SU1388806A1 - Method of determining progress of pathological process in hepatic disease - Google Patents

Abstract

The invention relates to medicine, the detection of the activity of the pathological process in acute and prolonged viral chronic hepatitis and cirrhosis. The purpose of the invention is to simplify and reduce the morbidity of the method. For this, a blood sample (7.0-10.0 ml) from the ulnar vein is held at 18 hours for 1 h. Then, the supernatant is discharged into a flat-bottomed vessel and placed in a thermostat for 1 hour at 37 ° C. Liquid contents with lymphocytes are sucked into centrifuge tubes and centrifuged for 4 minutes at 1000 rpm. Lymphocytes implanted in this way are 0.01 MP injected intracutaneously in the region of the middle third of the forearm, and the stemspot activity of the pathological process is determined by the area of the hyperemia zone. with S (1

Description

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The invention relates to medicine, in particular to detecting the activity of the pathological process in acute and prolonged viral chronic hepatitis and cirrhosis.

The aim of the invention is to simplify and reduce the morbidity of the method. The method is carried out as follows.

: - From the ulnar vein of the patient, 7.0-10.0 ml of blood is taken and left for one hour at room temperature. The supernatant is poured into a vessel: with a flat bottom and placed in a thermo-: stat at +37 seconds for 1 hour. Liquid contents with lymphocytes are aspirated into centrifuge tubes and centrifuged for 4 minutes in a centrifuge at 1000 rpm. Lymphocytes isolated in this manner in a volume of 0, 1 ml are injected intracutaneously in the region of the middle third of the forearm.

Example 1. Patient A.I., 48 years old, was admitted to the gastroenterology department with a diagnosis of lyupoidny hepatitis. State of admission heavy. Pain in the right hypochondrium, joints of the hands, dyspeptic disorders. In childhood suffered acute viral hepatitis. The exacerbation of the disease marks in connection with the onset of the menopausal period. The patient was conducted in the department: NII appropriate examination and treatment. Along with the usual methods, the patient was subjected to an intracutaneous test with autolymphocytes. Upon receipt of these samples amounted to 35 mm (++++). Extreme degree of activity. After 10 days of treatment, the test was repeated, the data were within the same range. The patient was connected with hormone therapy and every 10 days thereafter the repetition of the test. These are 25 mm (+ -f +) and 17 mm (+). The condition improved significantly. In parallel, biochemical tests were repeated. Biochemical tests normalized more quickly. Than vnutrikozhna test.

And p and m e r 2. Patient VI, 21 years. He entered the gastroenterological department with a diagnosis of post-necrotic cirrhosis of the liver, portal hypertension, esophageal varices, hypersplenism. A patient suffered from childhood viral hepatitis. At the age of 18, he was called to the Council.

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After the physical overload, nasal and then gastric bleeding began, he was hospitalized, after a year an invalid of group II. On admission, the condition is moderately severe. The patient was examined and treated. In parallel with the biochemical test, an intracutaneous test with autolymphocytes was carried out. On admission, these samples were 40 mm (++++). Extreme degree of activity. After treatment, the condition improved significantly. Significantly improved biochemical tests, but these samples remained within 17 mm (++), i.e. autoimmune reactions in the liver did not stop.

Example 3. Patient I., 48 years. Entered the ward after acute viral hepatitis two years ago. Sometimes, after physical exertion, notes the severity and pain in the right hypochondrium. For two years, notes one aggravation. In addition to biochemical tests, he was subjected to an intracutaneous test with autolymphocytes. These samples for admission were 25 mm (+++), with a degree of activity. After the treatment, the biochemical tests returned to normal, and the hypersensitivity test was 15 (+), which underlines the chronicity of the process.

In alcoholic hepatitis, these samples are minor. After the cessation of alcohol, the sample is quickly normalized.

Example 4. Patient AA, 32 years. Found in the gastroenterological department about the exacerbation of chronic persistent hepatitis. In addition to the usual biochemical tests, the patient was subjected to an intracutaneous test with autolymphocytes. Sample data for receipt of 20 mm (+++). The average degree of activity. Against the background of treatment, the intracutaneous test and biochemical tests were normalized.

Example 5. T he flow form. Sick TA, 59 years old. Was in the hospital with a diagnosis of acute infectious hepatitis, icteric form, severe degree of severity. The hospital stay is 68 bed-days. In the first decade from the onset of the disease, bilirubin is 14.4 mg%, AsAt is 16 µM, the sublimate test is 1.5 mp, thymol is 16.4 units, the prothrombin index is 45%, the intradermal test with autolymphocytes is 30 mm (++++) ( extremely heavy degree of activity). At discharge: bilirubin 1.34 mg%, AsAt - 0.18 μM, sublimate sample 1.7 ml. Intracutaneous test with autolymphocyte 15 mm (+) on the background of basic therapy.

Example 6. Patient A.N., 21 years. He was in the hospital with a diagnosis of acute viral hepatitis, icteric form, moderate degree of severity. The duration of hospital stay is 54 hospital days. In the first decade from the onset of the disease, bilirubin was 4.16 mg%, the sublimate test was 1.36 ml, the thymol test was 14.1 units, AlAt was 4.68 μM, the prothrombin index was 88%, and the intradermal test was 19 mm with autolymphocytes ( ++) (average degree of activity). At discharge, bilirubin 0.86 mg% AlAt - 0.8 μM, sublimate test 1.68 ml, intracutaneous test with autolymphocytes 8 mm (+).

Example 7. Patient BV, 15 years. He was hospitalized for 21 bed-days with a diagnosis of acute infectious hepatitis, icteric form, mild illness. In the first decade from the onset of the disease, bilirubin 1.70 mg%, AlAt - 4.36 µM, mercuric chloride test 1.86 ml, thymol - 10.4 units, prothrombin index 84%. Intracutaneous test with autolymphocyte 9 mm (-). At discharge: bilirubin 0.76 mg% AlAt - 0.96 μM, sublimate sample 1.8 MP. Intradermal test with 6 mm (+) autolymphocytes (mild activity).

Thus, the proposed method allows, in comparison with the prototype, to reduce patient trauma and simplify the method of determining the degree of activity of the pathological process in liver diseases.

Claims (1)

Invention Formula The method of determining the degree of activity of the pathological process in liver diseases by examining the patient's body, characterized in that, in order to simplify and reduce the trauma of the method, the skin reactions of the body are investigated by intradermal administration of autolymphocytes to the middle third of the patient’s forearm in a volume of 0.1 MP and the presence of a zone of hyperemia ranging in size from 7 to 15 mm is determined by a slight degree of activity, from 16 to 20 mm — an average degree of activity, from 21 to 26 mm — a heavy degree of activity, over 26 mm — extremely severe with Epen activity of the pathological process in diseases of the liver.