SU1348368A1 - Device for sampling cultural liquid and checking it to units for growing aerobic microorganisms - Google Patents

Abstract

The invention relates to the microbiological, medical and food industries and is intended for the collection of microbiological samples and their control in plants for growing aerobic microorganisms. The purpose of the invention is to improve the quality (Lc: | CO 00 oo oo

Description

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sterilization of an external measuring chamber and circulating duct; a device and a type of remote chamber 1, plug-in; The circulating pipelines 6 of 6 to the fermenter 2 and the circulation booster are supplied with pipelines 15, 16, 20 "dp of antiseptic, water and sterile air supply and two shut-off valves 3 and 8 cut off from the circulation areas. the cavities of the locking 5 and 4 valves face the chamber and form a single channel sterilized by an antiseptic.

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The invention relates to MKJpo-biological, medical and food industries and is intended for aseptic sampling of microbiological samples, for example, culture fluid and its control in the process of aerosolism and organisms.

The aim of the invention Hi :: JT: ir; TC is the improvement of the quality of sterilization of a measuring gauge 1L k; lmery and 1: vortex.

The drawing shows a diagram of a pre, lagged arrangement

The device consists of a flap) | measuring 1, amers 1, with: with a sensor, sensors for automatic counterparts}, for example, pH pCO, pO ", eI, co-Hofi with fermenter 2 but enter / -nni into the chamber through check valves 3 and 4 and into the liquid through the zagorn / 11th valve 5, the cir- syl pyo1 H01 of the pipeline 6, which includes sp. 7 and shut-off valve 8, for1: x K.uananoi 9 and 10 and 11 and 12, connected to the valve bodies of 3 and 8 and 4 and 5, and of the cases, respectively, under the steam and condensate drainage, loadable valves 13 and 14, installed on the pipelines 15 and 16 of the supply of antiseptic and water hygiene, valve and valve 17 and pipe 18, with /; i; 5 with valve, valve cavity 5, shut-off valve-: 19) 1p water antiseptic, soy / Dyne other with subclavian valve cavity 4. tru8368

and the supraclavian cavities sterilized by steam are facing the circulation areas. In addition, the inlet and outlet antiseptic valves are connected to the subvalvular cavities, and the condensate outlet valves are connected to the supravalvular cavities and are equipped with shut-off devices, which are closed valve cavities and the condensate drain pipe 24. All shut-off valves in the device are designed as bellows valves. 1 hp F.-ly, 1 ill.

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air duct 20 for air poles with 3anopHfjiM valve 21 for supplying sterile air to er1H1ft 7. And the valve cavities of shut-off valves 3 and 4, located at the remote gauge chamber 1, form a single channel from state days and connected to pipelines 18 and 22. The supply of an oTBo ,, j antiseptic, and the over-valve cavities of the shut-off valves 8 and 3, located 1H) 1x on the circulation pipe, Gchepoovod 6, are connected to the tubing 23TI 24 for supplying steam and draining condensate. All shut-off valves are made in the form of bellows valves.

Ustrsch 1stst; o works as follows:).

At the end of the process (}) of completion and d) clearing the sampling system (11 purchasers. Liquid fluid, intake chamber 1), the chamber 1 is washed with water nil, for 0 0 for all closed valves л lsr,; valves 14, 17, 19 and 25. Water passes on tg: dummification} of the code 8, Bi.iHocHOi i measure: 1 -H1; th chamber 1 and C1 is passed through valves 19, section 25 in the bottom pipe; 26. 26. After a time ( But the valves 19, 17 and 14 will stop, at 1 TH, the camera 1 will remain alarm-free. Sterilization of the sample system can be carried out in conjunction with sterilization When the 1st sterilizer P1 is combined, it is opened up to 8-1, 12 and 27 for sterilizing forces, the background of valve 5, the circulation of the south pipeline 6, the airlift 7 and valve 8; valve 3 - to sterilize its cavity and bellows-side of valve 4. Steam enters the pipeline 23 as a result of opening valves 10, 28 and 9, the condensate is discharged into pipe 24 to drain condensate by opening valves 12, 11 and 27. After the specified sterilization time close the valves 8, 10 28 and 3 and open the valve 21 in line 20 for supplying with compressed air, while the area before the measuring chamber bounded by the bellows parts of valves 3 and 4 remains under vapor pressure, and the area after the camera bounded by the pressure parts of valves 5 and 8 is under air pressure.

The external measuring chamber 1 is sterilized by an antiseptic (for example, formalin spray), for which valves 13, 17 and 19, 25 are opened at the entrance and exit of the antiseptic from the chamber, while the chamber is freed from water and filled with an anesthetic. After a predetermined sterilization time has elapsed, the valves 13, 17 and 12 close and open the valve 5 to supply sterile compressed air to the chamber, forcing the antiseptic into the conduit 26 to drain to the sewage system. At the end of the purge, the system is considered as ready for operation.

To connect the system to the fermenter 2, close valves 9, 11, 19, and 25, open valves 3, 4, and 8. During operation of the device, in order to prevent infection from valves 17, 12, 19, and 11, and create thermoins, their bellows parts serve steam valve opening

29.30, 31 and 32, and the condensate is discharged into the pipeline 24 to drain the condensate with the valves 33 and 34 open.

During the fermentation, if necessary, the sampling system can be disconnected from the fermenter, for example, to replace electrodes, for which valves 8, 5, 4, 3, 21, 29 are closed,

30,31 and 32 and open the valves 10, 28 and 9 and the steam supply pipelines

to the bellows parts of valves 8, 21 and 3, 11 and 12 at the condensate outlet from

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bellows cavities of valves 4 and 5. After replacing the sensors, valves 10 and 28 on the steam supply pipe to the circulation pipe 6 and airlift 7 are closed and the valve 21 on the sterile compressed air supply on the pipe 20 is opened, while the cell area is under compressed pressure air, and an external measuring chamber is re-sterilized with an antiseptic as described.

During separate sterilization of the sampling system, valves 8 and 3 are in a closed state, and the remaining operations are carried out in the same sequence as during joint sterilization.

Claims (2)

1. A device for taking samples of the culture liquid and controlling it to plants dp of growing aerobic microorganisms, containing an external measuring chamber connected via a circulation pipe to the fermenter, stop valves installed on the circulation pipe, and pipelines with stop valves for steam supply, antiseptic water and sterile air, characterized in that, in order to increase the quality of the Sterilization of the remote measuring chamber and the circulation pipeline, it is equipped ruboprovodami for discharging condensate and antiseptic with isolation valves, the check valves subvalvular cavity located at remote measuring chamber, with the latter form a single channel and connected to a conduit for supplying and discharging antiseptic and supravalvular cavity shutoff valves located on. circulation pipe connected to the pipe for steam supply and removal of condensate. 2. The device according to claim 1, about tl and h and th y e. E with the fact that the shut-off valves are made in the form of bellows valves. D five 0 five 0 five