TH12018Y - Method for preparing powdered extract from the bark of Melaleuca leucadendron. - Google Patents

Abstract

Revised Invention Summary 25/08/2016 (23/08/2016) The process for preparing powdered extract from Melaleuca religiosa bark involves the following steps: The Melaleuca religiosa bark is ground to a powder, soaked in water, and then squeezed to remove excess water. The powder is then extracted using ethyl alcohol by the percolation method. The concentrated alcohol extract is then evaporated using a low-pressure evaporator. This develops a method for preparing Melaleuca religiosa bark extract for use in herbal medicine or cosmetic products. The method uses a non-thermal approach to minimize the degradation of active compounds, resulting in a simple extraction process with short extraction times and minimal harmful substances. The extract yields a high concentration (10-13%) and exhibits antioxidant activity as tested using the PPS spray and lipid peroxidation methods. Furthermore, the extract is dry, effectively inhibits microorganisms, and is non-toxic at concentrations less than 100 µg/ml. The resulting extract is a fine, dry powder that does not absorb moisture and possesses good physical characteristics suitable for the development and production of pharmaceutical or cosmetic products. ------------------------------------------------------- Preparation Method for Powdered Extract from Melaleuca leucadendron bark: This invention describes a method for preparing a high-concentration, highly bioactive extract from Melaleuca leucadendron bark for use in herbal medicine or cosmetic products. The developed extraction process utilizes a non-thermal method to minimize degradation of active compounds. The extraction steps are simple, time-efficient, and use minimally harmful substances. The extraction aids are uncomplicated and suitable for large-scale industrial applications. The resulting extract exhibits a high concentration of active compounds (10-13%) and demonstrates antioxidant activity using PPS-S spray and lipid peroxidation methods. Furthermore, the extract is dry, exhibits good antimicrobial activity, and is non-toxic at concentrations less than 100 µg/ml. The extract is a fine, dry powder that does not absorb moisture and possesses excellent physical characteristics, making it suitable for the development and production of pharmaceutical or cosmetic products.

Claims (1)

Revised 23/06/2016 1. The process for preparing powdered extract from Melaleuca leucadendron bark is as follows: a. Dry the bark of the Melaleuca leucadendron tree by air drying, sun drying, or oven drying at a temperature between 50 and 55 degrees Celsius. b. Grind the dried bark from step a into a powder to increase the surface area. c. Soak the powdered bark in water for 10-15 minutes to allow it to swell, enabling the solvent to easily penetrate and extract the substances from the plant powder. Squeeze out the excess water until it is almost dry. d. Extract the powdered bark using ethyl alcohol by the percolation method. Ferment the powdered bark with ethyl alcohol. (80-95% ethyl alcohol concentration) 3-5 times the weight of the white mangrove bark powder, 15 minutes each time. Circulate the ethyl alcohol 3 times, then replace with new alcohol and repeat the extraction method. Repeat this process a total of 3 times to extract the active compounds from the mangrove bark. Combine the extracts obtained from step d and filter through filter paper to separate powder particles from the alcohol extract. Dry the concentrated alcohol extract using a low-pressure evaporator at 60°C. Weigh the extract to calculate the percentage yield and store samples at 2-8°C. -------------------------------------------------------------- Revised 25/08/2016 (23/08/2016) 1. The process for preparing powdered extract from white mangrove bark is as follows: A. Take the bark of the white mangrove trunk and dry it by air drying, sun drying, or oven drying. a. The temperature should be between 50 and 55 degrees Celsius. b. Grind the dried white mangrove bark obtained from step a into a powder to increase the surface area. c. Soak the white mangrove bark powder in water for 10-15 minutes to allow it to swell, enabling the solvent to easily penetrate and extract the substances from the plant powder. Squeeze out the excess water until it is almost dry. d. Extract the white mangrove bark powder using ethyl alcohol by the percolation method. Soak the white mangrove bark powder in ethyl alcohol (80-95% concentration) at a concentration 3-5 times the weight of the powder, for 15 minutes each time. Circulate the ethyl alcohol three times, then replace with fresh alcohol and repeat the extraction process a total of three times to extract the active compounds. J. Extract the alcohol from the bark of the Melaleuca tree. K. Combine the extracts obtained from step D and filter them through filter paper to separate the powder from the alcohol extract. F. Dry the concentrated alcohol extract using a low-pressure evaporator at 60°C. G. Weigh the extract to calculate the percentage yield and store samples at 2-8°C. 1. The process for preparing powdered extract from Melaleuca leucadendron bark is as follows: a. Dry the bark of the Melaleuca leucadendron tree by air drying, sun drying, or oven drying at a temperature between 50 and 55 degrees Celsius. b. Reduce the particle size of the dried bark to increase the surface area. c. Soak the powdered Melaleuca leucadendron bark in water for at least 10-15 minutes to allow the powder to expand, facilitating the penetration of the solvent into the plant material. Squeeze out the excess water. d. Extract the powdered Melaleuca leucadendron bark using ethyl alcohol by the percolation method. The powdered Melaleuca leucadendron bark is then fermented with ethyl alcohol. (Ethyl alcohol concentration 80-95%) Use at least 3-5 times the weight of the white mangrove bark powder. Extract for 15 minutes each time, swirling the ethyl alcohol at least 3 times, then replace with fresh alcohol and repeat the extraction process. Repeat this process a total of 3 times to extract the active compounds from the mangrove bark. Filter the obtained extract using filter paper to separate the powder from the alcohol extract. Combine the extracts obtained from step 4. Concentrate the alcohol extract using a low-pressure evaporator, then dry the concentrated extract at a temperature not exceeding 60°C. Weigh the extract to calculate the percentage yield and collect samples at 2-8°C.