TH11910Y - Method for preparing passion fruit seed extract. - Google Patents

Abstract

Revised on February 19, 2016: The invention provides a method for preparing passion fruit seed extract, which can be prepared by grinding passion fruit seeds to a smaller size, followed by refluxing the resulting seeds with a solvent to obtain a mixture containing the passion fruit seed extract, and then purifying or separating it with the organic solvents hexane and then ethyl acetate (EtOAc), respectively, to obtain a passion fruit seed extract with antioxidant and sun-protective properties. ---------------------------------------------------------------------------------- Revised on November 10, 2015: The invention provides a method for preparing passion fruit seed extract to obtain a compound containing passion fruit seed extract with effective antioxidant and sun-protective properties, and for use as an ingredient in cosmetics for sun protection. ------------------------------------------------------------------------------ New request revised on July 25, 2016: The invention provides a method for preparing passion fruit seed extract, which can be prepared by grinding passion fruit seeds to a smaller size, followed by refluxing the resulting passion fruit seeds with a solvent to obtain a mixture containing the passion fruit seed extract, and then purifying or separating it with the organic solvents hexane and then ethyl acetate (EtOAc), respectively, to obtain a passion fruit seed extract with antioxidant and sun-protective properties, and for use as an ingredient in cosmetics for sun protection. Hexane and then ethyl acetate (EtOAc), respectively, were used to obtain passion fruit seed extracts with antioxidant and sun-protective properties.

Claims (3)

Revised 10/11/2015 1. The preparation process for passion fruit seed extract involves the following steps: a. Preparation of passion fruit seeds by drying using heat. The optimal temperature for drying fresh seeds is 40-50°C for 20 hours before grinding the seeds to reduce their size. b. Refluxing the passion fruit seeds. The passion fruit seeds from step a are mixed with an alcoholic solvent (methanol or ethanol) at a concentration of 40%, at a ratio of 1:1 to 1:7.5 by weight to volume. c. Purification or separation using organic solvents: - First, the solution from step b is mixed with hexane solvent, and the solution is separated into two parts: the passion fruit seed extract in the hexane solvent and the extract itself. And the remaining solution - Step two: Mix the remaining solution from step one with ethyl acetate (EtOAc) solvent and separate the solution into two parts: passion fruit seed extract in ethyl acetate (EtOAc) solvent and passion fruit seed extract in water. D. Evaporate the passion fruit seed extract in ethyl acetate (EtOAc) solvent to dryness. The mixed solution will yield a solid passion fruit seed extract product with a yield of 1.0 – 1.5% weight/weight of the starting material. 2. The method for preparing passion fruit seed extract according to claim 1, where the passion fruit seeds can be selected from fresh seeds, dried seeds, or seeds that have undergone a drying process. 3. The method for preparing passion fruit seed extract according to claim 1, where the optimal ratio of passion fruit seeds to solvent is 1:3 weight/volume. -------------------------------------------- Revised 19/02/2016 1. The method for preparing passion fruit seed extract has the following steps: A. Preparation of passion fruit seeds by drying process. A. Drying the fresh seeds: The optimal temperature for drying is 40-50°C for 24 hours before grinding the passion fruit seeds to reduce their size. B. Refluxing the passion fruit seeds: Mix the collected passion fruit seeds with an alcoholic solvent (methanol or ethanol) at a concentration of 40%, at a ratio of 1:1 to 1:7.5 by weight to volume. C. Purification or extraction with organic solvents: First, mix the solution from step B with hexane solvent and separate the solution into two parts: the passion fruit seed extract in the hexane solvent and the rest of the solution. And the remaining solution - Step two: Mix the remaining solution from step one with ethyl acetate (EtOAc) solvent and separate the solution into two parts: passion fruit seed extract in ethyl acetate (EtOAc) solvent and passion fruit seed extract in water. D. Evaporate the passion fruit seed extract in ethyl acetate (EtOAc) solvent to dryness. The mixed solution will yield a solid passion fruit seed extract product with a yield of 1.0 – 1.5% weight/weight of the starting material. 2. The method for preparing passion fruit seed extract according to claim 1, where the passion fruit seeds can be selected from fresh seeds, dried seeds, or dried seeds. 3. The method for preparing passion fruit seed extract according to claim 1, where the optimal ratio of passion fruit seeds to solvent is 1:3 weight/volume. ------------------------------------------------------- New application revised 25/7/2016 1. The preparation process for passion fruit seed extract involves the following steps: a. Preparation of passion fruit seeds by drying using heat. The optimal temperature for drying fresh seeds is 40-50°C for 24 hours, after which the seeds are ground to reduce their size. b. Refluxing the passion fruit seeds. The seeds from step a are mixed with an alcoholic solvent (methanol or ethanol) at a concentration of 40%, at a ratio of 1:1 to 1:7.5 by weight to volume. c. Purification or separation using organic solvents: - First, the solution from step b is mixed with hexane solvent, and the solution is separated into two parts: the passion fruit seed extract in the hexane solvent and the extract in the hexane solvent. And the remaining solution - Step two: Mix the remaining solution from step one with ethyl acetate (EtOAc) solvent and separate the solution into two parts: passion fruit seed extract in ethyl acetate (EtOAc) solvent and passion fruit seed extract in water. Evaporate the passion fruit seed extract in ethyl acetate (EtOAc) solvent to dryness. The resulting mixture will yield a solid passion fruit seed extract with a yield of 1.0 - 1.5% by weight of the starting material. 2. Method for preparing passion fruit seed extract according to Patent 1, where passion fruit seeds can be selected from fresh seeds, dried seeds, or dried seeds. 3. The method for preparing the passion fruit seed extract according to Patent 1, where the optimal ratio of passion fruit seeds to solvent is 1:3 weight/volume.