TH14801A3 - Extraction method of inter-receptor compounds from cyclosorus teminans ferns. - Google Patents

Abstract

------ 29/03/2018 ------ (OCR) page 1 of the number 1 page invention summary. The invention involved a method of extracting an extract from a cyclosaurus fern to obtain an extract of fern. That is rich in substances from the Inter receiving soil A B and C in high quantity Which consists of raw material preparation process, solvent extraction Solvent extraction in combination with the use of a sonikator Solvent extraction in combination with Use a microwave And solvent extraction in conjunction with the use of a reflux machine According to the state that was invented ------------ ------ 31/08/2017 ------ (OCR) Page 1 of the number 1 page Summary of invention The invention involved a method of extracting an extract from a cyclosaurus fern to obtain an extract of fern. That is rich in substances from the Interrtin A group B and C in high quantity Which consists of raw material preparation process, solvent extraction Solvent extraction in combination with the use of a sonikator Solvent extraction in combination with Use a microwave And solvent extraction in conjunction with the use of a reflux machine According to the invented state ------------ This invention relates to the extraction method of ferns of cyclosaurus terminates to obtain fern extracts. That is rich in substances from the Interrtin A group B and C in high quantity Which consists of raw material preparation process, solvent extraction Solvent extraction in combination with the use of a sonikator Solvent extraction in combination with Use a microwave And solvent extraction in conjunction with the use of a reflux machine According to the condition that was invented

Claims (1)

Disclaimer (all) which will not appear on the advertisement page. : ------ 29/03/2018 ------ (OCR) Page 1 of 2 pages Disclaimer 1. Process of extraction of interstitin from fern cyclosaurus Minans (Cyclosorus terminans) follow this invention. The method is as follows: Step 1: Raw material preparation, starting with the above ground part of the fern cyclosaurus terminates, cleaning and drying by baking or drying at temperature 50-60 ° C and Minimize the size with a grinder. To obtain dry fern powder. Step 2 Solvent extraction. The dried fern powder obtained from step 1 is added to the polar solution organic solvent, namely ethyl acetate, isopropanol or ethanol in the ratio of fern powder (g ) Per solvent (mL) in the range 1:20 to 1: 5. The samples were put into a shaker so that the solvent was thoroughly exposed to the sample at a rotation rate of 100-300 rpm (rpm) for 48-72 hours at 25-40 ° C. The lead time is secondary only to keep the solution. This will extract from Fern A. and the remaining fern residue to be re-extracted using the same method for 2-5 cycles. Step 3 Solvent extraction with the use of a sonicator. Solvent extraction The fern residue from the extraction in step 2 was added to the polar solution organic solvent, namely ethyl acetate or isopropanol or ethanol. The solution containing the sample is then placed in A sonicator at a frequency of 37 kHz for 20-30 minutes at a temperature of 30-60 ° C to make the solution move. After the expiry time, only the solution is kept, which will extract the fern B. and the remaining fern residue to be re-extracted by the same method for 2-5 cycles. Step 4 Solvent extraction. Together with the use of a microwave Will bring the fern residue from Extracted from step 3 to add organic solvent that is polar solution, namely ethyl acetate or isopropanol or ethanol. The solution containing the sample is then placed in a microwave for 2-5 minutes at 400-800 watts, and let it cool when the extra leading time is reached. Solution stored The extract from fern C. and the remaining fern residue were extracted by the same method for 2-5 cycles. Step 5 Solvent extraction with the use of a reflux machine. The fern residue from the extraction step 4 was added to the polar solution organic solvent, namely ethyl acetate or isopropa. Nol or ethanol Then, the solution containing the said sample was placed in a reflux machine at temperature 70-80 p. 2 of 2 p. C for 30-60 min. From fern D. and the remaining fern residue was extracted by the same method for 2-5 cycles. Step 6 Mixing the extract obtained from steps 2-5, in which this step will bring the solution a. B and d from step 2-5 are mixed together and dried with evaporator under vacuum (evaporator) at 40-50 ° C until rough extract of fern ------- ----- ------ 31/08/2017 ------ (OCR) Page 1 of 2 pages Disclaimer 1. Extraction method of intertin from fern cyclosol group. Rust Terminans (Cyclosorus terminans) follow this invention. The method is as follows: Step 1: Raw material preparation, starting with the above ground part of the fern cyclosaurus terminates, cleaning and drying by baking or drying at temperature 50-60 ° C and Minimize the size with a grinder. To obtain dry fern powder. Step 2 Solvent extraction. The dried fern powder obtained from step 1 is added to the polar solution organic solvent, namely ethyl acetate, isopropanol or ethanol in the ratio of fern powder (g ) To the solvent (ml) in the range 1:20 to 1: 5, then the solution containing The sample is then placed in a shaker so that the solvent is thoroughly exposed to the sample at a rotation rate of 100-300 rpm (rpm) for 48-72 hours at 25-40 ° C. The lead time is secondary only to keep the solution. This will extract from Fern A. and the remaining fern residue to be re-extracted using the same method for 2-5 cycles. Step 3 Solvent extraction with the use of a sonicator. Solvent extraction The fern residue from the extraction in step 2 was added to the polar solution organic solvent, namely ethyl acetate or isopropanol or ethanol. The solution containing the sample is then placed in A sonicator at a frequency of 37 kHz for 20-30 minutes at a temperature of 30-60 ° C to make the solution move. After the expiry time, only the solution is kept, which will extract the fern B. and the remaining fern residue to be re-extracted by the same method for 2-5 cycles. Step 4 Solvent extraction. Together with the use of a microwave Will bring the fern residue from Extracted from step 3 to add polar solution organic solvent such as ethyl acetate or isopropanol or ethanol. The solution containing the sample is then placed in a microwave for 2-5 minutes using the power at 400-800 watts, let cool at the end of the lead time, only secondary Solution stored The extract from fern C. and the remaining fern residue were extracted by the same method for 2-5 cycles. Step 5 Solvent extraction with the use of a reflux machine. The fern residue from the extraction step 4 was added to the polar solution organic solvent such as ethyl acetate or isopropanol or ethanol, then the solution containing the sample It was cleared in the reflux machine at a temperature of 70-80 degrees, page 2 of the amount of 2 pages Celsius for 30-60 minutes. The remainder was extracted by the same method for 2-5 cycles. Step 6. Mixing the extract obtained from step 2-5, in which the solution A, B, C and D were taken from the step. 2-5 mixed together and dried with evaporator under vacuum (evaporator) at temperature 40-50 ° C to get a coarse extract of fern ------------ 1. The extraction process of interstitin groups from cyclosorus terminons is based on this invention. The methods are as follows: Step 1: Raw material preparation, starting with the above ground part of the fern cyclosaurus terminates, cleaning and drying by drying or drying at temperature 50-60 degrees Celsius. And reduce the size to be smaller with a grinder To obtain dry fern powder. Step 2 Solvent extraction. The dry fern powder obtained from step 1 will be added to the solvent. A polar solution of organic matter is ethyl acetate, isopropanol or ethanol. The ratio of fern powder (g) to solvent (millidr) ranged from 1:20 to 1: 5. The sample is then placed in a shaker so that the solvent is thoroughly exposed to the sample at a rotation rate of 100-300 rpm for 48-72 hours at 25-40 ° C. Secondary took only the solution stored. The extracts from Fern A. and the remaining fern residue were extracted by adding 2-5 cycles. Step 3 Solvent extraction in conjunction with the use of a resonator. Solvent Extraction: Fern residue from the extraction step 2 was added to the polar solution of organic solvent. Including ethyl acetate or isopropanol or ethanol The sample solution is then placed in a sonicator for 20-30 minutes at a temperature of 30-60 ° C to form a solution. The movement When the very lead time is over, only the solution is kept. Which will extract from fern B. and the remaining fern residue to be extracted by the same method for 2-5 cycles. Step 4 Solvent extraction with the use of a microwave. The fern residue from the extraction from step 3 is added to the polar solution organic solvent such as ethyl acetate or isopropanol or ethanol. The sample was placed in a microwave for 2-5 minutes using 400-800 watts power and left to cool at the end of the long lead time, only after the solution was kept. The remaining fern residue was re-extracted by the same method for 2-5 cycles. Step 5 Solvent extraction in conjunction with a reflux machine. The fern residue from the extraction step 4 is added to the polar solvent organic solvent such as ethyl acetate or isopropanol or ethanol, then the solution containing the sample. The aforementioned solution was put in the reflux machine at 70-80 degrees Celsius for 30-60 minutes. Subsequently, only the solution was stored so that the extract from fern D. and the remaining fern residue were extracted again By the same method for 2-5 cycles. Step 6 Mixing the extract obtained from step 2-5, in which this step will mix the solution AB, C and D from step 2-5. Together and dried with evaporator under vacuum (evaporator) at temperature 40-50 degrees Celsius until it is a rough extract of fern.