SU1445634A1 - Method of assessing shadow-endurance of plants - Google Patents

Abstract

The invention relates to agriculture and can be used in the agricultural part of agrophytocenosis, as well as in breeding work. The goal is to accelerate the assessment and reduce labor costs. To implement the method, a sample of the sheet (its part) is placed in liquid nitrogen, excited and recorded at 77 K fluorescence, and the ratio of the intensity of fluorescence at 728 kg, (the maximum fluorescence of chlorophyll pigments included in the chlorophyll-protein complex of the first photosystem) to a similar magnitude of anne at 682 nm (maximum of fluo; epacenter of the short oligonic form of chlorophyll pygmmets. Subtracted in chlorophyll-proteinKOMBYN komg leks second photos of the system) .. The larger the ratio specified, the more shade tolerant e. 1 tab. Sj

Description

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The invention relates to agriculture, mainly to methods for assessing the resistance of plants to adverse environmental factors, and can be used, for example, in the agricultural part of agrophytocenosis - park culture phytocenosis, as well as in breeding work.

The purpose of the invention is to accelerate the assessment of plant shade and labor costs.

The method is carried out as follows.

For the test, a part of the sheet is fixed in a holder, representing, for example, two square metal plates with a thickness of 1.5-2.0 mm and a length of 23 mm with a hole in the center with a diameter of 8-10 mm, which are fastened together. Sample immediately to liquid nitrogen. The excitation and recording of fluorescence at 77 K is carried out on a spectrofluorometer e with a low-temperature attachment. The intensity of the excited light is 0.3-0.4 mW / cm 2. Mercury la- is used as the source of the exciting light. Well, the light of which passes through (() Ilter. HirrencHBHOCTb luminous flux is controlled by neutral light filters. The ratio of the intensity at the maximum fluorescence of the chlorophyll pigments included in the H. yrofil – protein complex of the first photosystem () intensity at the maximum of the short-wave fluorescence chlorophyll included in the chlorophyll-protein com of 1x of the second photosystem (Ifcai). The magnitude of this relation characterizes the genetically tolerance of plants.The more this nonhuman, the more shade-tolerant the plant.

Example. Eight species of evergreen plants with previously known characteristics of their relation to light are used for the experiment. Shade-tolerant plants - laurus lucer; schschy, Donsk aucuba monochrome, 11LUNN Crimean. The plants grow well in the light and in the shade - it is evergreen, Japanese spruce. Photophilous plants - z.ml ml small-fruited, rosemary pharmacy yucca aloelitis.

The table shows the values of Irae / ligt for the plant species studied. The table shows that the ratio I / / lesi characterizes shade tolerance of the

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teny. The larger the magnitude of this ratio, the shade-tolerant plant species.

In evaluating the significance of the difference between the mean values of bzg / lstt. For shade-tolerant plants and plants that grow well in the light and in the shade, with the number of observations p 53, 1x1,0t 2,06 (tTftu, 1,86 with P 0.05). When assessing the reliability of the difference between the average values of these quantities for plants growing well in the light and in theory and light-loving (p 57) - 4.34 (eb. 3.29 with P 0.001). For shade-tolerant and light-loving plants (p 64). 34.16 (1 table 3.29 at P 0.00.1). This proved the reliability of differences in size at Р 0.05 between different species tolerance of plants.

The proposed method allows you to quickly and accurately diagnose the shade tolerance of plants. The measurement of fixed samples can be carried out at any time convenient for the investigator. With the help of this Sosoba it is possible to select a variety of plants for the purpose of internal planting of industrial premises, artificial ecological systems and plant communities (parks, squares, etc.), as well as to select plants for shade tolerance during selection work.

Claims (1)

Isopic and Geni Formula A method for assessing shade tolerance of plants, including measuring the biophysical indicator characterizing the photosynthetic apparatus of leaves and determining the shade tolerance of plants by this indicator, characterized in that, in order to speed up the assessment and reduce labor costs, before measuring the biophysical icon, the leaves are fixed in liquid nitrogen, excite fluorescence of chlorophyll pigments, register it and calculate the ratio of intensity at the maximum fluorescence of chlorophyll pigments included in Chlorophyll-protein complex of the first photosystem, to the intensity at the maximum of the fluorescence of the short-wave form of chlorophyll pigments included in the chlorophyll-protein complex of the second photosystem, and judging by the magnitude of this ratio, the plants are less tolerant, the higher the value of yKa3a {h whom otnosheni. T a b l and a Shade tolerant plants Dwarf Wolf 3,95 ± 0,13 Aucuba Donska monochrome 3.88 0.11 3.39 ± 0.10 Crimean plush Plants grow well on and in the shade Box evergreen 3,11 - 0,11 Euonymus ponsky1,94- 0,07 Light-loving plants Ground nickel small fruit 2.86 - 0.06 Rosemary pharmacy2.67 0.05 Yucca aloelitis 2.50 ± 0.07 3.74 ± 0.08 3.02 ± 0.07 2.66 - 0.05