.000 description 1
• 239000004033 plastic Substances 0.000 description 1
• 230000008488 polyadenylation Effects 0.000 description 1
• 229920000570 polyether Polymers 0.000 description 1
• 229920000573 polyethylene Polymers 0.000 description 1
• 229920000656 polylysine Polymers 0.000 description 1
• 229920000136 polysorbate Polymers 0.000 description 1
• 229950008882 polysorbate Drugs 0.000 description 1
• 229940068977 polysorbate 20 Drugs 0.000 description 1
• 229920002451 polyvinyl alcohol Polymers 0.000 description 1
• 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
• 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
• 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
• 239000011148 porous material Substances 0.000 description 1
• 230000008092 positive effect Effects 0.000 description 1
• 238000012809 post-inoculation Methods 0.000 description 1
• 230000002335 preservative effect Effects 0.000 description 1
• 230000002265 prevention Effects 0.000 description 1
• 239000000651 prodrug Substances 0.000 description 1
• 229940002612 prodrug Drugs 0.000 description 1
• 238000003498 protein array Methods 0.000 description 1
• 230000006920 protein precipitation Effects 0.000 description 1
• 101150036383 rad16 gene Proteins 0.000 description 1
• 239000000700 radioactive tracer Substances 0.000 description 1
• 238000003753 real-time PCR Methods 0.000 description 1
• 230000009467 reduction Effects 0.000 description 1
• 102000037983 regulatory factors Human genes 0.000 description 1
• 108091008025 regulatory factors Proteins 0.000 description 1
• 230000001846 repelling effect Effects 0.000 description 1
• 230000008943 replicative senescence Effects 0.000 description 1
• 230000004044 response Effects 0.000 description 1
• 238000010993 response surface methodology Methods 0.000 description 1
• 230000000284 resting effect Effects 0.000 description 1
• 230000000717 retained effect Effects 0.000 description 1
• 230000004243 retinal function Effects 0.000 description 1
• 210000000844 retinal pigment epithelial cell Anatomy 0.000 description 1
• 208000004644 retinal vein occlusion Diseases 0.000 description 1
• 230000001177 retroviral effect Effects 0.000 description 1
• 238000012340 reverse transcriptase PCR Methods 0.000 description 1
• 239000012487 rinsing solution Substances 0.000 description 1
• 239000012266 salt solution Substances 0.000 description 1
• 210000004116 schwann cell Anatomy 0.000 description 1
• 210000003786 sclera Anatomy 0.000 description 1
• 239000006152 selective media Substances 0.000 description 1
• 238000010008 shearing Methods 0.000 description 1
• 239000002356 single layer Substances 0.000 description 1
• 230000000391 smoking effect Effects 0.000 description 1
• HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 description 1
• 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
• 229940001584 sodium metabisulfite Drugs 0.000 description 1
• 235000010262 sodium metabisulphite Nutrition 0.000 description 1
• 229940054269 sodium pyruvate Drugs 0.000 description 1
• 230000003381 solubilizing effect Effects 0.000 description 1
• 230000000920 spermatogeneic effect Effects 0.000 description 1
• 210000003802 sputum Anatomy 0.000 description 1
• 208000024794 sputum Diseases 0.000 description 1
• 239000003381 stabilizer Substances 0.000 description 1
• 230000000087 stabilizing effect Effects 0.000 description 1
• 239000008107 starch Substances 0.000 description 1
• 235000019698 starch Nutrition 0.000 description 1
• 239000008223 sterile water Substances 0.000 description 1
• 150000003432 sterols Chemical class 0.000 description 1
• 235000003702 sterols Nutrition 0.000 description 1
• 230000000638 stimulation Effects 0.000 description 1
• 229960002385 streptomycin sulfate Drugs 0.000 description 1
• 230000035882 stress Effects 0.000 description 1
• 210000003537 structural cell Anatomy 0.000 description 1
• 238000013337 sub-cultivation Methods 0.000 description 1
• 239000005720 sucrose Substances 0.000 description 1
• 235000000346 sugar Nutrition 0.000 description 1
• LSNNMFCWUKXFEE-UHFFFAOYSA-L sulfite Chemical class [O-]S([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-L 0.000 description 1
• 239000004094 surface-active agent Substances 0.000 description 1
• 238000013268 sustained release Methods 0.000 description 1
• 239000012730 sustained-release form Substances 0.000 description 1
• 230000009885 systemic effect Effects 0.000 description 1
• 239000003826 tablet Substances 0.000 description 1
• 125000003831 tetrazolyl group Chemical group 0.000 description 1
• 239000004753 textile Substances 0.000 description 1
• 238000010257 thawing Methods 0.000 description 1
• 238000009210 therapy by ultrasound Methods 0.000 description 1
• RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
• 229940033663 thimerosal Drugs 0.000 description 1
• 230000008467 tissue growth Effects 0.000 description 1
• 239000002407 tissue scaffold Substances 0.000 description 1
• 239000012929 tonicity agent Substances 0.000 description 1
• 231100000167 toxic agent Toxicity 0.000 description 1
• 239000003440 toxic substance Substances 0.000 description 1
• 239000003053 toxin Substances 0.000 description 1
• 231100000765 toxin Toxicity 0.000 description 1
• 238000013518 transcription Methods 0.000 description 1
• 230000035897 transcription Effects 0.000 description 1
• 239000012096 transfection reagent Substances 0.000 description 1
• 229940072041 transforming growth factor beta 2 Drugs 0.000 description 1
• 230000010474 transient expression Effects 0.000 description 1
• 230000005945 translocation Effects 0.000 description 1
• 230000008733 trauma Effects 0.000 description 1
• 238000011277 treatment modality Methods 0.000 description 1
• GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
• 229910052721 tungsten Inorganic materials 0.000 description 1
• 239000010937 tungsten Substances 0.000 description 1
• 238000007492 two-way ANOVA Methods 0.000 description 1
• 108010002164 tyrosine receptor Proteins 0.000 description 1
• 210000003606 umbilical vein Anatomy 0.000 description 1
• 210000001113 umbilicus Anatomy 0.000 description 1
• 208000023577 vascular insufficiency disease Diseases 0.000 description 1
• 239000003981 vehicle Substances 0.000 description 1
• 230000003612 virological effect Effects 0.000 description 1
• 230000004304 visual acuity Effects 0.000 description 1
• 229940046009 vitamin E Drugs 0.000 description 1
• 108010047303 von Willebrand Factor Proteins 0.000 description 1
• 102100036537 von Willebrand factor Human genes 0.000 description 1
• 229960001134 von willebrand factor Drugs 0.000 description 1
• PJVWKTKQMONHTI-UHFFFAOYSA-N warfarin Chemical compound OC=1C2=CC=CC=C2OC(=O)C=1C(CC(=O)C)C1=CC=CC=C1 PJVWKTKQMONHTI-UHFFFAOYSA-N 0.000 description 1
• 239000011534 wash buffer Substances 0.000 description 1
• 239000000080 wetting agent Substances 0.000 description 1
• 210000001325 yolk sac Anatomy 0.000 description 1