CN104397149B - A kind of method of employing Sorbus sibirica extracting solution inhibition of potato sprouting - Google Patents
A kind of method of employing Sorbus sibirica extracting solution inhibition of potato sprouting Download PDFInfo
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- CN104397149B CN104397149B CN201410630103.6A CN201410630103A CN104397149B CN 104397149 B CN104397149 B CN 104397149B CN 201410630103 A CN201410630103 A CN 201410630103A CN 104397149 B CN104397149 B CN 104397149B
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- extracting solution
- rhizoma solani
- solani tuber
- tuber osi
- sorbus sibirica
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Abstract
The present invention relates to a kind of method of employing Sorbus sibirica extracting solution inhibition of potato sprouting, the method utilizes Sorbus sibirica branch, it is prepared into extracting solution according to a conventional method, then extracting solution is sprayed on Rhizoma Solani tuber osi, so as to reaches the germination for suppressing storage period Rhizoma Solani tuber osi.Extracting solution environmental protection prepared by the Sorbus sibirica branch that the present invention is adopted, pollution-free, nonhazardouss, do not produce pollution to fruit and vegerable, the mankind and environment, draw materials easily, if extract is made wettable powder and with reference to the administration of other biostatic agents, the effect of sprout inhibition can be more preferably.Sorbus sibirica extracting solution of the present invention is except can be in addition to inhibition of potato sprouting, there is significant inhibitory action to Semen Tritici aestivi and barley germination, also have simultaneously and effectively press down mycostatic growth, reduce the generation of canker, this method is applied to the drier region of weather.
Description
Technical field
The present invention relates to a kind of method of employing Sorbus sibirica extracting solution inhibition of potato sprouting, specifically west primary
Leah Fructus Sorbi Pohuashanensis（Sorbus sibirica）Branch extracting solution is inhibited to potato sprouting.
Background technology
Rhizoma Solani tuber osi（Solanum tuberosum L）The third-largest important cereal crops in the whole world, Chinese five big staple foods it
One.About 80,000,000 mu of China's cultivated area, annual production is up to more than 8,300 ten thousand tons.Which is of high nutritive value, adaptive faculty strong, because which is ensureing
More and more important effect has been played in terms of grain security and has obtained the extensive attention of government department.Eat as a kind of winter storage
Crop, should need to be extended from the storage period for harvesting longer edible period, but Rhizoma Solani tuber osi may send out during this
Hair growth promoting bud trend, this always perplex one of topmost problem of Development of Potato Industry.This germination may result in a certain degree
Addle, affect Rhizoma Solani tuber osi quality and commodity rate.But this is avoidable.Show there is substantial amounts of chemistry according to documents and materials
Preparation can inhibition of potato germination（Chlorpropham, chloronitrobenzene）, but they have various shortcomings, and life-time service is easy
Induction is developed immunity to drugs, and pollutes environment, destroys the ecological balance of microorganism species.The pesticide of residual not only affects the matter of agricultural product
Amount, and endanger human health.The research of Sorbus sibirica is shown, its branch and leaf, bark and timber contain antipathogenic composition,
There is very strong germination rejection, than at present to Rhizoma Solani tuber osi using material more have a lot of advantages.
Literature search is disclosed：Life Science and Technology College, Xinjiang Univ.'s iwan Gu is beautiful. and Ma Hemu is carried et al. in " western Berli
In the measure of sub- Fructus Sorbi Pohuashanensis branch extract bacteriostasis ", the measure of Sorbus sibirica branch extract is shown, extract pair
Antibacterial and funguses have obvious inhibitory action, and after 121 DEG C of humid heat treatments 30min, inhibitory effect declines less, with good
Heat stability and ultraviolet light stability, its bacteriostasis strengthen with the prolongation of antibacterial time.With regard to inhibition of potato sprouting
Using having no report.
The suppression of germination refers to that bud number or reduction bud on every piece of Rhizoma Solani tuber osi of minimizing are long.For this purpose, the invention provides a kind of
Using the method for Sorbus sibirica extracting solution inhibition of potato sprouting, the method is existed using Sorbus sibirica branch extracting solution
The germination of storage period inhibition of potato.
Sorbus sibirica（Sorbus sibirica）It is Rosaceae Maloideae Fructus Sorbi Pohuashanensis platymiscium, is a kind of high economical
The dungarunga of value, Altai in Xinjiang Province are distributed.Literature search is disclosed：The fruit of Sorbus sibirica, blade, twig etc. are early
Just extensively apply in folk medicine, corneal is scorching to have preventive and therapeutic action, can improve human body anti-radiation ability.Long-term clothes
Internal hemorrhoid can be cured with Sorbus domestica decoction；Take the spire twig juice harvested before Fructus Sorbi Pohuashanensis bloom and can prevent and treat cholelithiasis and kidney knot
Stone.Russian peasant makes the container of various storage milk product and oil product of Sorbus sibirica timber, can preserve food for a long time
Product are unlikely to deteriorate.
Content of the invention
It is an object of the invention to, there is provided a kind of side of employing Sorbus sibirica extracting solution inhibition of potato sprouting
Method, the method utilize Sorbus sibirica branch, prepare extracting solution using conventional method, then extracting solution is sprayed in Rhizoma Solani tuber osi
On, so as to reach the germination for suppressing storage period Rhizoma Solani tuber osi.Extracting solution prepared by the Sorbus sibirica branch that the present invention is adopted
Environmental protection, pollution-free, nonhazardouss do not produce pollution to fruit and vegerable, the mankind and environment, draw materials easily, easily by numerous peasants and herdsmen
Receive, meet the requirement of current green agricultural product circulation development.If by extract make wettable powder and with combine other
Biostatic agent apply, the effect of sprout inhibition can be more preferably.
A kind of method of employing Sorbus sibirica extracting solution inhibition of potato sprouting of the present invention, follow these steps to
Carry out：
A, the preparation of Sorbus sibirica extracting solution：The western Berli that spontaneous growth is taken using conventional ethanol Soxhlet extraction method
The branch of sub- Fructus Sorbi Pohuashanensis is crushed to the granule of particle diameter 3-5mm, is mounted in the bottle of lid, and the concentration for adding 4 times of amounts is 95% second
Alcohol, the granule of crushing is fully immersed in ethanol, put quiet at room temperature put 24h, next day filters to get filtrate, and filtering residue is added
The concentration of 3 times of amounts are 95% ethanol, and in 100 DEG C of electric-heated thermostatic water bath temperature, atmospheric pressure reflux extracts 8-18 hours, filters and must filter
Liquid, merging filtrate are placed in Rotary Evaporators temperature 50-60 DEG C concentrating under reduced pressure and isolate ethanol, then by concentrated solution drying
The 40 DEG C of drying of case temperature, obtain Sorbus sibirica extracting solution concentrate, then by extracting solution concentrate in 4 DEG C of refrigerator storages of temperature
Standby；
B, storage of potato process：Choose disease, residual Rhizoma Solani tuber osi after Rhizoma Solani tuber osi is dug out, load storage place and place 40 60
My god, standby；
C, the Sorbus sibirica extracting solution concentrate for obtaining step a take 75g-175g, plus 500ml distilled water, dilution
To concentration for 15%-35% extracting solution, then will step b process after Rhizoma Solani tuber osi surface on be spaced 15-20 minutes and uniformly spray and carry
Take liquid 2-3 time, it is ensured that all eyes on every piece of Rhizoma Solani tuber osi are covered all, then are dried the Rhizoma Solani tuber osi after extracting solution is sprayed,
Preserved in the storeroom of temperature 4-8 DEG C, be compared with matched group after 4-5 month respectively, i.e. inhibition of potato sprouting
Rate is 21.6-43.6%.
Description of the drawings
Fig. 1 is variable concentrations Sorbus sibirica extracting solution of the present invention and Dutch No. seven potato sprouting suppression ratio relations
Broken line graph, wherein-◆-it is 4 months ,-■-it is 5 months；
Fig. 2 is the folding of variable concentrations Sorbus sibirica extracting solution of the present invention and pale reddish brown white potato germination suppression ratio relation
Line chart, wherein-◆-it is 4 months ,-■-it is 5 months.
Specific embodiment
Embodiment 1
Potato Cultivars Holland seven, the pale reddish brown each in vain 60kg for preparing Condition in North Xinjiang cultivation is standby；
A, the preparation of Sorbus sibirica extracting solution：The western Berli that spontaneous growth is taken using conventional ethanol Soxhlet extraction method
The branch 1kg of sub- Fructus Sorbi Pohuashanensis is crushed to the granule of particle diameter 3-5mm, is mounted in the bottle of lid, and the concentration for adding 4 times of amounts is 95%
Ethanol, the granule of crushing is fully immersed in ethanol, put quiet at room temperature put 24h, next day filters to get filtrate, and filtering residue is added again
The concentration for entering 3 times of amounts are 95% ethanol, and in 100 DEG C of electric-heated thermostatic water bath temperature, atmospheric pressure reflux is extracted 8 hours, is filtered and must be filtered
Liquid, merging filtrate, then filtrate is placed in Rotary Evaporators temperature 50 C concentrating under reduced pressure and isolates ethanol, by concentrated solution drying
The 40 DEG C of drying of case temperature, obtain Sorbus sibirica extracting solution concentrate 87.2g, yield 8.72%, then extracting solution concentrate are existed
4 DEG C of refrigerator storages of temperature are standby；
B, storage of potato process：Without cleaning after Potato Cultivars Holland seven, pale reddish brown each in vain 60kg are dug out, choose
Sick, residual Rhizoma Solani tuber osi, loads storage place and places 40 days, standby；
C, the Sorbus sibirica extracting solution concentrate for obtaining step a take 75g, plus 500ml distilled water, are diluted to concentration
For 15% extracting solution, then will step b process after Rhizoma Solani tuber osi surface on be spaced 15-20 minutes and uniformly spray extracting solution 2-3 time,
Guarantee that all eyes on every piece of Rhizoma Solani tuber osi are covered all, then dry the Rhizoma Solani tuber osi after extracting solution is sprayed, in temperature 4-8 DEG C
Storeroom in preserved, be compared with matched group after 4-5 month respectively, 4 months Potato Cultivars Holland of storage seven
The pale reddish brown poliosis bud inhibition rate 25.8% of germination suppression ratio 23.1%, Potato Cultivars；5 months Potato Cultivars of storage are Dutch No. seven
The pale reddish brown poliosis bud inhibition rate 23.4% of bud inhibition rate 21.6%, Potato Cultivars.
Embodiment 2
Prepare Potato Cultivars Holland No. seven, pale reddish brown each in vain 60kg of Condition in North Xinjiang cultivation, standby；
A, the preparation of Sorbus sibirica extracting solution：The western Berli that spontaneous growth is taken using conventional ethanol Soxhlet extraction method
Sub- Fructus Sorbi Pohuashanensis branch 1.5kg is crushed to the granule of particle diameter 3-5mm, is mounted in the bottle of lid, and the concentration for adding 4 times of amounts is 95%
Ethanol, the granule of crushing is fully immersed in ethanol, put quiet at room temperature put 24h, next day filters to get filtrate, and filtering residue is added
The concentration of 3 times of amounts are 95% ethanol, and in 100 DEG C of electric-heated thermostatic water bath temperature, atmospheric pressure reflux is extracted 12 hours, is filtered and must be filtered
Liquid, merging filtrate, then filtrate is placed in Rotary Evaporators temperature 60 C concentrating under reduced pressure and isolates ethanol, concentrated solution is used dry
The 40 DEG C of drying of dry case temperature, obtain Sorbus sibirica extracting solution concentrate 133.1g, yield 8.87%, then extracting solution are concentrated
Product are standby in 4 DEG C of refrigerator storages of temperature；
B, storage of potato process：Without cleaning after Potato Cultivars Holland seven, pale reddish brown each in vain 60kg are dug out, choose
Go out disease, residual Rhizoma Solani tuber osi, load storage place and place 50 days, standby；
C, the Sorbus sibirica extracting solution concentrate for obtaining step a take 125g, plus 500ml distilled water, are diluted to dense
The extracting solution for 25% is spent, then uniformly sprays extracting solution 2-3 by 15-20 minutes are spaced on the Rhizoma Solani tuber osi surface after the process of step b
Secondary, it is ensured that all eyes on every piece of Rhizoma Solani tuber osi are covered all, then dry the Rhizoma Solani tuber osi after extracting solution is sprayed, in temperature 4-
Preserved in 8 DEG C of storeroom, be compared with matched group after 4-5 month respectively, preserved 4 months Dutch No. seven suppression ratio
34.5%th, pale reddish brown white suppression ratio 31.6%；Storage 5 months Dutch No. seven suppression ratio 32.6%, pale reddish brown white suppression ratio 30.8%.
Embodiment 3
Prepare Rhizoma Solani tuber osi Holland No. seven, pale reddish brown each in vain 60kg of Condition in North Xinjiang cultivation, standby；
A, the preparation of Sorbus sibirica extracting solution：The western Berli that spontaneous growth is taken using conventional ethanol Soxhlet extraction method
The branch 2kg of sub- Fructus Sorbi Pohuashanensis is crushed to the granule of particle diameter 3-5mm, is mounted in the bottle of lid, and the concentration for adding 4 times of amounts is 95%
Ethanol, the granule of crushing is fully immersed in ethanol, put quiet at room temperature put 24h, next day filters to get filtrate, and filtering residue is added
The concentration of 3 times of amounts are 95% ethanol, and in 100 DEG C of electric-heated thermostatic water bath temperature, atmospheric pressure reflux is extracted 18 hours, is filtered and must be filtered
Liquid, merging filtrate are placed in 55 DEG C of Rotary Evaporators temperature concentrating under reduced pressure and isolate ethanol, by concentrated solution drying baker temperature
40 DEG C of drying of degree, obtain Sorbus sibirica extracting solution concentrate 179.6g, yield 8.98%, then by extracting solution concentrate in temperature
4 DEG C of refrigerator storages of degree are standby；
B, storage of potato process：Without cleaning after Potato Cultivars Holland seven, pale reddish brown each in vain 60kg are dug out, choose
Go out disease, residual Rhizoma Solani tuber osi, load storage place and place 60 days, standby；
C, the Sorbus sibirica extracting solution concentrate for obtaining step a take 175g, plus 500ml distilled water, are diluted to dense
The extracting solution for 35% is spent, then uniformly sprays extracting solution 2-3 by 15-20 minutes are spaced on the Rhizoma Solani tuber osi surface after the process of step b
Secondary, it is ensured that all eyes on every piece of Rhizoma Solani tuber osi are covered all, then dry the Rhizoma Solani tuber osi after extracting solution is sprayed, in temperature 4-
Preserved in 8 DEG C of storeroom, be compared with matched group after 4-5 month respectively, preserved 4 months Dutch No. seven suppression ratio
41.8%th, pale reddish brown white suppression ratio 43.4%；Storage 5 months Dutch No. seven suppression ratio 39.6%, pale reddish brown white suppression ratio 41.1%.
Used in the method for the invention, the consumption of Sorbus sibirica extracting solution becomes according to the different of Potato Cultivars
Change.Sorbus sibirica extracting solution of the present invention is except can have to Semen Tritici aestivi and barley germination in addition to inhibition of potato sprouting
There is significant inhibitory action, while also have effectively pressing down mycostatic growth, reduce the generation of canker, this method is applied to gas
Wait the region for relatively drying.
Claims (1)
1. a kind of method of employing Sorbus sibirica extracting solution inhibition of potato sprouting, it is characterised in that follow these steps to into
OK：
A, the preparation of Sorbus sibirica extracting solution：The Siberia flower that spontaneous growth is taken using conventional ethanol Soxhlet extraction method
The branch of Chinese catalpa is crushed to the granule of particle diameter 3-5mm, is mounted in the bottle of lid, and the concentration for adding 4 times of amounts is 95% ethanol, will
The granule of crushing is fully immersed in ethanol, put quiet at room temperature put 24h, next day filters to get filtrate, by filtering residue add 3 times amount
Concentration is 95% ethanol, and in 100 DEG C of electric-heated thermostatic water bath temperature, atmospheric pressure reflux extracts 8-18 hours, filters to get filtrate, and merges
Filtrate, is placed in Rotary Evaporators temperature 50-60 DEG C concentrating under reduced pressure and isolates ethanol, by concentrated solution drying baker temperature 40
DEG C dry, obtain Sorbus sibirica extracting solution concentrate, then extracting solution concentrate is standby in 4 DEG C of refrigerators storages of temperature；
B, storage of potato process：Choose disease, residual Rhizoma Solani tuber osi after Rhizoma Solani tuber osi is dug out, load storage place and place 40 60 days,
Standby；
C, the Sorbus sibirica extracting solution concentrate for obtaining step a take 75g-175g, plus 500ml distilled water, are diluted to dense
The extracting solution for 15%-35% is spent, then uniformly sprays extracting solution by 15-20 minutes are spaced on the Rhizoma Solani tuber osi surface after the process of step b
2-3 time, it is ensured that all eyes on every piece of Rhizoma Solani tuber osi are covered all, then dry the Rhizoma Solani tuber osi after extracting solution is sprayed, in temperature
Preserved in the storeroom of 4-8 DEG C of degree, be compared with matched group after 4-5 month respectively, be i.e. inhibition of potato sprouting rate is
21.6-43.6%.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1045906A (en) * | 1989-03-11 | 1990-10-10 | 南朝鲜科学技术研究院 | The method of a large amount of productive manpower seed potatos (potato microtubers) |
| CN1048784A (en) * | 1989-06-27 | 1991-01-30 | 罗纳-普朗克农业化学公司 | Inhibition of potato sprouting |
| CN101999451A (en) * | 2010-11-04 | 2011-04-06 | 国家农产品保鲜工程技术研究中心（天津） | Citronella essential oil sprout inhibitor and application thereof to potato preservation |
| CN102626134A (en) * | 2012-03-28 | 2012-08-08 | 甘肃省农业科学院农产品贮藏加工研究所 | Application of ethylene in restraining potato germination |
-
2014
- 2014-11-11 CN CN201410630103.6A patent/CN104397149B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1045906A (en) * | 1989-03-11 | 1990-10-10 | 南朝鲜科学技术研究院 | The method of a large amount of productive manpower seed potatos (potato microtubers) |
| CN1048784A (en) * | 1989-06-27 | 1991-01-30 | 罗纳-普朗克农业化学公司 | Inhibition of potato sprouting |
| CN101999451A (en) * | 2010-11-04 | 2011-04-06 | 国家农产品保鲜工程技术研究中心（天津） | Citronella essential oil sprout inhibitor and application thereof to potato preservation |
| CN102626134A (en) * | 2012-03-28 | 2012-08-08 | 甘肃省农业科学院农产品贮藏加工研究所 | Application of ethylene in restraining potato germination |
Non-Patent Citations (2)
| Title |
|---|
| 花楸树果实浸泡液的萌发抑制作用;吴超 等;《中国农学通报》;20070405;第23卷(第4期);第133页第3节 * |
| 西伯利亚花楸枝条提取物抑菌作用的测定;帕提古丽·马合木提 等;《食品科学》;20061115;第27卷(第11期);第185-188页 * |
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