JPS62104553A - Solid composition for animal nutrition - Google Patents

Abstract

PURPOSE:The titled inexpensive composition having low water vapor absorption, high lysine content, improved compatibility with feed and shelf stability, obtained by cultivating a bacterium capable of producing lysine in a specific culture medium and concentrating and drying the culture mixture. CONSTITUTION:A bacterium such as Brevibacterium lactofermentum FERM-P 2654 mold, etc., capable of producing lysine is cultivated in a lysine producing medium having a composition shown by the formula I using NH4Cl instead of (NH4)2SO4 at about 31.5 deg.C while adjusting the medium to about 7pH with ammonia water, etc. and a saccharide solution and NH4Cl continuously fed to the medium from the middle of the culture to give a fermented solution containing 50-70wt% (based on total solid material in the fermented solution) lysine hydrochloride. Then, the fermented solution is optionally pretreated by centrifugal separation, etc., to remove a bacterium mold, dyestuff, etc., concentrated at 20-80 deg.C in a short retention time until a solid content reaches 30-65wt% and dried by filter mat spray dry method, etc., in such a way that the concentrate will not undergo thermal denaturation to give the titled granular composition having improved flow properties and low cohesive force.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of the Invention The present invention relates to solid compositions for animal nutrition containing lysine hydrochloride.

BACKGROUND OF THE INVENTION Lysine intended for animal nutrition is mixed with various feeds such as cereals, the composition of which is very poorly defined. It is considered that it is not necessary to use isolated and purified lysine under these conditions. Therefore, attempts have been made to directly solidify the lysine culture solution.

For example, Japanese Patent Publication No. 49-31794 discloses a method for producing a solid composition by concentrating a lysine culture solution and directly drying it with a spray dryer, drum dryer, or the like. However, with this method, it is difficult to produce and recover dry solids if the culture solution contains a large amount of sugar or organic acids, and the produced composition is sticky and highly hygroscopic. This makes it difficult to handle.

USSR Patent No. 480397 and French Patent Publication 22
No. 17347, No. 2464032, etc., in order to improve the hygroscopicity of the solid composition, a moisture-proofing substance is added to the lysine culture solution and dried with a spray dryer, drum dryer, etc., to obtain a solid composition containing 10 to 35% lysine. It is proposed to manufacture something. However, these compositions have a relatively low lysine content, resulting in a high additive content.

In addition, French Patent Publication No. 2541867 states that the ff1ffi ratio of fermentable substances/total solids of carbon source is 80%.
Solid compositions with improved physical properties are disclosed that are made larger and azeotropically distilled under reduced pressure to a lysine content of 35-48% with the addition of suitable drying aids. However, these compositions still have problems such as low lysine content, high production costs, and the tendency for the products to become fine powders.

Problems to be Solved by the Invention Development of a solid composition for animal nutrition with a high lysine content that is less hygroscopic, has good mixability with feed, is easy to handle, and can be produced at reduced cost. is requested.

Means for Solving the Problems As a result of intensive research, the present inventor found that Lys・1/2 H2 is the cause of high hygroscopicity when lysine content is high.
It has been found that SO4 itself is extremely hygroscopic, and that by converting lysine into Lys-)-1CI, the hygroscopicity can be reduced even if the lysine content is high.

That is, the present invention provides a solid product for animal nutrition obtained from a culture medium of a lysine-producing microorganism, characterized in that lysine is present in the form of hydrochloride, and contains 50 to 70% as lysine hydrochloride. It is a stable composition.

In preparing the culture solution used in the present invention, microorganisms that can be used include Brevibacterium lactofermentum (3 revibacterium lactofermentum).
mentun+), FERM-P2654. FERM
- Using a microorganism capable of producing lysine such as P4O10, it may be utilized according to the lysine fermentation method described in Japanese Patent Publications Nos. 53-43591 and 51-34477.

However, ammonium chloride was used instead of ammonium sulfate in the production medium, and as lysine was produced, Ll
/5-HCI is formed.

The lysine content based on the total solids in the fermentation liquid thus obtained is 50 to 70% as lysine hydrochloride. In addition, this fermentation liquid contains microbial cells, some pigments, and the like.

These may be pre-treated to be removed by centrifugation, filtration, activated carbon, etc. In this case, the amino acid content per total solid matter in the feed composition is improved.

When concentrating the fermentation liquid, in order to prevent thermal denaturation of lysine, it is effective to use concentration equipment with a low temperature and short residence time as much as possible, and the liquid temperature is preferably within the range of 20 to 80°C. Concentration is carried out under such conditions until the solid content is approximately 30 to 65%.

When solidifying this concentrate, for example, filter mat spray drying (Fi
It is preferable to adopt the ltermat 3play Qry) method. The solid obtained in this way is
It has good fluidity and low agglomeration, so it has good mixability.
The result is granules that are very easy to remove.

This lysine hydrochloride composition is stable during storage and has nutritional effects comparable to purified lysine.

Hereinafter, the present invention will be explained in detail with reference to Examples.

Example Preparation of lysine fermentation liquid: Lysine production medium 8001 having the composition shown in Table 1 below was added to 2k
1) Place in an incubator, sterilize and aerate to 1/2 VVm.

Under the conditions of stirring at 25 rpm and temperature at 31.5°C, adjusting the pH to 7 with aqueous ammonia, Japanese Patent Publication No. 53-4359
Brevibacterium lactofermentum FERM-2654 described in Publication No. 1 was cultured. Sugar solution and ammonium chloride were continuously supplied during the culture to obtain 10 g of lysine hydrochloride/1 kl of the old fermentation liquid.

Table 1 Lysine fermentation liquid medium composition Concentration and solidification of culture liquid: The culture liquid thus obtained was concentrated to 40% moisture using a calandria type vacuum concentrator.
, IA condensate liquid 2501 was obtained.

This concentrated solution was dried to a moisture content of 2% using a DEC filter mat spray dryer. In this process, J5 has a concentrated liquid supply of 13,501 ft/hr and hot air of 4 degrees Celsius and 120 ft.
By heating to 10°C, soft granules with an average dry particle size of 450μ were obtained.

The evaluation results of thermal denaturation and hygroscopicity of the lysine hydrochloride granules thus obtained are shown in Table 2 and the drawings, respectively.

As a result, it can be seen that the filter mat/spray dryer-dried product of lysine hydrochloride culture solution has low hygroscopicity and little thermal denaturation.

The granules also had very good fluidity and mixability, and had sufficient physical properties when added to feed.

Table 2 Heat denaturation evaluation Ai: 4 after diluting 5 g of solid content to 100 cc with water
As is clear from the above, the present invention provides a solid composition for animal nutrition that has low hygroscopicity, good mixability with feed, is easy to handle, and has a high lysine content. can do.

[Brief explanation of drawings]

The drawing shows the moisture absorption characteristics of a solid composition according to an example of the present invention and a conventional product at 40° C. and a relative humidity of 70%. I: Lys・:L/2H! 2 S O4 pure product port: Ly
s・1/2 H2S○4 broth dried product C: Lys-HC
I broth dried product 2: Lys-HCI pure product patent applicant Ajinomoto Co., Inc. 4-Open

Claims (1)

[Claims] It is obtained from a culture medium of a lysine-producing microorganism, in which lysine exists in the form of hydrochloride, and 50 to 70% as lysine hydrochloride.
A solid stable composition for animal nutrition, characterized in that it contains %.