SU589969A1 - Method of glaucoma diagnosis - Google Patents
Method of glaucoma diagnosisInfo
- Publication number
- SU589969A1 SU589969A1 SU741996272A SU1996272A SU589969A1 SU 589969 A1 SU589969 A1 SU 589969A1 SU 741996272 A SU741996272 A SU 741996272A SU 1996272 A SU1996272 A SU 1996272A SU 589969 A1 SU589969 A1 SU 589969A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- glaucoma
- diagnosis
- tear fluid
- dopamine
- disease
- Prior art date
Links
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- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Description
(54) СПОСОБ ДИАГНОСТИКИ ГЛАУКОМЫ(54) METHOD OF DIAGNOSTIC GLAUCOMA
1one
Изобретение относитс к области медицины , в частности к офтальмологии, и предназначено дл диагностики глаукомы.The invention relates to the field of medicine, in particular to ophthalmology, and is intended for the diagnosis of glaucoma.
Известен способ диагностики глаукомы путем качественной оценки медиатора симпатина в слезной жидкости человека.A known method for the diagnosis of glaucoma by qualitative assessment of the mediator sympatin in the tear fluid of a person.
Однако известный способ недостаточно точен и позвол ет вы вить только активную форму глаукомы и неэффективен при диагностировании ранних форм.However, the known method is not accurate enough and allows detection of only the active form of glaucoma and is ineffective in diagnosing early forms.
Целью изобретени вл етс повышение точности дагностики и раннего вы влени заболевани .The aim of the invention is to improve the accuracy of diagnosis and early detection of the disease.
Это достигаетс тем, что в слезной жидкости определ ют уровень медиаторов симпатической нервной системы: адреналина, норадреналина , дофамина и их предшественника дофа - и по их концентрации став т диагноз .This is achieved by determining the level of the sympathetic nervous system mediators in the tear fluid: adrenaline, norepinephrine, dopamine, and their predecessor dof - and their concentration is diagnosed.
Способ осуществл ют следуюшим образом.The method is carried out as follows.
Слезную жидкость забирают микропипеткой из коньюнктивального мешка глаза обследуемого после вдыхани им нашатырногоThe lacrimal fluid is taken with a micropipette from the conjunctival sac of the eye of the patient after inhaling a liquid
спирта.;. ; ., alcohol.;. ; .
Собранную л идкость помеш,ают в мерную центрифужную пробирку, в которую добавл ют 20 мг динатриевой соли этилендиаминтетрауксусной кислоты, и довод т объем пробы до 10 мл фосфатным буфером (рН 6,3). После тш;ательного перемешивани рИ,пробы довод т до 8,2-8,5 раствором 1 н. аммиака, а затем провод т адсорбционную хроматографию на колонке с окисью алюмини . Элюирование с колонки провод т 0,25 н. уксуснойThe collected liquid was stirred into a measuring centrifuge tube, to which 20 mg of ethylenediaminetetraacetic acid disodium salt was added, and the sample volume was adjusted to 10 ml with phosphate buffer (pH 6.3). After the TI was mixed thoroughly, the samples were adjusted to 8.2-8.5 with a solution of 1N. ammonia, and then carried out by adsorption chromatography on a column of alumina. Elution from the column was carried out with 0.25 n. acetic acid
кислотой. Количественную и качественную оценку элюатов на присутствие адреналина, норадреналина, дофамина и их предшественника дофа провод т по флюоресценции с помошью реакции с феррицианидом кали .acid. The quantitative and qualitative assessment of eluates for the presence of adrenaline, norepinephrine, dopamine and their precursor dofe is carried out by fluorescence using reaction with potassium ferricyanide.
У лиц, больных глаукомой, наблюдаетс концентрации названных медиаторов в слезной жидкости, причем у больных активной формой наиболее снижаетс концентраци норадреналина и дофа, а на ранних стади х заболевани наиболее снижаетс концентраци дофамина и дофа.In patients with glaucoma, concentrations of these mediators in the lacrimal fluid are observed, and in patients with the active form, the concentration of noradrenaline and dof most decreases, and in the early stages of the disease, the concentration of dopamine and dof most decreases.
Способ служит более точным диагностическим тестом по сравнению с известным способом , а также позвол ет вы вл ть заболеваниеThe method serves as a more accurate diagnostic test as compared with the known method, and also permits the detection of a disease.
на ранних стади х.in the early stages.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU741996272A SU589969A1 (en) | 1974-02-13 | 1974-02-13 | Method of glaucoma diagnosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU741996272A SU589969A1 (en) | 1974-02-13 | 1974-02-13 | Method of glaucoma diagnosis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU589969A1 true SU589969A1 (en) | 1978-01-30 |
Family
ID=20575802
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU741996272A SU589969A1 (en) | 1974-02-13 | 1974-02-13 | Method of glaucoma diagnosis |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU589969A1 (en) |
-
1974
- 1974-02-13 SU SU741996272A patent/SU589969A1/en active
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