SU1330560A1 - Method of determining corazol - Google Patents

Abstract

The invention relates to analytical chemistry, in particular the determination of corazol used in medicine. The goal is to simplify the way. The latter is carried out by dissolving the sample in an aqueous solution of sodium salicylate, followed by treatment of the solution with copper sulfate (2) and iodometric titration of the filtrate. The method provides high accuracy and reproducibility of the determination of corasol (quantitative deviations do not exceed t3%). 4 tab. (L oo oo ate O5

Description

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The invention relates to the analysis of chemical organic compounds, in particular, to methods for the quantitative determination of corasole (1,5-pentamethylene tetrazole).

The aim of the invention is to simplify the method.

The method is carried out as follows.

Example 1. Method for determining corazol in a substance.

About 0.25 g of corazol (exact mass) is placed in a dry conical flask with a capacity of 100 ml and dissolved in 5.00 ml of a 26% sodium salicylate solution. Then 40.00 ml of a 0.1 M solution of copper sulphate is added with stirring.

Within 1 minute, 5.00 ml of water is added and allowed to stir for 10 minutes while being periodically displaced. The liquid is filtered through a paper filter | (5-7 cm in diameter), covered with a watch glass during filtering of the funnel. The first 5-8 ml of filtrate is discarded, and 5 drops of diluted acetic acid, 1.5 g of potassium iodine and the separated iodine are titrated with 0.1 N sodium thiosulfate solution at the end of the titration slowly to 25 ml of clear filtrate (indicator-starch ) In parallel, a control experiment was carried out, for which 5 drops of diluted acetic acid, 1.5 g of potassium yoride and 1.5 g of iodine were added to 20.00 ml of 0.1N copper sulphate solution. a solution of thiosulfate (1 ml of O, 1N sodium thiosulfate solution corresponds to 0.01382 g of corazol).

The content of corasol in% (X) is calculated by the formula

X

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V V is the amount of ml of 0.1N sodium thiosulfate solution, followed by titration of 40 ml of 0.1N. copper sulfate solution; a quantity of 0.1 ml of sodium thiosulfate solution, followed by a titration of 25 ml of f ½ of altrate;

49.61 is the true volume resulting from the discharge of liquids due to the formation of a precipitate, ml; a - linkage,

The results of quantitative determination of corazol in the substance are listed in Table 1.

Comparative data for the determination of corazol by known and proposed methods are presented in Table 2.

PRI mme R 2. Method for determining corazol tablets 0.1 g

About 0.25 g (exact weight) of powdered tablets is placed in a dry conical flask per 100 ml. Then, the determination is continued as in the procedure for analyzing corazol in a substance, beginning with words and dissolved in 5.00 ml of a 26% solution of sodium salicylate and ending with 1 ml of 0.1 N sodium thiosulfate solution corresponds to O, 01382 g of corazol,

The content of corasol in 1 tablet in grams (X) is calculated

Y

(V - V,) -0.01382-b

where V is the number of ml of O, 1N sodium thiosulfate solution, followed by titration with 40 ml of copper sulfate solution;

V. Number of ml of 0.1N sodium thiosulfate solution, followed by titration with 25 ml of filtrate; a - weight, g; i — average weight of 1 tablet, g; .

49.61 is the true volume resulting from the discharge of liquids due to the formation of a precipitate, ml. The results are presented in table 3.

Method for the determination of corasol in 10% solution for injection (in ampoules).

2.5 ml of the test solution is placed in a dry 100 ml conical flask, 2.50 ml of water is added. Then continue the determination as specified in the method for analyzing corazol in

- one

substance, starting with words and diluted in 5.0 ml of 26% sodium salicylate solution.

The content of corasol in 1 ml of solution (X) is calculated by the formula

(V - v 2i | l) 0.01382

2.5

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V v. Number of ml of 0.1N sodium thiosulfate solution, followed by titration of 40 MP copper sulfate; Number of ml of 0.1N sodium thiosulfate solution, followed by titration with 25 ml of filtrate. The results are presented in section 4.

The proposed method should be used in chemical-pharmaceutical plants producing corazole, its dosage forms (tab 30560

tapes, ampoules), as well as in laboratories of pharmacy bases and control and analytical laboratories of pharmacy departments.

The proposed method, in comparison with the known method, provides the possibility of a simple and accessible determination of corasol with high reproducibility and accuracy.

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Claims (1)

Invention Formula The method of determining corazol by dissolving the analyzed sample, processing the resulting solution with copper salt, filtering the precipitated precipitate followed by idometric titration of the filtrate, so that, in order to simplify the process, the dissolution is carried out in an aqueous solution sodium salicylate and copper (II) sulfate is used as the copper salt. 25 Table 1 Editor A.Lezhnina Compiled by L.Rusanova Tehred M. Khodanich Proofreader No. King 3577/47 Circulation 776 Subscription VNIIPI USSR State Committee. For Inventions and Discoveries 113035, Moscow, Zh-35, Raushsk nab., 4/5 “Production and printing company, Uzhgorod, Projecto st., 4 Table 3 Table 4