SU1507787A1 - Method of producing protein food additive - Google Patents

Abstract

FIELD OF THE INVENTION The invention relates to biotechnology and relates to the production of feed additives that can be used for the production of protein enriched feed. The aim of the invention is to improve the quality of the product by enriching it with protein and other biologically active compounds, as well as accelerating the production process. To do this, a solid nutrient medium consisting of 42-45% wheat bran and 52-55% vegetable waste (grape, fruit and vegetable extracts, vine pruning, tea beverage production waste, essential oil or starch production) is moistened to 52- 54% urea solution so that the concentration is 3.0-3.5%. Then the urea solution is neutralized with phosphoric acid so that the pH of the nutrient medium after wetting and sterilization is in the range of 4.8-5.5. Then the medium is sterilized, cooled and seeded with the seed culture of the yeast-like fungus ENDOMYCOPSIS FIBULIGERA C-4 in the amount of 5-10% of the air-dry medium. The seed is obtained by deep cultivation of the producer on an optimized medium of the following composition, wt.%: Wheat bran 2.5-3.7

vegetable waste 2.0-2.5

malt sprout extract 7-10

/ NH _4/2 HPO ₄ 0.05-0.15

K ₂ HPO ₄ 0.10-0.15, and the rest is water. Solid phase fermentation is carried out at 33-35 ° C, air humidity of 98-100% in a thin stationary substrate layer with a height of 35-40 mm with aeration on average, depending on the stage of culture development, 1.5-30-10 m ³ / h ^. kgASV or in a thick layer 200-220 mm high with volumetric aeration of the medium, which in the exponential phase is 1.0-1.2 m ³ / h ^. kg ACB. The duration of the fermentation process is 20-24 hours. The finished culture is dried at 105 ° C. The content of crude protein in the final product is 28-40%, the true protein is 22-34% with a protein content in the original nutrient medium of 9-21%. 1 hp ff, 2 tab.

Description

The invention relates to biotechnology and relates to the production of feed dabs and bavok that can be used during the production of belgat-rich feed.

The purpose of the invention is to increase the quality of the product by enriching iero with protein and others. biologically active compounds, as well as acceleration of the production process,

In the process of obtaining a feed additive, the nutrient medium containing in | quality of carbon and nitrogen nutrition) 5 wheat bran and plant waste, then sowing and cultivation of the endomycopesis fibuligera C-4 strain on solid phase medium, followed by drying of the 20 culture. At the same time, grape pomace or postural trimming, or other waste is used as waste from the plant, and urea is additionally introduced into the nutrient medium 25, followed by neutralization with phosphoric acid to a pH of 4.8-5.5 and cultivation at 33- 35 ° C and humidity of 52-54% for 20-24 h in the following ratio of components, 30 wt.%:

Wheat Bran42-45

Grape squeeze or

pruning vines, or other

waste52-55

Urea 3.0-355

The finished material is grown in a nutrient medium of the following composition, wt.%:

Wheat bran. 2.5-3.7 40

Grape Marc

or pruning a vine, or

other waste2.0-2.5

Malt Extract

sprouts (1: 5), 7.0-10ur 5

(W) HP040.05-0.15

, 10-0, T5

Vodado 1 l.

Fruit and vegetable extracts, wastes from the production of tea drinks, wastes of starch and ether-oil production are used as vegetable waste.

The proposed method provides a short technological cycle with a duration of 20–24 h, which allows the process to be conducted in semi-septic conditions without preliminary sterilization of the blown air, 50

0

s

0

five

g

0

significantly broadens the raw material base for the production of feed additives, allows to obtain feed additives with a crude protein content of 28-40% and true protein .22-34% with a protein content of 9-21% in the original nutrient medium, while also enriching the medium ( the content of reducing substances increases by 3–3.5 times, the content of fiber is reduced by 20% 5, which can compensate to some extent for small expenses for sterilization (1 atm, 1 h), neutralizes it from extraneous microflora ogradnyh marc contamination was 25-270 million

microorganism cells 1.g, of which bacteria, mold and lumps were 65, 25 and 10%, respectively: the infection of wheat bran was 10-80 million cells / g). In addition to sterilization of the nutrient medium, the safety of the obtained product is a short producer lagfase (4-6 h) 5 preventing the development of extraneous microflora during cultivation (toxicological studies have established the harmlessness of the resulting finished product).

Thus, according to the method, harmless toxin-free products are obtained.

The product poured on a medium containing extracted grape marc, wheat bran and urea, is not toxic as a source of nitrous litany. Its biological value, the efficiency ratios b.elka.

Table 1 shows the amino acid composition of the protein of the finished product in comparison with the amino acid composition of the protein of the raw material.

Amino acid composition of the protein of the finished product (g / 100 g of protein) and its comparison with the standard FAS for essential amino acids are given in table 2.

From Table 1 it can be seen that the amino acid composition of the protein of the final product is significantly improved, both quantitatively and qualitatively, compared to the protein of the raw material, and the content of essential amino acids (Table 2), with the exception of cystine (tryptophan

not defined) exceeds the FAO standard.

The content of nucleic acids in the product is 1.7-2.3%.

The product contains a large set of hydrolytic enzymes with activity, units / g: amylolytic activity (AC) 4; saccharifying (OS) 38; pectolytic (PX) 0.6; proteolytic (PS) 1,2; glucoamylase (GLS) 17; cellulase (C) on filter paper 16; xylanazne (CS) 14; cellobiasin 1.2.

As a result of hydrolytic enzymes, the producer utilizes 70-75% starch, 53-58% pectin ,. 44-48% hemicellulose and 12-15% cellulose contained in the substrate; 87-98% sucrose reducing is absorbed.

In preparing the nutrient medium, extracted (washed) grape marc is used (various batches of extracted grape marc from various industrial grape varieties are used). Example 1. Solid nutrient medium consisting of a mixture of extracted grape marc (54%) and wheat bran (42 %) moistened to 52-54% urea solution so that the concentration of extra nitrogen nitrogen was 2% of the air-dry nutrient medium. Before moistening, phosphoric acid is introduced into the urea solution so that the pH of the nutrient medium after moistening and sterilization is in the range of 4.8-5.5 (per 100 ml of 4% aqueous solution

urea is administered 1.3-1.5 ml).

 The humidified environment is sterilized at

1.0 atm for 1 h, cooled to i 35 C and sown with seed culture End.fibuligera C-4 in the amount of 5-10% of the air-dry environment.

Seed material is obtained by the deep method of cultivation of the producer on the medium of the following composition,% by volume of the nutrient medium: wheat bran 3.7; grape marc 2.0; malt sprout extract 7.0; (NH). ,, 05; K, NR040,1; the rest of the kind. The seed is grown at 33-35 ° С, pH of the medium / dy is 5.0-6.0, for 14-16 h on a rocking chair or 8-10 h in the fermenter with aeration of the medium 0.8-1.0. Air volume / volume of medium.

Solid-phase φ, rementation is carried out at 33-35 С m from EH

15

- 2S

30 077876

spirit of 98-100%. The air supplied to the aeration does not require prior sterilization. The culture is grown in a thin stationary layer of the substrate with a height of 35-40 mm under conditions of an aerated chamber with aeration on average during the lag phase, the exponential phase and in the slow growth stage of 1.5, 30 and 10 h kg lion, respectively, or in a thick layer substrate height 200 - 220 mm with volumetric aeration of the medium, which in average exponential medium is 1.0-1.2 DIA. The maximum enrichment of the substrate with protein is observed after 24 hours. The finished culture is dried at 105 ° C. Before drying due to fine-grained

the structure does not require the crushing of culture. The content of crude protein in the final product is 30-32%, including the content of the true protein is 24-26% E, recalculated to the DIA when the protein content in the raw material is 11-12%.,

Example 2. Process. Is carried out analogously to example 1 with the only difference that the nutrient medium has the following composition, wt.%:

Wheat bran45

Grape marc 52 Urea. 3

The seed is grown on the medium of the following composition, in wt.%:

g

Wheat bran Grape marc malt sprout extract (1: 5) (NH4) HPO

2.5 2.5

Kjhpo

0

10.0 0.10 0.12

Water the rest.

The content of crude protein in the final culture after 22 hours of cultivation of the producer is 30–32%, including the true protein 24–26% with 5 protein contents in the feed 12–12.5%.

Example 3. The process is carried out analogously to example 1 with the only difference that the nutrient medium has the following composition, wt%:

Wheat Bran43,5

Grape Marc 53.5 Urea3

The seed is grown on 5 medium of the following composition in wt.%: Wheat bran 3.0

Grape marc 2.2 Malt sprouts extract (1: 5) 8.5

(NH).,, 15

K.NRO; 0.15

Water the rest.

The protein content in the final culture — 24 hours after the cultivation of the producer — leaves 30–32%, including 24–26% true protein,

Example 4. The process is carried out analogously to examples 1-3, but block squeezes are used as vegetable matter. Maximal enrichment of substrate with protein. Observed after 20 h of cultivation of the Producer. The content of crude protein) of the 5 final product is 28%. True protein is 22% with a protein content in the raw material of 10.2%.

Example 5. The process is conducted similarly; examples 1-3, but as a source of 20 hrdov of plant origin, tomato sauce is used. Culturing is carried out for 24 hours. The content of crude protein in the final product is 40%, the true protein is 34% 25 when the protein content in the initial raw material is 21%.

Example 6. The process is carried out similarly to Examples 1-3, but as waste of plant origin, 30 No tea waste is used, which forms a tin in the production of tea drinks, fermentation takes 24 hours. The content of raw protein in the final product is 36%, true C5 protein is 28% with a protein content in the raw material of 16.5%.

Example 7. The process is carried out similarly to Examples 1-3, but starch production waste, potato pulp, is used as vegetable waste 40. Fermentation is carried out for 24 hours. The content of crude protein in the final product is 28.5%, the true protein is 22.2% at 45 the protein content of the raw material is 9.5%.

Example 8. The process is carried out similarly to examples 1-3, but as a waste of plant origin Q use geranium - a waste of essential oil production. Fermentation is carried out for 24 hours. The content of crude protein in the final product is 29.8%, the true protein is 22.5% with a protein content in the raw material of 11.3%.

Example 9. The process is carried out similarly to examples 1-3, but in quality (ltod of vegetable origin.

use the grapevine pruning, which is pretreated as follows: 50 kg of dry ground grapevine pruning is soaked in 100-110 l of 0.5-0.6 N. sulfuric or phosphoric acid solution, the obtained semi-liquid mass with a CB content of 26-28% is treated thermally at 110-120 ° C for 40-60 minutes. Urea is added to the treated medium so that the nitrogen introduced by it is 1.8% of the final air-dry medium. Urea serves simultaneously as a neutralizing agent. Then, the resulting mass is mixed with 50 kg of dried wheat bran, mixed thoroughly and sterilized at 1 atm. The moisture content of the final nutrient medium is 54%, pH 4.8-5.5, the medium is cooled, it is saturated to 35 ° C, and it is sown. Cultivation of wednes is 24 hours, the content of crude protein in horse-and-butter product is 32%, the true protein is 26% with a protein content in the raw material of 12.5%.

Claims (2)

Invention Formula 1 o A method for producing a feed protein supplement, which involves the preparation of inoculum, nutrient medium containing wheat bran as a source of carbon nutrition and additional - plant waste, wetting it, sterilization, sowing with Endo-mycopsis fibuligera strain, followed by solid phase fermentation and drying the finished product, characterized in that, in order to improve the quality of the product by enriching it with protein and other biologically:: active compounds and accelerating the and obtaining, as a strain of yeast when sowing, use Endomycopsis fibuligera C-4, and as a source of vegetable waste use grape or block, or tomato squeeze, or waste of tea production or essential oil production, or potato mash, or grapevine , at the same time, urea is additionally introduced into the nutrient medium and is used in the following ratio of ingredients included in it, wt%: Wheat bran 42-45 Plant raw materials 52-55 at .1507 Urea3.0-3.5 and the humidity of the medium is set at 52–54% with a pH of 4.8–5.5 by adding phosphoric acid, while the solid phase fermentation process is carried out to the maximum accumulation of protein in the target product. 2. Method POP.1, which differs from the fact that during the preparation of a nutrient medium containing grapevine pruning, mixing the ingredients, moistening and adjusting the pH is carried out simultaneously, at the same time preparing 26-28% slurry / grapevine scraps of 0.5-0.6 n, sulfuric or phosphoric acid, heated to 110-120 ° C, kept for 40-60 minutes, and then urea and wheat bran are introduced. Table 1 ten continuation of the table.I 0.64 .0, 44 0.94 ova 0.74 0.47 0.58 wa 3.45 0.95 Oh 75  0.71 traces 0.67 1.86 1.24 1.15 3.24 1.53 1.0 3.40 1, 26 1.60 1.57 0.39 1.55