SU1723118A1 - Strain of micromycete monilia candida, used for purification of sewage from synthetic fatty acids - Google Patents

Abstract

The invention relates to biotechnology, in particular to the biological treatment of industrial wastewater from synthetic fatty acids, and relates to a new strain of microscopic fungi, which can be used for aerobic biological treatment of wastewater, mainly from refineries with synthetic fatty acid plants, and can also be used for wastewater treatment of dairy plants, production of synthetic fat substitutes containing fatty acids and their salts, alcohols, aldehydes and others rganicheskie compounds object of the invention - a strain of microscopic fungi that can be disposed of synthetic fatty acids to increase the degree of purification of wastewater production of synthetic fatty acids and reduce the buildup of biomass during the start-up sewage treatment plants. The invention consists in using the strain of microscopic fungi Monilia Candida VKPM F-407 on foamed polyurethane foam in biological structures, which helps in the production conditions to accelerate biomass buildup during the start-up period of aerotates and other biological purification facilities and to intensify the process of wastewater containing oily acids in concentration to 8 g / l. 3 tab. (L

Description

What about

The invention relates to biotechnology, in particular to the biological treatment of industrial wastewater from synthetic fatty acids, and relates to a new strain of microscopic fungi that can be used for aerobic biological treatment of wastewater, mainly refineries that have synthetic fatty acid (FFA) plants, and It can also be used to treat wastewaters of dairy plants, the production of synthetic fat substitutes containing fatty acids and their salts, alcohols, aldehydes and other no organic compounds

Wastewater from the production of synthetic fatty acids belongs to the category of highly concentrated waters. The total amount of impurities in the effluent of FFA in terms of bichromate oxidation capacity (COD) is 8000-13000 mg O / l, and in terms of biochemical oxygen consumption (VPK) 5500-11500 mg 02 / l. Wastewater is characterized by elevated temperature (37-40 ° C). At end

hydrogen ion concentration wastewater acidic (pH 3.0-3.5). Even after neutralization of these waters, biological treatment is impossible due to their toxicity to microorganisms of activated sludge. After neutralization, the dilution of wastewater is required by a factor of 20–25, which is practically impossible under production conditions.

In some cases, with salvo COD discharges of these waters can reach 25,000 mg O / l. In such cases, there is a massive death of organisms of activated sludge and removal of sludge from the treatment plant, after which the concentration of activated sludge is 0.3-0.5 g / l. It takes several months to restore the activated sludge concentration and the viability of microorganisms.

It is known to use Trichoderma lignorum mushrooms in order to increase the degree of purification and reduce the specific air flow rate in the wastewater treatment of the hydrolysis industry. Wastewater from the hydrolysis industry also belongs to the category of highly concentrated waters, which include fatty acids. The process of oxidation of wastewater components is carried out by fine-bubble aeration, and part of the biomass is returned to the oxidation stage.

The closest to the proposed technical essence is a method of biological treatment of wastewater containing synthetic fatty acids, which provides effective purification for combined treatment of wastewater containing FFA and petroleum products, by reducing the aeration period and air flow, increasing the content of dissolved oxygen and improve sedimentation properties of activated sludge. Wastewater containing FFA is neutralized to a pH of 6-6.5, mixed with Chlorella vulgaris algae in an amount of (3-6) -10 cells / ml, and after aging, the mixture of algae and wastewater is floated and treated with active sludge in the aeration tank along with sewage containing petroleum products. The algae culture wastewater is kept for 4-6 days at t 20-25 ° C under natural light with aeration at night, after which the algae wastewater is directed to the aeration tank. Treatment with active oil is carried out at a concentration of 2.5-3.0 g / l with an aeration time of 20-22 hours and a dissolved oxygen content in the secondary clarifier of 1-3 mg / l. During treatment, the ratio of wastewater containing SZhK and oil products, within 1: 6-1: 9. At the stage of mixing wastewater with the culture of Chiorella vulgaris algae, phosphorus is introduced as a nutrient in an amount of 7-8 mg / l.

However, Chlorella vulgaris algae are less active destructors of synthetic fatty acids than fungi. When the content of organic matter up to 8000 mg O / L in wastewater in a culture with algae takes 4-6 days to reduce the COD by 40%. When using the strain VKPM F-407 Monilia Candida for 7 hours, the organic matter content of COD is reduced by 50-70%.

Fungi are more resistant to low pH values. At pH 5.5, the death of algae was observed, while during acidification of the medium to pH 5.5, the death of the Moniiia Candida strain was not observed. For this reason, the use of a strain of the fungus is more promising for highly concentrated wastewater from the production of synthetic fatty acids.

These strains of organisms used for the treatment of wastewater containing a large amount of organic impurities, including fatty acids, are little active destructors and can be used for the treatment of wastewater with a lower content of organic substances. Their use for the treatment of highly concentrated wastewater requires a rather long residence time of these waters in the sewage treatment plant, which entails the need to increase the volume of sewage treatment plants.

The aim of the invention is the Monilia Candida microscopic strain of VKPM F-407, capable of utilizing synthetic fatty acids to increase the degree of wastewater treatment of synthetic fatty acid production and to reduce the time of biomass growth during the start-up of the sewage treatment plant.

The strain was isolated from a laboratory setup, in which natural wastewater from synthetic fatty acid production was purified by breeding.

The main indicators for the selection of the active strain-destructor were the rate of decomposition of synthetic fatty acids, a noticeable quantitative predominance of colonies on the nutrient medium compared to other microorganisms, and the frequency of occurrence with repeated seeding.

The selected strain assimilates synthetic fatty acids as a source of carbon and energy. On the environment of čapek at

reducing the concentration of sucrose from 20 g / l to 1 and 2.5 g / l and the introduction of waste water production of synthetic fatty acids containing organic impurities by COD 600 mg O / l, the strain grows well, as evidenced by the increase in biomass and a decrease in the concentration of organic COD substances. With the exclusion of sucrose from the Czapek medium and the introduction of waste water produced by FFA as the only carbon source, it is well used by the strain as a source of carbon nutrition, although the build-up of biomass occurred more slowly. The results of the development of the strain Monilia Candida on the Czapek medium at various concentrations of sucrose with the addition of waste water produced by FFA are presented in Table. one.

The results obtained indicate that the introduction of M. Candida VKPM F-407 strain in the amount of 170 mg of cells / l into the laboratory unit allows for the purification of waste water containing up to 600 mg of Ci-Ci4 synthetic fatty acids without prior adaptation. / l of bichromate oxidation oxide.

The optimal concentration of synthetic fatty acids for the development of a strain with continuous cultivation of its moisture setting is 600 mg / l; with an increase in the concentration of fatty acids to 1 g / l, the cleaning efficiency remains high - up to 80%, but sludge removal is noted. When the strain is immobilized on a synthetic foam material, the oxidative capacity of the structure increases significantly as a result of the fact that sludge removal stops and the concentration of fatty acids can be increased to 8 g / l.

The resulting strain of the microscopic fungus Monilia Candida is deposited in the All-Union Collection of Industrial Microorganisms (VKPM) under the number F-407.

Monilia Candida VKgM Shgamm No. F-407 is characterized by the following culture-morphological and physiological-biochemical traits.

Culture lno-morphological features. The colonies are woolly, white, grow well on wort: for 24 hours the diameter of the colony is 10 mm, 48 hours - 25 mm, 72 hours - 30 mm, for 5 days - 50 mm, for 8 days - 75 mm (at 25 ° C) It grows well on oats agar, on Czapek's solid medium - slow growth. On the liquid medium of čapek with the addition of waste water containing fatty acids, is growing well.

Mycelium creeping, septate, spreading inside the nutrient medium and forming dense, partially merging turfs on its surface. The hyphae of the aerial mycelium are the most potent, not very close, septate. Sporulation asexual. The beginning of the formation of conidia on the third day when grown on wort agar at 23-25 ° C. Conidia

0 ovoid, unicellular, colorless, 6-7 x 5 microns.

Physiological and biochemical signs. Aerobe. Temperature range of growth 20-30 ° C. The optimal growth temperature is 235 ° -27 ° C. Maximum growth rate at 23-27 ° C. The optimum pH is 5.5-7.0.

Relation to carbon sources. Well absorbs and grows on glucose, sucrose, lactose, fructose, grows worse on

0 mannite. Well assimilates and grows on carbon synthetic fatty acids Ci-Ci4 (waste water production of synthetic fatty acids). Uses mineral and organic nitrogen, peptone

5 uses as a source of nitrogen and carbon. On wort agar, it grows well without stimuli, on Czapek's medium, growth is slowed, significantly accelerated by the addition of biotin.

0 Well stored in a lyophilized condition. Genetic features (auxotrophy) - prototroph.

Strain activity. The synthetic fatty acid destructor Ci-Ci4 is used to purify highly concentrated wastewater containing fatty acids. The destructor retains its beneficial effect for 4.5 years.

The invention is explained in the following examples of wastewater treatment containing synthetic fatty acids, without using and using the strain Monilia Candida VKPM Ms F-407.

5 Example. The laboratory aerotank-settler is loaded with more active sludge, previously selected from urban wastewater treatment plants, at the rate of 1 g / l. For 2 days, they are aerated and a liquid mineral nutrient medium containing, mg / l: (–400, K2HP04 –100, MgS04 –50, NaCI – 50, traces of FeCb and 500 mg / l sucrose as carbon source is supplied to the aero jug. After 2 day, as a carbon source, waste water with organic content by COD 360–370 mg O / l is introduced into the specified medium. The residence time of the waste water in the aeration tank is 6 h. The monitoring of the laboratory unit operation is carried out for 30 days. aerotank

increase after 20, and then every 5 days, since earlier to increase the load is impractical because of the low cleaning efficiency, as can be seen from the table. 2

The results show that the first day of cleaning efficiency is low, which indicates the need to carry out biochemical cleaning during the preliminary adaptation of activated sludge to fatty acids. Only on the 25th day, with an increase in the COD load up to 550 mg O / L, the biochemical purification process begins to stabilize and the efficiency of reducing organic matter in CP K is 77%. An increase in the COD load on the 30th day leads to a decrease in the cleaning efficiency. As can be seen from the table, the biomass concentration decreases during the experiment, as sludge is being removed, which also indicates the unsatisfactory performance of the aerotank.

PRI mme R 2. Strain Monilia Candida VKPM F-407 grown on the environment of čapek containing 1 liter of tap water 5 g of sucrose, 100 ml of water production of synthetic fatty acids, 2 g of NaNOs, 1 g - KN2P04, 0.5 g MgS.CM, traces of FeSCU at 25 ° C in 5 days. Then the five-day suspension is added to the aerotank-settler in such a way that the biomass of the strain is in the range of 0; 6-0.8 g / l. The aeration tank is continuously fed with waste water, which is subjected to biochemical purification in the aeration tank for 6 hours. As can be seen from the table. 3, the biomass of the strain of the fungus at the beginning of the experiment is 0.63 g / l. After 6 h, the concentration of organic substances is reduced by 56% by COD, and by BODA 63%. On the second day, the concentration of organic matter in the waste water increases to 650 mg O / l. Cleaning efficiency 93% for COD and 95% for MIC. The concentration of organic substances after 20 days is up to 1150 mg O / l and further increases to 1440 mg O / l. Wastewater treatment of acids is carried out in non-sterile conditions and, in addition to M. Candida strain VKPM F-407, bacteria and protozoa develop in the biocenosis of the building, but when sowing samples from the Monilia Candida installation, it is constantly found that this organism develops well on fatty acids.

The biomass of microorganisms increases throughout the experiment, which indicates that they use the fatty acids contained in the waste water as a source of carbon nutrition.

The introduction of a fatty acid destructor into the aerotank even at a low initial biomass of 0.63 g / l allows, in a shorter time, to increase the load of organic substances contained in the waste waters of the production of synthetic fatty acids on the aerotank. From tab. 3, it can be seen that with an increase in the concentration of organic matter in the wastewater fed to the aeration tank, the sludge index increases and the biomass concentration decreases, which is associated with the removal of sludge from the aero tank. Purification efficiency at high levels of organic matter is somewhat reduced, but overall it is quite high. The disadvantage of the plant operation is the deterioration of the sedimentation properties of activated sludge and its removal from the aero tank.

When immobilization of the strain Monilia

Candida on polyurethane foam cells mycelium cells are attached to polyurethane foam, penetrate into the pores of it, and the sludge removal stops. The use of the active strain of destructor, immobilized

on the carrier (polyurethane foam), will allow in the conditions of production to accelerate the process of increasing the biomass during the launch of aerotanks and other biological treatment facilities, to which

concentrated wastewater containing fatty acids, and to intensify the process of purification of these wastewaters.

Claims (1)

Claims of the invention: Strain micromycete Monilia Candida VKPM F-407, used to purify wastewater from synthetic fatty acids. The growth of the strain Monilia Candida on the environment of čapek at various concentrations of sucrose with the addition of water production of synthetic fatty acids about Wastewater treatment of synthetic fatty acid production by activated sludge without prior adaptation Table 2