TH9146C3 - Acid production process 5- aminolivulinic from photosynthetic bacteria - Google Patents

Abstract

DC60 (21/08/57) 1. Acid production process. 5- aminolivulinic From photosynthetic bacteria, the steps are as follows: a. By transferring the photosynthetic bacteria preserved in the GM or GS or Ormerod medium to cultivate the same formula in the culture vessel by adjusting the pH of the culture medium to 6.5- 7.0 with 10% NaOH before the sterile culture medium was contaminated by heating at 121 ° C for 15 minutes. B. By taking photosynthetic bacteria (Rhodopseudomonas palustris KG31 into the food to be cultured. Incubate the culture vessel at a temperature range of 35-40 ° C with one of the optional aeration methods: placing the culture vessel on a shaker at 200 rpm or aeration to provide a Dissolved oxygen equal to 5% in food is to create aeration and a mixture of nutrients to allow photosynthetic bacteria to grow. Until photosynthetic bacteria grow into Center distance locked to constant stage c. Production of 5-aminolivulinic acid The seedlings prepared from item b were transplanted into the GM food by using 5-10% of the seedling culture. Continuous aerator By controlling 5% dissolved oxygen and pH control to be in 6.5-7.0 with 10% NaOH and 20% H3PO4 until the cells grew to the middle stage, locked and filled with nitrogen gas. Or argon gas Or 2% carbon dioxide to dissolve 1-3% oxygen and control pH in this phase to equal 6.0–6.5 with 10% NaOH and 20% H3PO4 d. Into a constant distance 1.Acid production method 5- aminolivulinic From photosynthetic bacteria, the steps are as follows: a. By transferring the photosynthetic bacteria preserved in GM or GS or Ormerod medium to cultured in the same formula in the culture container, the pH of the culture medium was adjusted to the range of 6.5. -7.0 with 10% NaOH, prior to the sterilization of the cultured medium at 121 / C for 15 minutes. B. By taking photosynthetic bacteria (Rhodopseudomonas palustris KG31 into the food to be cultured. Incubate the culture vessel at a temperature range of 35-40 / C with one of the optional aeration methods, ie, place the culture vessel on a shaker. The speed range 200-250 per minute or aeration to make the oxygen. Dissolves in food equal to 5% to cause aeration and mixing of nutrients. For photosynthetic bacteria to grow Until photosynthetic bacteria C. Production of 5-aminolivulinic acid. The seedlings prepared from item b were transplanted into the GM food by using 5-10% of the seedling cultures. In the condition of continuous air filling By controlling dissolved oxygen in the range of 6.5-7.0 with 10% NaOH and 20% H3PO4, the cells grew to the middle phase, locked down and reduced the pH of the food. Cultures are 6.0-6.5 10% NaOH and 20% H3PO4 d. Discontinue cultures when cell growth reaches steady state. Edited (21 Aug 2014) 1. Acid production process 5- aminolivulinic From photosynthetic bacteria, the steps are as follows: a. By transferring the photosynthetic bacteria preserved in the GM or G5 or Ormerod formulated medium to cultivate the same formula in the culture vessel by adjusting the pH of the culture medium to 6.5- 7.0 with 10% NaOH, pre-contaminated with sterile culture medium at 121 ° C for 15 minutes. B. By taking photosynthetic bacteria Rhodopseudomonas palustris KG31 into the food that needs to be cultured. Incubate the culture container at 35-40 ° C with one of the optional aeration methods, such as placing the culture container on a shaker at 200 rpm or aeration for oxygenation. Soluble in food equal to 5% in order to cause aeration and mixing of nutrients to allow photosynthetic bacteria to grow. Until photosynthetic bacteria grow into Center distance locked to constant stage C. Production of 5-aminolivulinic acid The seedlings prepared from item b were transplanted into the GM food by using 5-10% of the seedling culture. Continuous aerator By controlling the dissolved oxygen 5% and pH to be in 6.5-7.0 with 10% NaOH and 20% H3PO4 until the cells grew to the middle stage, locked and filled with nitrogen gas. Or argon gas Or 2% carbon dioxide to dissolve 1-3% oxygen and control pH in this phase to equal 6.0–6.5 with 10% NaOH and 20% H3PO4 d. Into a constant distance

Claims (1)

1. Acid production process 5- Aminolivulinic from photosynthetic bacteria according to claim 1, where C-stage cultures are continuously cultured photosynthetic bacteria in the aeration state by controlling 5% dissolved oxygen and The pH control was in the range of 6.5-7.0 with 10% NaOH and 20% H3PO4 until the cell grew to the middle stage, locked and filled with nitrogen gas or aron gas. Or carbon dioxide at the rate of 2% to provide oxygen The solubility was in the 1-3% range and the pH was controlled in this phase to equal 6.0-6.5 with 10% NaOH 20%. H3PO4 revised (21 Aug 2014). 1. Acid production process 5- aminolivulinic From photosynthetic bacteria, the steps are as follows: a. By taking photosynthetic bacteria Rhodopseudomonas palustris KG31 which was stored in GM or G5 or Ormerod medium culture medium in The same food in the culture container. By adjusting the pH of food It was then cultured to be in 6.5-7.0 with 10% NaOH before sterilization. Contaminated with heat at temperature 121 degrees C for 15 minutes. B. By taking photosynthetic bacteria Rhodopseudomonas palustris KG31 into either GM or G5 or Ormerod formula. Incubate the culture container at 35-40 ° C with either aeration method. By placing the culture container on a shaker at a speed of 200 rpm or aeration to make dissolved oxygen equal to 5% in order to create aeration. And mixtures of nutrients For photosynthetic bacteria to grow It was cultured for 36-50 hours or until the photosynthetic bacteria had grown into the middle, locked to constant phase. C. Production of 5-aminolivulinic acid. The seedlings prepared from item b were transplanted into GM food by using 5-10% (volume by volume) of the germinated bacteria culture. Photosynthesis in a continuous aerated condition By controlling the dissolved oxygen 5% and pH to be in 6.5-7.0 with 10% NaOH and 20% H3PO4 until the cells grow to the middle lock phase. And add nitrogen gas Or argon gas or carbon dioxide at a rate of 2% to dissolve 1-3% of oxygen and control the The pH in this phase was equal to 6.0-6.5 with 10% NaOH and 20% H3PO4 d. Stop culturing when the cell growth has reached steady state.