SU1613101A1 - Method of obtaining feed - Google Patents

Abstract

The invention relates to agriculture, in particular to methods of producing feed for livestock. The aim of the invention is to improve the quality of the target product. In the method of producing food, including growing yeast on a nutrient medium containing a carbon source, thermolysis of the biomass obtained and adding a carbon source to it, the carbon source is divided into three equal parts, the first of which is saccharified until complete hydrolysis and added to the nutrient medium, and the other two are saccharified to obtain ratios of starch, dextrin and monosaccharides in the hydrolyzate, respectively, 4: 3: 3 and are added to the biomass grown before its thermolysis. The invention allows to improve the quality of feed due to enrichment with digestible protein, essential amino acids, vitamins and easily digestible carbohydrates, which provides an increase in livestock productivity by 10 - 15%. 1 tab.

Description

. The invention relates to agriculture, in particular to methods of producing feed for livestock.

The purpose of the invention is to improve the quality of the target product by increasing the digestibility and biological value of the feed.

Example 1. 30 tons of off-grade grain (third and fourth stages of defectiveness, i.e., unsuitable for food use and for feed purposes) with a putrid-musty odor with a completely changed casing brought to brown-black or black; frosted or dry wind grain with a moisture content of 17 5% starchiness 52% is subjected to water-heat treatment.

The crushed grain is fed to the mixer, where it is mixed with water preheated to 4 ° C. 2.5 liters of water are consumed per 1 kg of grain. Kneading is heated to a liquefaction and an obyamylase is set at the rate of 0.5 u / g of starch in the form of your culture. subtilis and incubated for 1 h. At this temperature, the breakdown of proteins to amino acids by the action of the proteases of the raw material. Then the mixture is heated to Cu and kept under stirring for b hours. At the same time, dissolution and partial hydrolysis of starch under the action of enzymes contained in the raw material occurs. Then, heat treatment is carried out in teCi

1 hour at 95 ° C. The pulverized mass is cooled until it is divided into three equal flows, each of which is boiled with you enzyme preparations. -: ubtilis and Asp. awamori, Mild cooking mode ensures maximum safety of the feedstock nutrients,

The first fully hydrolyzed stream, at a rate of 33.3 m, is mixed with 100 m of water to a baseline carbohydrate concentration of 4.0% and sent as a nutrient medium to three fermentors with a useful volume of 40 m.

The influx of medium into each fermenter enters differentially: first 20%, after 4 hours of cultivation 20%, then after 4 hours 30%, the last portion of water after 4 hours 20% of the useful volume. At the beginning of the cultivation process, Candida tropicalis seed yeast, as well as carbamide in an amount of 0.15 kg, diammonium phosphate 0.07 kg, and potassium chloride 0.03 kg are introduced into the fermenters. Salts are pre-dissolved in water to obtain a 20% solution.

In fermenters, biomass is grown under aerobic conditions with air supplied to the medium 60 per hour. The process is carried out within 14 hours before accumulation in the culture fluid of yeast cells in the amount of 900 ppm. The finished suspension is sent to the demulsifier to break the foam, then to the mixer-thermolizer where simultaneously a stream of xapeHHoi o wort is supplied in the amount of 66.6 m (the ratio of starch: dextrin: monosaccharides is 4: 3: 3). The finished feed product is subjected to thermolysis at 85 ° C for 1 hour and sent to the consumer. Characteristics of the finished product are presented in the table.

PRI mme R 2. 100 tons of substandard potatoes containing a large number of greened tubers with growths, dead tubers, tubers with mechanical damage, damaged by agricultural pests, diseases affected (oospores or scab, dry rot, late blight and rust), frozen potatoes with a moisture content of 16% are subjected to water-heat treatment. Crushed potatoes are continuously fed into the mixer, they dry up to 45 ° C, Oi-amylase is set to liquefy you. subtilis

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at the rate of 0.5 units / g of starch, the mixture is kept for 20 minutes. Stirring is then carried out for 1 hour at. The pulverized mass is cooled to 58 ° C, divided into two streams at a ratio of 1: 2, each of which is saccharified by you enzyme preparations. subtilis and asp. awamori, get wort in the amount of 34 m and 66 m, respectively, with a total solids content of 18%, of which. 15.5% soluble. The wort is divided into three equal flows.

The first fully hydrolyzed stream in the amount of 33.3 m is mixed with 50 m of water and 50 m of yeast fugate to the initial carbohydrate concentration of 3.9% and sent to three fermenters with a useful volume of 40 m.

The cultivation of yeast are in example 1.

The prepared suspension is sent to a demulsifier to break up the foam, then large inclusions are separated into a vibrating screen, 1 large particles are separated, and the mass passing through the sieve is subjected to separation. The resulting separated centrate in the amount of 50 m is returned to dilute the wort before fermentation in place of part of the water. Cx inflamed biomass is directed into the mixer-thermalizer, which simultaneously feeds the pellet from the vibrating screen and the second stream of saccharified wort in the amount of 66.6 m (the ratio of starch: dextrin: monosaccharides is 4: 3: 3).

The finished feed product is subjected to thermolysis at 85 ° C for 1 hour and sent to the consumer. Characteristics of the finished product are shown in the table.

Example 3. Grain wastes - wheat bran with a moisture content of 14.8%, starchiness of 45% is prepared according to Example 1. The pulverized mass is divided into three equal flows, saccharified. culture liquid enzyme preparations you. subtilis and asp. awamori

The first fully hydrolyzed stream is mixed with 100 m of water to an initial carbohydrate concentration of 4.0% and sent as a nutrient medium to three fermenters with a useful volume of 40 m.

Cultivation of yeast and the preparation of feedstuffs were carried out according to example 1.

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The table shows the characteristics of the finished product.

The invention improves the quality of feed due to enrichment with digestible protein, essential aMii-acids, vitamins obtained by microbiological synthesis, and easily digestible carbohydrates, which provides an increase in livestock productivity by 10-15%.

Claims (1)

Invention Formula The method of obtaining food, including the cultivation of yeast on nutrient sixteen a medium containing a carbon source, thermolysis of the biomass obtained and the addition of a carbon source to it, characterized in that, in order to improve the quality of the target product, the carbon source is divided into three equal parts, the first of which is saccharified to full hydrolysis and the other medium is saccharified to a ratio of starch:: dextrin: monosaccharides equal to, respectively 4: 3: 3, in the hydrolyzate and added to grown biomass before its thermolysis. Chemical composition: moisture,% dry matter, g / l content: a) ash,% abs. dry substance b) crude protein,) true protein,) ammonium nitrogen,) nucleic acids, eleven e) LIPID-,) carbohydrates,) vitamins, m1 / kg: S ( AT BE RR mineral acid composition,% and abs. dry matter: tryptophan lysine histidine asparic acid ar1 inin threonine,. serine aggregate) glutamic acid proline 1 LICIN alanine cystine valine methionine isoleucine leucine. tyrosine phenylalanine Content of ash elements,% Abs. dry matter: Editor A.Dolinich Compiled by E.Maistrenko Tehred L. Serdyukova Corrector A. Osaulenko Order 3847 Circulation 543 VNIIPI State Committee for Inventions and Discoveries at the State Committee on Science and Technology of the USSR 113035, Moscow, Zh-35, Raushsk nab. 4/5 Table continuation 0.4 3.5 2.8 1.3 3.2 0.38 3.55 2.77 1.31 3.21 Subscription