SU900176A1 - Decamin determination method - Google Patents

Description

one

The invention relates to the analytical chemistry of organic compounds and can be used for the qualitative and quantitative determination of the medicinal product decamine-1, 10-decamethylene-bis- (4-aminoquinaldinium-chloride).

Dekamin is a bischetvertic ammonium compound and has antibacterial and fungicidal activity.

A known method for determining decamine, based on measuring the absorption spectrum of a solution of decamine in the ultraviolet region of the spectrum of Cl.

The disadvantage of this method is its non-selectivity V

The closest to the invention to the technical essence is the method of determining the decamine by treating an aqueous solution of the analyte with sodium acetate, applying a drop of solution on a filter paper moistened with a solution

p-dimethylaminobenzaldehyde to obtain a colored spot .P

The disadvantage of this method is its low sensitivity and non-selectivity (500 µg 8 drop, if you take a drop volume of 0.03 ml or 17 mg / ml).

The purpose of the invention is to increase the sensitivity and selectivity of the method.

to

This goal is achieved by the fact that, according to the method for determining decamine, an aqueous solution of an analyte is treated with eosin at a pH of 3, followed by photometry of the obtained colored compound.

Eosin-2, 5,7-tetrabromfluorescein has an Omeglographic O (yellow-orange color) ..

20

This method allows the determination of microgram amounts of dekamine, which is selective, since it is possible to determine it in the presence of other medicinal substances derived from four-ammonium bases (benzamon, prozerin, naphtamon). Sensitivity - O, µg / ml Example. 12.5 mg of the drug Decamine is dissolved in distilled water under heating and the volume of the solution is adjusted to 25 ml. To 0.2 ml of the resulting solution was added 0.5 ml of a solution of eosin (sodium salt), 2.5 ml of acetone and 3 ml of acetate buffer solution (pH 4.0) and brought to 25 ml with distilled water. If a decamine is present in the test preparation, the solution under these conditions acquires a red-violet color. To quantify the decamine, take an aliquot portion of the analyzed solution, add

Determined DK, mcg / ml

DK was introduced, µg / ml

0.39 0.41 0.40 0.41 0.40

Relative relative error,%

± 2.50 the above amounts of eosin acetone, buffer solution and water bring the volume of the solution to 25 ml. Optical density is measured on a spectrophotometer at 530 nm or on a photoelectrocolorimeter, for example, on FEK-56 (optical filter No. 6). It is possible to measure the optical density on other types of photoelectric colorimeters using light filters with a wavelength of 520-5 +0 nm. The content of decamine in the solution is determined from a calibration graph. Calibration solutions of the 4th graph are prepared similarly with a known decamine content from 0 to 3 µg / ml. In this concentration range of decamine, a straight linear dependence of the optical density of solutions on the concentration of decamine is observed. The results of the analysis of solutions of decamine (DC) are given in the table.

0.82 0.80

0.79 0.82 0.81 1.61 1.62 1.58 1.63 1.62

± 1.98

Claims (1)

± 1.43 5 Photometric. Determination of dekamina with eosin does not interfere with organic compounds (benzamon, quaterone, naphtamon, pentamine, benzogeksonium, metacin, prozerin) similar in structure, since they do not form colored compounds with eosin. Claim method for determining decamine, characterized in that 00176 4 in order to increase the sensitivity and selectivity of the method, the aqueous solution of the analyte is treated with eosin at pH 3 followed by photometric measurement of the obtained colored compound,. Information sources,. taken into account during the examination 1. State Pharmacopoeia, 1968, X edition, p. 230 (prototype).