TW201040280A - Carbohydrate binding module and use thereof - Google Patents
Carbohydrate binding module and use thereof Download PDFInfo
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- TW201040280A TW201040280A TW098124661A TW98124661A TW201040280A TW 201040280 A TW201040280 A TW 201040280A TW 098124661 A TW098124661 A TW 098124661A TW 98124661 A TW98124661 A TW 98124661A TW 201040280 A TW201040280 A TW 201040280A
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- 108010003885 valyl-prolyl-glycyl-glycine Proteins 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
- C07K16/1036—Retroviridae, e.g. leukemia viruses
- C07K16/1045—Lentiviridae, e.g. HIV, FIV, SIV
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/18—Antivirals for RNA viruses for HIV
Description
201040280 六、發明說明： 【發明所屬之技術領域】 本發明係關於醣類結合模組及其應用。特別是醣類結合模組可結合 mv之醣蛋白，為新穎的抗體模擬物。 【先前技術】 已知人類免疫缺乏病毒(HIV)係引起後天免疫缺乏症候群(AI〇s)的 主因。由於HIV具快速遺傳變異性致持續產生突變株病毒，因此偵 〇 測及治療不同品系之HIV感染相當困難且深具挑戰性。 一般最常偵測待測樣品中之抗Ηΐν之抗體以診斷ffiv感染。目前 已開發多項HTV感染的實驗室診斷方法提供大量篩檢，以延長hjv 病人之壽命並提昇其生活品質，其中已開發國家最常使用酵素免疫 分析法(EIA)。此法包含一層塗鍍的病毒抗原，其可能由病毒溶解 物、反轉錄濾過性病毒蛋白質、和天然或合成之多肽類組成，此病 毒抗原會和可能含有mv抗體之血液或血清反應。雖然酵素免疫分 析法具有空窗期的問題(產生抗hiv抗體所需之時間限制），由於其 絕佳的靈敏度、專一性及相對較低的花費，此法仍是HIV診斷中最 〇 受歡迎的方法。 然而，在酵素免疫分析法中，使用分離的病毒蛋白作為抗原仍有一 些缺點，例如：培養及處理大量感染性活病毒；活病毒併入測試原 料之可能性；產生的病毒溶解物之多變性質；以及大量可能的偽陰 性及傷陽性結果，需要額外的確認試驗。使用合成的多肽類可運用 免&學及生物資訊的原理設計模擬H】V之抗原決定位(epit〇pe)，避 免上述提到之缺點。然而由於抗原決定位之序列可能隨病毒突變而 降低抗原性甚或不表現，將導致感染血清的偵測失敗。 因此债測HIV感染仍亟需開發可靠、兼具靈敏度及專一性的方法， 且其花費較經濟並可大範圍實施。 3 201040280 ，展-個有效_防性疫苗和其他治雜策略简制膽 臨ίf求。目献部錢韻颇錢㈣錢去活狀病 伴1性及:而=之後天免疫不全病毒的減毒作用仍無法只具 ϊ=ί 因㈣起衫安全軸使得人體臨床 r糾，ffiv已演化_複雜__地逃避套 =蛋白雛之鍾反應，包括糊多_外層防如遮蔽其保留 且穩疋的結構’以及儀立體結構的限制以掩飾其受胖人位置。 因此，近期研究趨勢為以套膜醣蛋白為基_免疫原誘^體。芦 而使用得自雜白12〇之高抗雜％套_單_蛋白12〇或胜 肽’卻只能產生專-性的抗體’ ffij非可廣泛·的抗體。 因此發展-财效_赚和驗性疫苗崎抗卿並抑制現今 流行病，仍舊是一個未實現的目標。 【發明内容】 本發明提供一醣類結合模組之抗體模擬物，其可特異性結合fflv醣 蛋白的一個抗原決定位。 本發明中&及之「酷類結合模組」係指具有酷結合活性之糖類活性 酵素中，一個連續的胺基酸序列。在醣類水解酵素的初級結構分析 中，醣類結合模組分為53個家族，其中包括7個類別例如纖維素、 樹膠質、甲殼素以及澱粉結合。 本發明中提及之「抗體模擬物」係指具有類似抗體結合標的結構之 功能的物體’但其結構較抗體簡單。製造大量抗體需要以下步驟： (a)將單一抗體生產細胞與培養中之腫瘤細胞融合，生成一個融合瘤 細胞’（b)每個融合瘤細胞生產大量之相同的抗體分子，⑹在細胞培 養中增殖融合瘤細胞以產生一個細胞群，其中每個融合瘤細胞都可 生產相同的抗體分子。製造抗體需耗費可觀的人力與費用。然而在 201040280 ίΐΓΙ、’大軸的宿主包括細菌、酵母菌、ι蟲及哺乳動物細胞皆 ° &_結合模纟之抗顧纖，雜紐j_麟經濟。 本發n賴合概之祕實_係贿結合區域 。本發明中提 。之軸結合區域」谢旨—可結合顆減粉或可舰粉之功能性 5域’其可增加酵素活性部位之受質局部濃度’並且可能瓦解殿粉 ，構以增進澱粉分解率。目前共有9健合殿粉之麵結合模 組家族：醣類結合模組家族第20、2卜25、26、34、4卜45、48 Ο ^53族。本發明之触實施财，麟結合區域絲類結合模組 豕族第20和21族’分別選自黑麴菌(々^抑⑽吨以)之葡糖殿粉 酶〇4«SBD)和米根黴(及;^⑽之葡糖殿粉酶(及〇SBD)。雖然 SI類。結合模組家族第2〇和族之胺基酸序列相似度頗低(約 13 ·5/。）’ 一者卻具有非常相似之二級與三級結構，並在顆粒澱粉水 解作用中扮演促進酵素活性之相似角色(Tung JY et al., Biochem. (2008) 416: 27-36)。 本發明之抗體模擬物，其中米根黴葡糖澱粉酶之畴晶片分析顯示其 具有能力結合一多醣Mana(l，2) Mana(l，2)Mana(l，3)Mana，此特殊 O 錄存在於聊1糖蛋白uo之高度醣化的抗原決定位。 人類單株抗體2G12在1996年首先分離出並進行特性分析，已證實 其可中和 HIV1 分支 A 及 B (Trkola A，et al., J. Virol. (1996) 70: 1100-1108)。不同於大多數抗體辨識病毒組成之蛋白質骨架，丙胺 酸定點突變掃描顯示2G12抗原決定位主要位於醣蛋白120上之高 甘露酷類或複雜甘露醣殘基之天門冬胺酸295、天門冬胺酸332、 天門冬胺酸339、天門冬胺酸386、天門冬胺酸392、天門冬胺酸 448(Sanders RW, et al., J. Vir〇l. (2002) 76: 7293-7305 and Scanlan CN, et al” J. Virol. (2002) 76: 7306-7321)。2G12 的晶體結構(PBD ID: 1乙1^)以及其與募糖之複合物]^3119(31(沉心2(1>]81)1〇:1〇1>5)顯示兩 201040280
個抗原結合片段形成一個重鏈變異區交換二聚物(chalarese DA et al.，Science (2003) 300: 2065-2071)。本發明中，米根黴葡糖澱粉酶 及黑麴菌葡糖澱粉酶也能夠辨識HIV之多醣部份。如圖2A及2B 所示，2G12人類單株抗體重鏈變異區之晶體結構(pBD仍：1ZLS) 模型及米根黴葡糖澱粉酶結晶與醣基配體(j>Bd Π): 2V8M)之二級 結構均由貝匕褶板組成’一者之結構及其類似且根均方差 甚低。更進一步根據CATH(蛋白質結構分類資料庫 _"—hdb.inf〇/))顯示，2G12 人類單株抗體(pBD 與黑麴菌葡糖澱粉酶之顆粒蛋白結合區域屬於相同之同源超家族 (ID# 2.6G.4G.1G)，_免疫球蛋白之蛋白f結構組成^米根徵葡糖 殿粉酶、黑麴菌葡糖殿粉酶及2G12之結構相似性，或許顯示其中 之功能相似性。 本發明也提供-侧HIV_r自之雜，糾含：（a) _ 一固體 表面’其上結合能與HIV _蛋白結合的定量之醣類結合模組；⑼ 將樣本與上述表面接觸’以培養形成醣類結合模組_扭¥醣蛋白複合 體；以及(c)培養此複合體，加入標誌mv抗原以產生可量化測量二 訊號’以判讀樣本為HTV感染陽性或陰性。在一較佳實施例中，酿 類結合模組包含但不限於殿粉結合區域。本發明之方法，其中步驟 c所述之抗原，標記-鱗素，該酵素在翻—輯素基質時能夠 產生上述可量化繼之城。其巾之酵素包含但祕於辣根過氧化 氮酶、驗性雜酶、葡糖殿粉酶、尿素酶以及氣黴素乙醯轉換酶。 在一較佳實劇巾，鱗«辣根蛾械酶。對於所屬技術領域 中具有通常知識者而s ’適當之實施例以及使用必要基質進行债測 之手段是眾所皆知的，另外，在-較佳實施财，該基f包括但 限於3,3，，5,5，-四甲基聯苯胺(TMB)。 ~ 所屬技術領域中具有通常知識者能夠理解上述大綱，及—個酵 疫分析法之步驟的描述。他們也能夠理解—般大綱中省略的特定步 201040280 ί之使用適#讀衝崎鱗，㈣料免疫分析 2 將贿鞠_他觸，錢職殊之 $釋說明本發明所屬技術領域中具有通常知識者簡理解， 沾都只是纖性的财，其他相等物在此大财冑有可能。 在本發明中，抗體模擬物之主要標的_ mv之醣蛋白外媒、及穿 膜糖蛋白120。這些酷蛋白質為高度醣化的前驅蛋白質醣蛋白16〇 合^後經高基氏體傳輸時產生醣基化而得。當外套膜醣蛋白12〇結 〇 細胞的主要受11 ’即嵌人可能的標的細胞之細胞膜表面抗原 为無4受體(CD4) ’開啟一個新的感染週期。在本發明中，醣類 結合模組之抗麵擬物不僅可以結合聊套膜醣蛋白之多膽結 構嚙為可偵測和阻斷mv和標的細胞交互作用之可能的治療劑二 此抗體模鳩也可作為-侧防性疫苗，輯抗fflv細糖蛋白之 保留且穩定的抗原決定位，並抑制新的HIV感染。 於上所述’醣類結合模組可作用為抗體模擬物，可應用於傾測_， 且可應用於HIV的預防或治療。 下述的實施例僅作為本發明代表性的不同面向及特徵，而非 制本發明。 【實施方式】 實施例一利用直鏈澱粉親和層析純化米根擻葡糖澱粉酶及黑麴菌 葡糖澱粉酶 ' 分別利用大腸桿菌系統表現載體pET23a及pETl 5Bb製造米根黴葡 糖殿粉酶及黑麴菌葡糖澱粉酶。以結合緩衝液(50 mM醋酸納，酸 驗值5.5)回溶含有重組蛋白質之大腸桿菌菌體，並進行均質處理 201040280 (EmulsiFlex-C5，均質機）。在4。(：、重力_〇〇尽之重力加速度離 心20分鐘以除去細胞碎片，利用直鏈澱粉樹脂(新英格蘭生物實驗 室公司，Ipswich，MA)以親和層析純化上清液。利用5倍管柱體積 之結合緩衝液清洗樹脂後，以丨毫升/分鐘之流速將上清液注入管 柱。再以5倍管柱體積之結合緩衝液清洗樹脂，之後利用洗提緩衝 液(10mM甘氨酸/氫氧化鈉，酸驗值11〇)將重組蛋白質洗提出來。 在進行30 kDa分離後，以崖咖⑧·_15蛋白質濃縮過滤器 (Millipore) PL-10進行分離(1〇池分離)，以醋酸納緩衝液(5〇碰， 酸驗值5.5)洗提純化之米根黴_殿粉酶/黑麵菌葡糖搬粉酶。 實施例二米根徽葡糖澱粉酶之醣晶片分析 同先前所述’ 大腸桿難絲現絲pET23a製造絲黴葡糖 救粉酶重組蛋自，伽直鏈驗樹脂進行·。由雜歸質體學 協會’核心Η設施進行醣晶片分析。此晶片包含總共377種不同之 自然及合成多®I，並使用版本3>1來進行分析。簡而言之，以醋酸 鈉結合緩衝液(5GmM醋酸納，酸驗值5 5，1%牛血清蛋自，〇〇5% 聚山梨醇醋20)將米根黴葡糖酶重組蛋白稀釋至勘毫克/毫 升、。將70微升之稀釋液鋪在晶片表面，蓋上玻片，在冑溫避光的 情況下於麵器中培養-小時。培養過後，移除蓋玻片，並以TSM 緩衝液(50 mM三羥甲基氨基甲烷_鹽酸，酸驗值7 5，％祕氣化 鎮以及0.5 Μ嚴糖)清洗4次。以1:2〇〇之倍數以碟酸鹽緩衝液結合 緩衝液稀釋70微升的抗米根黴葡糖殿粉酶之單株抗體，並將_ 2在晶片表面’在_器巾培養L清洗方法如先前所述。 ^測結合程度，使用綱!_緩衝液結合緩衝液稀釋至5毫克/ Α_88•標記山羊抗小鼠免疫球蛋白g進行二級抗體培養， ff·片置缝·中—小時，再料前所述之方法輕洗。利用 Mcm-Ehner微陣列XL侧掃描器讀取結合影像並使用 Imagene(V.6)影像分析軟體進行分析。 201040280 圖一 A顯示米根黴葡糖澱粉酶之醣晶片分析結果。在377種多醣 中，只有 Mana(l，2) Manct(l，2) Mana(l，3) Mana(No. 187)產生明顯的 交互作用訊號峰。圖一 B顯示具有最強信號之特殊的Mana(l，2) Mana(l，2) Mana(l，3) Mana之分子結構。根據由功能性醣質體學協 會核心Η設施提供之分析數據，大部分具有Mana(12)
Mana(l，3) Mana結構之人類相關蛋白質屬於人類c 型凝集素家族’例如朗格素(Langerin)(PDB ID:3BC7)、甘露糖結合 蛋白/凝集素(PDB ID: 1HUP)以及肺泡表面蛋白質proteins) D (PDB ID:1PWB)。另外，一個特殊的fflV1中和抗體2G12也被發 現具有辨認 HIV1 醣蛋白 120 之 Mana(l，2) Mana(l，2) Manc<l，3) Mana 結構的特徵(Wang JS，et al·, Org. Biomol. Chem. (2007) 5: 1529-1540 and PashovA，et al” Glycobiology (2005) 15: 994-1001)。因 此，本發明利用三明治酵素免疫分析法，測試米根徽葡糖搬粉酶結 合在HIV抗原&抗體陽性組中之jjjy自然抗原之能力。 三迎2重键變«舆米根微葡賊粉酶之結構棋擬及序
圖二入顯示犯12人類單株抗體重鏈變異區，左）及 米根黴葡糖澱粉酶(PBD取2複，右)之結構模擬結果。重鍵變異 之重鍵’並與N鍵結聽基結合。綱及米根黴葡糖鞭 ^構之重細二B)，顯示兩蛋白質之二級結構貝它褶板ί 結合殘基餘她之杨(結猶照之均方 移值為2.43 A)。米根徽葡糖激粉酶也同樣被證實.般抗體具 9 201040280 實施例四HIV-米根黴葡糖澱粉酶結合分析 製備塗鑛米根黴葡糖澱粉酶/黑麴菌葡糖殿粉酶之培養皿以進行酵 素免疫分析法 首先在 96 孔培養皿(Greiner-Bio One GmbH, Frickenhausen，德國) 之個別孔中，加入100微升碳酸氫鈉緩衝液(酸鹼值95)/三羥甲基 氨基甲烧-鹽酸(酸鹼值8.0)之米根黴葡糖澱粉酶/黑麴菌葡糖澱粉 酶，並於4 T培養16小時，使之鍵於各孔盤内。以磷酸鹽緩衝液 (10 mM磷酸鹽緩衝液(酸鹼值7.〇)加上〇_05%聚山梨醇酯2〇)清洗培 養皿，在每個孔中加入200微升之阻隔緩衝液(0.01 M磷酸鹽緩衝 液(酸驗值7.0)加上5%牛血_清蛋白）’並於37 °c培養2小時。移除 阻隔緩衝液’並在25 °C乾燥培養皿1小時。每個孔分別加入1〇〇 微升之抗原&抗體陽性組(編號9144532，SeraCare生命科學， Milford，MA)或抗HCV混合濃度表現組(編號PHV205-24，SeraCare 生命科學，Milford，ΜΑ)，並於37 〇c反應1小時。聰抗原&抗 體陽性組經由Perkin Elmer之酵素免疫偵測試劑確認為hjV抗原陽 性’並經由Abbott之酵素免疫偵測試劑確認為抗mv抗體陽性。 接下來，加入辣根過氧化氫酶結合大腸桿菌重組蛋白之HJV1酶蛋 白120抗原(0.05微克/毫升）’於37 T反應30分鐘。此結合物可直 接辨識HIV抗原&抗體陽性組中之人類抗HIV抗體。為測試葡糖澱 粉酶結合HIV之專一性，以相似方式分析HCV試驗檢體及辣根過 氧化氫酶結合HCV之重組抗原(核心蛋白+非結構性蛋白3+非結構 性蛋白5)(0.33微克/毫升）。最後，加入1〇〇微升之3,3，,5,5'-四甲基 聯苯胺(TMB)至培養皿中，並於37 T培養30分鐘。在每個孔加入 1〇〇微升之2N硫酸以終止反應，並利用酵素免疫分析儀在45〇啦 測量吸光值。將這些吸光質轉換為一個統計值(閥值，cut〇ffvalue， COV)，並以截止指數(cut 0ff index，COI)表示。閥值的計算係將負 201040280 控制(正常人類血清)之吸光值加01(閥值=負控制+〇1);截止指數 的叶算則是將待測檢體之吸光值除以閥值。若一個檢體的吸光質高 於閱值，舉例而言’其截止指數大於1，則此檢驗結果即視為陽性。 圖四A顯示，米根黴葡糖澱粉酶結合之最大截止指數為 2·298(Ηΐν)。另外’圖四B使用抗HCV混合濃度表現組以顯示米 I黴葡糖殿粉酶之專-性。其差料有麟顯雜(p<().⑻叫。這 些結果指出米根黴葡糖澱粉酶對ιπν抗原&抗體陽性組具有專一性 0 結合，可能是與HTV之抗原醣蛋白120反應。 再者，利用HIV1發生率/流行率表現組PRB6〇1(SeraCare生命科 學’ Milford ’ MA)為待測檢體’味其在塗鑛米根黴葡糖殿粉酶之 酵素免疫分析法和Coulter HIV-1 p24抗原試驗中之表現，或比較其 在黑麴菌葡糖澱粉酶之酵素免疫分析和Couhrpjjyq ρ24抗原試驗 中之表現。相關檢體係取自美國聰陽性捐血者之未稀釋單位血 聚，但捐血者之感染及血清轉換日期未知。這些檢體都經過c咖把 抗HIV1西方墨點法檢測，結果顯示15個檢艘皆為抗抓^ p〗4和 抗膽蛋自160抗體陽性。圖五A和圖五丑顯示，傳統HIVip24抗 U 原試驗之偵測率僅有2〇應，相較之下，塗鍍米根黴葡糖澱粉酶之 酵素免疫分析法(93.3%)及塗舰麴_健_之酵素免疫分析 &(867%)H貞測率高出許多。 實施例五ϋ蛋白對米根撤葡糖狹粉峰/黑典菌葡糖殿粉蘇結合 HIV之影響 將 10 mM 麥芽七糖((}7 glycan)和環糊精(p_CD)(sigma Aidrich，聖路 易’ MO)溶解在含有5%牛血清蛋自之碟酸鹽緩衝液，並將其與同樣 體積之HIV抗原&抗體·組混合，於π %反應丨小時。將混合 液加入塗齡細:雜絲酶之培“(在Q Q5 M三㈣基氨基甲 201040280 院-鹽酸中為100 nM，酸鹼值8.0)或加入塗鍍黑麴菌葡糖殿粉酶之 之培養皿(在0.05 Μ碳酸氫鈉緩衝液中為100 nM，酸鹼值9 5)後， 經37 °C反應1小時，加入辣根過氧化氫酶結合大腸桿菌重組蛋白 之HIV1醣蛋白12〇抗原(〇.〇5微克/毫升)100微升以進行偵測。 圖六A顯示mV抗原&抗體陽性組之截止指數在麥芽七糖和環糊精 存在的情況會減少。將10 mM麥芽七糖或環糊精加入塗鍍米根黴葡 糖澱粉酶之培養皿’會出現競爭現象，強烈指出麥芽七糖和環糊精 為米根黴葡糖澱粉酶和HIV抗原&抗體陽性組的結合作用之競爭 者’其中10 mM麥芽七糖和環糊精之競爭抑制率分別為24.4%和 23.1%。 相同地，競爭現象發生在將10 mM麥芽七糖或環糊精加入塗鑛黑麴 菌葡糖澱粉酶之培養皿（圖六B) ’顯示麥芽七糖和環糊精在黑麴菌 葡糖澱粉酶和HIV抗原&抗體陽性組的結合作用亦為競爭者。1〇 應麥芽七糖對黑麴菌葡糖澱粉酶之競爭抑制率為45.9%，較其對 米根黴葡糖殿粉酶之競爭抑制率為高。然而10 mM環糊精對黑麵菌 葡糖澱粉酶之競爭抑制率(14.3%)，則較其對米根黴葡糖殿粉酶之競 爭抑制率為低。 實施例六2G12和醣蛋白140對HIV-米根擻葡糖澱粉酶和HIV_ 黑麴菌葡糖澱粉酶結合之競爭作用 將HIV抗原&抗體陽性組分別混合與相同體積之500 nM人類免疫 球蛋白G!二級抗體、500 nM 2G12單株抗體或250 nM HIV1醣蛋 白140抗原，於37 °C作用1小時。將混合液加入塗鍍米根黴葡糖 澱粉酶之培養皿(在0.05M三羥甲基氨基甲烷-鹽酸中為100 nM，酸 驗值8.0 )或加入塗鑛黑麴菌葡糖澱粉酶之之培養皿(在〇.〇5 Μ碳酸 氫鈉緩衝液中為1〇〇 nM，酸鹼值9.5 )後，經37。(：反應1小時，加 12 201040280 入100微升之辣根過氧化氫酶結合大腸桿菌重組蛋白之HJV1聽蛋 白120抗原(0.05微克/毫升)以進行偵測。 圖七A和圖七B顯示ffiV抗原&抗體陽性組之截止指數在2G12單 株抗體存在的情況會減少現象。將500nM2G12單株抗體精加入塗 鍍米根黴葡糖澱粉酶之培養皿(圖七A)和塗鍍黑麴菌葡糖殿粉酶之 培養皿(圖七B)，會出現競爭現象。這些結果指出，相較於人類免 疫球蛋白& ’ 2G12出現專一性的競爭現象。其中5〇〇nM2G12對 米根黴葡糖澱粉酶之競爭抑制率為28.9%，對黑麴菌葡糖澱粉酶之 競爭抑制率則為28.4%。相反地，500 nM人類免疫球蛋白q並不 造成任何競爭現象。另外，250nMHIVl醣蛋白140的存在之競爭 作用更為明顯’其抑制率分別為對米根黴葡糖澱粉酶為429%和對 黑麴菌葡糖澱粉酶為40.1%。這些結果強烈顯示澱粉結合區域和 HIV醣蛋白120之間的專一,丨生相互作用。 實施例七HIV1分離株之2G12抗原決定位之胺基酸序列排比 圖八A和圖八B顯示21個HIV1分離株(SEQ ID NO:4 to SEQ ID 〇 NO:23)之2G12人類單株抗體抗原決定位之多重胺基酸序列排比 (SEQIDNO:3)〇2G12抗原決定位主要包含在HTV1IIIB醣蛋白120 和JR-FL分離株上之多量甘露醣或兩種混合醣殘基天門冬胺酸 295，天門冬胺酸332，天門冬胺酸339，天門冬胺酸386，天門冬 胺酸 392 及天門冬胺酸 448(Sanders RW, et al.，J. Virol. (2002) 76: 7293-7305 and Scanlan CN，et al” J. Virol. (2002) 76: 7306-7321)。這 些天門冬胺酸殘基在21個HIV1分離株之醣蛋白12〇序列中，皆明 顯地高度保留。圖七A和圖七B中之6個N連結醣基化位置，位 於底線標示之天門冬胺酸1 ’天門冬胺酸38，天門冬胺酸92，天門 冬胺酸98，天Π冬胺酸103及天門冬胺酸154，符合2G12辨識位。 另外’天門冬胺酸38和天門冬胺酸98被HIV模組辨認為高度甘露 13 201040280 麵識位(Calarese DA，et al·，Science (2〇〇3) 3〇〇:如仍-期)。這些始 果指出，雖然HIV具有快速遺傳漂變之特性，隨時間推移，其N 連結醣基餘置較穩衫變，使得這齡置讀殊醣類成為 極具潛力的專-性辨識標的。_結合模組調控之贿辨識區域^ 作為-個抗體機触_ Ηΐν ’並針騎泛變制聊品系感 染，發展預防及治療試劑。 【圖式簡單說明】 圖-A及圖-B細晶片分析決定米纖（施_—從）葡糖 澱粉酶（i^SBD)之多醣辨識。 圖二八係灿2人類單株抗體重鏈變異區(pDB聊耶，左）及米 根黴葡糖搬粉酶(PBD ID:2V8M，右)之模擬結果。圖二B係2G12 及米根黴葡糖殿粉酶結構之重疊。 囷三係2G12人類單株抗體(PBD取⑽)重鏈變異區和米根徽葡 糖殿粉酶咖SBD)(PBD ID:2V8M)之胺基酸序列排比，僅有8 9%的 相似磨。 圖四A、圖四B及圖四C係侧卿抗原&抗體編號9144532陽性 組和米根黴葡糖澱粉酶的結合。 ，五A係·腿發生键行率表雜pRB和絲徽葡糖殿 叙酶的結合；圖五B係利用職發生率/流行率表現組pRB6〇i和 黑麴菌(Αρπ㈣/泌吨㈣之葡糖澱粉酶(如SBD)的結合。 圖六A係米雛葡槪殿粉酶與麥芽域㈣和環糊精(p_cD)結合 HIV之間的競爭，圖六B係黑麴菌葡糖殺粉酶與麥芽七糖仰)和環 201040280 糊精(β- CD)結合上HIV之間的競爭。 圖七A係米根黴葡糖澱粉酶與HIV1醣蛋白140抗原和2G12單株 抗體結合HIV之間的競爭；圖七B係黑麴菌葡糖殿粉酶與mV1醣 蛋白140抗原和2G12單株抗體結合_之間的競爭。 圖八A及圖八B係Ή個HIV1分離株之2gi2抗原決定位之胺基酸 序列排比。 〇 【主要元件符號說明】 無 〇 15 201040280 序列表 <110>國立清華大學 <120>醣類結合模組及其應用 <130> 0885-NTHU-US <160> 24 <170> Patentln version 3.4 <210> 1 <211> 123 <212> PRT <213> Artificial <220> <223> VH domain of 2G12 antibody <220> <221> DOMAIN <222> (1)..(123) <400> 1
Glu Val Gin Leu Val 6lu Ser Gly Gly Gly Leu Val Lys Ala Gly Gly 15 10 15
Ser Leu lie Leu Ser Cys Gly Val Ser Asn Phe Arg Ile Ser Ala His 20 25 30
Thr Met Asn Trp Val Arg Arg Val Pro Gly Gly Gly Leu Glu Trp Val 35 40 45
Ala Ser Ile Ser Thr Ser Ser Thr Tyr Arg Asp Tyr Ala Asp Ala Val 50 55 60 16 201040280
Lys Gly Arg Phe Thr Val Ser Arg Asp Asp Leu Glu Asp Phe Val Tyr 65 70 75 80
Leu Gin Met His Lys Met Arg Val Glu Asp Thr Ala Ile Tyr Tyr Cys 85 90 95
Ala Arg Lys Gly Ser Asp Arg Leu Ser Asp Asn Asp Pro Phe Asp Ala 100 105 110
Trp Gly Pro Gly Thr Val Val Thr Val Ser Pro 115 120 o
<210> 2 <211> 106 <212> PRT <213> Rhizopus oryzae <220> <221> DOMAIN <222> (1)..(106)
<400> 2
Ala Ser lie Pro Ser Ser Ala Ser Val Gin Leu Asp Ser Tyr Asn Tyr 15 10 15
Asp Gly Ser Thr Phe Ser Gly Lys lie Tyr Val Lys Asn lie Ala Tyr 20 25 30
Ser Lys Lys Val Thr Val Val Tyr Ala Asp Gly Ser Asp Asn Trp Asn 35 40 45
Asn Asn Gly Asn lie lie Ala Ala Ser Phe Ser Gly Pro I le Ser Gly 50 55 60 17 201040280
Ser Asn Tyr Glu Tyr Trp Thr Phe Ser Ala Ser Val Lys Gly lie Lys 65 70 75 80
Glu Phe Tyr lie Lys Tyr Glu Val Ser Gly Lys Thr Tyr Tyr Asp Asn 85 90 95
Asn Asn Ser Ala Asn Tyr Gin Val Ser Thr 100 105 <210> 3 <211> 154 <212> PRT <213> Artificial <220> <223> antibody <220> <221> DOMAIN <222> (1)..(154) <400> 3
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Arg Ile Arg Ile Gin 15 10 15
Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys Ile Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Asn Thr Leu 35 40 45
Lys Gin Ile Ala Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60 lie Ile Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 18 201040280 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg lie Lys Gin 115 120 125
lie lie Asn Met Trp Gin Lys Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140
Ile Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150
<210> 4 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 <220> <221> MISC—FEATURE <222> (1)..(154) <223> HXB2 <400> 4
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Arg Ile Arg lie Gin 15 10 15
Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys Ile Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Asn Thr Leu 35 40 45 19 201040280
Lys Gin IΙθ Ala Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60 lie Ile Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Me Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg Ile Lys Gin 115 120 125
Me lie Asn Met Trp Gin Lys Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140 lie Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150
<210> 5 <211> 154 <212> PRT <213> Human immunodef iciency virus type 1 <220> <221> MISC—FEATURE <222> (1)..(154) <223> LW852 <400> 5
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Arg Me Arg I le Gin 15 10 15 20 201040280
Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys Ile Gly Asn Met 2〇 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Asn Thr Leu 35 40 45
Lys Gin Ile Ala Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60
He lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu lie Val Thr His 65 70 75 80
o
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg lie Lys Gin 115 120 125 lie Me Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140
Ile Ser Gly Gin Ile Arg Cys Ser Ser Asn 145 150
<210 6 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 <220> <221> MISC_FEATURE <222> (1)..(154) <223> wt1 21 201040280 <400> 6
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Lys Ile Arg lie Gin 15 10 15
Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys lie 6ly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu 35 40 45
Lys Gin Ile Ala Ser Lys Leu Arg 6lu Gin Phe Gly Asn Asn Lys Thr 50 55 60 lie lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg Ile Lys Gin 115 120 125 lie lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140 lie Ser Gly 6ln lie Arg Cys Ser Ser Asn 145 150
<210> 7 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 22 201040280 <220> <221> MISC_FEATURE <222> (1)..(154) <223> MCK1 <400> 7
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Arg Ile Arg Ile Gin 15 10 15
o
Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys Ile Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu 35 40 45
Lys Gin lie Ala Ser Lys Leu Arg 6lu Gin Phe Gly Asn Asn Lys Thr 50 55 60 lie lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg lie Lys Gin 115 120 125
Phe Ile Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140 lie Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150 23 201040280
<210> 8 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 <220> <221> MISC_FEATURE <222> (1)..(154) <223> CH69-2 <400> 8
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Lys Ile Arg lie Gin 15 10 15
Arg Gly Pro Gly Arg Ala Phe Val Thr lie Gly Lys lie Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu 35 40 45
Lys Gin Ile Ala Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60 lie lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Th「 Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg lie Lys Gin 115 120 125 24 201040280 lie lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140
Ile Ser Gly Gin Ile Arg Cys Ser Ser Asn 145 150 <210> 9 <211> 154 <212> PRT
<213> Human immunodeficiency virus type 1 <220> <221> MISC_FEATURE <222> (1)..(154) <223> XB3 <400> 9
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Lys Ile Arg Ile Gin 1 5 10 15
Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys Ile Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu 35 40 45
Lys Gin Ile Ala Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60 lie Ile Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95 25 201040280
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu 61y Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg lie Lys Gin 115 120 125
Phe Ile Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140
Ile Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150 <210> 10 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 <220> <221> MISC_FEATURE <222> (1)..(154) <223> wt2 <400> 10
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Lys Ile Arg lie Gin 15 10 15
Arg Gly Pro Gly Arg Ala Phe Val Thr lie Gly Lys Me Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn iie Ser Arg Aia Lys Trp Asn Ala Thr Leu 35 40 45
Lys Gin lie Asp Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60 26 201040280 lie lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg Ile Lys Gin 115 120 125 o
Me lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140 lie Ser Gly Gin Me Arg Cys Ser Ser Asn 145 150
<210> 11 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 o <220> <221> MISC_FEATURE <222> (1)..(154) <223> LW12.3 <400> 11
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Arg Ile Arg lie Gin 15 10 15
Arg Gly Pro Gly Arg Thr Phe Val Thr Ile Gly Lys Ile Gly Asn Met 20 25 30 27 201040280
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Asn Thr Leu 35 40 45
Lys Gin lie Ala Ser Lys Leu Arg Glu Gin Tyr Gly Asn Asn Lys Thr 50 55 60 lie Ile Phe Lys Gin Ser Ser Gly Gly Asp Leu Glu Ile Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg Ile Lys Gin 115 120 125 lie lie Asn Met Trp Gin Glu Va! Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140
Ile Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150
<210> 12 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 <220> <221> MISC_FEATURE <222> (1)..(154) <223> BH10 <400> 12 28 201040280
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Me Arg lie Gin 15 10 15
Arg Gly Pro Gly Arg Ala Phe Val Thr lie Gly Lys lie Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Asn Thr Leu 35 40 45
Lys Gin lie Asp Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr
Me lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Me Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Lys Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg Ile Lys Gin 115 120 125
G lie lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140
Ile Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150
<210 13 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 29 201040280 <220> <221> MISC_FEATURE <222> (1)..(154) <223> PV22 <400> 13
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Me Arg Ile Gin 1 5 10 15
Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys Ile Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Asn Thr Leu 35 40 45
Lys Gin Ile Asp Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60 lie lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg Ile Lys Gin 115 120 125
Phe lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140 lie Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150 30 201040280
<210> 14 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 <220> <221> MISC_FEATURE <222> (1)..(154)
<223> BRU <400> 14 ❹
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg I le Gin 15 10 15
Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys Ile Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu 35 40 45
Lys Gin Ile Ala Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60 〇 lie Ile Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg lie Lys Gin 115 120 125
Phe Ile Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 31 201040280 130 135 140
Ile Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150 <210> 15 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 <220> <221> MISC_FEATURE <222> (1)..(154) <223> CH69-7 <400> 15
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Lys Me Arg Ile Gin 15 10 15
Arg Gly Pro Gly Arg Ala Phe Val Thr Leu Gly Lys Ile Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu 35 40 45
Lys Gin lie Ala Ser Lys Leu Arg Glu Gin Phe Gly Ser Asn Lys Thr 50 55 60 lie lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 32 201040280 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg lie Lys Gin 115 120 125 lie lie Asn Met Trp Gin Giu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140 lie Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150
<210> 16 <211> 154 <212> PRT <213> Human immunodef iciency virus type 1 <220〉 <221> MISC_FEATURE <222> (1)..(154) <223> 1H6 <400> 16
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Lys lie Arg lie Gin 15 10 15
Arg Gly Pro Gly Arg Thr Phe Val Thr Ile Gly Lys Ile Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn lie Ser Arg Ala Lys Trp Asn Ala Thr Leu 35 40 45
Lys Gin lie Asp Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60 lie lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 33 201040280 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg Ile Lys Gin 115 120 125 lie lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140
Ile Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150
<210> 17 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 <220> <221> Ml SC—FEATURE <222> (1)..(154) <223> 1F8 <400> 17
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Lys Ile Arg lie Gin 15 10 15
Arg Gly Pro Gly Arg Ala Leu Val Thr lie Gly Lys lie Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu 34 201040280 35 40 45
Lys Gin lie Asp Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60 lie lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg Ile Lys Gin 115 120 125 lie lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140 lie Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150
<210> 18 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 <220>
<221> MISC_FEATURE <222> (1)..(154) <223> mfA <400> 18 35 201040280
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Ser Ile Arg Ile Gin 15 10 15
Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys Ile Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn lie Ser Arg Ala Lys Trp Met Ser Thr Leu 35 40 45
Lys Gin Ile Ala Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60
Val Ile Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg Ile Lys Gin 115 120 125
Phe lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140
Ile Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150
<210> 19 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 36 <220> 201040280 <221> MISC_FEATURE <222> (1)..(154) <223> CH69-6 <400> 19
Asn Cys Ile Arg Pro Asn Asn Asn Thr Arg Lys Lys Ile Arg lie Gin 15 10 15
Arg Gly Pro Gly Arg Ala Leu Val Thr Ile Gly Lys Ile Gly Asn Met 20 25 30 O Arg Gin Ala His Cys Asn lie Ser Arg Ala Lys Trp Asn Ala Thr Leu 35 40 45
Lys Gin lie Ala Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60 lie lie Phe Lys Gtn Ser Ser Gly Gly Asp Pro Glu Ile Va! Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Ala Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg lie Lys Gin 115 120 125 lie lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140
Ile Ser Gly Gin Ile Arg Cys Ser Ser Asn 145 150 <210> 20 37 201040280
<211> 154 <212> PRT <213> Human immunodeficiency virus type 1 <220> <221> MISC_FEATURE <222> (1)..(154) <223> CH69-5 <400> 20
Asn Cys lie Arg Pro Asn Asn Asn Thr Arg Lys Lys Me Arg Ile Gin 15 10 15
Arg Gly Pro Gly Arg Ala Leu Val Thr Ile Gly Lys Ile Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu 35 40 45
Lys Gin Ile Ala Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60
Me lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu lie Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Ala Glu Gly Ser Asp Thr Me Thr Leu Pro Cys Arg Me Lys G!n 115 120 125
Me lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140 38 201040280
lie Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150 <210> 21 <211> 154 <212> PRT <213> Human immunodef iciency virus type 1
<220> <221> MISC_FEATURE <222> (1)..(154) <223> 1B6 <400〉 21
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Lys Ile Arg Ile Gin 1 5 10 15
Arg Gly Pro Gly Arg Thr Phe Asp Thr Me Gly Lys lie Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn lie Ser Arg Ala Lys Trp Asn Ala Thr Leu 35 40 45
Lys Gin lie Asp Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60 lie lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110 39 201040280
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg Ile Lys Gin 115 120 125 lie lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140
lie Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150 <210> 22 <211> 154 <212> PRT <213> Human immunodeficiency virus type 1 <220> <221> Ml SC—FEATURE <222> (1)..(154) <223> CH69-1 <400> 22
Asn Cys Ala Arg Pro Asn Asn Asn Thr Arg Lys Lys Ile Arg lie Arg 15 10 15
Arg Gly Pro Gly Arg Ala Phe Val Thr Ile Gly Lys Ile Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu 35 40 45
Lys Gin i ie Aia Ser Lys Leu Arg Glu Gin Phe Giy Asn Asn Lys Thr 50 55 60 lie lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu Ile Val Ala His 65 70 75 80 201040280
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg Ile Lys Gin 115 120 125 lie lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro _ 130 135 140 o
lie Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150 <210> 23 <211> 154 <212> PRT <213> Human immunodef iciency virus type 1 <220>
<221> MISC_FEATURE <222> (1)..(154) <223> TH4-7-5 <400> 23
Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg Lys Lys Phe Arg lie Gin 15 10 15
Arg Gly Pro Gly Arg Ala Phe Val Thr lie Gly Lys lie Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu 35 40 45 41 201040280
Lys Gin Ile Ala Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60 lie lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu lie Val Thr His 65 70 75 80
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Ser Asn Ser Thr Leu Ser Thr Glu Gly Ser Asn 100 105 110
Asn Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg Me Lys Gin 115 120 125
Me lie Asn Met Trp Gin Glu Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140 I le Ser Gly Gin Me Arg Cys Ser Ser Asn 145 150
<210> 24 <211> 154 <212> PRT <213> Human immunodef iciency virus type 1 <220> <221> MiSC_FEATURE <222> (1)..(154) <223> Human immunodeficiency virus type 1 envelope glycoprotein gp160 gene <400> 24
Asn Cys Thr Arg Pro Tyr Asn Asn Lys Arg Lys Ser lie Arg lie Gin 15 10 15 42 201040280
Arg Gly Pro Gly Arg Ala Phe Val Thr lie Gly Lys lie Gly Asn Met 20 25 30
Arg Gin Ala His Cys Asn Ile Ser Arg Ala Lys Trp Asn Ala Thr Leu 35 40 45
Lys Gin lie Ala Ser Lys Leu Arg Glu Gin Phe Gly Asn Asn Lys Thr 50 55 60 lie lie Phe Lys Gin Ser Ser Gly Gly Asp Pro Glu lie Val Thr His ^ 65 70 75 80 o
Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Ser Thr Gin Leu 85 90 95
Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp Ser Thr Glu Glu Ser Asn 100 105 110
Ser Thr Glu Gly Ser Asp Thr lie Thr Leu Pro Cys Arg lie Lys Gin 115 120 125
Phe lie Asn Met Trp Gin Lys Val Gly Lys Ala Met Tyr Ala Pro Pro 130 135 140
G lie Ser Gly Gin lie Arg Cys Ser Ser Asn 145 150 43
Claims (1)
- 201040280 七、申請專利範圍： 1. 一種醣類結合模組之抗體模擬物，其可特異性結合jjjy醣蛋白的一 個抗原決定位。 2. 根據申請專利範圍第1項的抗體模擬物，其中醣類結合模組可特異性 結合HIV畴蛋白之抗原決定位的一個多聽結構。 3. 根據申請專利範圍第丨項的抗體模擬物，其中醣類結合模組含有類似 標準抗體之類免疫球蛋白（Ig_like)區域。 4·根據申請專利範圍第1項的抗體模擬物，其中醣類結合模組係澱粉結 合區域。 5. 根據申請專利範圍第4項的抗體模擬物，其中澱粉結合區域係選自醣 類結合模組家族第20、21、25、26、34、41、45、48和53族中的 一員。 6. 根據申請專利範圍第5項的抗體模擬物，其中澱粉結合區域係醣類結 合模組家族第20族。 7·根據申請專利範圍第5項的抗體模擬物，其中澱粉結合區域係醣類結 合模組家族第21族。 8. 根據申請專利範圍帛5項的抗體模擬物，其中澱粉結合區域係米根黴 之葡糖漱粉酶(及。 9. 根據申Μ專鄕㈣5棚抗體模擬物，其巾麟結合區域係黑麵菌 44 201040280 傾7/沿吨之葡糖澱粉酶(如SBD)。 10.—種偵測mv醣蛋白的方法，包含： 採用口體表面’其上結合能與醣蛋白結合的已知量之醣類結 合模組； Ο將樣本與上述表面接觸，以培養形成_結合模組醣蛋白複 合體；以及 。養此複^體’並加人—個能產生可量化·之減的標記服 原、。口體’ H預樣本為抓絲陽性或陰性。 U_根據申糊範_ 1G項的方法，財步驟e陳抗原，標記 素I酵素在接觸—個酵素基質時能夠產生上述之訊號。 12·根據申請專利範圍第10項的方法，其中聽類結合模組係澱粉結合區 域。 據申請專利|&圍第u項的方法，其中雜結合區域係米纖葡糖 澱粉酶。 申請專利域第12項的方法，其中澱粉結合區域係黑麴菌葡糖 澱粉酶。 45
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/464,788 US20100291540A1 (en) | 2009-05-12 | 2009-05-12 | Carbohydrate binding module and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW201040280A true TW201040280A (en) | 2010-11-16 |
| TWI391491B TWI391491B (zh) | 2013-04-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| TW098124661A TWI391491B (zh) | 2009-05-12 | 2009-07-22 | 醣類結合模組及其應用 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US20100291540A1 (zh) |
| EP (1) | EP2430045B1 (zh) |
| JP (1) | JP5746149B2 (zh) |
| CN (1) | CN103097411B (zh) |
| TW (1) | TWI391491B (zh) |
| WO (1) | WO2010131114A2 (zh) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016029220A1 (en) * | 2014-08-22 | 2016-02-25 | Nectagen, Inc. | Affinity proteins and uses thereof |
| CN105219753B (zh) * | 2015-11-03 | 2018-11-02 | 绍兴加华生物科技有限公司 | 一种固定化的有机磷农药降解酶及其制备方法与应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| AU2003212459B2 (en) * | 2002-02-25 | 2008-05-08 | Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Service | An obligate domain-swapped dimer of cyanovirin with enhanced anti-viral activity |
| AU2003267356A1 (en) * | 2002-10-01 | 2004-04-23 | Unilever Plc | Proteins having polysaccharidase activity |
| US7662918B2 (en) * | 2005-03-03 | 2010-02-16 | Simpson Biotech Co., Ltd. | Recombinant protein comprising starch binding domain and use thereof |
| US8202523B2 (en) * | 2005-09-22 | 2012-06-19 | ProSci, Inc. | Glycosylated polypeptides produced in yeast mutants and methods of use thereof |
| US8865876B2 (en) * | 2008-06-02 | 2014-10-21 | California Institute Of Technology | Engineered lectin oligomers with antiviral activity |
-
2009
- 2009-05-12 US US12/464,788 patent/US20100291540A1/en not_active Abandoned
- 2009-07-22 TW TW098124661A patent/TWI391491B/zh not_active IP Right Cessation
-
2010
- 2010-04-06 US US12/755,242 patent/US8293465B2/en not_active Expired - Fee Related
- 2010-05-12 JP JP2012510383A patent/JP5746149B2/ja not_active Expired - Fee Related
- 2010-05-12 EP EP10774609.1A patent/EP2430045B1/en not_active Not-in-force
- 2010-05-12 CN CN201080021113.XA patent/CN103097411B/zh not_active Expired - Fee Related
- 2010-05-12 WO PCT/IB2010/001302 patent/WO2010131114A2/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2010131114A2 (en) | 2010-11-18 |
| CN103097411A (zh) | 2013-05-08 |
| JP5746149B2 (ja) | 2015-07-08 |
| WO2010131114A3 (en) | 2011-11-24 |
| JP2012529426A (ja) | 2012-11-22 |
| US20100291540A1 (en) | 2010-11-18 |
| EP2430045A4 (en) | 2013-02-13 |
| US20100291601A1 (en) | 2010-11-18 |
| EP2430045B1 (en) | 2015-07-08 |
| TWI391491B (zh) | 2013-04-01 |
| US8293465B2 (en) | 2012-10-23 |
| EP2430045A2 (en) | 2012-03-21 |
| CN103097411B (zh) | 2016-09-28 |
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