TH19235Y - Manufacturing process of prototype probiotic products for pigs. - Google Patents

Abstract

------March 19, 2021------(OCR) This invention describes the development and improvement of selected probiotics with desirable properties suitable for pigs in Thailand. The effectiveness was enhanced through a two-layer encapsulation process, consisting of an inner layer of alginate and an outer layer of chitosan, using the spray-drying method. The resulting prototype probiotic product is a fine powder, convenient for storage, practical use in pig farms, and transportation, and effectively stimulates pig growth performance. ------------ ------September 19, 2019------(OCR) Page 1 of 1. Invention Summary: The selection of probiotics with desirable properties suitable for pigs in Thailand was further developed and improved through a two-layer encapsulation process, consisting of an inner layer of alginate and an outer layer of chitosan, using the spray-drying method. The resulting prototype probiotic product is a fine powder, convenient for storage, practical use in pig farms, and transportation, and effectively stimulates pig growth performance. And it can effectively stimulate the growth performance of pigs. ------------ ------19/09/2019------(OCR) Page 1 of 1 page Summary of the Invention The selection of probiotics with good and suitable properties for pigs in Thailand was further developed and improved to increase their efficiency through a double-layer encapsulation process. The encapsulation consists of an inner layer of alginate and an outer layer of chitosan, using the spray-drying method. This results in a prototype probiotic product in the form of a small powder, convenient for storage, practical use in pig farms, and transportation. It can effectively stimulate the growth performance of pigs. ------------ Page 1 of 1 page Summary of the Invention The selection of probiotics with good and suitable properties for pigs in Thailand was further developed and improved to increase their efficiency through a double-layer encapsulation process. The encapsulation consists of an inner layer of alginate and an outer layer of chitosan, using the spray-drying method. This results in a prototype probiotic product in the form of a small powder, convenient for storage, practical use in pig farms, and transportation. It can effectively stimulate the growth performance of pigs.

Claims (3)

------19/03/2564------(OCR) 1. The process for producing a prototype probiotic product for pigs is as follows: a. Preparation of the coating materials: The inner coating consists of 1.5% W/V alginate dissolved in water, and the outer coating consists of 0.5% W/V chitosan dissolved in 0.1% W/V acidic acid. b. Encapsulation of the probiotic: The inner coating is made of alginate, and a probiotic culture at a concentration of 10⁹ colonies per milliliter is then added. Mix with 1.5% alginate (weight/volume) dissolved in water from step a, in a ratio of 1 part to 5 parts. Take the mixture from step b and dry it using a spray dryer, setting the inlet temperature to 120-140 degrees Celsius. Take 1 gram of the powder from step c and encapsulate it with chitosan at a concentration of 0.5% (weight/volume). 1. A. A method for producing a prototype probiotic product for pigs, as described in claim a., by dissolving 100 ml of the mixture in 0.1% acetic acid by weight/volume. 2. B. C. D. E. F ... The process is as follows: 1. Preparation of the coating materials: a. The inner coating consists of 1.5% w/v alginate dissolved in water, and b. The outer coating material is 0.5% w/v chitosan dissolved in 0.1% w/v acetic acid. 2. Coating the probiotics: c. The inner coating is made of alginate, and a probiotic culture at a concentration of 10⁹ colonies per milliliter is introduced. 1. Mix with 1.5% alginate by weight/volume in a 1:5 ratio. 2. The mixture from step c is then finely ground and dried using a spray dryer with the inlet temperature set to 120-140°C, with the optimal temperature being 130°C. 3. Encapsulation with chitosan. 3. Encapsulation with chitosan. 4. Take 1 gram of the mixture from step 2. 5. Mix the mixture from step c with 100 ml of 0.5% chitosan by weight/volume until thoroughly combined. 6. The mixture is then processed using a spray dryer with the inlet temperature set to 120-140°C. 7. The powder is then collected. 2. Manufacturing process of a prototype probiotic product for pigs according to claim 1, which involves grinding the mixture... They are small in size and dried using a spray dryer, with the optimal inlet temperature set at 130 degrees Celsius. ------19/09/2019------(OCR) Page 1 of 1. Claim 1. The process for producing a prototype probiotic product for pigs is as follows: 1. Preparation of coating materials: a. Inner coating by dissolving 1.5% w/v alginate in water, and b. Outer coating material is 0.5% w/v chitosan dissolved in 0.1% w/v acetic acid. 2. Probiotic coating: c. Inner coating with alginate, using probiotic cultures at a concentration of 10⁹ colonies per milliliter. 1. Mix with 1.5% alginate by weight/volume in a 1:5 ratio. 2. The mixture from step c is then finely ground and dried using a spray dryer with the inlet temperature set to 120-140°C, with the optimal temperature being 130°C. 3. Encapsulation with chitosan. 3. Encapsulation with chitosan. 4. Take 1 gram of the mixture from step 2. 5. Mix the mixture from step c with 100 ml of 0.5% chitosan by weight/volume until thoroughly combined. 6. The mixture is then processed using a spray dryer with the inlet temperature set to 120-140°C. 7. The powder is then collected. 2. Manufacturing process of a prototype probiotic product for pigs according to claim 1, which involves grinding the mixture... They are small in size and dried using a spray dryer, with the optimal inlet temperature set at 130 degrees Celsius. ------------ Edited 06/11/2018 No claims ---------- Page 1 of 1 Claims 1. The prototype probiotic product for pigs has the following process: 1. Preparation of coating materials: a. The inner coating is made by dissolving 1.5% w/v alginate in water, and b. The outer coating material is 0.5% w/v chitosan dissolved in 0.1% w/v solution. 2. Probiotic inoculation: a. Inner coating with alginate: Probiotic cultures at a concentration of 10⁹ colonies per milliliter are mixed with the alginate. 1. 1.5% weight/volume in a 1:5 ratio. b. Take the mixture from step a and dry it using a spray dryer, setting the inlet temperature to 120-140°C. c. Collect the powder obtained from the machine. 3. Outer layer coating with chitosan. a. Take 1 gram of the mixture from step 2. b. Mix the mixture from step a with 100 ml of chitosan at a concentration of 0.5% weight/volume until thoroughly combined. c. Process the resulting mixture using a spray dryer, setting the inlet temperature to 120-140°C. d. Collect the powder. 2. Prototype probiotic product for pigs, pursuant to claim paragraph 1, where the solvent of the outer coating material, chitosan, is acetic acid. 3. A prototype probiotic product for pigs, pursuant to claim clause 1 or 2, where the mixture is finely ground and dried using a spray dryer process, with the optimal inlet temperature being 130°C.