SU565935A1 - Method of obtaining uratoxylase - Google Patents
Method of obtaining uratoxylaseInfo
- Publication number
- SU565935A1 SU565935A1 SU7502117038A SU2117038A SU565935A1 SU 565935 A1 SU565935 A1 SU 565935A1 SU 7502117038 A SU7502117038 A SU 7502117038A SU 2117038 A SU2117038 A SU 2117038A SU 565935 A1 SU565935 A1 SU 565935A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- uratoxylase
- obtaining
- bvk
- composition
- weight
- Prior art date
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Description
1one
Изобретение относитс к микробиологической промышленности и может быть использовано дл получе1га биологически активных вешеств, выращенных на жидкой питательной среде, например уратоксидазы из мицели Actinomyces spectes 313-152.The invention relates to the microbiological industry and can be used to obtain biologically active substances grown in a liquid nutrient medium, for example, urotoxicity from Actinomyces spectes 313-152 mycelium.
Известен способ получени уратоксидазы, предусматривающий культивирование Actitiomy665 5pec4es313-152 в аэробных услови х на ферментационной среде, содержащей мочевую кислоту и минеральные соли, с последук цим вьщелением фе13мента ±1,A known method for producing urotoxicity involves the cultivation of Actitiomy 665 5pec4es313-152 under aerobic conditions on a fermentation medium containing uric acid and mineral salts, with a subsequent enzyme ± 1,
С целью повышени выхода и актнвйостн фермента по предложенному способу в посевную и ферментационную среды ввод т гидролйэат белкововитаминного концентрата в количествах соответстветю равных 1,О-2,0% и 1,0-.2;5% по весу сухих веществ .In order to increase the yield and actinomic enzyme according to the proposed method, a proteinaceous and vitamin concentrate hydrolyte is introduced into the sowing and fermentation medium in amounts equal to 1, O-2.0% and 1.0-.2; 5% by weight of dry substances.
Способ осуществл ют следук цим образом. The method is carried out in the following manner.
Культуру Adi (tomyce specieg 313-152 выращивают в аэробных услови х на посевной и ферментационных средах, содержащих мочевую кислоту, минеральные солн и гидролизат белково-витаминного ко1щентрата (БВК) Adi culture (tomyce specieg 313-152 grown under aerobic conditions in seed and fermentation media containing uric acid, mineral suns and hydrolyzed protein-vitamin concentrate (BVK)
в количествах соответственно paBia.ix 1,О2 ,0% и 1,О-2,5% по весу сухих вешеств с последующим выделением фермента.in amounts paBia.ix 1, О2, 0% and 1, О-2,5%, by weight of dry matter, followed by release of the enzyme, respectively.
Состав ферментационной среды, вес.%:The composition of the fermentation medium, wt.%:
т БВКt BVK
1,0-2,5 (по1.0-2.5 (by
сухим вешествакdry things
кислотаacid
О,2-О,35Oh, 2, 35
0,01-0,150.01-0.15
0,01-0,070.01-0.07
0,О1-О,О70, O1-O, O7
р соста среды вход т микроэлементы Fe , СиThe composition of the medium includes trace elements Fe, Cu
Состав посевной среды, вес. %:The composition of the inoculum, weight. %:
Тидролизат БВКHydrolyzate BVK
1-2 (по сухим веществам) 1-2 (on dry substances)
, 0,01-0,07 10,01-0,07 , 0.01-0.07 10.01-0.07
jKcejKce
MgSo.,: О,О1-О,О7MgSo.,: O, O1-O, O7
р|)еда содержит микроэлементы е , Си .p |) food contains trace elements e, C.
Пример 1. Посевную культуру Acit 313-152 выращивают на среде, содержащей 1% гидролизата БВК .Example 1. Sowing culture Acit 313-152 grown on medium containing 1% hydrolyzed BVK.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU7502117038A SU565935A1 (en) | 1975-03-21 | 1975-03-21 | Method of obtaining uratoxylase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU7502117038A SU565935A1 (en) | 1975-03-21 | 1975-03-21 | Method of obtaining uratoxylase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU565935A1 true SU565935A1 (en) | 1977-07-25 |
Family
ID=20613838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU7502117038A SU565935A1 (en) | 1975-03-21 | 1975-03-21 | Method of obtaining uratoxylase |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU565935A1 (en) |
-
1975
- 1975-03-21 SU SU7502117038A patent/SU565935A1/en active
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