KR20020081972A - A newly isolated bacterium Serratia sp. and the removal of ammonia-nitrogen in sewage and wastewater using the isolate - Google Patents
A newly isolated bacterium Serratia sp. and the removal of ammonia-nitrogen in sewage and wastewater using the isolate Download PDFInfo
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Abstract
Description
본 발명은 신균주 세라티아 속 (KCTC 18086P) 및 이를 이용한 암모니아성 질소 제거방법에 관한 것으로서, 더욱 상세하게는 토양에서 암모니아성 질소 제거에 유효한 신균주 세라티아 속(Serratiasp.)(KCTC 18086P)을 분리하고, 이 신균주를 고농도의 암모니아성 질소와 유기물 등이 함유되어 있어 처리가 어려운 하·폐수에 적용함으로써 효과적으로 질소를 제거하는 방법에 관한 것이다.The present invention is mycobacteria strain Serratia Genus (KCTC 18086P) and a method for removing ammonia nitrogen using the same, and more particularly, the genus Cerratia genus (C.Serratiasp.
호수, 저수지 혹은 유속이 느린 하천에서 다량의 영양염류가 유입될 경우 조류(藻類) 등의 수생식물이 이상증식하며, 이들 일차생산자의 대사와 분해의 반복과정을 통하여 수중의 용존산소가 결핍되고 악취가 발생하고 저니(sediment)에는 부패물이 축적되어 수질이 악화된다. 이러한 현상을 부영양화(Eutrophication)라고 한다. 수중 질소농도의 증가는 부영양화를 일으키는 중요한 요인 중의 하나로 간주되고 있으므로, 하·폐수의 방류수내 질소의 농도감소는 수질보전을 위해 필수적인 것으로 대두되었다. 현재 대부분 하수처리장의 처리공법인 활성슬러지법은 유기물질 제거와 같은 2차 처리를 목표로 하고 있으므로 질소 제거효율이 극히 낮고, 또한 질소와 인등의 제거를 목적으로 3차(고도) 처리장을 재건설하기에는 건설비용이 너무 많이 들고, 건설기간이 길어져 방류수 수질에 문제가 생긴다. 따라서, 질소는 현재의 표준활성슬러지 처리장을 개수하여 처리하는 방법이 절실히 요구되고 있다.When large amounts of nutrients are introduced from lakes, reservoirs, or streams with slow flow rates, aquatic plants such as algae grow abnormally. Occurs and debris accumulates in the sediment, resulting in poor water quality. This phenomenon is called eutrophication. Increasing the concentration of nitrogen in the water is considered to be one of the important factors for eutrophication. Therefore, decreasing the concentration of nitrogen in the effluent of sewage and wastewater has emerged as essential for water conservation. Currently, the activated sludge method, which is a treatment method of most sewage treatment plants, aims at secondary treatment such as organic material removal, so the efficiency of nitrogen removal is extremely low, and it is difficult to reconstruct the third (high) treatment plant for the purpose of removing nitrogen and phosphorus. The cost of construction is too high, and the construction period is long, which causes problems with the quality of the discharged water. Therefore, there is an urgent need for a method of treating nitrogen by treating a standard activated sludge treatment plant.
일반적으로 암모니아성 질소 제거에 적용되는 생물학적 제거방법은 1) 활성탄을 여재로 이용한 생물학적 여과공정 및 2) 호기성 질산화균을 이용하여 암모니아성 질소를 질산성 질소로 산화시킨 후 무산소조로 반송하여 탈질균을 이용하여 질산성 질소를 환원시켜 질소가스로 탈질시키는 질산화(Nitrification)-탈질화(Denitrification) 공정이 있다. 생물학적 2차 처리 또는 질산화 과정에서 약간의 질소가 세균의 세포로 동화되므로 잉여 슬러지를 제거함으로써 질소 제거효과를 나타내게 된다. 그러나, 상기와 같은 공정에서 총질소 제거효율은 20 ∼ 50%에 불과하므로 활성슬러지공법을 일부 변형한 질소제거 공정이 사용되고 있다.Generally, the biological removal method applied to ammonia nitrogen removal is 1) biological filtration process using activated carbon as a medium, and 2) ammonia nitrogen is oxidized to nitrate nitrogen using aerobic nitrification bacteria and returned to an anaerobic tank to remove denitrification bacteria. There is a nitrification-denitrification process in which nitrate nitrogen is reduced to denitrate with nitrogen gas. Since some nitrogen is assimilated into bacterial cells during biological secondary treatment or nitrification, the nitrogen removal effect is obtained by removing excess sludge. However, since the total nitrogen removal efficiency is only 20 to 50% in the above process, a nitrogen removal process in which the activated sludge method is partially modified is used.
하·폐수 중의 질소는 유기성 질소(Org-N), 암모니아성 질소(NH3-N), 아질산성 질소(NO2-N) 및 질산성 질소(NO3-N)의 형태로 존재하는데, 미처리된 하·폐수중의 주된 형태는 유기질소와 암모니아성 질소이다. 오염초기에는 주로 암모니아성 질소로 존재하다가 오염이 회복됨에 따라 호기성 상태에서 질산성 질소로 다시 산화되는데, 이 산화과정을 질산화라고 한다. 하·폐수 중의 질소를 제거하기 위해서는 질산화와 탈질화가 모두 필요하다.Nitrogen in sewage and wastewater exists in the form of organic nitrogen (Org-N), ammonia nitrogen (NH 3 -N), nitrite nitrogen (NO 2 -N) and nitrate nitrogen (NO 3 -N). The main forms of sewage and wastewater are organic nitrogen and ammonia nitrogen. In the early stages of pollution, it is mainly ammonia nitrogen, and when the pollution is recovered, it is oxidized back to nitrate nitrogen in an aerobic state. This oxidation process is called nitrification. Both nitrification and denitrification are necessary to remove nitrogen from sewage and wastewater.
폐수 처리공정에서 생물학적 질산화는 암모니아성 질소를 아질산성 질소로 산화시키는 호기성 세균인 니트로조모나스(Nitrosomonassp.)와 아질산성 질소를 질산성 질소로 산화시키는 니트로박터(Nitrobactersp.)의 두 종에 의해 주로 이루어진다. 일반적으로 하·폐수의 질산화는 상기 미생물의 활성에 좌우된다고 알려져 있다. 따라서, 대부분의 하·폐수 처리공법이 상기 미생물의 우점화와 운전관리에 역점을 두고 있으며, 국내 출원된 특허자료에서는 상기 미생물의 활성에 중점을 둔 공정상의 처리방법에 치우쳐 있음을 알 수 있다[특허출원번호 제 1966-056853호, 제 1997-068564호].Two species of biological nitrification is nitro bakteo (Nitrobacter sp.) Oxidizing the aerobic bacteria, nitro crude Pseudomonas (Nitrosomonas sp.), And nitrite oxidizing the ammonium nitrogen to nitrite nitrogen to nitrate nitrogen in the waste water treatment process It is mainly done by In general, it is known that nitrification of sewage and wastewater depends on the activity of the microorganisms. Therefore, most sewage and wastewater treatment methods focus on the predominance and operation management of the microorganisms, and it can be seen that the patent data filed in Korea is biased in the process treatment method focused on the activity of the microorganisms [ Patent Application Nos. 1966-056853, 1997-068564].
그러나, 니트로조모나스와 니트로박터는 무기 질소원이 산화되는 산화적인산화에 의하여 생성된 에너지를 이용하여 탄소고정을 하는 화학합성독립영양생물(chemolithoautotroph)로써 비생장률이 낮고, 적정 pH 영역, 적당한 알칼리도, 높은 용존산소율을 요구한다. 또한, 폐수와 같은 복합유기성 기질이 존재할 경우 종속영양미생물의 기질이용성과 산소소모 때문에 상기 질산화미생물을 이용한 질소 제거에 상당한 어려움이 따르게 된다.However, nitrozomonas and nitrobacter are chemolithoautotrophs that fix carbon using energy generated by oxidative oxidation, in which inorganic nitrogen sources are oxidized. It requires a high dissolved oxygen rate. In addition, in the presence of complex organic substrates such as wastewater, there is considerable difficulty in nitrogen removal using the nitrifying microorganisms due to substrate availability and oxygen consumption of heterotrophic microorganisms.
또한, 고농도 암모니성 질소의 경우 니트로조모나스와 니트로박터는 각기 10 ∼ 150 ㎎/ℓ, 0.1 ∼ 2.5 ㎎/ℓ 농도의 유리 암모니아에 의해 독성 영향을 받는 것으로 알려져 있다. 이와 같은 암모니아의 독성으로 인해 질산화 미생물들이 저해를 받으면, 처리 효율이 급격히 저하되어 고농도 암모니아성 폐수를 효율적으로 처리하지 못하게 된다.In addition, in the case of high ammonia nitrogen, nitrozomonas and nitrobacter are known to be toxicly affected by free ammonia at concentrations of 10 to 150 mg / l and 0.1 to 2.5 mg / l, respectively. If the nitrification microorganisms are inhibited by the toxicity of ammonia, the treatment efficiency is drastically lowered and the high concentration of ammonia wastewater cannot be efficiently treated.
더구나, 니트로조모나스와 니트로박터는 자연계에 널리 분포하고 있으나, 이를 인위적으로 배양할 경우 낮은 생장률과 여러 가지 생장제한요인 때문에 산업적으로 이용하기 어렵다. 또한, 하·폐수 처리장에서 이들을 우점화, 안정화시키기 위한 여러 조건들을 충족시키지 못하면 결국 질소 제거효율이 낮아지게 된다. 이러한 것은, 상기 미생물이 질소만을 이용하는 독립영양생물이므로 유기원과 질소를 동시 이용하면서 낮은 C/N비에서도 생육 가능하면서 질산화 효율이 높은 종속영양생물의 필요성이 대두되고 있다.Moreover, nitrozomonas and nitrobacters are widely distributed in nature, but when artificially cultured, they are difficult to use industrially due to low growth rate and various growth limitation factors. In addition, if the sewage and wastewater treatment plants do not meet various conditions for predominantly stabilizing them, the nitrogen removal efficiency is lowered. Since these microorganisms are independent nutrients using only nitrogen, there is a need for heterotrophic organisms having high nitrification efficiency while being capable of growing at low C / N ratio while simultaneously using organic sources and nitrogen.
이에, 본 발명자들은 상기와 같은 점을 감안하여 새로운 미생물을 자연계에서 분리하고 이 미생물을 이용하여 효과적으로 고농도 암모니아성 질소를 제거하는 방법을 개발함으로써 본 발명을 완성하게 되었다.In view of the above, the present inventors have completed the present invention by developing a method for separating new microorganisms in nature and effectively removing high concentration ammonia nitrogen using the microorganisms.
따라서, 본 발명은 암모니아성 질소 제거에 유효한 신균주 세라티아 속 (KCTC 18086P) 및 이를 이용한 암모니아성 질소를 제거하는 방법을 제공하는데에그 목적이 있다.Accordingly, the present invention is effective against the strain of new strain Serratia. The purpose is to provide a genus (KCTC 18086P) and a method for removing ammonia nitrogen using the same.
도 1은 본 발명에 따른 신균주 세라티아 속 (KCTC 18086P)을 12,400 배 확대한 전자현미경 사진이다.1 is mycobacteria strain Serratia according to the present invention It is an electron microscope photograph of the genus (KCTC 18086P) magnified 12,400 times.
본 발명의 목적을 달성하기 위해 우선 암모니아를 산화시킬 수 있는 미생물을 토양에서 질산화 선택배지를 이용하여 20여종 분리한다. 이중에서 세포생장과 암모니아를 질산화시키는 활성이 우수한 미생물을 최종 선별한다.In order to achieve the object of the present invention, first, 20 kinds of microorganisms capable of oxidizing ammonia are separated from soil using nitrification medium. Among them, microorganisms with excellent activity of nitrifying cell growth and ammonia are finally selected.
분리균주를 형태학적, 생리적, 생화학적 및 구조적 분석으로 확인한 결과, 세라티아 마르세센스(Serratia marcescens)와 유사한 성질을 갖는 근연균으로 동정되었고, 이에 분리균주를 세라티아 속(Serratiasp.)(KCTC 18086P)이라 명명하였다. 본 균주를 한국생명공학연구원 유전자은행에 2001년 3월 19일에 기탁하였으며 수탁번호는 KCTC 18086P이다.As a result of the morphological, physiological, biochemical and structural analysis of the isolates, the isolates were identified as mycorrhizal bacteria with similar properties to Serratia marcescens , and the isolates were identified as Serratia sp. (KCTC). 18086P). This strain was deposited with the Korea Biotechnology Research Institute Gene Bank on March 19, 2001. The accession number is KCTC 18086P.
본 발명에 따른 신균주 세라티아 속 (KCTC 18086P)을 이용한 고농도 암모니아성 질소함유 폐수의 질소제거시 기존의 생물학적 처리시설을 이용한 질소제거가 가능하고, 신균주가 종속영양세균이므로 유기원 제거까지 동시에 일어난다. 또한, 독립영양성 질산화 미생물에 비해 균체증식 속도가 빠르고, 낮은 C/N 비에서도 생장이 활발하며, 다양한 pH 영역과 온도에서 암모니아성 질소의 제거효율이 높아 다양한 질소함유 폐수에 적용할 수 있다.Strain strain Seratia according to the present invention Nitrogen can be removed using existing biological treatment facilities when nitrogen is removed from wastewater containing high concentration ammonia nitrogen using the genus (KCTC 18086P). In addition, the cell growth rate is faster than the autotrophic nitrifying microorganism, and the growth rate is high even at low C / N ratio, and the ammonia nitrogen removal efficiency is high at various pH ranges and temperatures, and thus it can be applied to various nitrogen-containing wastewater.
특히, 암모니아성 질소의 농도가 100 ∼ 1,000 ㎎/ℓ인 폐수에 pH 6 ∼ 9, 온도 20 ∼ 40 ℃에서 암모니아성 질소의 제거효율이 높게 나타난다. 또한, 암모니아성 질소가 함유된 폐수에 신균주 세라티아 속 (KCTC 18086P)을 0.1 ∼ 2 중량%를 투입하는 것이 바람직하다. 만일 신균주를 0.1 중량% 미만으로 투입할 경우에는 낮은 질소 제거효율을 보이며, 2 중량%를 초과하면 질소 제거효율은 별다른 차이가 없으나 처리비용이 증가하게 되는 문제점이 있다.In particular, the removal efficiency of ammonia nitrogen is high in pH 6-9 and the temperature 20-40 degreeC in wastewater with ammonia nitrogen concentration of 100-1000 mg / L. In addition, it is preferable to add 0.1-2% by weight of the new strain Certiatia (KCTC 18086P) to the wastewater containing ammonia nitrogen. If the new strain is introduced in less than 0.1% by weight shows a low nitrogen removal efficiency, more than 2% by weight nitrogen removal efficiency is not much difference, but there is a problem that the treatment cost increases.
이하, 본 발명은 실시예로 더욱 상세하게 설명하겠는바, 본 발명은 다음 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited to the following Examples.
실시예 1: 신균주의 동정 및 명명Example 1 Identification and Naming of Mycobacteria
본 발명의 목적을 달성하기 위해 우선 암모니아를 산화시킬 수 있는 미생물을 토양에서 질산화 선택배지를 이용하여 20여종 분리하였다. 이중에서 세포생장과 암모니아를 질산화시키는 활성이 우수한 미생물을 최종 선별하였다.In order to achieve the object of the present invention, first, 20 kinds of microorganisms capable of oxidizing ammonia were separated from the soil using nitrification medium. Among them, microorganisms excellent in cell growth and nitrification of ammonia were finally selected.
분리균주의 형태학적 특징은 그람음성이며 간균이었다. 영양고체배지에서 콜로니는 연한 붉은색을 띠었고, 글루코오스 첨가시 생장속도는 매우 빠르며, 진한 적색을 띠었다. 분리균주의 생리 및 생화학적 특성을 조사한 결과는 다음 표 1, 표 2 및 표 3과 같다.Morphological characteristics of the isolate were Gram-negative and bacilli. In nutrient solid media, colonies were light red in color, and growth rate was very fast and dark red when glucose was added. The results of examining the physiological and biochemical characteristics of the isolates are shown in Table 1, Table 2 and Table 3 below.
상기와 같은 형태학적, 생리적, 생화학적 및 구조적 분석에서 나타난 결과로부터 본 분리균주는 세라티아 마르세센스(Serratia marcescens)와 유사한 성질을 갖는 근연균으로 동정되어 분리균주를 세라티아 속(Serratiasp.) (KCTC 18086P)으로 명명하였다. 상기 신균주를 한국생명공학연구원 유전자은행에 2001년 3월 19일에 기탁하였으며 수탁번호는 KCTC 18086P이다.From the results of the above morphological, physiological, biochemical and structural analysis, the isolated strain was identified as mycorrhizal fungus with similar properties to Serratia marcescens , and the isolated strain was identified as Serratia sp. (KCTC 18086P). The new strain was deposited on March 19, 2001 with the Korea Biotechnology Research Institute Gene Bank. The accession number is KCTC 18086P.
실시예 2: 신균주의 pH에 따른 균체증식Example 2: Cell growth according to pH of new strain
신균주 세라티아 속 (KCTC 18086P)의 pH에 따른 균체증식을 조사하여 다음 표 4에 나타내었다.Cell growth according to the pH of the new strain Ceratia (KCTC 18086P) was investigated and shown in Table 4 below.
암모니아성 질소 500 mg/ℓ가 함유된 암모니아 선택배지에 초발 pH를 각각 5, 6. 7, 8, 9로 조정하여 48시간 동안 진탕 배양시킨 결과, pH는 6 ∼ 9까지 비교적 넓은 생장분포를 보였으며 암모니아성 질소의 제거효율도 82 ∼ 91%로 비교적 높았다. 이러한 결과는 일반적으로 질산화 미생물이 약알칼리성의 생장조건을 가지는 것과 비교해 볼 때 본 발명 균주는 알카리도가 낮은 영역 혹은 질산화물에 의한 pH 저하시에도 높은 질소 제거효율을 나타낼 수 있음을 의미한다.When the ammonia selective medium containing 500 mg / ℓ of ammonia nitrogen was shaken for 48 hours by adjusting the initial pH to 5, 6. 7, 8, and 9, respectively, the pH showed a relatively wide growth distribution from 6 to 9. The removal efficiency of ammonia nitrogen was also relatively high, 82-91%. These results generally mean that nitrifying microorganisms can exhibit high nitrogen removal efficiency even when the pH of the strain is low due to the low alkalinity or nitric oxide, compared with the weak alkaline growth conditions.
실시예 3: 신균주의 최적 생장온도Example 3: Optimal growth temperature of new strain
신균주 세라티아 속 (KCTC 18086P)의 최적 생장온도를 조사한 결과는 다음표 5와 같다.The optimum growth temperature of the new strain Ceratia genus (KCTC 18086P) is shown in Table 5 below.
암모니아 선택배지에 본 발명 균주를 접종한 후, 배양온도를 20, 25, 30, 35, 40 ℃로 각각 조정하여 48시간 동안 진탕 배양하였다. 일반적으로 질산화 미생물이 32 ∼ 37 ℃ 범위에서 최적활성을 나타내는 것과 유사하게 본 발명 균주도 중온성 세균의 특징과 같이 30 ∼ 35 ℃에서 균체증식 및 질소 제거효율(89, 87%)이 높게 나타났다. 그러나, 20 ℃에서 낮은 균체증식을 나타내지만 암모니아성 질소 제거효율은 61%로 저온에서도 기존 질산화 미생물에 비해 비교적 높은 제거효율을 나타내었다.After inoculating the strain of the present invention into the ammonia selection medium, the culture temperature was adjusted to 20, 25, 30, 35, and 40 ° C, followed by shaking culture for 48 hours. In general, nitrifying microorganisms showed optimum activity in the range of 32-37 ℃, the strain of the present invention also showed high cell growth and nitrogen removal efficiency (89, 87%) at 30 ~ 35 ℃ as the characteristics of mesophilic bacteria. However, although the cell growth was low at 20 ℃, the removal efficiency of ammonia nitrogen was 61%, which was relatively higher than the existing nitrification microorganisms even at low temperatures.
실시예 4: 암모니아성 질소농도에 대한 처리효율Example 4 Treatment Efficiency with Ammonia Nitrogen Concentration
신균주 세라티아 속 (KCTC 18086P)의 암모니아성 질소농도에 대한 처리효율을 조사한 결과는 다음 표 6으로 나타내었다.The results of the treatment efficiency of the ammonia nitrogen concentration of the new strain Ceratia (KCTC 18086P) are shown in Table 6 below.
암모니아 선택배지에 암모니아성 질소농도를 50, 100, 500, 1000, 2000 mg/ℓ로 각각 조절하여 본 발명 균주를 동량 접종한 후 48시간 진탕 배양하여 균체증식과 질소 제거효율을 조사한 결과 500 mg/ℓ의 농도에서 최대 균체증식을 나타내었으며, 질소 제거효율도 90%로 가장 높았다. 질소농도 100 mg/ℓ이하에서는 낮은 균체증식에도 불구하고 암모니아성 질소 제거효율은 88 ∼ 90%로 나타났는데 이는 미생물 생육에 필요한 에너지대사원으로서 암모니아성 질소함량이 최적성장에 필요한 양보다 부족한 상태에 있는 것으로 판단된다.The ammonia nitrogen concentration was adjusted to 50, 100, 500, 1000 and 2000 mg / l in the ammonia selective medium, respectively. The maximum cell growth was shown at the concentration of ℓ and the nitrogen removal efficiency was the highest at 90%. At a nitrogen concentration below 100 mg / ℓ, despite low cell growth, the ammonia nitrogen removal efficiency was 88 to 90%, which is an energy metabolism for microbial growth. It seems to be.
실시예 5: 실제폐수에서 암모니아성 질소의 제거효율Example 5 Removal Efficiency of Ammonia Nitrogen in Actual Wastewater
신균주 세라티아 속 (KCTC 18086P)을 실제폐수에 적용시켜 암모니아성 질소의 제거효율을 조사하였다.The new strain Ceratia genus (KCTC 18086P) was applied to the actual wastewater to investigate the removal efficiency of ammonia nitrogen.
대상폐수는 부산소재 식품폐수로 암모니아성 질소가 560 mg/ℓ, 총질소는 약 600 mg/ℓ로 폐수내 질소원의 대부분이 고농도 암모니아성 질소로 이루어짐을 알 수 있었으며, 총유기탄소(TOC)는 1,250 mg/ℓ로 C/N비가 2 정도의 기존 활성오니처리법 및 질산화 미생물을 이용한 질소제거 방법으로는 적당하지 않는 폐수였다.The target wastewater was food wastewater from Busan, with 560 mg / l of ammonia nitrogen and about 600 mg / l of total nitrogen. Most of the nitrogen sources in the wastewater consisted of high concentration of ammonia nitrogen, and total organic carbon (TOC) The wastewater was not suitable for the conventional activated sludge treatment with a C / N ratio of 1250 mg / l and the nitrogen removal method using nitrifying microorganisms.
대상폐수를 실험실로 운반해 SBR(Sequencing Batch Reactor)공법을 적용시킨 파이롯트장치에서 기존 폭기조 슬러지만을 첨가한 대조구와 기존 슬러지에 본 발명에 따른 신규 미생물을 각각 0.1, 1, 2 중량%로 첨가한 실험구를 비교구로 하여 유기원과 질소 제거효율을 측정하였다. 각각의 운전조건은 4시간 폭기, 2시간 교반, 30분 침전 배출로 수리학적 체류시간은 24시간, 슬러지 체류시간은 10일로 각각 조정하였으며 2주간 연속 운전한 후 최종 3일간의 측정 평균값을 다음 표 7에 나타내었다.The experiment was performed by adding the new microorganisms according to the present invention to the control and the existing sludge in the pilot apparatus to which the wastewater was transported to the laboratory to which the SBR (Sequencing Batch Reactor) method was applied. The organic source and the nitrogen removal efficiency were measured using the sphere as a comparison group. Each operation condition was adjusted to 4 hours aeration, 2 hours agitation, 30 minutes sediment discharge, hydraulic retention time 24 hours, sludge residence time 10 days, respectively. 7 is shown.
각 결과에서 기존 슬러지만으로 운전된 대조구의 경우 유기원 제거효율은 약 90%이나 암모니아성 질소 제거효율은 약 40%, 총질소 제거효율은 43%로 기존 슬러지에서 질산화 미생물로의 유도가 완전히 진행되지 못하였다. 따라서, 암모니아성 질소의 잔존이 거의 전체질소로 남아있음을 알 수 있는 반면에, 본 발명 균주를 1% 첨가시킨 실험구의 경우 유기원 제거효율은 약 95%, 암모니아성 질소 제거효율은 약 90%, 총질소 제거효율은 약 86%로서 유기원 제거효율과 함께 질소 제거효율도 크게 향상시킨 것으로 조사되었다. 본 발명 균주의 접종량과의 관계는 슬러지내 신규 미생물의 우점화에 영향을 받는 것으로 나타났는데 0.1% 접종은 1%이상 접종에 비해 낮은 제거효율을 나타내었다.In each case, the control run with the existing sludge alone was about 90% organic removal efficiency, about 40% ammonia nitrogen removal efficiency, and 43% total nitrogen removal efficiency. I couldn't. Therefore, it can be seen that the residual of ammonia nitrogen remains almost total nitrogen, whereas in the experimental group to which 1% of the strain of the present invention was added, the organic source removal efficiency was about 95% and the ammonia nitrogen removal efficiency was about 90%. The total nitrogen removal efficiency was about 86%, and the nitrogen removal efficiency was greatly improved along with the organic source removal efficiency. The relationship with the inoculation amount of the strain of the present invention was shown to be affected by the dominance of the new microorganisms in the sludge, 0.1% inoculation showed a lower removal efficiency compared to more than 1% inoculation.
상기에 설명한 바와 같이, 본 발명에 따른 신균주 세라티아 속 (KCTC 18086P)을 이용한 고농도 암모니아성 질소함유 폐수의 질소제거시 기존의 생물학적 처리시설을 이용한 질소제거가 가능하며, 신균주가 종속영양세균이므로 유기원 제거까지 동시에 일어난다. 또한, 독립영양성 질산화 미생물에 비해 균체 증식속도가 빠르며 낮은 C/N 비에서도 성장이 활발하고, 다양한 pH 영역과 온도에서 암모니아성 질소의 제거효율이 높아 다양한 질소함유 폐수에 적용할 수 있다.As described above, when the nitrogen of the high concentration ammonia nitrogen-containing wastewater using the new strain Ceratia genus (KCTC 18086P) according to the present invention, it is possible to remove nitrogen using an existing biological treatment facility, and the new strain is heterotrophic. Therefore, the removal of organic sources takes place simultaneously. In addition, the cell growth rate is faster than that of autotrophic nitrifying microorganisms, and the growth rate is high even at low C / N ratio, and the removal efficiency of ammonia nitrogen at various pH ranges and temperatures is high, and thus it can be applied to various nitrogen-containing wastewater.
따라서, 본 발명에 따른 신균주 세라티아 속 (KCTC 18086P)은 암모니아성 질소함유폐수에 적용시 높은 성장활성에 의한 우점 가능성이 높고, 고효율의 질소제거를 유도할 수 있으며, 운전비용 및 유지비 절감을 유도할 수 있다.Therefore, the new strain Ceratia genus (KCTC 18086P) according to the present invention has a high possibility of advantages due to high growth activity when applied to ammonia nitrogen-containing wastewater, can induce high-efficiency nitrogen removal, and reduce operating and maintenance costs. Can be induced.
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| KR100850373B1 (en) * | 2007-01-19 | 2008-08-04 | 이화여자대학교 산학협력단 | Serratia sp. sy5 and method of plant growth promoting and soil purification using the same |
| KR101486120B1 (en) * | 2011-09-14 | 2015-01-26 | 고려대학교 산학협력단 | Method for removing heavy metal using siderophore produced by microbes |
| KR101661546B1 (en) | 2015-06-30 | 2016-10-04 | 경기대학교 산학협력단 | Mixed marine bacteria having excellent abillity of removing nitrogrn and phosphorus and method for removing nitrogrn and phosphorus using the same |
| CN111826326A (en) * | 2020-08-05 | 2020-10-27 | 江西农业大学 | Bacterial strain for degrading lignin in papermaking wastewater and screening method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR100850373B1 (en) * | 2007-01-19 | 2008-08-04 | 이화여자대학교 산학협력단 | Serratia sp. sy5 and method of plant growth promoting and soil purification using the same |
| KR101486120B1 (en) * | 2011-09-14 | 2015-01-26 | 고려대학교 산학협력단 | Method for removing heavy metal using siderophore produced by microbes |
| KR101661546B1 (en) | 2015-06-30 | 2016-10-04 | 경기대학교 산학협력단 | Mixed marine bacteria having excellent abillity of removing nitrogrn and phosphorus and method for removing nitrogrn and phosphorus using the same |
| CN111826326A (en) * | 2020-08-05 | 2020-10-27 | 江西农业大学 | Bacterial strain for degrading lignin in papermaking wastewater and screening method and application thereof |
| CN111826326B (en) * | 2020-08-05 | 2023-01-03 | 江西农业大学 | Bacterial strain for degrading lignin in papermaking wastewater and screening method and application thereof |
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