TH9147C3 - Acid production process 5- aminolivulinic from photosynthetic bacteria - Google Patents

Abstract

DC60 (21/08/57) 1. Acid production process. 5- aminolivulinic From photosynthetic bacteria, the steps are as follows: a. By transferring the photosynthetic bacteria preserved in the GM or G5 or Ormerod formulated medium to cultivate the same formula in the culture vessel by adjusting the pH of the culture medium to 6.5- 7.0 with 10% NaOH before the sterile culture medium was contaminated by heating at 121 ° C for 15 minutes. B. By taking photosynthetic bacteria Rhodopseudomonas palustris KG31 into the food that needs to be cultured. Incubate the culture container at 35-40 ° C with one of the optional aeration methods: place the culture vessel on a shaker at 200 rpm or aeration to make oxygen. Soluble in food equal to 5% to cause aeration and mixing of nutrients to allow photosynthetic bacteria to grow. Until photosynthetic bacteria grow into Center distance locked to constant stage C. Production of 5-aminolivulinic acid The seedlings prepared from item b were transplanted into the GM food by using 5-10% of the seedling culture. Continuous aeration conditions By controlling dissolved oxygen 1-3% and controlling The pH of the cultured medium was in 6.5-7.0 with 10% NaOH and 20% H3PO4 until the cells reached the intermediate phase, locked down, and then reduced the pH of the culture medium to 6.0-6.5 with 10% NaOH and 20% H3PO4. d. Stop culturing when cell growth reaches steady state. Research reports confirm that the acid. 5- Aminolivulinic substances that promote plant growth, so this invention uses photosynthetic bacteria to produce it. The results of the invention clearly indicate that In aquaculture conditions with no light Can increase acid yield 5- aminolivulinic by bacteria Photosynthesis was achieved by controlling dissolved oxygen levels in combination with pH control. Edited (21 Aug 2014) 1. Acid production process 5- aminolivulinic From photosynthetic bacteria, the steps are as follows: a. By transferring the photosynthetic bacteria preserved in the GM or G5 or Ormerod formulated medium to cultivate the same formula in the culture vessel by adjusting the pH of the culture medium to 6.5- 7.0 with 10% NaOH, pre-contaminated with sterile culture medium at 121 ° C for 15 minutes. B. By taking photosynthetic bacteria Rhodopseudomonas palustris KG31 into the feed to be cultured formula. Incubate the culture container at 35-40 ° C with one of the optional aeration methods, such as placing the culture container on a shaker at 200 rpm or aeration for oxygenation. Soluble in food equal to 5% in order to cause aeration and mixing of nutrients to allow photosynthetic bacteria to grow. Until photosynthetic bacteria grow into Center distance locked to constant stage C. Production of 5-aminolivulinic acid The seedlings prepared from item b were transplanted into the GM food by using 5-10% of the seedling culture. Continuous aeration conditions By controlling dissolved oxygen 1-3% and controlling D. Acidity of the cultured medium is 6.5-7.0 with 10% NaOH and 20% H3PO4 until the cells have grown to the intermediate stage. Reduce the pH of the culture medium to 6.0-6.5 with 10% NaOH and 20% H3PO4. D. Stop the culture. When the growth of cells reaches a constant phase

Claims (1)

1. Process for producing acid substances 5- aminolivulinic from photosynthetic bacteria By the above method It consists of controlling the concentration of dissolved oxygen. And control of PH during the culture of photosynthetic bacteria. 2. The process of producing acid substances. 5- aminolivulinic from photosynthetic bacteria By the method mentioned in item 1 of the claim It consists of controlling the concentration of dissolved oxygen to be in the range of 5%. 3. Acids production process. 5- aminolivulinic from photosynthetic bacteria By the method mentioned in item 1 and 2 of the claim It consists of controlling the dissolved oxygen concentration in the 5% range and the pH control in the range 6.5-7.0. 4. Acids production process. 5- aminolivulinic from photosynthetic bacteria By the method mentioned in item 1, 2 and item 3 of the claim It consists of controlling the dissolved oxygen concentration in the range of 1-3%. 5. Acid production process. 5- aminolivulinic from photosynthetic bacteria By the process mentioned in items 1, 2, 3 and no.4 of the claim Consists of control of dissolved oxygen concentration in the 1-3% range and control of pH in the range 6.0-6.5 revised (21 Aug 2014). 1. Acid production process 5- aminolivulinic From photosynthetic bacteria, the steps are as follows: a. By taking photosynthetic bacteria Rhodopseudomonas palustris KG31 which was stored in GM or G5 or Ormerod medium culture medium in The same food in the culture container. By adjusting the pH of food It was then cultured to be in 6.5-7.0 with 10% NaOH before sterilization. Contaminated with heat at temperature 121 degrees C for 15 minutes. B. By taking photosynthetic bacteria Rhodopseudomonas palustris KG31 into either GM or G5 or Ormerod formula. Incubate the culture container at 35-40 ° C with either aeration method. By placing the culture container on a shaker at a speed of 200 rpm or aeration to make dissolved oxygen equal to 5% in order to create aeration. And mixtures of nutrients For photosynthetic bacteria to grow It was cultured for 36-50 hours or until the photosynthetic bacteria had grown into the middle, locked to constant phase. C. Production of 5-aminolivulinic acid. The seedlings prepared from item b were transplanted into the GM feed by using 5-10% (volume by volume) seedling content to cultivate photosynthetic bacteria in a continuous aerated condition. By controlling 5% dissolved oxygen and controlling pH during culture to 6.5-7.0 with 10% NaOH and 20% H3PO4 until the cells reached the middle lock phase. The culture medium was 6.0-6.5 with 10% NaOH and 20% H3PO4 d. Discontinued when the cell growth reached steady state.