SU1655441A1 - Method of production lysine-containing feed additive - Google Patents

Abstract

The invention relates to biotechnology. and relates to the preparation of lysine-containing feed additives, which are widely used in the invention, relates to agriculture and can be used to obtain lysine-containing feed additives. The aim of the invention is to improve the quality of the target product. The essence of the method is as follows. Culture - producing lysine grown in a nutrient medium, the culture fluid is partially evaporated in a vacuum evaporation unit. The concentrated culture liquid is mixed with a filler and this process is carried out when the suspension is circulated and exposed to an acoustic field with a frequency of 15-20 kHz and a power of 0.6-1.6 W / cm2. In this case, the suspension can be exposed to the acoustic field at the same time throughout the whole volume or can flow through one or several zones of acoustic impact. agricultural production. The aim of the invention is to improve the quality of the target product. In a method for producing a lysine-containing feed additive by growing a producer's culture, concentrating the culture liquid with a filler, granulating the resulting mass and drying the granules in a fluidized bed, mixing the concentrate of the culture liquid with a filler is carried out by circulating the suspension with simultaneous exposure to it with an acoustic field frequency 15-20 kHz and a power of 0.6-1.6 V / cm2 The invention allows to increase the lysine content in the target product by 2.5-3.5% and reduce the consumption of filler by 30% After completion of the mixing process, the concentrate of the culture fluid and the mass of filler obrazovavsha with granulated and the granules are dried in a fluidized bed according to known techniques. Example 1 The culture of Brevilacterum E-53 lysine producer is grown in an apparatus with a volume of 100 m15 on medium containing, wt.%; molasses 27, acid hydrolyzate of fodder yeast 6, salt solution with ammonium chloride concentration 21; chalk 0.5; antifoam 0.13; water technical rest. After 72 hours of cultivation, 61.6 m of culture liquid was obtained. The culture fluid was partially concentrated in a vacuum evaporator to a dry matter content of 50%. The weight of one stripped off culture liquid was 17920 kg. The concentrate of the culture liquid in portions of up to 10 m was transferred to a mixer with a circulation w B ta NM w OK SL SL fc

Description

current total volume of 16 m, and then there was introduced in 1018 kg of wheat bran with a moisture content of 12% as a filler. The suspension was forced to circulate through the acoustic zone at a rate of 25 l / s. The sweep in the acoustic field with a frequency of 17.5 kHz. Dust was carried out in a rotary unit with modulation of the flow. The exposure time to the acoustic field was 0.12 s. and The total circulation time of the suspension was 1.11 hours. The mass formed was granulated in a fluidized bed dryer-granulator with a bed temperature of 75-80 ° C, resulting in 18200 kg of granulated preparation with a moisture content of 5%. A total of 10,182 kg of bran was consumed. The lysine content in the finished product was 13% (2248 kg).

The specific consumption of filler in the implementation of the method was 0.56 kg / kg. The final product showed compliance with all specifications of the Feed Concentrate Lysine.

PRI mme R 2. The process for preparing a lysine-containing feed additive was carried out as in Example 1, but the frequency of the acoustic effect was 15 kHz. It was received 18290 kg of the finished product with a lysine content of 12.5%. The filler consumption was 0.61 kg per 1 kg of product.

EXAMPLE 3 The process of preparing a lysine-containing feed additive was carried out as in Example 1, but the frequency of the acoustic effect was 20 kHz. 18150 kg of the finished product with a lysine content of 13.5% was obtained. Consumption of filler was 0.52 kg per 1 kg of product.

Example 4: The preparation of the lysine-containing feed additive was carried out as in Example 1, but the acoustic power was 1.1 W2. Received 18210 kg of the finished product with a lysine content of 13.2%. Consumption of filler was 0.55 kg per 1 kg of the Product.

EXAMPLE 5 The process of preparing a feed-containing feed additive was carried out as in Example 1, but the acoustic power was 0.6 W / cm2. Received 18310 kg of the finished product with a lysine content of 12.3%.

The filler consumption was 0.63 kg per 1 kg of product.

Example 6: The preparation of the lysine-containing feed additive was carried out as in Example 1, but the acoustic power was 1.6 W / cm. Received 18080 kg of the finished product with a lysine content of 13.8%. Consumption of filler was 0.51 kg per 1 kg of product.

Example. Cooking process

5 of the lysine-containing feed additive was carried out as in Example 1, but the acoustic power was 0.3 W / cm 2. Received 18730 kg of the finished product with a lysine content of 11.4%.

0 Consumption of filler was 0.73 kg per 1 kg of product.

Example The process of preparing the lysine-containing feed additive was carried out as in Example 1, but the power of the acoustic effect was 2.0 W / cm. Received 18190 kg of the finished product with a lysine content of 13.4%. Consumption of filler was 0.54 kg per 1 kg of product. The proposed method allows to reduce the consumption of filler by 142.5 kg (30%).

Claims (1)

Invention Formula The method of obtaining lysine-containing 5 feed additives by growing the producer culture, concentrating the culture liquid, mixing the culture liquid concentrate with the filler, granulating the resulting 0 mass and drying the resulting granules in the fluidized bed, characterized in that, in order to improve the quality of the target product by increasing the Here, lysine, the mixing of the concentrate 5 of the culture liquid with the filler, is carried out by circulating the suspension and exposing it to an acoustic field This is 15–20 kHz and a power of 0.6–1.6 W / cm2.