TH22964Y - The process of producing cordycepin from the mycelium of Cordyceps sinensis mushrooms. - Google Patents

Abstract

------August 26, 2020------(OCR) A method for producing cordycepin from Cordyceps sinensis mycelium using water, potato extract, or PDB liquid medium, and sugar as the main raw materials, mixed with peptone and yeast extract in liquid form. No animal protein, such as worms or pupae, is used in the culture medium, unlike currently used Cordyceps sinensis culture formulas. This liquid culture medium reduces the cultivation time by 30 days, allowing for faster harvesting. It also reduces electricity costs by 36%. Furthermore, it yields Cordyceps sinensis mycelium with very high levels of cordycepin and adenosine (up to 15 mg/g dry weight), which can be processed into dietary supplements, herbal teas for health, or as components of traditional Chinese medicine. ------------ Page 1 of 1 Summary of the Invention: Production of Cordycepin from Cordyceps sinensis Mycelium This culture medium consists primarily of potato extract or PDB liquid medium and sugar, mixed with peptone and yeast extract. Unlike commonly used Cordyceps sinensis culture media, it does not use animal proteins such as worms or pupae. This liquid medium reduces the cultivation time by 30 days, allowing for faster harvesting and reducing electricity costs by 36%. Furthermore, it yields high levels of Cordyceps sinensis mycelium containing cordycepin and adenosine (up to 15 mg/g dry weight), which can be processed into dietary supplements, health-boosting herbal teas, or used as ingredients in traditional Chinese medicine.

Claims (4)

------26/08/2563------(OCR) 1. The process for producing cordycepin from Cordyceps sinensis mycelium consists of: a. Mixing the components, namely carbohydrates (68-75% by weight) and protein (25-30% by weight), with 1 liter of clean water added and stirred thoroughly. b. Dividing the mixture from step a into cultivation containers, filling them to 25% of their capacity. Closing the containers by slightly loosening the screw caps or using cotton plugs. c. Sterilizing the prepared culture medium from step b by steam sterilization at 121°C, 15 psi pressure for 15 minutes, and then cooling to 25°C. 2. The process for producing cordycepin from Cordyceps sinensis mycelium, as per claim 1, involves the following steps for cultivating Cordyceps sinensis: a. Adding 10% of the initial Cordyceps sinensis inoculum to the culture medium prepared in step 1. Closing the container tightly and cultivating it in an air-conditioned room. or a refrigerator with a temperature of 20-22 degrees Celsius, with the light on 24 hours a day for 30 days. b. After the cultivation of Cordyceps sinensis for the 30-day period, the Cordyceps sinensis mycelium can be harvested and processed into dried mycelium by drying at a temperature of 55-60 degrees Celsius continuously for at least 6 hours. 3. The process for producing cordycepin from Cordyceps sinensis mycelium according to claim 1, where carbohydrates can be selected from potatoes, petibial liquid medium, glucose, either individually or in combination. 4. The process for producing cordycepin from Cordyceps sinensis mycelium according to claim 1, where protein can be selected from peptone and yeast extract, combined. ------------ Page 1 of 1 page Claims 1. Cordycepin from the mycelium of Cordyceps sinensis contains 68-75 percent carbohydrates by weight and 25-30 percent protein by weight. 2. Corydycepin from Cordyceps sinensis mycelium - as per claim 1, where carbohydrates are selectable from potatoes, PDB liquid medium, and glucose, either individually or in combination. 3. The formula for cultivating Cordyceps sinensis mushrooms, as per claim 1, in which selectable proteins from peptone and yeast extract are combined. 4. The formula for cultivating Cordyceps sinensis mushrooms, as per claim 1, involves the following production process: a. Combine the components: carbohydrates (68-75% by weight) and protein (25-30% by weight). Add 1 liter of clean water and stir thoroughly. b. Divide the mixture from step a into cultivation containers, filling them to 25% of their capacity. Close the lids by slightly loosening the screw caps or using cotton plugs. c. Sterilize the prepared culture medium by steam sterilization at 121°C, 15 psi, for 15 minutes. Then cool to 25°C. d. Pour the starter culture into the culture medium from step c. Seal the containers tightly and cultivate the mushrooms in an air-conditioned room or refrigerator at 20-22°C for 30 days. e. After 30 days of cultivation, the Cordyceps sinensis mycelium can be harvested and processed into dried mycelium. This is achieved by baking at a temperature of 55-60 degrees Celsius continuously for at least 6 hours.