SU791640A1 - Method of culturing microorganisms for launching biological purification units - Google Patents

Description

The invention relates to methods for growing microorganisms active sludge during the start-up of biological treatment plants of the chemical and petrochemical industry. A known method of using mutated microflora in wastewater treatment. The method consists in the fact that for biochemical purification of water with activated sludge it is pretreated with chemical mutagens, and the treatment is carried out at a mutagen: active sludge ratio of 1: 5000 8000. The treatment of the sludge with mutagens makes it possible to increase the degree of purification of waste water from organic and inorganic impurities. . The closest to the claimed object is the method of increasing the activated sludge for the start-up period using activated sludge, which purifies any waste water for complete purification 2. Initially, diluted industrial waste water is supplied to this sludge (MIC full. 150,200 OQ mg / l) and only gradually, as a result of stable results of sludge purification and growth, does the concentration of production and effluent increase. Sludge accumulation rate h depends on the composition of the waste liquid. Growth stimulant is bacterial sludge from any production. In the summer, this takes from three weeks to two months, since it is necessary to create an appropriate complex of microorganisms capable of destroying the compounds contained in the production runoff. The main disadvantages of this method are: a long period of buildup of activated sludge during the start-up of treatment facilities; the buildup of microflora on drains containing high concentrations of organic compounds leads to the mass death of microorganisms and especially those that do not use these contaminants for their vital activity. The purpose of the invention is to reduce the time to build-up of activated sludge during the start-up of biological treatment plants and to increase the efficiency of disposal of specific contaminants. The goal is achieved by the fact that when building up microorganisms for launching biological treatment plants under aeration conditions on a nutrient medium containing sources of carbon, nitrogen and phosphorus in the presence of a bacterial growth stimulator, bacterial mutants are used as the latter, purification facilities in the amount of 0.0001-0.00002% by volume. The proposed method is carried out as follows. The industrial tank with a volume of 2000 is filled with highly concentrated industrial effluents and nutrient additives are introduced. At the same time make mutated bacterial suspension. Aeration was carried out for 4-5 days. A change in the biocenosis and a change in the chemical composition of the medium (BOC, ammonium nitrogen, phosphorus) are observed. Initially, a large number of individual bacterial cells are detected, then zoogleous forms of bacteria, amoebas, and colorless flagellates appear. On the fifth day, there is a complete change of biocenosis, protozoa appear with a well-developed peri-rotary field. The Nadilov fluid becomes transparent, the sludge drawability is good, the resulting bacterial mutants with higher enzymatic activity have the ability to more intensively reproduce bacterial cells and, accordingly, increased ability to decompose Chemicals for this production. As a result, the concentration of activated sludge is appropriate. when operating at full capacity of the sewage treatment plant, it was reached within 5 days, and the rate of destruction of contamination increased. Example 1. A tank for growing microflora with a capacity of 2000 m is filled with wastewater produced by synthetic fat substitutes with an organic concentration of 4000 MGO2. / L in COD (chemical oxygen consumption) with added nutrient salts of nitrogen and phosphorus in the ratio of BOD: N: R 100: 1.5: O, 7. At the same time, bacterial mutants were introduced in the amount of 0.0001 vol.%. The liquid in the tank is subjected to continuous aeration with 2000 air in contact conditions. The observations were made both by the formation of cotton of activated sludge and by a change in the chemical composition of the medium. After several hours of aeration, the medium became turbid. Microscopic examination revealed a large number of individual cells. A day later formed flakes of silt. After two days, co-glue clusters appeared. After five days, the claps consisted of a large number of multi-layered bakerial cells. The clusters were dense. Cotton is well separated from the purified liquid. The sludge concentration reached a design value of 2.5-2.8 g / l and, accordingly, COD decreased by 97%. The expanded specific microflora was sent to the active section of the aeration tank for further use in the treatment of wastewater from the production of synthetic fat substitutes. Example 2. Aeration of industrial wastewater with a concentration of organic pollutants of 4500, having additives of biogenic salts of nitrogen and phosphorus at a ratio of 100: 1.6: 0.8, is carried out in contact conditions. At the same time, bacterial mutants are introduced in an amount of 0.00002% by volume. Further work was carried out as in Example 1. Time for building up. Sludge is reduced by 6-7 times compared to the existing method, and the reduction in COD is 96%. The results of the buildup of activated sludge by the proposed method are presented in table. one . Table 1

150-200

Known method

4000 4500

20-60

0.3

Men- over 100

2 oh oh

2.56

At high concentrations of contamination (4000-4500). The concentration of activated sludge in 5-6 days reached 2.8-2.56 g / l, whereas in the known method, in 20-60 days, the concentration of sludge reached only 0.3 g / l.

Example 3. During industrial verification of the start-up of biological wastewater treatment plants, the experiment was used. Characteristics of the work of biological wastewater treatment plants for the production of synthetic fat substitutes at different stages of start-up.

mental line designed for processing 9.2 tons of pollution per day, or 11520 m of wastewater per day. Experimental, start-up thread includes an aeration tank with a volume of 11520 m and a secondary settling tank with a volume of 1350 m.

The results of the launch of the proposed method are given in table. 2

Table i

Claims (2)

Claim 1. A method of growing microorganisms for starting biological treatment plants under aeration conditions on a nutrient medium containing sources of carbon, nitrogen and phosphorus in the presence of a bacterial growth stimulator, characterized in that, in order to reduce the growth time, bacterial mutants are used as a growth stimulator. 2. The method according to claim 1, characterized in that the bacterial mutants contribute in an amount of 0.00010.00002% of the volume of the nutrient medium.