SU1659469A1 - Chamber for cultivation and investigation of microorganisms in microscope field of vision - Google Patents

Abstract

The invention relates to technical microbiology, to chambers for the cultivation and examination of microorganisms in the field of view of a microscope. The purpose of the invention is to increase the reliability of the camera and ensure constant cultivation conditions. The chamber contains two glass bottom 1 and top 2 plates and a wall 3 placed between them in the form of a hollow cylinder of agar gel. The cavity 4 inside the wall 3 serves to accommodate the gel block 5 with microorganisms and the nutrient medium. The top plate 2 is placed on the wall 3 with a gap 6 for gas exchange, introduction and removal of the nutrient medium. The chamber provides, along with the reliability of the design, optimal conditions for the growth and development of aerobic microorganisms, which in turn affects the accuracy of the study. 2 Il. yo

Description

The invention relates to technical microbiology, to chambers for the cultivation of microorganisms and their study under the control of a microscope.

The purpose of the invention is to increase the reliability of the camera and ensure constant cultivation conditions.

FIG. 1 shows a camera; in fig. 2 shows section A-A in FIG. one.

The chamber for the cultivation and study of microorganisms contains two horizontal glass one above the other bottom 1 and top 2 plates and a vertical closed wall 3 placed between them with the formation inside it of a cavity 4 for a gel block 5 with microorganisms and a nutrient medium. The wall 3 of the chamber is made in the form of a hollow cylinder of agar gel, while the upper plate 2 is located on

wall 3 with the formation of a gap 6 between its edge and wall 3, which serves for the introduction and removal of the nutrient medium and gas exchange. The chamber can be placed in a Petri dish 7.

Cultivation and research in the chamber is carried out as follows.

Before starting work, wall 3 is fabricated and a chamber is assembled. From a gel plate of a given thickness of the box, pressures of pressure of various diameters

The wall 3 is stamped in the form of a cylinder with an outer diameter not exceeding the dimensions of the glass plate 1. The cylinder is placed on the bottom glass plate 1. In the center of it is placed a gel block 5 stamped from the same gel plate with a diameter smaller than the internal diameter of the cylinder-wall 3 . The free surface of the gel block 5 seeded

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the test microorganism. Then, the upper glass plate 2 is placed on the wall 3 and the block 5 so that between it and the inner space of the wall 3 on one side there is a gap 6. Through this gap the space between the glass plates 1 and 2, the wall 3 and the gel block 5 liquid nutrient medium optimal composition for the microorganism under study. The resulting chamber is incubated under optimal conditions for the microorganism under investigation. In the process of incubation, if necessary, liquid nutrient medium is sucked off through the gap b and replaced with fresh or other composition liquid medium. The observation of the growth and development of the microorganism under study in the chamber is carried out using microscopy. The gas exchange of the microorganisms with the external environment is also carried out through the gap 6.

The construction of the chamber wall 3 in the form of an agar gel hollow cylinder, which is a regular geometric figure with parallel ends, makes it possible to form a cavity 4 for cultivating the correct shape, and the good adhesive properties of the gel ensure stable contact and tightness with the glass plates 1 and 2, which increases the reliability of the chamber design over the time of cultivation and study of microorganisms.

Using only agar gel and camera body materials

glass, as well as the presence of a constant gap 6 for gas exchange and medium exchange and the constancy of the geometry of the internal cavity 4 in the chamber, make it possible to maintain constant cultivation conditions and reproduce them when re-examining microorganisms or using another chamber of the same design or parallel cultivation in several such cameras.

Thus, along with the reliability of the design, the chamber provides optimal conditions for the growth and development of aerobic microorganisms, which in turn affects the accuracy of their visual studies under a microscope.

Formula of the Invention A chamber for cultivating and examining microorganisms comprising two horizontally placed glass plates one above the other and a vertical closed wall interposed between them to form inside it a cavity for a gel block with microorganisms and nutrient medium, chamber reliability and ensuring constant culture conditions; the chamber wall is made in the form of an agar gel cylinder, while the upper plate is located on the wall to form m gap between its edge and the wall serving for insertion and extraction of the culture medium and gas exchange.

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Claims (1)

Claim A chamber for culturing and researching microorganisms, comprising two glass plates horizontally arranged one above the other and a vertical closed wall placed between them to form a cavity 25 for a gel block with microorganisms and a nutrient medium inside it, characterized in that, in order to increase the reliability of the chamber and to ensure constant cultivation conditions, the chamber wall is made 30 in the form of a cylinder of agar gel, while the upper plate * is located on the wall with the formation of a gap between e e edge and wall, which serves for the introduction and extraction of a nutrient medium and gas exchange. 0U9.1 FIG. 2