SU1332231A1 - Method of determining the content of dense substances in pespiration - Google Patents

Abstract

This invention relates to medicine. The purpose of the invention is to increase the reproducibility of the method. Nickel oxide powder is applied to a sheet of filter paper. The squares of this paper are placed in the comparator socket for immunological studies. A drop of sweat is applied to a sheet of indicator paper, incubated, and water is added. Filter the resulting solution into the neighboring slot of the comparator and add dimethylglyoxime. Accounting for the intensity of the reaction produced by caras. with colorimetric calibration scale. 3 tab. with o D D 00

Description

The invention relates to medicine, in particular to methods for determining the concentration of dense substances in sweat,

The purpose of the invention is to increase the reproducibility of the method.

The goal is achieved by introducing nickel oxide hydrate into the sweat fluid, and

the dissolved state after the chemical process reveals an order of magnitude more

The reaction with the sweat components of the metal ions is revealed by the introduction of dimethylglyoxin prior to the staining reaction, the intensity of which is proportional to the concentration of dissolved metal ions.

Example: A finely dispersed powder of nickel oxide hydrate with a module of 5.3 + 1.4 mg / cm is put on a sheet of filter paper and with a white stripe, and squares 1x1 cm in size are prepared from it. When carrying out the reaction, they are placed in the slot of a standard comparator .dl immunological studies. A drop of sweat (0.022 ml) collected with a micropipette directly from the surface of the test skin is applied to the comparator socket on a sheet of test paper, incubated for 15 minutes and then 1 ml of water is added. After an hour-long incubation, the resulting solution is filtered into the neighboring nest of the comparator and 0.5 ml of the developing reagent, dimethylglyoxime, is added to 0.3 ml of the filtrate according to the standard reaction of V.A. Chugaev. Accounting for the intensity of the reaction is carried out visually in comparison with a colorimetric calibration scale, which is prepared in the standard way 1 time in three months.

Table 1 shows the results of the study of the solubility of nickel hydroxide Ni (OH.) In mA / l in water, in a 10-component model of a sweat liquid of known composition and in the sweat of testers,

Table 2 presents data on the concentration of ions in various media, determined by the known (by the C1 ion in mA / l) and the proposed (by the ion in mA / l 10). in ways.

A physiological solution, containing only chlorine anions, was chosen as a reference for comparison with the indicators of sweat liquid of testers obtained in a known manner from the ionic groups of chlorine and the proposed method from the ionic groups of nickel.

As can be seen from Table 2, the amount of chlorine ions is determined significantly less than in physiological saline, in a known manner in the sweat of the testers.

five

high compared with saline values of dense substances in the sweat fluid.

The proposed method allows to capture not only chlorine ions, but also other anionic groups that are not trapped in a known manner.

Table 3 presents data on the concentration (in mA / l 10 Ni) of substances in the sweat of male and female testers, taken under production conditions, in athletes in training, and in untrained persons (sauna).

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Claims (1)

25 claims The method for determining the content of dense substances in a sweat by treating the sample with chemical reagents followed by determination by color reactions, in order to increase the reproducibility of the process, the treatment is carried out with nickel hydroxide powder, and the color reaction is carried out by adding dimethylglyoxime. Table 1 1332231 Table 2 Table3