SU597362A1 - Biomass obtaining method - Google Patents

Description

(54) METHOD OF OBTAINING BIOMASS

one

The invention relates to the microbiological industry, namely, to the preparation of feed protein and can be used to obtain the biomass of fungi from ethyl alcohol.

Methods are known for producing biomass on a medium containing ethyl alcohol as a carbon source by growing microorganisms of the species Rkodotoru and duTWittca to Mi codermo vitii {, as well as the yeast genus Candida and Stxcekaromuce & {2.

Protein is also poured by growing bacteria on ethyl alcohol, for which bacteria of the genus Sarctna, PseudoTnona.s {and others are used.

The use of these microorganisms is limited due to the low content of sulfur-containing amino acids and high - nucleic acids.

Mushrooms like Asp.riLger SpiEarta sp, accumulate biomass on media with glucose, ethyl alcohol or starch. The yield of absolutely dry biomass from a given carbon source was

72.0%. The content of crude protein varied within 5О, О-57/5 о. The composition of amino acids biomass of myelial fungi is not inferior to the biomass of fodder yeast of the genus Candida. .

The closest to the proposed invention is a method of obtaining vegetable products by picking mushrooms of the species PeniciEELum Tiotatum on a nutrient medium containing a nitrogen source, mineral salts, as the carbon source — molasses, potato hydrolyzate, sugar cane f4J.

Idnako. the method requires the use of "a-" no production waste, the amount of which is limited and related to the seasonality of the processing of agricultural products ..

The purpose of the invention is to obtain a complete protein product with a balanced content of nucleic acids and sulfur-containing amines.

The goal is achieved by using a strain from the PerttCttELUTTi -ndttttum species using a strain, as a carbon source - ethyl alcohol.

The proposed PertCltUum POtatum strain differs from the well-known PenuCLIium fungi in both morphological and physiological characteristics. R. notaturn. G-27, in contrast to other fungi of the notatum type, which actively assimilate starch and cellulose, these substrates are poorly hydrolyzed, but they actively assimilate sorbitol, manst, inositol, and glycerin. Famous mushrooms of the species P. nat / atum actively synthesize a complex of hydrolytic enzymes (amylase, pectinase, celdyulase). The activity of these enzymes in P. notatutn G - 27 is very weak. Due to the weak synthesis of these enzymes, P. notatum fungus practically does not break down cellulose and starch-containing carbon sources and does not grow well on substrates such as potato beet pulp, molasses.

Dash-shge on the cultivation of the fungus R. notafcum G-27 on various carbon sources are given in Table. one, /

Moreover, the biomass when growing P. -natuTn G-27 on potatoes and beet pulp includes unutilized substrates and only in small quantities the fungal mycelium itself.

The resulting - mushroom biomass surpasses in its qualities the well-known yeast and bacterial biomass obtained on ethanol. Comparative content of methionine and nucleic acids in microorganisms produced by running on ethanol. are given in table. -2

Other species of the genus RepCctE-E-tum can be used as protein producers; R. ctavCtorme, P. rog nejortl, .B. . l l & k1, P. and 10; C1a1lg, which accumulate up to 1O g / l of absolutely dry biomass C: its protein content is up to 6O%. The strain of the fungus Rypssssysit notatu-m G-27 is isolated from peat no-marsh soil. Stored in the Institute of Microbiology of the Academy of Sciences of the BSSR

The strain has the following characteristic.

Morphological signs. The colonies are globular, velvety, blue-green with a wide white edge. Sometimes the mycelium is developed in the form of a tuft in the center of the colony, as a result of which it acquires a cotton-like structure. On the reverse side of the colony there are golden yellow, yellow pigment diff (| 1diruyut in agar. Conidiophores depart from the basal weft hypha plexus, size 25O-5OO-2.5 µm, smooth, colorless with apical vertica metur, I carry.

01 their sterigm, in the beam 4-6 sterigm, {8-1O) 3 microns. Metuli 3-5 in a whorl, different in length.

Cultural features. On wort agar, numbus colonies have a diameter of about 3 cm, white, velvety, radially striated. Colonies painted lemon-yellow in color, conidial zone of blue-oelen. On the апapek medium, the average coloNIIs have a diameter of about 2 cm. The colonies are velvety, white in color, on the reverse side they are slightly yellow in color, they grow sparingly. When sowing on a dense nutrient medium, growth appears.

2-3 days By the 5-6th day, the colonies become velvety. On the reverse side, the colonies were not stained at first, after 3-4 days they became lemon yellow, and when aging they turned light brown. The pigment diffuses into the agar. When sown in a liquid nutrient medium, growth occurs after 1-2 days, and by 3-4 days a large amount of biomass accumulates, i consisting of a tightly woven mycelium. On starchy agar, potato-dextrose agar, Czapek's medium with the addition of 2% ethanol, the growth is good. Gelatin liquefies, peptonized milk. Nitrates restores weakly. PerLci-EtLUiTi notatuin G-27

3 differs from the known ijatatomaKTHBHO fungi digesting starch and cellulose, these substrates are hydrolyzed.

Relation to carbon sources: assimilates sorbitol, maltose, arabinose, fructose lactose, xylose, mannitol, inositol, glycerin. Poorly reduces the ram. and duplicate. PRI me R. In the tap water make

(wt.%): 0.15NaN03, 0.1SMNc) 2, SOt,, 0.05 KSe, 0.2KH2P04, O, 05 Mg-SOt, and OJ7 corn extract. The nutrient medium is sterilized, then 2% ethanol is added sterilely, seeded with the fungus PenuCtC

tvUiYi notaturn G-27 or other fungi of the genus RepcssS ESIT And grown on a bowl with 200 rpm, at 25-28 C for 72-96 h, or in fermenters. After cultivation, the mycelium is separated by filtering.

and dried. The dry biomass yield is 10-12 g / l or 5 ° -60 ° with respect to the carbon source used. The resulting biomass contains up to 65% protein with a low content of nucleic KHC.iOT (1.7-2.0%). The amount of methionine is 2.1-2.6%. Without the addition of corn extract or other growth factors, the biomass yield is 4, O-4.5 g / l with a crude protein content of 60-65%. Reducing the amount of ethano "a to 1; o leads to a slight decrease in the yield of biomass (3.8-4.0 g / l). The protein content in the biomass remains

Ethyl alcohol Potatoes Molasses Beetroot

Claims (4)

1. Certificate of authorization No. 448219 Cl, C 12 C And / O8 2. US patent number 3865691, cl. 19549, 1975. 3.Gornak N.N. n.d.Lim AN BSSR 1974, 3, 71. 4. The patent of England No. 133472, cl. 1973