SU1205894A1 - Method of diagnosis of echinococcosis - Google Patents

Description

one

The invention relates to medicine, in particular to the diagnosis of echinococcosis.

The purpose of the invention is to improve the accuracy of diagnosis of echinococcosis.

Example. Mix 1 ml of patient's serum and 2 ml of echinococcal fluid previously obtained from patients with echinococcus during operations and stored in sealed glass ampoules. This mixture is poured into the cuvette of the chemiluminescence measurement apparatus. The cuvette with the test mixture (3 ml) is placed in an opaque, temperature-controlled chamber at 37 ° C and the chemiluminescence (CL) of the sample is measured in pulses for 10 seconds. The background value of the setup (measured in advance) is subtracted from the obtained value and the value of the intensity of the CL mixture is obtained before incubation. ChL was measured again after 20-24 h of incubation of the mixture at 37 ° C. The intensity of CL, which characterizes the degree of activity of echinococcosis, is calculated as the difference between the measured values of the intensity of CL before and after incubation of the mixture. In order to avoid illumination of exploratory samples, their sampling, storage of measurement is performed under darkened conditions.

The diagnosis of echinococcosis is considered reliable if CL increases by incubating the mixture by 30-160 counts / 10 s.

The diagnostic value of CL is found (calculated) as follows: the average deviation (error

24 54 23 15 58

2058942

ka) average CL value (41 imp / 10 s)

control (non-echinococcal) group p

.-iili il z7.2 imp / 10 s, 5 A n (n-l),

and the mean CL (1 26 imp / 10 s) of the experimental (echinococcal) group

frij, 14 counts / 10 s. The average error of the difference CL in the experimental and control groups compared

ten

values (x

W

- X

.

 126-41

 75 imp / 10 s) is defined as, "-H4n% 15.7 imp / 10 s.

The confidence factor is.

Ex., not 9L

Determining the coefficient of reliability t and on the basis of the number of degrees of freedom L, „e„ (m „-1) (Yn ,, - О 9 + 9 18, according to a known table, determine the significance of the significance (significance) of the difference (probability P,% ) comparable indicators. In this case, it is P, 1% or average confidence interval of more than 99.9%.

The diagnostic value of CL is found on the basis of the coefficient of confidence i, corresponding to the 95% confidence interval of the mean, i.e. 195 2.1.

Required intensity value CL

S., .. - L - .. 11sh1. . 2U17.5,

 29.16 30 imp / 10 s. The research results are summarized in table. 1 and 2.

Table 1

37 29 47 36 25

95 204 76 87

104

Claims (1)

METHOD FOR ECHINOCOCCOSIS DIAGNOSTICS by mixing the patient’s blood serum with echinococcal fluid and incubating the resulting mixture, characterized in that, in order to increase the diagnostic accuracy, the chemiluminescence intensity of the mixture is measured before and after incubation and when it is increased by 30-160 pulses / 10 s, compared with the original, diagnosed with echinococcosis.