SU985022A1 - Strain of penicillium fellutanum fungus, 57699-producent of pectinesterase - Google Patents

Description

Stasch, iPeniciPCium feeCt / anuh, 5769 taken from the soil of the Kiev region (Ukrainian SSR). KhrashgGs culture in the collection of pure cultures of the department of physiology and systematics of micromycetes of the Institute of Microbiology and Virology. D. Zabolotnogo Academy of Sciences of the Ukrainian SSR was deposited in the central museum of industrial microorganisms of the Institute of Scientific Research Institute of Genetics under the collection number of CMPM P-152. The activity of pectin-esterae in this strain is stabilized with repeated passages and agarized nutrient media. Strain, Riclc Sum fe Dangled pi 57699, characterized by the following physiopsy morphological features, Morphological features of the fungus Pen cieeutn fePEftanum Biqur f6, Investigate the jpocT of the fungus on 6 different media of different composition. The environment of Čapek Colony is limited to growing, the condition of S1 is from dense felt woven thin hyphae, convex in the central part, radially folded along the edge, with a narrow growing uneven edge (0.5 cm in the central part are whitish-gray, extremely conidiophore zone is pale grayish -green. The back side of the colony is a grayish-green color. Konkidienesee are smooth, 3fj, f move away from the dense mycelium, single confluent, occasionally with a single side branch. CtepnrM in bundles of 8-10x2.0-2, in bundles of amp -8 lanceolate 8-10x2.0-2,. about 2.5 / smooth e, mostly dull alen, connected in colonies. Wort agar Colonies are moderately growing, well developed, common flakes grow. Concentric aons grow, center-tuft-folded, flaky-shaped flakes along the edge. Cranes grow a creamy-white color (13 mm) in the center. The reverse side of the colony is dark brown. Sporulation is weak. Potato agar Colony is weakly growing, low at heartworm with uneven white edges. Body color back. Sporulation f is absent. Potato-glucose agar Colony is moderately growing, chloroform is evo-mating with concentric growth zones. In the central part of the grayish-f smoky color, convex, the growing edge of yellow-pink (3.5 mm), smooth. With age, it becomes greyish brown. Sporonoschenie good. Identical environment of čapek. Wednesday Saburo Colony densely flocculent neural, moderately growing. The color varies from pale yellow to greyish green. Grows in concentric zones. Growing edge uneven (0.5 mm), white. The reverse side of the colony is light brown. Sporulation weak. Tom Rolin Wednesday The best growth. Colony felt-. no-puschista, with radial grooves, and separate segments. The color of the colony is from light yellow-gray to dark gray. The growing edge of the colony is uneven, wide (up to S mm), cream colored. The reverse side of the colony is light brown. Sporulation abundant. The table shows the linear growth of colonies in dynamics. 598 Physiological signs of the fungus, Ffenicl evigp ffcpeutanom In jig ebylehugugsh Mushroom assimilates glucose, galactose, maltose, fructose, sucrose, starch, inulin, sorbitol, mnnnt, succinic, and malic acid, ethanol and methanol -. weakly. Poorly absorbs cellulose. Wo: Nitrate and nitrate nitrogen, especially am1 unyishy. From organic nitrogen sources, it assimilates urea, peptone, corn extract, malt sprouts. Grows in a wide range of pH 2.5-9 Aerobe. In the process of growth, acidifies pH 2,, 3. An optimum growth temperature of 26-28 ° C. Macovimal formation of an enzyme in a culture liquid with growth on a liquid nutrient medium with products with minerals and minerals under conditions of deep cultivation is observed after up to 4 hours. Fungus Pen c-JWom feeeo-tanoip, 57699, Bodoine from cellulosic and xylolitic enzymes, does not have β-galactosidase activity, does not contain acidic protein. When foamed, and passtochtane on solo agar, a high level of fermentati & Noise activity of the strain is not reduced, and cos5) an iecs for 3 years. When cultivating in a liquid medium, of a certain composition, the strain Penicieeonifeadtanoqi, 57699, synthesizes pectin esterase. Example. The seeding material was validated at 750 ml flasks on a circular rocking chair at 220 rpm for 4O h on medium containing,%: ammonium sulfate 0.7; potassium phosphate mono-mixed O, 1; magnate sulphate 0.05} pectin containing and ductf; beetroot 3KDM 4, Of course, distilled water 10O ml. The inoculum, amounting to 1% of the total volume of the medium, is added to the medium containing the same components and in the same amounts as in the preparation of the inoculum, and This medium is cultivated during the 66 hours in 75O ml flasks on a rocking chair of 22O / MEH or in production fermenters. From the culture fluid of the fungus, oiw divided by biomass and unused substrate, a farm preparation of pectin esterase is obtained by precipitating 2.5 volumes per volume of cultured extract cooled to 4 C acetone. The precipitate was separated by centrifugation at ZOOOb / min for 10 minutes and lyophilized. 22 The activity of the fungus pectinesterase enzyme preparation is determined according to a gated procedure under standard conditions (Enzyme preparations. Methods for determining the singolite activity. GOST 20263-74, State Standard of the USSR, 1975} E are expressed in microequivalent / prohydrolyzed ester bonds to the pectin molecule in 1 min The magnitude of the activity of the enzyme of the first pr-peck-anestherase of the fungus Penic-iCetum feCOotanom, 57699, equals 172.518 O, Oe (/ g. Kim content of other enzymes of the pectolytic complex of endo- and exopolygalacturonase. Thus, the activity of endopolygalactus, determined in the enzyme specified fungus according to the hotel method, is, 18,, Oesh, exopoly-lacturonase - 82, 5 units of total pectolytic activity 48, 4-50.2 u / s, At the same time, the producer of the complex of propolytic enzymes of production value, Aepercjtggos foei-Tdus, produces, in significant co-quantities, 8th endopolygalugoloneuronase (2Ob, O-80O, 0 u / g), and acopolygalacturonase (400, O- 50O, O unit / g), and in enzyme preparations -b pektofoetinid T and x 1O 1O pektofoetidnn P x, e tinesterazy activity in the complex is only 7, 0 u / g, i.e. 26-5 times lower than that of Penictee om f eMot "4H ni, 57699.1. The strain of the fungus PenicHC-fum CeSeu-fcctnum, 57699, was tested for pathogenicity in xi antibiotics of the Institute of Ukrainian Biology and Academy of Sciences of the Ukrainian SSR. Thus, the strain ("fe 0lilc nurp, 57699, compared with vevestnom, possesses an enhanced way to biosynthesis of the pectiesterase enzyme and represents a new culture with resistant fiuriologic properties". It can be used as a producer of the enzyme precursor of pectofallutanin. Formula a and burea 1 111tam of the fungus PenicVeefum $ VIG "L" yu ", 57699, (collection of the Central

 7 -9S50228

From the Mivrosuganiam and Itnpu

that VNIgenetica, collection no. No. 488850, cl, C 12 N 1/14 1 © 73. measures CMPM {-152) - macrodistinct vectorctv .2, USSR Copyright Certificate

Source of information, 5 293846, cl. C 12 N 9/14, 1970

11rv t in the prv expert ee (prstotvl).

Claims (2)

Formula and s * b 1 PenicICWum feFEirtOtyrn, 157699, (collection of the Central Museum 57699. S3 7 '985022 8 industrial microorganisms of the Institute' VNIIgenetika ', collection number TsMPMT ~ 152) - producer of optic estera. Sources of information taken into account during the examination 1. USSR author's certificate No. 488850, class C 12N 1/14 1Θ73, 2, USSR Author's Certificate ₅ No. 293846, class C 12 N 9/14, 1970 (prototype).