TW201519798A - A composition and a method for manufacturing the composition thereof - Google Patents

Abstract

A composition with anti-septic and insect-resistant properties is disclosed. The composition comprises eucommia, cinnamon, citronella, verbena, citrus and a solvent, wherein a weight-to-volume ratio of eucommia is 0.05-5%, wherein a weight-to-volume ratio of cinnamon is 0.05-5%, wherein a weight-to-volume ratio of citronella is 0.05-5%, wherein a weight-to-volume ratio of verbena is 0.05-5%, wherein a weight-to-volume ratio of citrus is 0.05-5%.

Description

Compound extract and preparation method thereof

The present invention relates to a compound extract, in particular to a compound extract derived from natural plants and having antiseptic and insecticidal effects, and to a method for preparing the compound extract.

Since ancient times, skin care products have been waiting for people to expect better skin conditions. In order to avoid the maintenance of products containing abundant carbon and nitrogen sources as a breeding ground for bacterial reproduction, the deterioration of skin care products and the use of preservatives in skin care products to slow down the growth and reproduction of microorganisms are necessary means; therefore, many chemical antiseptics The agent was born in response.

In the modern industry where the chemical industry is developed, many conventional chemical preservatives have been able to be mass-produced to reduce costs, making the conventional chemical preservatives abundant in various inexpensive or even expensive skin care products. The conventional chemical preservative generally has a good antiseptic effect and can prolong the shelf life of the skin care product to several years. However, the conventional chemical preservative has many skin or body problems, such as a Methylisothiazolinone is prone to skin irritation, rash or blisters. In addition to aggravating atopic dermatitis and eczema, it also has a risk of cellular and neurotoxicity; methylparaben is in When coexisting with amino acid for a long time, it is easy to release formaldehyde which is irritating to the skin; triclosan is more irritating to the skin, and is more likely to react with water containing too much chlorine to produce chloroform. Carcinogens.

On the other hand, some natural skin care products are caused by the reduction of conventional chemical preservatives. Allergic, choose not to add the conventional chemical preservative, although it is produced under complete sterility in the process, the antiseptic effect of the natural skin care product can only last for several days to several weeks, not only the storage period is short, but also after opening. Caution will cause serious microbial contamination and damage to the skin.

Accordingly, if a compound extract having an antiseptic function is provided, which comprises extracting an effective natural preservative component from a plant, and obtaining the compound extract in a simple step, the skin care product or the pharmaceutical product can be provided. And the preservatives used in foods are more secure and convenient to choose.

The main object of the present invention is to provide a compound extract which is derived from natural plants and which is used for preservation and pest control.

Still another object of the present invention is to provide a method for preparing a compound extract obtained by extracting a compound extract and completing the preparation of the compound extract in a simple step.

In order to achieve the foregoing object, the technical means and the effects achievable by the technical method include: a compound extract comprising 0.05 to 5% by weight and volume percentage of Eucommia extract, 0.05 ~5% of cinnamon extract, 0.05~5% of citronella extract, 0.05~5% of verbena extract and 0.05~5% of citrus extract, the rest of which is a solvent.

A compound extract of the present invention, wherein the compound extract preferably comprises 1% by weight of eucommia extract, 1% of cinnamon extract, 1% of citronella extract, 1% of whip The grass extract and 1% citrus extract are the rest of the solvent.

A compound extract of the present invention, wherein the solvent is preferably selected from the group consisting of water, alcohols, ketones, or supercritical carbon dioxide fluids.

A compound extract of the present invention, wherein the citrus plant is preferably a mandarin Orange or Lyme.

A method for preparing a compound extract, comprising: providing a mixture comprising Eucommia, Cinnamon, Lemongrass, Verbena and Citrus; providing a solvent; 1/400 to 1/4 by weight to volume Mixing the mixture with the solvent in a ratio; and extracting the mixture with the solvent to obtain a compound extract, wherein the compound extract contains 0.05 to 5% by weight of the Eucommia extract, 0.05~ 5% of cinnamon extract, 0.05-5% of citronella extract, 0.05-5% of verbena extract and 0.05-5% of citrus extract, and the rest of the ingredients are the solvent.

The method for producing a compound extract of the present invention, wherein the mixture is preferably mixed with the solvent in a ratio of 1/20 by weight to volume.

The preparation method of the compound extract of the present invention, wherein the solvent is water, and the mixture is further heated to 80 to 121 ° C when the mixture is extracted with the solvent.

In the method for preparing a compound extract of the present invention, it is preferred to further filter the compound extract by a filtration membrane.

In the preparation method of the compound extract of the present invention, the average pore diameter of the filtration membrane is preferably 0.22 μm.

The preparation method of the compound extract of the present invention, wherein preferably, the compound extract is allowed to stand for 4 to 18 hours, and the particulate impurities of the compound extract are precipitated to facilitate filtration.

The compound extract of the present invention achieves antiseptic and insect-proof properties and is not susceptible to sensitization by combining natural components of various plant extracts.

The preparation method of the compound extract of the invention directly extracts the natural antiseptic component from the plant, and achieves the effect of obtaining the compound extract.

Figure 1a: Photograph of the collagen solution in the container of Group C0 on the 540th day of the experiment.

Figure 1b: Photograph of the bottle No. of Group C0 on the 540th day of the experiment.

Figure 1c: Photograph of the collagen solution in the C1 group container of the experiment on the 540th day.

Figure 1d: Photograph of the bottle mouth of Group C1 of this experiment on the 540th day.

Figure 2a: Photograph of the first day of the fish in the E0 group of this experiment.

Figure 2b: Photograph of the E0 group of pork slices on the fourth day of the experiment.

Figure 2c: Photograph of the first day of the fish in the E1 group of this experiment.

Figure 2d: Photograph of the E1 group of pork slices on the fourth day of the experiment.

The above and other objects, features and advantages of the present invention will become more <RTIgt; The extract comprises 0.05 to 5% by weight of eucommia extract, 0.05 to 5% of cinnamon extract, 0.05 to 5% of lemongrass extract, 0.05 to 5% of verbena extract and 0.05~ 5% of the citrus extract, the remaining components are a solvent; preferably, the compound extract may comprise 1% by weight of eucommia extract, 1% of cinnamon extract, 1% Lemongrass extract, 1% verbena extract and 1% citrus extract, and the remaining ingredients are the solvent.

In detail, the Eucommia extract of the preferred embodiment is extracted from the bark, root or fruit of Eucommia ulmoides, preferably extracted from the bark of Eucommia ulmoides, so as to obtain gutta-percha, pectin, alkaloid, sugar.有效 and other active ingredients.

In the preferred embodiment, the bark, branches, leaves or fruits of cinnamon are used, and preferably the bark of cinnamon is used. The cinnamon contains active ingredients such as cinnamaldehyde, cinnamic acid, cinnamic ester, eugenol and enamel.

In the preferred embodiment, the leaves, flowers or whole plants of the lemongrass are selected, preferably the leaves of the lemongrass are selected, wherein the lemongrass comprises effective citrate, citronellal, myrcene and glucosinolates. Minute.

In the preferred embodiment, the leaves, flowers or whole plants of verbena are used, preferably the leaves of verbena are used, wherein the verbena contains active ingredients such as β-sitosterol and verbena.

The citrus plant comprises Lyme and citrus, etc., and the preferred embodiment is obtained from the leaves, peels or bark of Lyme or Citrus, preferably citrus leaves, wherein the citrus plant comprises alkaloids and coumarins. Active ingredients such as flavonoids and terpenoids.

In addition, the solvent of the preferred embodiment may be one of water, alcohol, ketone or supercritical carbon dioxide fluid, and the purpose is to extract the components in the plant, which can be understood by those having ordinary knowledge in the field. Repeat them.

In order to obtain the compound extract of the preferred embodiment, the preparation method of the compound extract generally comprises the steps of: providing a mixture, providing a solvent, mixing the mixture with the solvent, and extracting the mixture with the solvent to A compound extract is obtained.

In detail, a mixture is provided which comprises Eucommia, Cinnamon, Lemongrass, Verbena and Citrus, which are respectively mixed after weighing the appropriate dry weight; wherein the citrus is citrus or Lyme. In the preferred embodiment, both Eucommia and Cinnamon use bark portions, citronella and verbena are leaves, and citrus plants use citrus leaves or lime leaves.

The solvent is provided, and the solvent may be selected from water, alcohol, ketone or supercritical carbon dioxide fluid for the purpose of extracting the active ingredient in the plant, which is understood by those skilled in the art and will not be described. In the preferred embodiment, water is used as the solvent, so that the compound extract of the preferred embodiment does not cause skin irritation due to the solvent, and can be directly consumed, and thus can be used as an additive for pharmaceuticals or health foods.

Next, the mixture and the solvent are mixed, and the mixture and the solvent are preferably mixed at a ratio of 1/400 to 1/4 by weight to volume to achieve an effective concentration of the extracted compound extract, and the cost is not wasted. . In detail, weighed eucommia, cinnamon, lemongrass, verbena and citrus plants with a dry weight of 0.5~50g, and mixed with 1000ml of water. Extracting to obtain the compound extract; at this time, the compound extract contains 0.05 to 5% Eucommia extract, cinnamon extract, lemongrass extract, verbena extract and citrus plants in a weight-to-volume ratio The extract is the rest of the water.

The compound extract of the invention can be obtained by weighing the dry weight of Eucommia ulmoides, cinnamon, lemongrass, verbena and citrus plants, mixing them with water, extracting them by water, or weighing eucommia, cinnamon, lemongrass, verbena After the dry weight of the citrus plant, water is separately added and the extract of Eucommia ulmoides, the extract of cinnamon, the extract of citronella, the extract of verbena and the extract of citrus are separately extracted, and then the extracts of the unilateral are mixed; Separate extraction does not affect the antiseptic and insecticidal effects of the compound extract of the invention.

In the preferred embodiment, 5 g of Eucommia, Cinnamon, Lemongrass, Verbena and Citrus are weighed separately so that the total weight is 25 g of the mixture, and the ratio is 1/20 by weight to volume. The mixture was mixed with 500 ml of water for extraction.

In addition, in order to increase the speed of extraction to save the process time, in the preferred embodiment, when the mixture is extracted by solvent, another water extraction is carried out at 80-100 ° C, or at 121 ° C at 1.5 atm. Extraction to obtain a heated extract; the heated compound extract can be cooled at room temperature or in a refrigerating device, or cooled with water to facilitate subsequent processing.

Preferably, after the mixture is mixed with the solvent for extraction, in order to remove the residue left in the extraction process of the compound extract to increase the quality and storage time of the compound extract, the compound extract may have an average pore diameter of Filter through a 0.22 μm filter membrane. Before filtering, the sieve can be used to remove the water-insoluble mixture raw material, and the larger residue can be removed. The compound extract can be centrifuged for 5 minutes at 3000 rpm for preliminary centrifugation to precipitate a larger residue at the bottom. A supernatant is obtained; the compound extract may be firstly filtered with one to several filtration membranes having a larger average pore diameter (for example, 0.45 μm to 5 mm) to obtain a crude filtrate. After the initial centrifugation or preliminary filtration, the supernatant or the larger residue of the coarse filtrate has already Since it is removed, in the process of filtering the supernatant and the crude filtrate through a filtration membrane having an average pore diameter of 0.22 μm, the filtration time can be increased, and the consumption of the filtration membrane can be reduced to save costs.

If there is no limitation of the processing time, after the mixture is mixed with the solvent for extraction, the compound extract may be removed from the water-insoluble mixture raw material by a sieve, and then directly left to stand and the residue is precipitated by gravity to obtain a After standing, the supernatant liquid is filtered, and the supernatant liquid is filtered without using an average membrane pore size of 0.22 μm, which can save the cost of using the filter membrane; the rest process can be carried out at room temperature or in a refrigerating apparatus, preferably. In order to stand for 4 to 18 hours, the particulate impurities are precipitated. After the standing, the supernatant may be further filtered at 0.22 μm or heated at 90 ° C for 2 hours for heat sterilization.

In order to prove that the compound extract of the present invention does have a preservative effect, and verify that the compound extract can exert a preservative effect ratio, a collagen solution is used as a representative sample of the general care product, and the container is used to hold the collagen solution. And the extract was subjected to the following experiment. The container in the experiment is preferably sterilized by radiation, autoclaving or ethanol wiping, which is understood by those skilled in the art and will not be described.

( A ) Antibacterial effect test of each extract in a single open container

First, in order to verify the bacteriostasis effect of each extract, 5g of eucommia, cinnamon, lemongrass, verbena and citrus leaves were respectively put into 100ml of water, at which time the weight of each component and water The volume ratio is 1/20, and the mixture is heated to boiling for at least 30 minutes, and after cooling, the residue is filtered to obtain a single solution; each of the solutions is adjusted with water to adjust the concentration of the extract to 2.5 g/100 ml (at this time by weight) The volume ratio is 2.5%) to obtain a single extract, including Eucommia extract, cinnamon extract, lemongrass extract, verbena extract and citrus leaf extract.

In addition to the single extracts, prepare a concentration of 0.5g / 100ml collagen solution (in this case, 0.5% by weight), 500ml of Eucommia extract, cinnamon extract, lemongrass extract, whip Grass extract and citrus leaf extract mixed with 500ml collagen The solution is divided into different groups of experimental groups (please refer to Table 1, Groups A1 to A5), so that the final concentration of various compound extracts is 2.5% by weight, and 1000 ml of 1% collagen solution with 500 ml of water is added. As a control group (Group A0), the final concentration of the collagen solution was 0.5% by weight, contained in a sterilized container, which was an uncovered open container, so that microorganisms in the air naturally fell into the container. Then, the date of the collagen solution and each of the extracts to the container was taken as the first day, and stored at room temperature, and the number of days in which the collagen solution was observed to be microbial contaminated was taken as the storage time, and the comparison of each group was performed. result.

Please refer to Table 1 for the time of microbial contamination in the collagen solution of each group in this experiment. In the control group to which no extract was added, the microbial contamination was visible to the naked eye after 1 day of storage of the collagen solution; and in the experimental group in which each of the extracts was added, the inhibition of the collagen solution by the extract of cinnamon was observed. The bacteria work best and can extend the time to contamination to 10 days.

( B ) Antibacterial effect of different concentrations of compound extracts in open containers

In order to verify that the compound extract of the preferred embodiment has more unilateral extracts For better bacteriostatic effect, in this experiment, each of the single extracts is mixed at different volume percentages to obtain different concentrations of the compound extract, and the final volume of the compound extract at different concentrations is 500 ml, and the weight is separately mixed. The percentage of the 0.5% collagen solution is 500 ml, and the time of microbial contamination in each group is observed. Among them, the method for obtaining each of the extracts, the container for use, and the calculation method of the number of days of contamination are the same as the above-mentioned single extracts. The antibacterial effect experiment in open space is the same. In this experiment, the final concentration of each extract contained in the compound extract is divided into three groups, and the concentration of the B1 to B3 groups is referred to the second table.

Please refer to the second table for the time of microbial contamination in the collagen solution of each group. When the collagen solution is added to the compound extract containing the final concentration of each extract in a single concentration of 1.25%, 2.5% and 5% by volume, the time of microbial contamination in the collagen solution can be found, with the unilateral The final concentration of each extract was increased and extended. When the compound extract contained an extract having a final concentration of 5% by volume (i.e., Group 3), the storage days were extended to 22 days.

In combination with the above experiment, in an open container, the collagen solution without any extract can be stored for only one day. If the collagen solution of each of the extracted extracts is compared, The cinnamon extract having a weight-to-volume ratio of 2.5% has a better bacteriostatic ability and can extend the storage time to 10 days. If comparing a collagen solution containing a compound extract of a different concentration of each of the extracts, wherein the collagen solution is added with a compound extract containing 1.25% by volume of the extract, even if the concentration is only 2.5% of the cinnamon extract Half, but with a longer shelf life (14 days); when the collagen solution is added to the compound extract containing 2.5% by volume of the extract, the storage time can be extended to 17 days; The final concentration of the extract in the extract was increased to 5% by volume, and the storage time was increased to 22 days.

The above experiment confirmed that in the open container, the compound extract of the preferred embodiment has a bacteriostatic effect superior to that of the single extract. Next, in order to simulate the package state of the general care product, the following experiment was conducted by placing the compound extract and the collagen solution in a sealed container.

( C ) Antibacterial effect of compound extract in closed container

The compound extract of the preferred embodiment is mixed with 1% by weight of 100% collagen solution (containing 1% by weight of eucommia extract, 1% of cinnamon extract, 1% of citronella) 100 ml of the extract, 1% verbena extract and 1% citrus leaf extract, the final concentration of the collagen solution was 0.5%, 200 ml, and was placed in the sterilized container as an experimental group (Group C1); In the control group (Group C0), the compound extract in the experimental group was replaced with 100 ml of lemongrass extract (5% by weight). The containers of Groups C0 and C1 were sealed and stored at room temperature, and the date when the collagen solution was placed in the container was taken as the first day, and the number of days in which the collagen solution was observed to be microbial contaminated was taken as the storage time, and each group was compared. Other results are recorded in Table 3. Among them, observe the number of days until the 540th day.

Please refer to Table 3 for the minimum shelf life of each sample in the control group and the experimental group. In the sealed container, the control container 1~5 without the preservative added to the collagen solution was microbial contaminated after 3 to 4 days of storage; in contrast, the experimental group was added with the compound extract in the collagen solution. Containers 1~5 can be stored for more than 540 days.

Please refer to the pictures on the 540th day of the C0 and C1 groups as shown in Figures 1a to 1d. Without adding the preferred embodiment (Group C0), the collagen solution in the container is overgrown with microorganisms (Fig. 1a, indicated by the arrow), and the gas pressure is generated due to microbial contamination, so that the liquid in the container is squeezed. The pressure is sprayed to the mouth of the bottle (Fig. 1b, indicated by the arrow); in contrast, the addition of the collagen solution in the container of the preferred embodiment (Group C0) does not observe microbial growth (Fig. 1c), bottle There is also no liquid leakage at the mouth (Fig. 1d).

Referring to Table 3 and Figures 1a to 1d together, the compound extract of the preferred embodiment does have an antiseptic effect. In a closed container, the addition of the compound extract delays the growth of the microorganism in the collagen solution. More than a year and a half.

( D ) Antibacterial effect of compound extracts in sealed containers on different concentrations of collagen solution

Please refer to Table 4 for the experimental group conditions for the compound extracts for different concentrations of collagen solution. The collagen solution is divided into four groups, which are respectively 100 ml of different concentrations of collagen solution of 0.25%, 0.5%, 1% and 5% by weight and volume ratio, and each group of collagen solution contains the compound of the preferred embodiment. Extract (Group D1, containing 1% by weight of eucommia extract, 1% cinnamon extract, 1% citronella extract, 1% verbena extract and 1% citrus leaves) The control group); the control group (D0 group) replaces the compound extract in the group D1 with the lemongrass extract, and the containers of each group are also sealed and stored at room temperature, and the solution is filled into the container. The date was taken as the first day, and the number of days in which microbial contamination of the solution was observed was taken as the storage time, and the results of each group were compared. Among them, the observation days to the 540th day stop.

Please refer to Table 4, in the D0 group of the citronella extract, the higher the concentration of the collagen solution, the shorter the storage time, presumably because collagen can provide the nitrogen source required for microbial growth, so nitrogen The more the source, the more beneficial to microbial growth; however, when the compound extract (ie, group D1) was added to the collagen solution, no microbial contamination occurred in the collagen solution at high or low concentrations until the 540th day of the experiment. .

( E ) Antiseptic effect of the compound extract of the preferred embodiment on meat

In this experiment, the raw meat pieces were used as samples (including raw fish and pork slices, respectively), and the raw meat pieces were simply wiped with paper towels to remove excess water; the E1 group was sprayed with the compound extract of the preferred embodiment (including The weight-to-volume ratio is 1% Eucommia extract, 1% cinnamon extract, 1% lemongrass extract, 1% verbena extract and 1% citrus leaf extract), placed in a disposable bowl. Cover the plastic cover with two venting holes and place it in a room temperature environment. In the E0 group, only remove the excess water from the raw meat pieces, place them in a disposable bowl and observe at room temperature. Among them, the experimental results were qualitatively evaluated by the naked eye and the sense of smell, and the results were recorded in Table 5.

Please refer to the results in Table 5. The raw fish or pork slices in Group E0 will start to produce odor on the first day of the experiment, and on the second day, the appearance of eggs will be observed on the raw meat slices, Figures 2a and 2b. The photos of the raw fish or the pork slices of the E0 group on the 4th day are respectively displayed; otherwise, the E1 group of the compound extract of the preferred embodiment is sprayed, the raw fish is odorous on the 3rd day, and the pork slices are in the first The odor was started in 5 days, and even the pork slices could be placed on the 7th day at room temperature to observe the production of eggs. The 2c and 2d images show the raw fish or pork slices of the E1 group on the 4th day. The photograph is such that the compound extract of the preferred embodiment can also be added to the food to prevent the odor generated by the bacteria and to prevent the appearance of eggs.

In summary, the compound extract of the preferred embodiment is a combination of a plurality of plant extracts, and the natural component of the compound extract has better antiseptic effect than the single extracts, and the collagen is high and low in concentration. The protein solution can exert excellent antiseptic effect and can reduce the allergic phenomenon caused by the use of the conventional chemical preservative, so as to achieve the effect of prolonging the shelf life and not causing allergies.

Furthermore, the preparation method of the compound extract of the present invention directly extracts the natural antiseptic component in the plant by solvent to achieve the effect of obtaining the compound extract extracted by the plant.

Although the present invention has been disclosed by the above-described preferred embodiments, it is not intended to limit the invention, and it is apparent to those skilled in the art without departing from the spirit and scope of the invention. The various modifications and variations of the embodiments are still within the technical scope of the invention. The scope of the invention is defined by the scope of the appended claims.

Claims (13)

A compound extract comprising 0.05 to 5% by weight of eucommia extract, 0.05 to 5% of cinnamon extract, 0.05 to 5% of citronella extract, 0.05 to 5% of verbena extract And 0.05 to 5% of the citrus extract, the remaining components are a solvent. The compound extract according to claim 1, wherein the compound extract comprises 1% by weight of Eucommia extract, 1% of cinnamon extract, 1% of lemongrass extract, 1% of Verbena extract and 1% of citrus extract, the rest of which is the solvent. The compound extract according to claim 1 or 2, wherein the solvent is selected from the group consisting of water, alcohols, ketones or supercritical carbon dioxide fluids. The compound extract according to claim 1 or 2, wherein the citrus plant is citrus or lime. A method for preparing a compound extract, comprising: providing a mixture comprising Eucommia, Cinnamon, Lemongrass, Verbena and Citrus; providing a solvent; 1/400 to 1/4 by weight to volume Mixing the mixture with the solvent; and extracting the mixture with the solvent to obtain a compound extract; wherein the compound extract contains 0.05 to 5% by weight of the Eucommia extract, 0.05~ 5% of cinnamon extract, 0.05-5% of citronella extract, 0.05-5% of verbena extract and 0.05-5% of citrus extract, and the rest of the ingredients are the solvent. The method for preparing a compound extract according to claim 5, wherein the compound extract comprises 1% by weight of the eucommia extract, 1% Cinnamon extract, 1% citronella extract, 1% verbena extract and 1% citrus extract, the remaining ingredients are the solvent. The method for preparing a compound extract according to claim 5, wherein the solvent is selected from the group consisting of water, alcohols, ketones, or supercritical carbon dioxide fluids. The method for preparing a compound extract according to claim 5, wherein the citrus plant is citrus or lime. The method for producing a compound extract according to claim 5, wherein the mixture is mixed with the solvent in a ratio of 1/20 by weight to volume. The method for preparing a compound extract according to claim 5, wherein the solvent is water, and the mixture is heated to 80 to 121 ° C when the mixture is extracted with the solvent. The method for preparing a compound extract according to claim 5, further comprising filtering the compound extract with a filtration membrane. The method for producing a compound extract according to the invention of claim 11, wherein the filter membrane has an average pore diameter of 0.22 μm. The method for preparing a compound extract according to claim 5, wherein the compound extract is further allowed to stand for 4 to 18 hours.