SU1704775A1 - Device for collecting the animal embryos - Google Patents

Abstract

This invention relates to animal breeding biotechnology. The aim of the invention is to reduce the labor intensity and loss of washed embryos. In the sterilized separating funnel 1, the rinsing fluid from the uterine horn under the separating net 4 flows through the delivery tube 6 under the separation net 4, is filtered and removed through the discharge nozzle 3 without embryos. The residual liquid with the embryos is drained into Petri dishes without additional settling. 1 il.

Description

The invention relates to biotechnology of animal reproduction, in particular to devices for washing and collecting embryos of female cattle.

The purpose of the invention is to reduce the complexity and loss of washed self-donor embryos.

The drawing shows a device for collecting animal embryos, General view, axonometry, 10

A device for collecting animal embryos includes a transparent dividing funnel 1 with a lower drain pipe 2, an upper outlet pipe 3 located above the dividing net 4, located inside the dividing funnel 1 above the outlet of the fluid supply tube 6. The funnel is covered with a lid 5 with an opening for passing the feed tube 6. 20

The device operates as follows.

In a pre-sterilized transparent separation funnel 1, through the inlet tube 6, the washing fluid from the 25 uterine horns enters under the separation net 4, is filtered and removed through the upper outlet 3 without embryos from the funnel 1. Under the separation net 4 in the lower part of the separation funnel 1 30 there are embryos with a small amount of flushing fluid. In the same way, embryos can be washed off from the second horn of the uterus of a female donor. Since the volume of washing liquid with embryos in our funnel 1 and after washing out from the second uterine horn remains 1–2 Petri dishes in volume, it can be drained into them to further evaluate the quality of the embryos without resorting to the traditional 30-minute 40-hour settling in thermostat. To ensure complete collection of washed embryos, funnel 1 is slightly rinsed with 5-10 ml of washing liquid.

The size of the holes of the separation grid 4 depends on the size of the washed animal embryos. It was found that in cattle donor females at the 5th moment of transplantation on 7-8 days after fertilization, the size of full embryos is in the range of 180-200 microns. pigs and small cattle - 150-170 microns. Therefore, a calibrated separation grid is used to wash cattle embryos with a mesh size of 120-150 μm. pigs and small cattle - 100-120 microns. those. smaller than the diameter of the smallest full-fledged embryo of the corresponding animal species. Larger cells of the separation grid 4 allow the embryos to exit the funnel 1 with the washing liquid, and smaller ones complicate the filtering process and the outflow of fluid from the funnel 1.

Thus, the use of a device for collecting animal embryos allows reducing the laboriousness of the process of collecting embryos of donor females by a factor of 2–3 and eliminating the loss of washed full-fledged embryos during mechanical suction of the supernatant and their long settling in the washing medium in a thermostat. In addition, there is no need to purchase and use special thermostats.

Claims (1)

Claim A device for collecting animal embryos. comprising a transparent dividing funnel, a supply tube and a cover, characterized in that. in order to reduce the complexity and losses of the washed embryos, the funnel is equipped with a separation grid located above the output end of the feeding tube, and a branch pipe located above the separation grid.