SU606553A3 - Method of obtaining microorganism biomass - Google Patents

Description

ba, having a cylindrical lower section 2, passing into a conical upper section 3. As a result, there are two sections in the fermenter (internal and external; along which the flow of the nutrient medium rises and descends.

The fermenter is connected to the nutrient supply system by conduit 4 and to the gas supplying system by conduit 5. The upper portion of the fermenter is connected to the lower conduit 6, which serves to return the carbon dioxide released during the process. Pipeline 7 is provided for removal of excess gas, and pipe 8 is provided for removal of the finished wort.

A fermenter of this type serves to carry out the process of an alcoholic stalk, for the realization of which a wort is used, which is diluted molasses and containing 17% of fermentable sugars.

In the process, the wort is introduced into the fermenter of uninterrupted flow through conduit 4. The gas (oxygen) entering through conduit 5 is blown into the lower part of the upstream circulation pipe. Under its action, the gas-liquid mixture rises in an upward flow through the circulation pipe, and during the passage of the cone-shaped upwardly extending section 3, the flow velocity drops. Coming out of this pipe, the mixture descends in a downward flow. The gas formed during the fermentation process through line 6 from the upper part of the fermenter enters the bottom. Excess carbon dioxide is removed from the fermenter through line 7. By adjusting the amount of oxygen fed to the fermenter, as well as its speed, the required circulation rate of the gas-liquid mixture is set, which In turn, it provides the possibility of producing in quantities of microorganisms (yeast) or alcohol in the desired volume.

Alternatively, the fermenter has a tapering centrally installed tube 9 that tapers upwards. In this case, the channel for the upward flow of the gas-liquid mixture is the space formed by the inner surface of the body 10 of the fermenter and the tube, and for the downstream is the space formed pipe 9. Air is blown into the lower part of the channel for an upward flow of the mixture. The design of the pipe 9 provides for a slowing downward flow rate from a height of 3.0 m.

Example. To reveal the advantages of the proposed method and the known method, yeast is grown in a fermenter

(figure 2) with a diameter of 11.2 m with a continuous supply of 80 nutrient medium.

The latter is prepared from beet or sugar molasses or their mixture.

The wort is enriched with mineral nitrogen and phosphoric acid or soluble phosphate. Mineral nitrogen is introduced in the form of sulfate, phosphate, ammonia, urea, the pH of the medium is maintained at 5.0.

When working by a known method, the wort is diluted to a content of 25 g / l of sugar reducing suckers; 42,500 m UH of atmospheric air is blown in.

S Horizontal part, correspondingly. The blowing zone is .25.2 m, i.e. 1 m is 1700 m blown air per hour.

In the fermenter, the liquid level (static) is 4 m, which corresponds to 400 m of the treated liquid.

If the volume W of the liquid in the fermenter is divided into the clock volume V of the introduced wort, then we get the reciprocal dilution value / U, i.e. ,

and called the passage of time.

For case 3 described

, 400 m h

// 5 o'clock.

80 m

0

When working by a known method, 1 t / h of 92% yeast is obtained. The speeds of the ascending jets (Figs. 3a) increase and reach 0.9 m / s at the top point.

When working on the proposed method blows 2300 m / m per hour of air. Its total amount is 57SOO m / h. The volume of the nutrient medium is the same, i.e. 80 m / g, and so0

keeping reducing sugars in

it is 50 g / l.

The speed of the upstream, starting from 3 m level, is slowed down and adjusted to 0.6 m / s. Thus, the wort is given

5, the time required for the contact of developing microorganisms with gaseous and liquid substances in it. This provides, in turn, an increase in yeast yield and

They can be brought up to 2 t / g (92%) in this example, i.e. an increase of two times compared with the work by a known method.

Coefficient value / (time

55

can be reduced if

passing

the volume of the fermenter is changed by allocating dead space of zone 11. This, in turn, will ensure the intensification of the process.

60

Claims (3)

1. A method of obtaining biomass of microorganisms by cultivating the latter on a nutrient medium with continuous upward and downward flow of the nutrient medium in a closed loop through gas injection, characterized in that, in order to intensify the process, gas injection is carried out in the lower part of the upstream flow of the nutrient medium, while the amount of gas is 700-3500 m / m / h. 2. The method according to pl, tl and h a yusch and the fact that the speed of the upward flow of the gas-liquid mixture is slowed down to the end of the ascent to 0.3. m / s 3. The way popp. 1, 2, differing from the fact that during the cultivation of other types of gas injected is 1000-3000. Sources of information taken into account during the examination: 1. USSR patent 298130, -. cl. C 12 C 11/04, 1971. Ut.t ym N e i t e one .t t 0 “R tt b J "