SU1674771A1 - Method for preparation of protein fodder product - Google Patents

Abstract

The invention relates to the field of biotechnology and relates to a method for producing a protein feed product that can be used to proteinate feeds based on starch or sugar containing feedstock, incl. waste starch production. The purpose of the invention is to increase the yield of the target product and simplify the process by using a mixed culture of microorganisms. A mixed culture containing the strain of amylolytic yeast Endomycopsis flbuligera R-574, the strain of the yeast Candida gullllermondii 21-A (VKPM No. Y-438) and the strain of pentoserostimulating lactic acid bacteria L. pentoaceticum B (b) -23 (VKPM Nfe B-1622) at a ratio of 1: 1: 20, grown on a nutrient medium containing molasses, corn flakes, animal feed or flour, at 30-32 ° C and pH 5.0-5.5. An exit to 50% from the used sugar. 1 tab.

Description

one

The invention relates to the field of biotechnology and relates to a method for producing a protein feed product that can be used to proteinate feeds based on starch or sugar containing feedstock, incl. waste starch production.

The purpose of the invention is to increase the yield of the target product and simplify the process by using a mixed culture of microorganisms.

The method is carried out as follows.

A mixed culture containing the strain of the yeast Candida gullllermondii 21-A (VKPM G Y-438), the strain of amylolytically active yeast Endomycopsis flbuligera R-574 and the strain of lactic acid bacteria lactic acid bacteria Lact. pentoaceticum B (b) -23 (VKPM Ns B-1622) in a ratio of 1: 1: 20, grown on a nutrient medium based on starch or sugar-containing raw materials (molasses, grain grains, feed, flour). The raw material is mixed with water so that the concentration of the carbon source is 1.0-2.5%, salts are added - diammonium phosphate in the amount of 2.6 kg per 10 kg of starch or superphosphate extract in the amount of 400 l and urea in the amount of 900 g per 10 kg of starch.

The process of growing microorganisms is carried out at a temperature of 30-32 ° C and pH

ABOUT

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5.0-5.5 with constant mixing and periodic aeration. The duration of the process is 89 hours. By the end of the process, the number of yeast cells in the field of vision should reach 100-120, 2000–25000 of lactic bacteria, the amount of yeast in 1 l mass of about 20 g in terms of absolutely dry matter, or about 30 g of compressed. The finished product is a mixture of medium with a yeast mass treated with a sharp peer in order to destroy the yeast cells. This operation can be carried out after mixing the yeast slurry with coarse feed to soften it. In the process of growing yeast, it is possible to add chopped straw. In this case, the yeast cells are immobilized on straw particles and their growth is activated.

The advantage of this method is the ability of the mixed culture to assimilate starch, including insoluble. The amylolytic properties of the yeast E. fibullgera in a mixed culture with lactic acid bacteria are sharply activated. Bacteria and yeasts of C. gullliermondii, using the metabolic products of the yeast E. fibuligera, eliminate the inhibition of their cells by products of their own metabolism. The resulting feed, in addition to unicellular protein, contains a complex of amylolytic enzymes that contribute to the improvement of the digestion of farm animals and is especially recommended for feeding calves whose body lacks amylase, which causes dispersion during starch loading. The presence of lactic acid bacteria prevents the development of extraneous microflora.

Example 1. The cultivation of mixed cultures of microorganisms on the medium with insoluble starch.

The yeast consortium E. fibuligera, C. gullliermondii and bacteria L pentoaceticum are cultivated on a medium with insoluble starch at a concentration of 5 g / l in a laboratory fermenter under periodic conditions at 30 ° C and a pH of 5.5 for 24 hours. In parallel, in the same the conditions of growing and the association of the yeast C. troplcalis, S. guilllermondli, T, cutaneim on the medium with glucose, since the yeast that is part of this association did not assimilate starch (prototype). The results are shown in the table.

The use of a mixed culture allows to obtain a biomass yield, 1.5 2.0 times higher than the prototype, to reduce the cultivation time

and eliminate the need for labor and energy to grow malt and carry out subsequent operations associated with the saccharification process, as well as to reduce

the cooking time of the medium is from 1-2 to 0.5 -1 h, since the mixed culture is able to assimilate insoluble starch.

PRI mme R 2. Cultivation of mixed culture on flour jams under production conditions

Grain grain is mixed with water in a ratio of 1.20 (or 1:10, followed by 2-fold dilution), and is boiled at 80-90 ° C for 2 hours. Cooled to 30-32 ° С

the mixture is added with diammonium phosphate at the rate of 2.6 kg per 20 kg of starch and sown with a mixed culture of yeast and lactic acid bacteria. The ratio of yeast and bacteria in the original culture - E. fibuligera,

S. guilliermondi, L pentoaceticum 1: 1: 20. The number of cells of each species (20-24) -10e cells / ml, bacteria (40-50) -107 cells / ml. The cultivation time was 8-9 hours. During this period, 5 g / l of starch was utilized. The final

(the content of yeast (30-40) -108 cells / ml, bacteria 1010 cells / ml.

Example 3. A mixed culture of microorganisms E. fibuligera, C. gullliermondii, L pentoaceticum grown

under the conditions of the feed shop, in a 2.5% sugar-containing medium prepared by mixing molasses with water (50 kg molasses per 1 ton of water), preheated to 70 ° C, with the addition of nutrient salts from sources of nitrogen and phosphorus (diammonium phosphate ) at 30-32 ° C, pH 5.5 for 6 hours, the ratio of yeast and bacterial cells in the 1: 1: 20 seed culture is maintained until the end of the cultivation period. Biomass yield of 1.2 g / l, i.e. about 50% of used sugar. The number of yeast cells E. fibuligera 10-106 cells / ml, C. guilllermondii 15 j.106 cells / ml, bacteria 27-U7 cells / ml.

Example 4. Mixed culture

microorganisms E. fibuligera, S. gullliermondii. L. pentoaceticum is grown under the conditions of a feed mill on a medium prepared from rice flour with water in a ratio of 1.5: 8.5. Nitrogen nutrition is brought in

in the form of urea (900 g per 1 ton of medium), phosphoric in the form of a superphosphate extract (400 ml per 10 kg of sugar). The medium contains 2.2% sugar. The biomass yield of 1.1 g / l, the number of yeast cells of E. fibuligera 8 -106 cells / hl,

C. guilllermondli 12-106 cells / hl, bacteria 20-U7 cells / hl.

Example 5. A mixed culture of microorganisms MOB E. fibuligera, C. guilliermondil was grown on a yeast plant installation under conditions of a feed mill. L. pentoacetlcum 8 (6) -23 on the medium prepared as a result of cooking 70% crushed wheat and 30% bran. The original medium contains 2.5-3.5% sugar, pH 5.0-5.5. Nitrogen and phosphate nutrition are introduced in the form of diammonium phosphate at the rate of 2.6 kg per 10 kg of sugar. After 6-8 hours of cultivation, the biomass yield is 1.25-1.75 g / l. The number of cells of the yeast E. fibuligera 12-106 cells / hl, C guilliermondli 18-106 cells / hl, bacteria 25--7 cells / hl.

The final product is a mixture of microbial biomass and medium. When using grain grain or another complex substrate, it is a mixture of biomass and the remaining undigested starch-containing raw material; when using pure sugar, it is actually a mixture of biomass and culture fluid; in the case of molasses, of which 50% is represented by free sugar, a non-utilizable part remains in the culture fluid. Since the way is intended

Claims (1)

for sale in feed houses directly in households, the product is used as it is received and does not require drying. Invention Formula The method of obtaining protein feed product by growing a mixed culture, including the strain of the yeast Candida guilllemondi 21-A (VKPM No. Y-438), on a nutrient medium containing saccharized starch-containing raw materials as a carbon source, as well as sources of nitrogen and phosphorus, characterized in that yield of the target product and simplify the process, use a mixed culture containing the strain of amylolytically active yeast Endomycopsls fibuligera R-574, the strain of the yeast Candida guilliermondil 21-A (VKPM M U-438) and the strain of lactic acid bacterium Lact, pentoacetlcum B (B) -23 (VKPM N: B-1622) in the ratio of 1: 1; 20, and the process is carried out at a temperature of 30-34 ° C and pH 5.0-5.5.