TW201517930A - Use of starfish extract for manufacture of composition for enhancing secretion of collagen - Google Patents
Use of starfish extract for manufacture of composition for enhancing secretion of collagen Download PDFInfo
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本發明係關於一種促進膠原蛋白增生之用途,特別是關於一種海星萃取物在製備促進膠原蛋白增生的組合物之用途。 The present invention relates to a use for promoting collagen hyperplasia, and more particularly to the use of a starfish extract for the preparation of a composition for promoting collagen proliferation.
海星為棘皮動物門下的一個綱的動物的名稱,具有驚人的再生能力,不僅能再生出斷掉的腕足,某些海星甚至可由被截斷的腕足本身再生成一隻完整的新個體。海星多分佈於亞洲、北美洲、澳洲的沿海地區,棲息地通常為沙泥底質的海底。臺灣屬於海島,水產豐富,亦有多種的海星,例如在澎湖的潮間帶常見的飛白楓海星(Archaster typicus)。海星的用途廣泛,某些海星除了可提供作為食材或加工食品的材料外,亦是一種常被使用的中藥材;海星因為其高的營養價值,亦被加工成粉狀飼料。在醫學用途方面,海星經提煉後可用來製作對人體無害的替代血漿;此外,有研究指出,海星所含的二十五碳五烯酸等成分,對高血脂、心血管疾病的治療,有一定的效果。 Starfish is the name of an animal under the echinoderms. It has an amazing ability to regenerate. It not only regenerates the broken brachiopod, but some starfish can even regenerate a complete new individual from the truncated wrist. Starfish are mostly distributed in coastal areas of Asia, North America, and Australia, and the habitat is usually sandy bottom. Taiwan is an island with abundant aquatic products. There are also many kinds of starfish, such as Archaster typicus , which is common in the intertidal zone of Penghu. Starfish are widely used. In addition to being used as a food or processed food, some starfish are also a commonly used Chinese herbal medicine. Starfish are also processed into powdered feed because of its high nutritional value. In terms of medical use, the starfish can be used to make substitute plasma that is harmless to the human body after refining; in addition, studies have indicated that the components of the starfish, such as eicosapentaenoic acid, are useful in the treatment of hyperlipidemia and cardiovascular diseases. Certain effect.
膠原蛋白是一種生物性高分子物質,是脊椎動物體內含量最多的蛋白質,在人體內則占總蛋白質的三分之一。主要存在於結締組織的細胞外間質(extracellular matrix)中,是細胞與細胞間的支架。人體的皮膚、 軟骨、角膜、血管壁、內臟器官等均含有膠原蛋白,尤其是皮膚,膠原蛋白提供支撐皮膚細胞的強度,保持皮膚的緊緻與彈性。然而,隨著年齡增長及所受外在不利因素的累積,皮膚纖維母細胞生成膠原蛋白的能力會降低,一旦膠原蛋白流失的速度大於其生成的速度,皮膚便會產生皺紋、鬆弛等現象。 Collagen is a biological polymer substance that is the most abundant protein in vertebrates and accounts for one-third of total protein in humans. It is mainly found in the extracellular matrix of connective tissue and is a scaffold between cells and cells. Human skin, Cartilage, cornea, blood vessel wall, internal organs and so on all contain collagen, especially the skin. Collagen provides the strength to support skin cells, keeping the skin firm and elastic. However, with the increase of age and the accumulation of external unfavorable factors, the ability of skin fibroblasts to produce collagen will decrease. Once the rate of collagen loss is greater than the rate of its formation, the skin will produce wrinkles and sagging.
因此,補充膠原蛋白或是提升細胞的膠原蛋白生成能力是皮膚保養的重要課題。市面上不乏補充膠原蛋白的產品,包括以外用塗抹、注射或食用等方式。然而,膠原蛋白的分子大,不易穿透皮膚的角質層,塗抹方式幾乎無法使膠原蛋白到達其所作用之真皮層皮膚纖維母細胞;若是以注射的方式直接補充膠原蛋白,雖然可立即減少皮膚的細紋,但該注射進入體內的膠原蛋白會被體內的酵素分解,需定期的施打,過程繁複且成本高,且注射膠原蛋白可能產生免疫排斥反應,增加使用上的風險。若是以食用富含膠原蛋白的食品來補充膠原蛋白,膠原蛋白進到腸胃後,其三股螺旋的結構即被破壞形成多胜肽,多胜肽再經由消化液切斷成小分子、最後分解成胺基酸。身體固然會利用這些胺基酸合成蛋白質,但不限於膠原蛋白,因此實際補充膠原蛋白的效果有限。 Therefore, supplementing collagen or enhancing the collagen production ability of cells is an important issue in skin care. There are many products that supplement collagen in the market, including external application, injection or consumption. However, the molecular size of collagen is too large to penetrate the stratum corneum of the skin. The application method can hardly make the collagen reach the dermal layer of the fibroblasts. If the collagen is directly added by injection, the skin can be immediately reduced. The fine lines, but the collagen injected into the body will be decomposed by the enzymes in the body, requiring regular application, the process is complicated and costly, and the injection of collagen may cause immune rejection and increase the risk of use. If collagen is added to eat collagen-rich foods, and the collagen enters the stomach, the structure of the triple helix is destroyed to form a multi-peptide, which is then cleaved into small molecules through the digestive juice and finally broken down into Amino acid. Although the body uses these amino acids to synthesize proteins, but not limited to collagen, the actual effect of collagen supplementation is limited.
有鑑於此,提升人體皮膚纖維母細胞的膠原蛋白生成能力係較補充膠原蛋白更能確保有效的產生及利用膠原蛋白,然而目前市面上尚無一種安全、有效的產品,可促進膠原蛋白的生成。 In view of this, the ability to increase collagen production in human skin fibroblasts is more effective than collagen supplementation to ensure efficient production and utilization of collagen. However, there is currently no safe and effective product on the market that promotes collagen production. .
緣此,本發明之一目的係提供一種海星萃取物在製備促進膠原蛋白增生的組合物之用途,其中該海星萃取物包含水溶性多醣體,且該 水溶性多醣體係為海星多醣。該海星萃取物能提昇一細胞的膠原蛋白增生率,該細胞係為一皮膚纖維母細胞且來自於人類;本發明之海星萃取物能促進該皮膚纖維母細胞的膠原蛋白增生率為80%以上。 Accordingly, it is an object of the present invention to provide a use of a starfish extract for preparing a composition for promoting collagen proliferation, wherein the starfish extract comprises a water-soluble polysaccharide, and The water soluble polysaccharide system is a starfish polysaccharide. The starfish extract can increase the collagen proliferation rate of a cell which is a skin fibroblast and is derived from a human; the starfish extract of the invention can promote the collagen fibroblast proliferation rate of the skin fibroblast of 80% or more. .
在本發明之一實施例中,該海星萃取物係由海星活體萃取而得,係海星活體經由冷凍、磨粉、高溫萃取及乙醇萃取所製成。 In one embodiment of the present invention, the starfish extract is obtained by extracting a starfish in vivo, and the starfish living body is prepared by freezing, milling, high temperature extraction, and ethanol extraction.
此外,本發明之海星萃取物所製備之組合物可為一外用擦劑、或一外用醫藥品、或一外用保養品、或一化妝品。需說明的是,所稱之外用擦劑或外用醫藥品包括但不限於軟膏、或乳霜劑、或貼附劑或貼附藥布、或含有活性成分之人工組織。另外,所稱之外用保養品或化妝品可包括但不限於化妝水、或化妝乳霜、或乳液、或粉底、或粉底液、或防曬乳、或沐浴劑。 Further, the composition prepared by the starfish extract of the present invention may be an external rubbing agent, or a topical pharmaceutical product, or an external skin care product, or a cosmetic. It should be noted that the external rubbing or external pharmaceutical products include, but are not limited to, an ointment, or a cream, or a patch or a patch, or an artificial tissue containing an active ingredient. Further, the external skin care product or cosmetic may include, but is not limited to, a lotion, or a cosmetic cream, or an emulsion, or a foundation, or a foundation, or a sunscreen lotion, or a body wash.
本發明之另一目的係提供一種提昇細胞增生膠原蛋白之促進劑,其包含一海星萃取物,其中該細胞係為一皮膚纖維母細胞,且該海星萃取物包含89%(w/w)以上之水溶性多醣體。 Another object of the present invention is to provide an agent for promoting cell proliferation collagen, comprising a starfish extract, wherein the cell line is a skin fibroblast, and the starfish extract contains 89% (w/w) or more. a water soluble polysaccharide.
本發明之海星萃取物含有活性成分,並且可顯著地促進皮膚纖維母細胞生成膠原蛋白。本發明之海星萃取物,其所包含之海星萃取物來源天然,且製備方法簡單、無污染,因此可有效應用於保養肌膚之產品及化粧品。 The starfish extract of the present invention contains an active ingredient and can remarkably promote collagen production by skin fibroblasts. The starfish extract of the invention has the natural source of the starfish extract, and the preparation method is simple and pollution-free, so it can be effectively applied to the skin care products and cosmetics.
以下將配合圖式進一步說明本發明的實施方式,下述所列舉的實施例係用以闡明本發明之發明特點及應用,而非以限定本發明之範圍,任何熟習此技藝者,在不脫離本發明之精神和範圍內,當可做些許更動與潤飾,因此本發明之保護範圍當視後附之申請專利範圍所界定者為準。 The embodiments of the present invention will be further described in conjunction with the drawings, which are set forth to illustrate the features and applications of the present invention, and are not intended to limit the scope of the present invention. In the spirit and scope of the present invention, the scope of the present invention is defined by the scope of the appended claims.
第一圖係本發明海星萃取物之萃取流程圖。 The first figure is a flow chart for extracting the starfish extract of the present invention.
第二圖係本發明海星萃取物促進膠原蛋白增生能力。 The second figure is the ability of the starfish extract of the present invention to promote collagen proliferation.
第三圖係本發明海星萃取物促進皮膚纖維母細胞生成膠原蛋白之螢光染色顯微鏡圖,其中(a)控制組;(b)以5%(v/v)之海星萃取物處理;(c)以表皮生長因子(EGF,5ng/mL)處理。 The third figure is a fluorescent staining micrograph of the starfish extract of the present invention for promoting collagen production of skin fibroblasts, wherein (a) the control group; (b) treated with 5% (v/v) starfish extract; ) treated with epidermal growth factor (EGF, 5 ng/mL).
為證實本發明之海星萃取物能促進膠原蛋白增生,本發明係由溫和、操作簡易之方法製備海星萃取物,並分析海星萃取物中的活性成分。另外,亦將本發明之海星萃取物與皮膚纖維母細胞共同培養,評估本發明之海星萃取物提升皮膚纖維母細胞之膠原蛋白優異生成能力。 In order to confirm that the starfish extract of the present invention can promote collagen proliferation, the present invention prepares a starfish extract by a gentle and easy-to-use method, and analyzes the active ingredient in the starfish extract. Further, the starfish extract of the present invention was also co-cultured with skin fibroblasts, and the starfish extract of the present invention was evaluated to enhance the collagen-producing ability of the skin fibroblasts.
本發明之海星萃取物為可提升皮膚纖維母細胞之膠原蛋白生成率,足以實現此目的之含量係定義為「有效劑量」。可實現此目的之含量將根據熟悉此項技藝者已知之諸多因素來決定,諸如消費者的體重、全身狀態、年齡、性別及組成物基質之效果。 The starfish extract of the present invention is a collagen production rate which can increase the skin fibroblasts, and the content which is sufficient for the purpose is defined as an "effective dose". The amount at which this can be achieved will be determined according to a number of factors known to those skilled in the art, such as the consumer's weight, general condition, age, sex, and the effect of the composition matrix.
本發明所使用之飛白楓海星(Archaster typicus)係收集自臺灣澎湖地區。關於海星萃取物的製備,請參考第一圖。於製備本發明之海星萃取物時,首先,收集海星活體並於捕獲時立即置於-4℃~-20℃環境冷凍儲存,將冷凍之海星活體研磨(如步驟S1);接著,將經研磨之海星以1:3~1:20 (w:v)比例溶於水並加熱至90℃~100℃萃取1~5小時(如步驟S2);高溫萃取後,以離心作用(4,300g)過濾並取其上清液(如步驟S3);接著,於4℃加入3倍體積之乙醇使多醣體沉澱並維持16-24小時(如步驟S4);乙醇沉澱作用後,以離心作用(4,300g rpm)過濾並取其沉澱物(如步驟S5);接著,將該沉澱物溶於10-15倍體積之水中(如步驟S6);最後,以離心作用(4,300g rpm)過濾並取其上清液(如步驟S7),即得到本發明之海星萃取物。 The archery typicus used in the present invention is collected from the Wuhu region of Taiwan. For the preparation of starfish extract, please refer to the first figure. In the preparation of the starfish extract of the present invention, first, the living starfish is collected and immediately stored at -4 ° C ~ -20 ° C during storage, and the frozen starfish is ground in vivo (as in step S1); The starfish is dissolved in water at a ratio of 1:3 to 1:20 (w:v) and heated to 90 ° C to 100 ° C for 1 to 5 hours (as in step S2); after high temperature extraction, it is filtered by centrifugation (4,300 g). And taking the supernatant (such as step S3); then, adding 3 volumes of ethanol at 4 ° C to precipitate the polysaccharide for 16-24 hours (as in step S4); after ethanol precipitation, by centrifugation (4,300g) Rpm) filter and take the precipitate (as in step S5); then, the precipitate is dissolved in 10-15 volumes of water (as in step S6); finally, it is filtered by centrifugation (4,300 g rpm) and taken up The clear liquid (as in step S7), the starfish extract of the present invention is obtained.
本發明之海星萃取物的總多醣體含量係以酚-硫酸(phenol-sulfuric acid)法測定。將100μL海星萃取物與不同濃度的葡萄糖標準溶液(0、25、50、75、100及125mg/mL)混合於試管中。接著加入0.5mL酚溶液與2.5mL硫酸進行反應。將試管以光譜訊號感測器(spectrophotometer)測量其在750nm之吸光值。 The total polysaccharide content of the starfish extract of the present invention is determined by the phenol-sulfuric acid method. 100 μL of starfish extract was mixed with different concentrations of glucose standard solutions (0, 25, 50, 75, 100, and 125 mg/mL) in test tubes. Next, 0.5 mL of a phenol solution was added and reacted with 2.5 mL of sulfuric acid. The tube was measured for its absorbance at 750 nm as a spectrophotometer.
分析結果顯示,在2.5%(w/v)之海星萃取物溶液中含有2.23%(w/v)之水溶性多醣體,因此可知本發明之海星萃取物所含有的水溶性多醣體含量大約為89%(w/w)。 The analysis results show that 2.2% (w/v) of the water-soluble polysaccharide is contained in the 2.5% (w/v) starfish extract solution, so that the seawater extract of the present invention contains a water-soluble polysaccharide content of about 89% (w/w).
以含有10%胎牛血清(FBS)之最低必需培養基(MEM)培養人類皮膚纖維母細胞CCD-966SK(Human skin fibroblast cells,購自食品工業發展研究所)於24-孔盤,人類皮膚纖維母細胞之濃度為4×104細胞/孔。經過24小時培養後更新培養基。實驗組為以磷酸鹽緩衝溶液(phosphate buffer saline,PBS)稀釋海星萃取物(濃度分別為0.2、0.4、0.6、0.8、及1.0mg/mL)並加入各孔中。控制組則未加入海星萃取物,並於37℃、5%二氧化碳條件 下培養48小時後取其上清液,實驗發現海星萃取物促進膠原蛋白的生成在48小時以內隨時間增加而有線性相關。 Human skin fibroblasts CCD-966SK (Human skin fibroblast cells, purchased from Food Industry Development Institute) in 24-well plates, human skin fibrils, in minimal essential medium (MEM) containing 10% fetal bovine serum (FBS) The concentration of the cells was 4 x 10 4 cells/well. The medium was updated after 24 hours of culture. In the experimental group, the starfish extracts (concentrations of 0.2, 0.4, 0.6, 0.8, and 1.0 mg/mL, respectively) were diluted with phosphate buffer saline (PBS) and added to each well. In the control group, the starfish extract was not added, and the supernatant was cultured at 37 ° C under 5% carbon dioxide for 48 hours. It was found that the starfish extract promoted collagen production and increased linearly with time within 48 hours. .
使用Sircol® Collagen Assay Kit(Bicolor Life Science Assays,Northern Ireland,UK)分析水溶性第I型膠原蛋白的含量。在室溫下,將上述方法所得之上清液100μL與1mL Sircol®染劑混合30分鐘。接著以15,000g離心作用10分鐘以形成膠原蛋白沉澱物。去除離心後的上清液並將膠原蛋白沉澱物溶解於1mL鹼性溶劑。以光譜訊號感測器測量其在波長540nm的吸光值。膠原蛋白的數量係以水溶性膠原蛋白標準曲線(標準無疾病牛皮膚第I型膠原蛋白,美國)計算。 The content of the water-soluble type I collagen was analyzed using a Sircol® Collagen Assay Kit (Bicolor Life Science Assays, Northern Ireland, UK). At room temperature, the supernatant obtained in the above method of 100μL with 1mL Sircol ® dyes mixed for 30 minutes. This was followed by centrifugation at 15,000 g for 10 minutes to form a collagen precipitate. The supernatant after centrifugation was removed and the collagen precipitate was dissolved in 1 mL of an alkaline solvent. The absorbance at a wavelength of 540 nm was measured with a spectral signal sensor. The amount of collagen was calculated as a water-soluble collagen standard curve (standard disease-free bovine skin type I collagen, USA).
請參考第二圖,該圖係本發明之海星萃取物促進膠原蛋白生成之分析,發現實驗組以1mg/mL之海星萃取物處理後,每單位人類皮膚纖維母細胞生成45.25ρg的膠原蛋白;遠高於於控制組生成24.48ρg膠原蛋白的量,因此,本發明之海星萃取物明顯證實可有效促進膠原蛋白增生率至少80%以上,較佳達84.8%。 Please refer to the second figure, which is an analysis of the collagen production of the present invention to promote collagen production, and found that the experimental group produced 45.25 ρg of collagen per unit of human skin fibroblasts after treatment with 1 mg/mL of starfish extract; It is much higher than the amount of 24.48 ρg of collagen produced by the control group. Therefore, the starfish extract of the present invention clearly demonstrates that it can effectively promote the collagen proliferation rate of at least 80% or more, preferably 84.8%.
另外以含有1%多聚甲醛(para-formaldehyde,Sigma,法國)之磷酸鹽緩衝溶液(PBS)固定人類皮膚纖維母細胞CCD-966SK30分鐘後,並於磷酸鹽緩衝溶液中沖洗。以本發明之海星萃取物(5%(v/v))、表皮生長因子(5ng/mL)分別處理該細胞24小時,控制組細胞則以生理食鹽水(Phosphate Buffered Saline,PBS)處理。再將該經海星萃取物或表皮生長因子各別處理之細胞與抗水溶性第I型膠原蛋白大鼠單株抗體(1/2000稀釋,abcam,英國)共同培養於含有1%(w/v)牛血清蛋白(PBS)之磷酸鹽緩衝溶液。接著,以磷 酸鹽緩衝溶液徹底地清洗並與以螢光素(fluorescein)標記之大鼠IgG抗體(1/200稀釋,KPL,美國)在無光環境下共同培養60分鐘。以磷酸鹽緩衝溶液清洗後,以螢光共軛焦顯微鏡(Leica TSC SP2,德國)觀察。 The human skin fibroblast CCD-966SK was fixed in phosphate buffered saline (PBS) containing 1% paraformaldehyde (Sigma, France) for 30 minutes and washed in a phosphate buffer solution. The cells were treated with the starfish extract (5% (v/v)), epidermal growth factor (5 ng/mL) of the present invention for 24 hours, and the control cells were treated with physiological saline (Phosphate Buffered Saline, PBS). The cells treated with the starfish extract or the epidermal growth factor were co-cultured with the water-soluble type I collagen rat monoclonal antibody (1/2000 dilution, abcam, UK) at 1% (w/v). a phosphate buffer solution of bovine serum albumin (PBS). Next, with phosphorus The acid salt buffer solution was thoroughly washed and co-cultured with fluorescein-labeled rat IgG antibody (1/200 dilution, KPL, USA) in a light-free environment for 60 minutes. After washing with a phosphate buffer solution, it was observed with a fluorescent conjugated focus microscope (Leica TSC SP2, Germany).
請參考第三圖,該圖係皮膚纖維母細胞生成膠原蛋白之螢光染色顯微鏡圖,其中(a)代表控制組細胞,(b)代表以5%(v/v)海星萃取物處理之細胞,(c)代表以表皮生長因子(5ng/mL)處理之細胞。圖中箭頭表示膠原蛋白。由第三圖清楚可見,經過本發明之海星萃取物處理之細胞,較控制組細胞大幅增加膠原蛋白之生成量,亦較以表皮生長因子處理之細胞生成更多的膠原蛋白,因此,本發明之海星萃取物進一步證實可有效並且顯著地促進膠原蛋白的增生。 Please refer to the third figure, which is a fluorescence staining micrograph of collagen produced by skin fibroblasts, where (a) represents the control group cells, and (b) represents cells treated with 5% (v/v) starfish extract. (c) represents cells treated with epidermal growth factor (5 ng/mL). The arrows in the figure indicate collagen. As is clear from the third figure, the cells treated by the starfish extract of the present invention substantially increase the amount of collagen produced by the control group, and produce more collagen than the cells treated with the epidermal growth factor. Therefore, the present invention The starfish extract was further confirmed to be effective and significantly promote collagen proliferation.
同時,本發明之海星萃取物亦包含多種胺基酸及微量元素,分析結果請參考下表。 At the same time, the starfish extract of the present invention also contains various amino acids and trace elements. For the analysis results, please refer to the following table.
以海星萃取物可產製油包水(O/W)中性乳霜及凝膠等化妝
品及/或醫藥品。O/W中性乳霜油相約30~50%左右,不致有過於油膩之感受,可添加海星萃取物於配方中,具有促進皮膚膠原蛋白增生之效果,將本發明所得之海星萃取物應用於中性乳霜之配方如表三所示:
在高速狀態下緩慢加入油相之成分,待乳化完成後,即可得含海星萃取物之乳霜,該乳霜可在室溫下保存6個月。 The oil phase component is slowly added at a high speed, and after the emulsification is completed, a cream containing a starfish extract can be obtained, which can be stored at room temperature for 6 months.
本發明所得之海星萃取物亦可用於產製膠原蛋白凝膠,該凝膠亦可達到促進皮膚膠原蛋白增生之效果,該凝膠之配方如表四所示:
本發明之海星萃取物可用於產製促進膠原蛋白增生之精華液,包含一海星萃取物組份、一保濕功能成分組份、一美白功能成分組份及一抑菌劑。促進膠原蛋白增生之精華液係以海洋深層水作為基質,其含量實質上是大約40wt%,其中,該促進膠原蛋白增生之精華液的總計重,該海星萃取物組份的含量是介於15wt%至20wt%;該保濕功能成分組份包括但不限於選自於由醣蛋白(Glygoprotein)、玻尿酸、1,3-丁二醇、或1,3-丙二醇所組成之群組,其含量是介於10wt%至20wt%;該美白功能成分組份包括但不限於選自於由熊果葉素(Arbutin)、麴酸(Kojic Acid)、維生素C磷酸鈉(Sodium Ascorbyl Phosphate)、或維生素C磷酸鎂(Magnesium Ascorbyl Phosphate)所組成之群組,其含量是介於10wt%至20wt%;而該抑菌劑的含量實質上是0.1wt%至0.2wt%。 The starfish extract of the invention can be used for producing an essence for promoting collagen proliferation, comprising a starfish extract component, a moisturizing functional component, a whitening functional component and a bacteriostatic agent. The essence for promoting collagen hyperplasia is a deep ocean water as a matrix, and the content thereof is substantially about 40% by weight, wherein the total weight of the collagen-promoting essence is increased, and the content of the starfish extract component is 15 wt%. % to 20% by weight; the moisturizing functional ingredient comprises, but is not limited to, selected from the group consisting of Glygoprotein, Hyaluronic Acid, 1,3-Butanediol, or 1,3-propanediol, the content of which is Between 10% and 20% by weight; the whitening functional component includes, but is not limited to, selected from Arbutin, Kojic Acid, Sodium Ascorbyl Phosphate, or Vitamin C. The group consisting of magnesium nitrate (Magnesium Ascorbyl Phosphate) is contained in an amount of from 10% by weight to 20% by weight; and the bacteriostatic agent is contained in an amount of from 0.1% by weight to 0.2% by weight.
本發明之海星萃取物用於產製精華液之效果在於:精華液中海星萃取物組份含有水溶性多醣,即海星多醣,可促進皮膚纖維母細胞之 膠原蛋白增生率,且海星萃取物中亦含有多種胺基酸及微量元素,可活化皮膚細胞,搭配精華液中之保濕功能組份及美白功能組份,可進一步提升精華液對皮膚的保養功效。 The effect of the starfish extract of the present invention for producing an essence is that the starfish extract component in the essence contains a water-soluble polysaccharide, that is, a starfish polysaccharide, which can promote skin fibroblasts. The rate of collagen proliferation, and the starfish extract also contains a variety of amino acids and trace elements, which can activate skin cells, and with the moisturizing functional components and whitening functional components in the essence, can further enhance the skin's maintenance effect on the skin. .
綜上所述,本發明之海星萃取物具有多種活性成分,且富含水溶性多醣體,可促進皮膚纖維母細胞之膠原蛋白增生,此外,本發明之海星萃取物的製造方法簡易、無污染,因此不僅可有效利用天然資源而不對環境造成傷害,亦可提供一高活性且安全之肌膚保養品原料。 In summary, the starfish extract of the present invention has various active ingredients and is rich in water-soluble polysaccharides, which can promote collagen proliferation of skin fibroblasts. In addition, the method for producing starfish extract of the present invention is simple and non-polluting. Therefore, it can not only effectively use natural resources without harming the environment, but also provide a highly active and safe skin care product.
惟,以上之敘述僅為本發明之較佳實施例說明,凡精於此項技藝者當可依據上述之說明而作其它種種之改良,惟這些改變仍屬於本發明之精神及以下所界定之專利範圍中。 However, the above description is only for the preferred embodiment of the present invention, and those skilled in the art can make various other modifications according to the above description, but the changes are still within the spirit of the present invention and as defined below. In the scope of patents.
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