JP2015096498A - Ages decomposer - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide active measures for dissolving AGEs produced by glycation reaction, which are required while a specific plant extract is reported to be effective for inhibiting protein glycation reaction as a preventive measure against protein glycation reaction.SOLUTION: The invention provides an AGEs decomposer with an action decomposing AGEs, containing one more kinds selected from extracts of plants belonging to Oleaceae, Celastraceae, Saururaceae, Eucommia ulmoides, and Poaceae; and an AGEs decomposer with an action decomposing AGEs containing one or more kinds selected from Banaba tea, Meigui tea, Rose hips tea, Jasmin tea, Chicory tea, Taraxacum tea, Chamomile tea, Burdock tea, Houttuynia cordata tea, Salacia tea, Eucommia ulmoides tea, Brown rice tea, and Blueberry tea, and the like to solve the above problem.

Description

  The present invention relates to an AGEs degrading agent having an action of degrading a final product (AGEs) by a protein saccharification reaction, and foods and drinks and pharmaceuticals containing the AGEs degrading agent.

  Protein saccharification reaction (hereinafter abbreviated as saccharification reaction) C. It became known as Maillard reaction because Maillard discovered that heating amino acids and reducing sugars produced brown pigments. The Maillard reaction may be referred to as one aspect of the saccharification reaction, but may be referred to as the Maillard reaction as a general term for the saccharification reaction. In recent years, it has been revealed that this saccharification reaction is also involved in aging, dementia, cancer, hypertension, arteriosclerosis and the like. For example, the protein turns brown due to the saccharification reaction, which causes dullness on the skin and the like. Further, the skin and bone collagen is hardened by the saccharification reaction, so that the elasticity and flexibility of the skin and bone are impaired. Thus, various studies have been conducted to inhibit saccharification reactions that have various effects on living bodies.

  FIG. 1 shows the reaction route of the saccharification reaction. Although not all of the saccharification reaction pathways have been elucidated, first, a Schiff base is formed by the reaction of a reducing sugar such as glucose with the amino group of a protein or amino acid, followed by enaminol, and then by Amadori rearrangement. It becomes a stable Amadori compound. The reaction so far is called the first stage in the saccharification reaction system.

  In the later stage following the earlier stage, the Amadori compound is dehydrated, hydrolyzed, and cleaved between carbons, resulting in two carbonyl groups in the molecule such as glyoxal (GO), methylglyoxal (MG), and 3-deoxyglucosone (3DG). An α-dicarbonyl compound having (C = 0) is produced. These products are called saccharification reaction intermediates. Thereafter, AGEs (advanced glycation end products), which are the final products of saccharification reaction, are generated by in vivo degradation of α-dicarbonyl compounds, Schiff bases and Amadori compounds, aldehydes derived from lipid peroxidation, and auto-oxidation and decomposition of sugars. To do. The name AGEs is a general term for the final product by the saccharification reaction, and is not a compound showing a certain structure.

  It has been reported that an extract of a specific plant is effective for suppressing the reaction against the saccharification reaction as described above. Although suppression of the saccharification reaction is effective as a preventive measure, on the other hand, a therapeutic (aggressive) measure of degrading AGEs generated by the saccharification reaction is also required.

Japanese Patent No. 4195840

  In view of the above circumstances, an object of the present invention is to provide an AGE degrading agent having an action of degrading AGEs produced by a protein saccharification reaction.

  As means for solving the above problems, the following inventions and the like are provided. That is, as the first invention, there is provided an AGEs decomposing agent comprising at least one selected from the extracts of plants belonging to the family Moleaceae, Euphoridae, Dokudamiaceae, Euphorbiaceae, and Gramineae, and having an action of degrading AGEs To do.

  In addition, as a second invention, AGEs are decomposed, including one or more selected from banaba, mayi, rosehip, jasmine, chicory, dandelion, chamomile, burdock, dokudami, salacia, eucommia, brown rice, and blueberry extract An AGEs decomposing agent having the action of

  In addition, as the third invention, extraction of banaba tea, mayi tea, rosehip tea, jasmine tea, chicory tea, dandelion root tea, chamomile tea, burdock tea, dokudami tea, salacia tea, eucommia tea, brown rice tea, blueberry tea Provided is an AGEs decomposing agent containing one or more selected from among substances and having an action of decomposing AGEs.

  Moreover, the food-drinks, health food, food additive, cosmetics, pharmaceutical, and quasi-drug containing the AGEs decomposition agent as described in any one of 3rd invention from 1st invention are provided.

  According to the present invention, it is possible to provide an AGE degrading agent having an action of degrading AGEs produced by protein saccharification reaction.

Conceptual diagram showing the reaction pathway of protein saccharification reaction The figure which shows the test result about the AGEs decomposition rate of each extract The figure which shows the test result about the AGEs decomposition rate of each extract

Embodiments of the present invention will be described below. In addition, this invention should not be limited to these embodiments at all, and can be implemented in various modes without departing from the gist thereof.
<Example>
<Overview>

This example relates to an AGEs decomposing agent that decomposes fluorescent AGEs. As an AGEs decomposing agent, extracts of plants belonging to the family Lamiaceae, Rosaceae, Oleaceae, Asteraceae, Nymphalidae, Dokudamiaceae, Euphorbiaceae, Gramineae, Azalea, especially plants belonging to these families The extract obtained as a tea outside tea is used.
<Configuration>

  The AGEs in this example are AGEs having fluorescence with an excitation wavelength of about 370 nm and a detection wavelength of about 440 nm. Such fluorescence is a physicochemical characteristic of the final product of the protein saccharification reaction. Note that fluorescent AGEs include pentosidine, croslin, pyropyridine, and the like, but there are many AGEs whose substance structures have not yet been identified, and are not limited thereto.

  The plant extract in this example may be extracted from any part of the plant, for example, extracted from whole grass, flowers, seeds, fruits, branches, stems, bark, roots, etc. Good. Moreover, those parts extracted from tea using the respective parts as materials may be used.

  Here, “tea” in this example is not only a tea leaves or stems that are processed into a beverage by pouring hot water or water into it, but also petals or fruits of other plants are mixed with the leaves of tea trees. In addition to so-called flavored tea and flower tea, which are used as beverages, the leaves, buds, flowers, bark, roots, etc. of plants other than tea tree are processed without using tea leaves, and hot water or water is poured into the beverage. It also contains so-called tea outside tea. What is known as tea outside tea includes, for example, dokudami tea, mochi tea, rooibos tea and the like.

  Below, the plant and tea used in a present Example are demonstrated.

  “Misohagiidae” belongs to the order of Myrtaceae and has the genus Misohagi, Crape myrtle, genus Kakashigusa, etc. in the subclass. In the test to be described later, Banaba tea having approximately 100% of the leaf material of “Lagerstroemia sp.” Was used as a sample of the plant family plant extract. This banaba tea is tea without tea.

  "Rosaceae" belongs to the order of roses, and has the genus Rose, Raspberry, Shimotsuki, Hawthorn, etc. in the subclass. In the test described later, Meigui tea and rosehip tea were used as samples of the Rosaceae plant extract.

  Meigui tea is a tea-free tea that uses approximately 100% of the buds of the flowers of the rose genus “Rosa rugosa”. Rosehip tea is tea made from rosehips. In the case of tea, “Rosa canina” of the genus Rose is often used. Also in this example, rosehip tea was used in which the fruit of the dog rose is approximately 100% of the raw material.

  The "Coleaceae" belongs to the order of Labiatae, and has a subclass such as genus Moxae, genus Sokei and ash genus. In the test to be described later, jasmine tea obtained by mixing a flower of Jasmine sp.

  The "Asteraceae" belongs to the order of Asteraceae, and has a subclass such as a genus of thistle, a genus Artemisia, a genus Chrysanthemum, a genus Sunflower, and a genus sunflower. In the test described below, chicory tea, dandelion root tea, chamomile tea, and burdock tea were used as the Asteraceae plant extract.

  Chicory tea is a tea-free tea that uses approximately 100% of the raw material of the root of “Chicorium intybus” of the genus Kycholium. The dandelion root tea is a tea-outer tea whose root is approximately 100% of the raw material of the genus “Taraxacum officinale”. Chamomile tea is a tea-outer tea that uses approximately 100% of the raw material of “Chamomile (Matricaria recutia)” flowers. Moreover, burdock tea is a tea outside tea that uses approximately 100% of the raw material of the root of the genus “burdock (Arcium lappa)”.

  The “Nymphalidae” belongs to the order of the elegans, and has the genus Nikikigi, the genus Zodiac and the like in a subclass. In the test to be described later, Salacia tea, which uses 100% of the whole plant of the Salacia genus “Salacia oblonga” as a raw material, was used as a sample of the asteraceae plant extract.

  "Dokudamiidae" belongs to the order of Pepper, and has a genus Dokudami, Hangesho, etc. in a subclass. In the test to be described later, as a sample of the scorpionaceae plant extract, dokudami tea with leaves and stems of “Houttunia corda” belonging to the genus Dokudami was used as approximately 100% of the raw material.

  The “Rulaceae” is a plant belonging to the genus Euphorbiaceae (Gallia, depending on the classification). In the test to be described later, eucommia tea containing approximately 100% of the leaves of Eucommia ulmoides as the raw material was used as a sample of the eucommia plant extract.

  “Gramineae” belongs to the order of the rice, and has a subclass such as Sasa, Gramineae, Strawberry genus, Reed genus and the like. In the test to be described later, as a sample of the Gramineae plant extract, brown rice in which the brown rice of glutinous rice and glutinous rice belonging to the rice genus “Oryza sativa” is roasted and mixed is approximately 100% of the raw material. Tea was used. This brown rice tea is a tea outside tea that does not use tea leaves of chanoki, unlike the general “brown rice tea” in which fried brown rice is mixed with tea leaves of chanoki.

  The “Azalea family” belongs to the order of the azalea and has the genus Acebi, Azalea, Erica, Kalmia and the like in a subclass. In the test to be described later, blueberry tea containing substantially 100% of the fruit of “Vaccinium corymbosum” belonging to the genus Vaccinium was used as a sample of an azalea plant extract.

  The AGEs decomposing agent containing one or more selected from plant extracts including each of the above-mentioned samples can be used for foods, foods, health foods, and food additives containing the AGEs decomposing agent by using a known method. It can be provided as cosmetics, pharmaceuticals, quasi drugs, and the like.

  In the case of a pharmaceutical product, the antioxidant mixture of the present embodiment is made into powder or granules and filled into a capsule, or an excipient, a binder, a disintegrant, etc. are added and a tableting machine or the like is used. Can be manufactured. In addition, in the case of health foods and supplements, it may be provided in the form of capsules or tablets like pharmaceuticals, or an antioxidant mixture added to various foods such as beverages, seasonings and confectionery Can also be provided.

Moreover, it can also be set as cosmetics, such as a cosmetic liquid, cream, and lotion. For example, in the case of a beauty essence, in addition to the antioxidant mixture of the present embodiment, water, rice bran oil, pentylene glycol, glycerin, squalane, cetyl palmitate, dimer dilinoleic acid and the like as main components, and sodium hyaluronate Hydrogenated rapeseed oil alcohol, carbomer, xanthan gum, hydroxylated K, dimethicone, polysorbate-60, glyceryl stearate, hydrogenated castor oil, phenoxyethanol, urea, arginine, arbutin, citric acid and the like are used as additives. Then, after each component is dissolved in a water-soluble raw material and an oil-soluble raw material, they are heated and mixed and emulsified. While cooling this, an additive such as an extract is blended, and a highly volatile substance such as essential oil or fragrance is added when the temperature becomes lower. Thereafter, a predetermined safety test (bacteria, pH, temperature stability, viscosity, etc.) is performed, and the product can be provided as a product after being filled into a bottle. Moreover, it is also preferable to further improve the antioxidant effect as a product by further adding an antioxidant component such as astaxanthin, tocopherol, ubiquinone, Na ascorbate. It is also possible to add parabens as a preservative.
<Test>

  Banana tea, Meigui tea, rosehip tea, jasmine tea, chicory tea, dandelion root tea, chamomile tea, burdock tea, dokudami tea, salacia tea, eucommia tea, brown rice tea, blueberry tea About the extract from each tea leaf of tea, the test of the AGEs decomposition | disassembly effect | action was done as follows. In addition, a tea leaf means the banaba leaf processed and manufactured in order to serve as banaba tea by pouring hot water or water, for example in banaba tea. Similarly, the flowers of the genus in the magui tea and the fruit of the dog rose in the rose hip tea are also tea leaves.

  First, 3.75 g of each tea leaf was put into 150 mL of distilled water at 80 ° C. and extracted for 1 hour in a water bath set at 80 ° C. Thereafter, the extracted solution was centrifuged at 1,500 × g for 15 minutes, and the supernatant was used as an extract.

  AGEs HSA (AGE-HSA) was prepared as an evaluation substrate for AGEs degradation action. Specifically, 25 mL of 100 mmol / L phosphate buffer (pH 7.4), 2 mL of 2 mol / L glucose (Glc), 10 mL of 40 mg / mL human serum albumin (HSA), and 10 mL of distilled water were mixed and incubated at 60 ° C. for 40 hours. The obtained reaction solution was designated as AGE-HSA.

To evaluate the AGEs decomposition action of each extract, each reaction solution was mixed according to the following Table 1 and incubated at 37 ° C. for 90 minutes.

  Thereafter, 100 μL of 7% perchloric acid (PCA) was added to the reaction solution, cooled on ice for 20 minutes, and centrifuged at 1,500 × g at 4 ° C. to precipitate proteins. After removing the supernatant, 350 μL of 200 mmol / L Tris-HCl (pH 7.4) was added to the precipitated protein and stirred, and the whole amount was redissolved in an amount (250 μL) before precipitation. 250 μL of the redissolved solution was added to each 96-well microplate (black), and AGEs-derived fluorescence at an excitation wavelength of 370 nm and a detection wavelength of 440 nm was measured with a microplate reader. The fluorescence value of the measured redissolved solution was determined as a relative value when the value obtained by measuring a 5 μL / mL quinine sulfate solution was 1,000, and then the AGE decomposition rate was calculated according to the following formula.

(Formula 1) AGE decomposition rate (%) = (1−A / B) × 100
<Result>

  The AGE decomposition rate of each extract is shown in FIG. As shown in the figure, Banaba tea is 40% or more, Meigui tea, Jasmine tea, Chicory tea is 20% or more and less than 30%, Dandelion root tea, Chamomile tea, Salacia tea, Dokudami tea, Burdock tea, Rosehip tea, Tochu tea. Brown rice tea and blueberry tea were 10% or more and less than 20%.

  In addition, the AGEs degradation action evaluation test was similarly performed by changing the evaluation substrate to AGE-modified collagen (derived from cowhide). Each reaction solution was incubated at 37 ° C. for 18 hours, and then the fluorescence value derived from AGEs was measured as described above, and the AGE decomposition rate was calculated according to the above formula 1.

  FIG. 3 shows the degradation rate for AGEs collagen. As shown in the figure, it is over 100% for chamomile tea and banaba tea, over 50% for Meigui tea and jasmine tea, and over 10% for dandelion tea, Salacia tea, chicory tea, dokudami tea and burdock tea. Met.

As described above, it was possible to confirm the AGEs decomposition action for each of the tea extracts made from 13 types of plants. All of these plants can effectively decompose AGEs by ingesting as tea without resistance. In addition, by combining them, it is possible to adjust various tastes and meet various taste demands.
<Effect>

  ADVANTAGE OF THE INVENTION By this invention, the AGEs decomposition agent which decomposes | disassembles effectively AGEs which are saccharification reaction final products can be provided. Moreover, it can provide as such tea which is easy to ingest such an AGEs decomposition agent.

Claims (9)

  An AGEs degrading agent having an action of degrading AGEs, comprising at least one selected from the extracts of plants belonging to the family Moleaceae, Euphoridae, Dokudamiaceae, Euphorbiaceae and Gramineae.   An AGEs degrading agent containing one or more selected from banaba, mayi, rosehip, jasmine, chicory, dandelion, chamomile, burdock, dokudami, salasia, eucommia, brown rice, and blueberry extract, and has an action of degrading AGEs .   Banaba tea, Meigui tea, Rosehip tea, Jasmine tea, Chicory tea, Dandelion root tea, Chamomile tea, Burdock tea, Dokdami tea, Salacia tea, Tochu tea, Brown rice tea, Blueberry tea extract The AGEs decomposition agent which has the effect | action which contains the above and decomposes | disassembles AGEs.   Food / beverage containing the AGEs decomposition agent as described in any one of Claim 1 to 3.   A health food containing the AGE degrading agent according to any one of claims 1 to 3.   The food additive containing the AGEs decomposition agent as described in any one of Claim 1 to 3.   Cosmetics containing the AGEs decomposition agent as described in any one of Claim 1 to 3.   A pharmaceutical comprising the AGE degrading agent according to any one of claims 1 to 3.   A quasi-drug containing the AGE degrading agent according to any one of claims 1 to 3.

Images