KR101517845B1 - Method of producing a fertilizer capable of generating folic acid in apples using at least two fementedmatured vegetables based on beans - Google Patents

Abstract

The present invention is based on a technology for producing a fermented and aged fertilizer wherein the technology transfers a bean′s capacity of producing folic acid to fruits (apples) by using a fertilizer, which is obtained by fermenting and aging beans, thereby enabling a production of folic acid even in apples. A concentrate of a fermented liquid, which is obtained by fermenting beans, is fermented and produced into a seed culture so as to act as a seed culture when two or more vegetables are mixed and fermented, and prevents any production process problem even in the mixing of the vegetables. The present invention has been produced by mixing and fermenting beans and spinach, and more specifically, is a fertilizer obtained by complexly fermenting and aging beans, roots of spinach, and leaves and stems of spinach. A seed culture of the fermentation and aging has been secured and a process technology for mass production has been established. In addition, a comparative experiment has been carried out against apples originated from various regions through a control group and a treatment group by applying a fertilizer produced by the technology of the present invention. It has been confirmed in the result of the experiment that apples containing the largest amount of folic acid in a natural state, which is more than 200 ug per 100 g, have been produced.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a fertilizer producing method, and more particularly, to a method for producing foliar fertilizer,

The present invention relates to a method for producing a fertilizer using a fermented bean and a spinach. By foliar application of this fermentation liquid to the fruit tree, the fruit can be a fruit containing folic acid.

Folate (folate) is a water-soluble vitamin belonging to the vitamin B group. Folate is widely distributed in green plants and its name comes from the Latin 'folium', which means 'leaf'. Folate was formerly known as 'folic acid' or 'folacin', but today 'folate' is the common name for this vitamin.

Folic acid is essential for the synthesis of amino acids and nucleic acids (nucleic acids). Especially, it is important for cell division and growth because it is involved as a coenzyme of enzymes involved in DNA replication, which is a nucleic acid that makes genes. Folic acid is a vitamin that binds to vitamin B12 and focuses on growth and red blood cell production, promoting the secretion of the neurotransmitter, noradrenaline, in the brain.

Folic acid, taken as food, is converted to monoglutamate by the folyl polyglutamate hydrolase in the small intestine and then absorbed. The absorption rate of folic acid is 90% for the single glutamic acid form and 50 to 90% for the polyglutamic acid form. Protein-bound folate is absorbed in the small intestine and then converted to 5-methyl-THF in the liver and released into the blood. Folic acid is known to contribute to lowering the risk of hypertension by relaxing the blood vessels so that the blood flows well.

Folic acid is widely distributed in food. Folic acid was first isolated from spinach, and folic acid-rich foods include liver, yeast, soybeans, vegetables, and fruits.

The folate that can be consumed through meals is 250-500 g per day, so 500 g of folic acid supplement can be taken 1,000 g a day. According to Korean nutrition guidelines, the recommended daily intake of folic acid for adults is 400 μDDFE.

50 to 90% of the folic acid contained in the food is lost during cooking and processing. Particularly, it is almost destroyed after over processing.

A new method for consuming such folic acid has been studied, and the inventor of the present invention intends to provide a method of producing a fruit containing folic acid by using a fertilizer.

Korean Patent Publication No. KR 10-0974426 B1 Japanese Patent Application Laid-Open No. 1999-106276 A Korean Patent Publication No. 10-2006-0059631 A

The present invention provides a method for making a fertilizer for foliar fertilization to produce fruit containing folic acid.

The fertilizer containing the amino acid capable of producing folic acid according to the present invention can be a fruit containing folic acid by foliar application to the fruit tree.

A method of manufacturing a fertilizer using a fermented bean and a spinach according to an embodiment of the present invention includes: boiling and boiling beans in water; Adding sugar to boiled beans and soybeans; Placing the beans in a closed container and fermenting them; Periodically shaking the enclosed container to mix beans and injecting air; Preparing a fermentation-aged soybean fermentation broth obtained through a step of opening the sealed container and bringing it into contact with air and aging;

Preparing spinach roots and boiling the spinach roots with water; Boiling the spinach with a pressurizer; Adding soybean fermented soybean concentrate and sugar and mixing; Placing the spinach in a closed container and fermenting it; Periodically shaking the sealed container to mix the spinach and injecting air; Preparing a fermented fermented spinach root fermentation broth obtained through a step of opening the sealed container and bringing it into contact with air and aging;

Preparing spinach leaves and stems and pulverizing the spinach leaves and stems; Milling the pulverized spinach leaves and stem; Adding soybean fermented soybean concentrate and sugar and mixing; Placing the spinach in a closed container and fermenting it; Periodically shaking the sealed container to mix the spinach and injecting air; Preparing a fermented aged spinach leaf and a stem fermentation liquid obtained through a step of opening the sealed container and bringing it into contact with air and aging;

Preparing a fermented soybean fermentation broth and fermentation-aged spinach root fermentation broth obtained by preparing the fermentation-aged soybean fermentation broth; And mixing the spinach leaves and the stem fermentation broth obtained by the step of preparing the fermentation-aged spinach leaves and stem fermentation broth, followed by aging by opening with air.

In this case, the soybean fermentation broth, the spinach root fermentation broth, the spinach leaf and the stem fermentation broth are mixed with each other and then opened with air to obtain a fermentation broth. The fermentation broth is sterilized using sodium acid sulfite and then sealed May further include a step of storing.

Meanwhile, the soybean fermentation broth concentrate to be mixed at the step of preparing the spinach root fermentation broth and the step of preparing the spinach leaf and stem fermentation broth may be prepared by adding the soybean into the closed container and preparing fermented soybean fermentation broth Is the soybean fermentation broth extracted after the step.

The step of fermenting the soybeans into a closed container and fermenting the spinach in a closed container is carried out for two weeks in a dark room at a temperature of 30 DEG C or less and at a pressure of 1.1 atm with shielding the inflow of air.

The content of the sugar is preferably 50% by weight or more in the whole mixture.

According to the present invention, when the soybean and spinach is fermented and fermented and fermented, the fruit of the fruit tree is made into a fruit containing folic acid when the fruit is foliar applied to the fruit tree.

It is possible to take folic acid by using this fruit and it is possible to eat very effective folic acid.

This technology is based on fermentation-aged fertilizer production technology that transfers folate production ability of soybean to fruit tree (apple) through fertilizer produced by fermentation of soybean to produce folate in apple. Fermenting the concentrate of the fermentation broth through the fermentation of soybeans to ferment the present fertilizer to make the two or more kinds of plants perform a seeding function during the fermentation of the mixture and mixing them to prevent a problem in a separate manufacturing process .

FIG. 1 is a flow chart of a method of manufacturing a fertilizer using a fermented bean and spinach according to an embodiment of the present invention.
FIG. 2 is a flowchart illustrating a step of preparing a fermentation-aged soybean fermentation broth according to an embodiment of the present invention.
FIG. 3 shows a flowchart of a step of preparing a fermented fermented spinach root fermentation broth according to an embodiment of the present invention.
FIG. 4 is a flowchart illustrating steps of preparing fermented aged spinach leaves and stem fermentation broths according to an embodiment of the present invention.
Various embodiments are now described with reference to the drawings, wherein like reference numerals are used throughout the drawings to refer to like elements. For purposes of explanation, various descriptions are set forth herein to provide an understanding of the present invention. It is evident, however, that such embodiments may be practiced without these specific details. In other instances, well-known structures and devices are shown in block diagram form in order to facilitate describing the embodiments.

The following description provides a simplified description of one or more embodiments in order to provide a basic understanding of embodiments of the invention. This section is not a comprehensive overview of all possible embodiments and is not intended to identify key elements or to cover the scope of all embodiments of all elements. Its sole purpose is to present the concept of one or more embodiments in a simplified form as a prelude to the more detailed description that is presented later.

The technology of the present invention is based on a fermentation-aged fertilizer production technology that transfers folate-producing ability of soybeans to fertilizer (apple) through fertilizer produced by fermentation of soybean to produce folic acid in apple. Fermenting the concentrate of the fermentation broth through the fermentation of soybeans to ferment the present fertilizer to make the two or more kinds of plants perform the seeding at the time of mixed fermentation and to prevent the problems in the separate manufacturing process from occurring even when they are mixed . At present, the technology of the present invention is produced by mixing fermented soybeans and spinach, and more specifically, three kinds of fermented fermented manure such as soybean, spinach root, spinach leaf and stem. These fermentation aged seeds were secured and process technology for mass production was established. In addition, a comparative experiment was carried out by treating the fertilizer produced by the technique of the present invention with a control of a variety of local apples. As a result, an apple having 200 g or more of folic acid per 100 g having the highest content in the natural state was produced .

FIG. 1 is a flow chart of a method of manufacturing a fertilizer using a fermented bean and spinach according to an embodiment of the present invention.

According to the flowchart of FIG. 1, the method for manufacturing a fertilizer using a fermented bean and spinach according to an embodiment of the present invention comprises: (S 110) preparing a fermented soybean fermentation broth; Preparing a fermented fermented spinach root fermentation solution (S 120); Preparing fermented aged spinach leaves and stem fermentation broth (S 130); (S 140) mixing and mixing the fermentation broth and opening it with air to mature; And a step (S 150) of sterilizing the aged solution using sodium acidic sodium sulfate, and sealing and storing the aged solution.

Steps S 110 to S 130 will be described in detail below, and then steps S 140 and S 140 will be described.

The step (S 110) of preparing the fermented aged soybean fermentation broth is shown in the flowchart of FIG. 2, and the respective steps are as follows.

The step of preparing the fermentation-aged soybean fermentation broth according to an embodiment of the present invention comprises: (a) a step (S 210) of boiling and boiling soybeans in water; Adding sugar to boiled beans and soybeans (S220); Placing the beans in a closed container and fermenting the beans (S230); Periodically swinging the sealed container to mix the beans and inject air (S 240); And a step (S 250) of opening the sealed container and bringing it into contact with air and aging.

There is no particular restriction on soybeans, and soybeans are known as folic acid-based foods, and the content of folic acid generally includes 105 μg per 100 g.

In step S 210, beans are prepared, and the prepared beans are boiled in water and then boiled. In this case, it is preferable to be called for about 24 hours or longer so that the beans can be sufficiently burned. This is because, if the time for which beans are called is short, the fermentation liquid may not contain enough amino acids to generate enough folic acid when fermenting the soybeans.

After soaking the beans in the water, the beans are boiled and boiled. In this case, it is preferable to boil until the size of the beans is larger than 30% of the original size. This part is also intended to sufficiently contain the amino acid capable of producing folic acid in the soybean during the fermentation liquid extraction by boiling the soybeans sufficiently.

In step S 220, sugar is added to the boiled water of beans and beans. In this case, the ratio is important, and the proportion of sugar is preferably at least about 50% by weight of the total weight%. If the amount of sugar is more than 50% by weight, the fermentation can be sufficiently performed, and the amino acid capable of producing folic acid is sufficiently contained in the fermentation liquid.

In step S 230, the mixture prepared in step S 220 is placed in a closed container and fermented. The closed container may be any container which can be sealed with the outside, and in this case, it is preferable that an air inlet is provided. Fermentation conditions are carried out at a temperature of 30 ° C or less and a pressure of 1.1 atmospheres under dark room conditions. The fermentation time is about 2 weeks. In order to maintain 1.1 atmospheric pressure in the dark room, it is preferable to use a pressure vessel. Gas is generated during fermentation by this pressure vessel, and the gas is automatically discharged to the outside while keeping it at a certain pressure. Prevents the introduction of covert bacteria into fermentation fixation.

Meanwhile, in the present specification, the dark room means a cool place where indirect light is present as a shadow place where a direct light source is blocked.

In this case, the fermentation broth fermented for two weeks can be extracted, and the extracted fermentation broth is used as a soybean fermentation broth concentrate in the process of obtaining the spinach root and the fermentation solution of the mouth and stem to be described later.

In step S 240, the sealed container is shaken periodically to mix the beans and inject air. The air inlet is formed through the air inlet formed in the above-described airtight container. Shaking and air injection for this mixing is done periodically, preferably twice a day for one month. After about 6 months, the process of adding air only once a month and adding air will be continued for about 6 months.

The concept of shaking the container in this specification includes all the possible operations for allowing the contents of the container inside and outside the container to be mixed with air evenly. When mass production is performed, the mixer is rotated using a motor so that the up- It is to be contacted and mixed.

In step S 250, the sealed container is opened and the inside contents are aged while being in contact with air, thereby finally obtaining a fermented soybean fermented for 12 months or longer.

The step (S 120) of preparing the fermented aged spinach root fermentation liquid is shown in Fig. 3, and the respective steps are as follows.

The step of preparing fermented fermented spinach root fermentation broth according to an embodiment of the present invention includes preparing a spinach root and boiling the spinach root with water (S 310); Boil the spinach with a pressurizer (S 320); A step (S 330) of mixing and mixing the soybean fermented soybean concentrate and sugar; Placing the spinach in a closed container and fermenting (S 340); Periodically shaking the sealed container to mix the spinach and inject air (S 350); And a step (S 360) of opening and sealing the sealed container in contact with air.

There is no particular restriction on spinach, and spinach is known as a source of folic acid. In the case of spinach, it contains 196.2 μg of folic acid per 100 g.

In step S 310, the roots of the spinach are cut off and washed thoroughly before boiling the spinach roots with water. In this case, the amount of water is preferably at least five times the weight of the root. The amount of water is such that the content of amino acids capable of producing folic acid contained in roots in the spinach can be sufficiently contained in the spinach root fermentation broth similarly to the above-described fermentation of soybean fermentation broth.

In step S 320, the cooked spinach is squeezed by a pressure gauge in step S 310. There is no particular restriction on the pressure gauge. Anything that can be juiced by pressing the spinach with a pressure gauge is possible.

In step S 330, soybean fermented soybean concentrate and sugar are added to the spinach juice and mixed.

As the concentrated soybean fermentation broth, a concentrate prepared by extracting the primary fermentation broth fermented at step S 230 in FIG. 2 and aging it at a temperature of 10 ° C or less is used. That is, soybean fermentation microorganisms are used for fermentation of spinach for fermentation, so that even when two or more kinds of plants such as soybean and spinach are mixed and fermented, two or more kinds of plants are mixed using the same kind of seed (step S 140 Beans and spinach fermentation broths). Therefore, it is important to extract soybean fermented soybean concentrate and add it as a seedling when fermenting the roots, leaves and stems of spinach. It is preferred that the soybean fermented soybean concentrate is less than about 1% by weight of the total weight.

On the other hand, it is preferable that the ratio of sugar is about 50% by weight or more of the total weight%, and if the amount of sugar is 50% by weight or more, sufficient fermentation can be achieved and amino acid capable of producing folic acid is sufficiently contained in the fermentation liquid extraction .

In step S 340, the mixture prepared in step S 330 is placed in a closed container and fermented. The closed container may be any container which can be sealed with the outside, and in this case, it is preferable that an air inlet is provided. Fermentation conditions are carried out at a temperature of 30 ° C or less and a pressure of 1.1 atmospheres under dark room conditions. The fermentation time is about 2 weeks.

In step S 350, the sealed container is shaken periodically to mix the internal mixture and inject air. The air inlet is formed through the air inlet formed in the above-described airtight container. Shaking and air injection for this mixing is done periodically, preferably twice a day for one month. After about 6 months, the process of adding air only once a month and adding air will be continued for about 6 months.

In step S 360, the closed container is opened and the inside contents are aged while being in contact with air. Finally, the fermented spinach root fermented for 12 months or longer is obtained.

The step (S 130) of preparing the fermented aged spinach leaves and the stem fermentation liquid is shown in FIG. 4, and the respective steps are as follows.

Preparing the fermented aged spinach leaves and stem fermentation broth according to an embodiment of the present invention includes preparing spinach leaves and stem and pulverizing the spinach leaves and stem (S 410); A step (S420) of squeezing the spinach leaves and stems with a pressurizer; Mixing and mixing the soybean fermented soybean concentrate and sugar (S 430); Placing the spinach in a closed container and fermenting (S 440); Periodically shaking the sealed container to mix the spinach and inject air (S 450); And a step (S 460) of opening and sealing the sealed container in contact with air.

There is no particular restriction on spinach, and spinach is known as a source of folic acid. In the case of spinach, it contains 196.2 μg of folic acid per 100 g.

In step S 410, the leaves and stems of the spinach are cut and sufficiently washed, and then the leaves and stem are pulverized to a size of 0.1 mm or less using a pulverizer. The pulverizer is not particularly limited, and any pulverizer capable of pulverizing to a size of 0.1 mm or less is usable.

The reason why the leaves and the stem are crushed to less than 0.1 mm is that crushing should be performed to such a size that when the fermentation liquid is obtained by the pressurization with a pressurizing device, sufficient amino acid capable of producing folic acid is sufficiently contained.

In step S 420, the spinach crushed in step S 410 is squeezed by a pressure gauge. There is no particular restriction on the pressure gauge. Anything that can be juiced by pressing the spinach with a pressure gauge is possible.

In step S 430, soybean fermented soybean concentrate and sugar are added to the spinach juice solution and mixed.

As the concentrated soybean fermentation broth, a concentrate prepared by extracting the primary fermentation broth fermented at step S 230 in FIG. 2 and aging it at a temperature of 10 ° C or less is used. That is, soybean fermentation microorganisms are used for fermentation of spinach for fermentation, so that even when two or more kinds of plants such as soybean and spinach are mixed and fermented, two or more kinds of plants are mixed using the same kind of seed (step S 140 Beans and spinach fermentation broths). Therefore, it is important to extract soybean fermented soybean concentrate and add it as a seedling when fermenting the roots, leaves and stems of spinach. It is preferred that the soybean fermented soybean concentrate is less than about 1% by weight of the total weight.

On the other hand, it is preferable that the ratio of sugar is about 50% by weight or more of the total weight%, and if the amount of sugar is 50% by weight or more, sufficient fermentation can be achieved and amino acid capable of producing folic acid is sufficiently contained in the fermentation liquid extraction .

In step S 440, the mixture prepared in step S 430 is placed in a closed container and fermented. The closed container may be any container which can be sealed with the outside, and in this case, it is preferable that an air inlet is provided. Fermentation conditions are carried out at a temperature of 30 ° C or less and a pressure of 1.1 atmospheres under dark room conditions. The fermentation time is about 2 weeks.

In step S 450, the sealed container is shaken periodically to mix the internal mixture, and air is injected. The air inlet is formed through the air inlet formed in the above-described airtight container. Shaking and air injection for this mixing is done periodically, preferably twice a day for one month. After about 6 months, the process of adding air only once a month and adding air will be continued for about 6 months.

In step S 460, the sealed container is opened, and the contents of the inside of the container are aged while being in contact with air. Finally, the fermented spinach root fermented for 12 months or more is obtained.

In step S 140, spinach root fermentation broth obtained by preparing soybean fermentation broth and fermented fermented spinach root fermentation broth obtained by preparing the fermentation-aged soybean fermentation broth respectively obtained in FIGS. 2, 3 and 4; And spinach leaves and stem fermentation broth obtained by fermenting aged spinach leaves and stem fermentation broth are mixed and mixed with each other, and opened with air to be aged.

In this case, the ideal ratio is 80% of soybean fermentation, 10% of spinach root fermentation, and 10% of spinach leaf and root fermentation. Aging is mixed in the same ratio as above, and it is matured with air and open for a month.

Step S 150 is a step for processing the aging solution obtained in step S 140, and is a step for facilitating storage. After sterilization of the aging solution with acidic sodium sulphate, it should be kept sealed. The obtained fertilizer can be stored for a long time with sufficient amino acid capable of producing folic acid. The acidic sodium sulfite is preferably mixed at about 2 g per kg. The specific use period of this acidic sodium sulfite is mixed with the fermented aged fertilizer 10 days before shipment.

Table 1 below is an ingredient composition table of the fertilizer produced by the method of the present invention. As shown in the table, it can be confirmed that the foliar fertilization of fruit trees contains enough amino acids to produce folic acid.

The following data were obtained from the following mixing ratios. The mixing ratio of soybean, water and sugar in the preparation stage of fermentation-aged soybean fermentation broth was 10 wt%, 35 wt% and 55 wt%, respectively, The mixture ratio of spinach root juice, soybean fermentation broth concentrate, and sugar in the step of preparing the spinach root fermentation broth was 40 wt%, 1 wt% and 59 wt%, respectively. In the preparation of fermented aged spinach leaves and stem fermentation broth The mixture ratio of spinach leaf and stem juice, soybean fermented soybean concentrate and sugar was 40 wt%, 1 wt% and 59 wt%, respectively. The content of sugar is preferably 50% by weight or more in the whole mixture as described above.

Test Results Aspartic Acid 56mg / 100g Serine 23mg / 100g Glutamic Acid 74mg / 100g Glycine 180mg / 100g Histidine Not detected Arginine Not detected Threonine 90mg / 100g Alanine 57mg / 100g Proline 33mg / 100g Cystine Not detected Tyrosine Not detected

Table 2 below shows the results of the test report obtained by analyzing the components of apples obtained from the tree after the application of the fertilizer according to the present invention to the apple trees.

As shown in the table below, it was confirmed that 141.97 μg of folic acid was contained per 100 g. As a result, it was confirmed that the fertilizer according to the present invention fully contains amino acids capable of generating folic acid.

Test Results Exterior A unique form of red apple Vitamin B1 (mg / 100g) 0.14 Folic acid (μg / 100g) 141.97 Niacin (mg / 100 g, NE) 0.02 Beta-carotene (μg / 100g) 43.37

The description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features presented herein.

Claims (5)

Boiling and boiling beans in water; Adding sugar to boiled beans and soybeans; Placing the beans in a closed container and fermenting them; Periodically shaking the enclosed container to mix beans and injecting air; Preparing a fermentation-aged soybean fermentation broth obtained through a step of opening the sealed container and bringing it into contact with air and aging;
Preparing spinach roots and boiling the spinach roots with water; Boiling the spinach with a pressurizer; Adding the soybean fermented soybean concentrate and sugar to the juiced spinach; Placing the mixture in a closed container and fermenting it; Periodically shaking the closed container to mix the mixture and inject air; Preparing a fermented fermented spinach root fermentation broth obtained through a step of opening the sealed container and bringing it into contact with air and aging;
Preparing spinach leaves and stems and pulverizing the spinach leaves and stems; Milling the pulverized spinach leaves and stem; Adding the soybean fermented soybean concentrate and sugar to the juiced spinach; Placing the mixture in a closed container and fermenting it; Periodically shaking the closed container to mix the mixture and inject air; Preparing a fermented aged spinach leaf and a stem fermentation liquid obtained through a step of opening the sealed container and bringing it into contact with air and aging;
Preparing a fermented soybean fermentation broth and fermentation-aged spinach root fermentation broth obtained by preparing the fermentation-aged soybean fermentation broth; And a step of mixing the spinach leaves and the stem fermentation broth obtained by preparing the fermentation-aged spinach leaves and stem fermentation broth, followed by aging by opening with air,
Fertilizer production method using soybean and spinach fermentation broth.
The method according to claim 1,
After mixing the soybean fermentation broth, the spinach root fermentation broth, and the spinach leaves with the stem fermentation broth, the broth is opened with air to be aged to obtain a fermentation broth. Thereafter, the fermentation broth is sterilized using sodium acid sulfite, As a result,
Fertilizer production method using soybean and spinach fermentation broth.
The method according to claim 1,
The soybean fermented soybean concentrate, which is mixed in the step of preparing the spinach root fermentation broth and the step of preparing the spinach leaves and stem fermentation broth,
Wherein the soybean fermentation broth is a soybean fermentation broth extracted after the step of fermenting the beans in a closed container in the step of preparing the fermentation-aged soybean fermentation broth.
Fertilizer production method using soybean and spinach fermentation broth.
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