TR200002624T2 - Bitkilerdeki trehaloz biyosentetik genlerin ekspresyonu - Google Patents

Bitkilerdeki trehaloz biyosentetik genlerin ekspresyonu

Info

Publication number
TR200002624T2
TR200002624T2 TR2000/02624T TR200002624T TR200002624T2 TR 200002624 T2 TR200002624 T2 TR 200002624T2 TR 2000/02624 T TR2000/02624 T TR 2000/02624T TR 200002624 T TR200002624 T TR 200002624T TR 200002624 T2 TR200002624 T2 TR 200002624T2
Authority
TR
Turkey
Prior art keywords
expression
trehalose biosynthetic
plants
biosynthetic genes
trehalose
Prior art date
Application number
TR2000/02624T
Other languages
English (en)
Turkish (tr)
Inventor
Guillaume Lebel Edouard
Bernard Heifetz Peter
Arthur Goff Stephen
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of TR200002624T2 publication Critical patent/TR200002624T2/xx

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
TR2000/02624T 1998-03-11 1999-03-09 Bitkilerdeki trehaloz biyosentetik genlerin ekspresyonu TR200002624T2 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US7766598P 1998-03-11 1998-03-11

Publications (1)

Publication Number Publication Date
TR200002624T2 true TR200002624T2 (tr) 2000-11-21

Family

ID=22139381

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2000/02624T TR200002624T2 (tr) 1998-03-11 1999-03-09 Bitkilerdeki trehaloz biyosentetik genlerin ekspresyonu

Country Status (15)

Country Link
US (2) US6686516B2 (https=)
EP (1) EP1064365A2 (https=)
JP (1) JP2002505875A (https=)
KR (1) KR20010082520A (https=)
CN (1) CN1292824A (https=)
AU (1) AU748604B2 (https=)
BR (1) BR9909258A (https=)
CA (1) CA2320896A1 (https=)
EA (1) EA200000926A1 (https=)
HU (1) HUP0102598A3 (https=)
IL (1) IL138191A0 (https=)
IN (1) IN2000CN00368A (https=)
PL (1) PL343460A1 (https=)
TR (1) TR200002624T2 (https=)
WO (1) WO1999046370A2 (https=)

Families Citing this family (34)

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US7214858B2 (en) * 1999-03-31 2007-05-08 E. I. Du Pont De Nemours And Company Plant genes encoding trehalose metabolism enzymes
WO2001007590A2 (en) * 1999-07-27 2001-02-01 Syngenta Participations Ag Chimeric genes for plastid expression
CN100425701C (zh) 1999-12-16 2008-10-15 孟山都技术有限公司 新型植物表达构建物
WO2001064850A1 (en) * 2000-02-29 2001-09-07 Auburn University Genetic engineering of drought tolerance via a plastid genome
US20040064846A1 (en) * 2001-02-28 2004-04-01 Henry Daniell Genetic engineering of drought tolerance via a plastid genome
US20040014174A1 (en) * 2002-04-23 2004-01-22 Mayfield Stephen P. Expression of polypeptides in chloroplasts, and compositions and methods for expressing same
EP1375669A1 (en) * 2002-06-13 2004-01-02 Stichting Voor De Technische Wetenschappen Method for enhancing the disease resistance in plants by altering trehalose-6-phosphate levels
KR100440725B1 (ko) * 2002-06-20 2004-07-15 주식회사 그린진 바이오텍 비생물성 스트레스에 대한 단자엽 식물의 내성을증가시키는 방법
US20040187175A1 (en) * 2002-11-06 2004-09-23 Wu Ray J. TPS plant gene constructs and transformants
WO2004044148A2 (en) * 2002-11-08 2004-05-27 Paradigm Genetics, Inc. Methods for the identification of trehalose-6-phosphate synthase as antibiotics
CN101023175B (zh) * 2004-04-20 2012-02-29 辛根塔参与股份公司 用于在植物繁殖组织中表达基因产物的调控序列
WO2006060376A2 (en) * 2004-12-03 2006-06-08 Syngenta Participations Ag Stress tolerance in plants through selective inhibition of trehalose-6-phosphate phosphatase
CN100355883C (zh) * 2004-12-10 2007-12-19 中国科学院上海生命科学研究院 水稻抗逆相关基因－海藻糖－6－磷酸磷酸化酶基因及其应用
US7314758B2 (en) 2005-01-07 2008-01-01 E.I. Du Pont De Nemours And Company Nucleotide sequences encoding RAMOSA3 and sister of RAMOSA3 and methods of use for same
ES2363980T3 (es) 2005-09-06 2011-08-22 Stichting Dienst Landbouwkundig Onderzoek Uso de una secuencia de ácido nucleico para la generación de plantas transgénicas que tienen tolerancia a la sequía mejorada.
WO2008124526A1 (en) * 2007-04-03 2008-10-16 Saphire Andrew C S Methods for transforming eukaryotic algae
EP2171085A4 (en) 2007-06-01 2010-07-14 Sapphire Energy SCREENING OF GENETICALLY MODIFIED PHOTOSYNTHETIC ORGANISMS WITH HIGH THROUGHPUT
WO2009017821A1 (en) * 2007-08-01 2009-02-05 Bioglow Inc. Bioluminescent plants comprising bacterial lux operon and methods of making same
AU2008299020A1 (en) * 2007-09-11 2009-03-19 Sapphire Energy, Inc. Molecule production by photosynthetic organisms
CA2698289A1 (en) * 2007-09-11 2009-03-19 Sapphire Energy, Inc. Methods of producing organic products with photosynthetic organisms and products and compositions thereof
EP2255003B1 (en) 2008-02-15 2015-01-28 Ceres Inc. Drought and heat tolerance in plants
CN101289514B (zh) * 2008-06-13 2011-01-19 北京北方杰士生物科技有限责任公司 一种培育耐逆植物的方法及其专用dna片段
US9175300B2 (en) 2008-11-27 2015-11-03 Vanga Siva Reddy Method of expressing foreign protein in plastids
KR20120126061A (ko) * 2009-10-22 2012-11-20 재단법인 작물유전체기능연구사업단 향상된 수확량 관련 형질을 갖는 식물 및 이의 제조 방법
EP2756072A4 (en) * 2011-09-13 2015-05-06 Stoller Ets PROCESS FOR INCREASING THE ERNOTE BY USING TREHALOSE
WO2013067259A2 (en) 2011-11-03 2013-05-10 Syngenta Participations Ag Regulatory nucleic acids and methods of use
GB201609969D0 (en) 2016-06-07 2016-07-20 Univ Cape Town Drought resistance multigene construct
CN106801102B (zh) * 2017-02-23 2020-11-20 浙江省检验检疫科学技术研究院 苋属的实时荧光pcr鉴定方法
EP3619300B1 (en) * 2017-05-04 2023-11-08 Cargill, Incorporated Genetically modified trehalase-expressing yeasts and fermentation processes using such genetically modified yeasts
US11242536B2 (en) * 2017-09-26 2022-02-08 Rutgers, The State University Of New Jersey Quality protein maize based on increased sulfur reduction in leaf cells
CN108624598A (zh) * 2018-06-06 2018-10-09 福建农林大学 Tppf基因在调控植物海藻糖合成和提高植物抗旱性中的应用
CN108728457B (zh) * 2018-06-22 2021-09-17 山东省药学科学院 一种海藻糖合成酶基因优化序列及其应用
CN121046427A (zh) * 2024-05-31 2025-12-02 中国科学院遗传与发育生物学研究所 一种提高植物愈伤组织诱导、转化及再生效率的方法
CN119744875A (zh) * 2024-12-26 2025-04-04 西华师范大学 苯并噻二唑在增强半夏光合系统稳定性和提高半夏耐旱性中的应用

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US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5422254A (en) 1992-02-14 1995-06-06 Oy Alko Ab Method to increase the trehalose content of organisms by transforming them with the structural genes for the short and long chains of yeast trehalose synthase
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US5792921A (en) * 1992-02-14 1998-08-11 Londesborough; John Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase
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MX205414B (es) * 1996-05-08 2001-12-07 Univ Mexico Nacional Autonoma Metodo para incrementar de trehalosa de los organismos por medio de su transformacion con el adnc de la trehalosa-6-fosfato sintasa/fosfatasa de selaginella lepidophylla.
EP0868426A4 (en) * 1996-07-23 2002-04-17 Syngenta Participations Ag CHEMICALLY INDUCABLE ARABIDOPSIS PR-1 PROMOTER
ZA989886B (en) * 1997-10-30 1999-04-30 Mogen Int Nuclear male sterile plants method of producing same and methods to restore fertility

Also Published As

Publication number Publication date
HUP0102598A3 (en) 2003-03-28
WO1999046370A2 (en) 1999-09-16
AU3329599A (en) 1999-09-27
CN1292824A (zh) 2001-04-25
AU748604B2 (en) 2002-06-06
IN2000CN00368A (https=) 2010-03-19
US20030009784A1 (en) 2003-01-09
JP2002505875A (ja) 2002-02-26
IL138191A0 (en) 2001-10-31
US6686516B2 (en) 2004-02-03
KR20010082520A (ko) 2001-08-30
EA200000926A1 (ru) 2001-06-25
PL343460A1 (en) 2001-08-13
WO1999046370A3 (en) 1999-11-18
BR9909258A (pt) 2000-11-28
CA2320896A1 (en) 1999-09-16
EP1064365A2 (en) 2001-01-03
US20040078848A1 (en) 2004-04-22
US7030294B2 (en) 2006-04-18
HUP0102598A2 (hu) 2001-11-28

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