SU1486163A1 - Method of separating spermia by mobility - Google Patents

Description

The invention relates to animal husbandry, in particular to the artificial insemination of animals. The aim of the invention is the separation of sperm with manezhny and oscillatory movements. Sperm is injected into the porous septum 3. Through the nozzle 1, the reservoir 2 and the septum 3 create a fluid flow at a speed equal to the speed of sperm movement in a fixed medium. In this case, spermins with active translational motion due to reotaxis will move against the flow of fluid, exit into reservoir 3 and be removed through nozzle 4. Dead spermine and semen with manege and oscillatory movements will be removed from the reservoir by fluid flow through nozzle 5. 2 sec. item f-ly, 1 ill.

1486163

The invention relates to animal husbandry and artificial insemination of animals.

The aim of the invention is the separation of sperm with manezhny and oscillatory movement. five

The drawing shows a device for implementing the method, a General view.

Example. Sperm is injected into the porous material and is exposed to it with a fluid flow at a speed equal to the speed of sperm movement in a stationary medium. This speed will be different depending on the type of animal or bird. For example, the speed of movement of rooster sperm in a stationary medium is 1 mm / min. As a liquid, an artificial standard medium can be used to dilute sperm of the corresponding animal or bird species. The temperature of the liquid should be equal to the body temperature of the animal. For rooster sperm, the temperature should be 41–42 ° C. 20 Being in the pores, spermine with active translational motion under the action of fluid flow acquire a directed movement against the fluid flow, i.e. the phenomenon of reotaxis occurs. Moving against the flow of fluid, the sperm with active translational movement leaves the porous material. This leads to the fact that they begin to accumulate in the front of the tank, losing the ability to reotaxis, because there is no contact with the fixed βθ surface.

Spermine with a manege and oscillatory movement, as well as dead spermine are not able to move against the liquid current. Therefore, the liquid carries them out of the porous material and carries them out of the device. 35

Research data showed that in the semen before the separation there is spermine with active translational, vibrational and manege movement, as well as dead spermine. Microscopic examination of the fluid that has passed through the device shows that there are no spermins in it with active translational motion, but there are a lot of sperm cells with manege and oscillatory motion and dead forms.

Microscopic examination of the fraction collected 45 from the lower channel of the device showed that it contains spermine with only active translational motion.

Thus, these studies clearly show that the proposed method provides high-quality separation of sperm with manege and oscillatory movement.

- A device for separating sperm by mobility consists of a nozzle 1 connected to a pump designed to create a slow current of fluid, a reservoir 2 for accumulating sperm with active translational movement and a partition 3 of a porous material installed in the middle part. At the bottom of the tank there is a pipe 4 for removal of sperm with active translational motion. In the back of the tank there is a pipe 5 for drainage. Introduced into the septum 3 of a porous substance, the spermine at a speed of flow of fluid equal to the speed of sperm in a stationary medium, will come into contact with a fixed surface of the porous substance and show the ability to reotaxis. Spermine with active translational movement moving against the flow of fluid and go to the front of the tank 2, where they accumulate. There is no porous substance in the reservoir, spermine is not in contact with a fixed surface and the reotaxis is not manifested. At the bottom of the tank there is a pipe 4 for their removal.

Spermin with manege and oscillatory movement, as well as fixed forms can not interfere with the flow of fluid and are removed from the porous substance in the pipe 5 to drain the fluid.

Claims (1)

Claim A method for separating sperm by motility, including placing sperm in a vessel with a porous septum, creating a flow of carrier fluid through it and selecting sperm for insemination, characterized in that, in order to separate sperm with manegean and oscillatory movements, semen is injected directly into the porous septum, and the flow of the carrier fluid is given a speed equal to the speed of sperm motion in a stationary medium, and a temperature equal to the body temperature of the animal, and the selection of sperm for insemination is carried out before the porous reformation dkoy.