TH9145C3 - Acid production process 5- aminolivulinic from photosynthetic bacteria - Google Patents

Abstract

DC60 (21/08/57) Acid processing method 5- aminolivulinic From photosynthetic bacteria, the steps are as follows: a. By transferring the photosynthetic bacteria preserved in the GM or GS or Ormerod medium to cultivate the same formula in the culture container, the pH of the culture medium was adjusted to 6.5-. 7.0 with 10% NaOH, pre-contaminated with sterile culture medium at 121 ° C for 15 minutes. B. By taking photosynthetic bacteria (Rhodopseudomonas palustris KG31 into the food to be cultured. Incubate the culture container at 35-40 ° C with one of the optional aeration methods: placing the culture vessel on a shaker at a speed of 200 rpm or aeration to oxygenate. Soluble in food equal to 5% in order to cause aeration and mixing of nutrients to allow photosynthetic bacteria to grow. Until photosynthetic bacteria grow into Center distance locked to constant stage C. Production of 5-aminolivulinic acid The seedlings prepared from item b were transplanted into the GM food by using 5-10% of the seedlings cultured with photosynthetic bacteria. Continuous aerator By controlling 5% dissolved oxygen and pH control to be in 6.5-7.0 with 10% NaOH and 20% H3PO4 until the cells reached the middle lock phase. And add nitrogen gas Or argon gas Or carbon dioxide at a rate of 2% to dissolve oxygen in 1-3% and pH control to be in 6.0-6.5 with 10% NaOH and 20% H3PO4 with addition of levulinic acid. And propionic acid at the amount of 10 mmolar and 4 mmolar, respectively. D. Inactivation was stopped when the cell growth reached steady state. Acid production process 5-aminolivulinic from photosynthetic bacteria, the steps are as follows: a. By transferring the photosynthetic bacteria preserved in the GM or GS or Ormerod medium to cultured in the same formula in the culture vessel, the pH of the cultured medium was adjusted to the range of 6.5. -7.0 with 10% NaOH, prior to the sterile culture of the culture medium at 121 / C for 15 minutes. B. By taking photosynthetic bacteria (Rhodopseudomonas palustris KG31 into the food to be cultured. Incubate the culture vessel at a temperature range of 35-40 / C with one of the optional aeration methods, ie, place the culture vessel on a shaker. The speed range 200-250 per minute or aeration to make oxygen. Dissolve in food equal to 5% to create aeration and mixing of nutrients. For photosynthetic bacteria to grow Until photosynthetic bacteria C. Production of 5-aminolivulinic acid. The seedlings prepared from item b were transplanted into the GM food by using 5-10% of the seedling to cultivate photosynthetic bacteria. In the condition of continuous air filling By controlling dissolved oxygen in the range of 6.5-7.0 with 10% NaOH and 20% H3PO4, the cell grew to the middle phase, locked and reduced the pH of the food. Culture is 6.0-6.5 10% NaOH and 20% H3PO4 d. Inactivation is stopped when cell growth reaches steady state. Edited (21 Aug 2014) 1. Acid production process 5- aminolivulinic From photosynthetic bacteria, the steps are as follows: a. By transferring the photosynthetic bacteria preserved in the GM or G5 or Ormerod medium to cultivate the same formula in the culture container, the pH of the culture medium was adjusted to 6.5-. 7.0 with 10% NaOH, pre-contaminated with sterile culture medium at 121 ° C for 15 minutes. B. By taking photosynthetic bacteria Rhodopseudomonas palustris KG31 into the food that needs to be cultured. Incubate the culture container at 35-40 ° C with one of the optional aeration methods: placing the culture vessel on a shaker at a speed of 200 rpm or aeration to oxygenate. Soluble in food equal to 5% in order to cause aeration and mixing of nutrients to allow photosynthetic bacteria to grow. Until photosynthetic bacteria grow into Center distance locked to constant stage C. Production of 5-aminolivulinic acid The seedlings prepared from item b were transplanted into the GM food by using 5-10% of the seedlings cultured with photosynthetic bacteria. Continuous aerator By controlling the dissolved oxygen 5% and pH to be in 6.5-7.0 with 10% NaOH and 20% H3PO4 until the cells reached the middle lock phase. And add nitrogen gas Or argon gas Or carbon dioxide at a rate of 2% to dissolve oxygen in 1-3% and pH control to be in 6.0-6.5 with 10% NaOH and 20% H3PO4 with addition of levulinic acid. And propionic acid at the amount of 10 mmolar and 4 mmolar, respectively. D. Inactivation was stopped when the cell growth reached steady state.

Claims (1)

1. Acid production process 5- Aminolivulinic from photosynthetic bacteria according to claim 1, where C-stage culture was performed by culturing photosynthetic bacteria in a continuous aeration state with 5% dissolved oxygen control The pH control was in the range 6.5-7 with 10% NaOH and 20% H3PO4 until the cells reached the middle lock phase and filled with nitrogen gas or argon gas. Or carbon dioxide at the rate of 2% to provide oxygen Solubility in the 1-3% range and pH control to be in the range 6.0-6.5 with 10% NaOH and 20% H3PO4 with addition of 10mL of levulinic and propionic acid. Molar and 4 mmolar respectively. Edit (21 Aug 2014) 1. Acid production process 5- aminolivulinic From photosynthetic bacteria, the steps are as follows: a. By taking photosynthetic bacteria Rhodopseudomonas palustris KG31 which was stored in GM or G5 or Ormerod medium culture medium in The same food in a culture container By adjusting the pH of food Such cultures were then cultured to 6.5-7.0 with 10% NaOH prior to sterilization. Contaminated with heat at temperature 121 degrees C for 15 minutes. B. By taking photosynthetic bacteria Rhodopseudomonas palustris KG31 into either GM or G5 or Ormerod formula. Incubate the culture container at 35-40 ° C with either aeration method. By placing the culture container on a shaker at a speed of 200 rpm or aeration to make dissolved oxygen equal to 5% in order to create aeration. And mixtures of nutrients For photosynthetic bacteria to grow It was then cultured for 36-50 hours or until the photosynthetic bacteria had grown into the middle, locked to constant phase. C. Production of 5-aminolivulinic acid. The seedlings prepared from item b were transplanted into the GM food by using 5-10% (volume by volume) of the germinating bacteria. Photosynthesis in a continuous aerated condition By controlling the dissolved oxygen 5% and pH to be in 6.5-7.0 with 10% NaOH and 20% H3PO4 until the cells grow to the middle lock phase. And add nitrogen gas Or argon gas or carbon dioxide at a rate of 2% to dissolve 1-3% of oxygen and control the The pH was 6.0-6.5 with 10% NaOH and 20% H3PO4, together with the addition of 10 mmolaric acid and propionic acid, and 4 mmolar d . Stop culturing when cell growth reaches a constant phase.