SU1237978A1 - Method of determining delta to the power 5-androsten-3-beta-ol-17oh in urine - Google Patents

Abstract

. This invention relates to analytical chemistry and is intended for diagnosis in endocrinology. The purpose of the invention is to accelerate the process by carrying out the extraction with diethyl ether. A urine sample of IO ml with a pH of 7.5 is heated for 6 hours in a boiling water bath, cooled and transferred to a separatory funnel with the addition of double the volume of diethyl ether. The bottom layer of scum-. wow. 0.5 volume (by volume of the ether) sodium hydroxide solution is added to the ether extract, shaken. The bottom layer is discarded, the ether extract is washed with 0.5 volume of water. The extract is dried at 40–45 ° C for 12–18 min. A solution of furfural, glacial acid and sulfuric is added to the dry residue. They are placed in a water bath. After cooling, 3.0 ml of tetrachloroethane is poured, shaken, and the centrifuge is sprinkled. In the lower layer, the absorption coefficient is determined, which is directly proportional to the content of d-anatrosten-3 -ol-70H. From S W 1C 00 h from h 00

Description

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This invention relates to analytical chemistry, in particular to physicochemical analysis, and can be used in clinical medicine for the diagnosis of endocrinological diseases.

The aim of the invention is to accelerate the method

Example. A 10 ml urine sample with a pH of 7.5 is heated for 6 hours in a boiling water bath, cooled and transferred to a separatory funnel with the addition of double the volume of diethyl ether. The bottom layer is discarded, and 0.5 volumes of a volume of ether) of 10% sodium hydroxide solution is added to the ether extract, shaken for 1 minute. The bottom layer is discarded, the aspheric extract is drained with 0.5 volumes of water. The extract is dried at 45 ° C for 15 minutes. 0.8 ml of a 0.56% solution of furfural, 0.3 ml of glacial, 3.0 ml of 16 and sulfuric acid was added to the dry residue and placed for 12 min in a water bath at 70 ° C. After cooling the mixture 3.0 ml of tetrachloroethane, shake for 1-1.5 minutes. Centrifuged for 5 minutes at 1000-100 rpm. In the lower layer, the absorption coefficient is determined, which is directly proportional to the content

1237978I

Mr. androsten-3-ol-170N (DND). At the same time, blank samples and samples with crystalline DND (10, 20, 30, 40 µg) are placed.

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The absorption coefficient was measured at a wavelength of 688 nm. The lower limit of sensitivity is 5 µg, the sample volume is 3 ml, and the type 10 of the apparatus is a Hitachi recording spectrometer.

The range of linear dependence of the calibration curve is 10-40 μg. 15 The content of DNA in the urine was 60 µg.

Claims (1)

Formula Eteni 20 A method for determining U -androsten -3 / 3-ol-170N in urine by neutral hydrolysis of a test sample. Extraction and purification of the extract followed by spectrophotometry, characterized in that, in order to accelerate the process, extraction is carried out with diethyl ether followed by drying extract at 40-45 ° for 1230 18 min, and the selection of the colored complex is carried out by extraction with tetrachloroethane. 25 Editor N.Gorvat Compiled by N.Valeeva Tehred L.Serdyukova Order 3283/45 Circulation 778 Subscription VNIIPI USSR State Committee for inventions and discoveries P3035 / Moscow, Zh-35, Raushsk nab, 4/5 Production and printing company, Uzhgorod, st. Project, 4 Formula Eteni The method of determining U -androsten -3 / 3-ol-170N in urine by neutral hydrolysis of the sample under investigation. Extraction and purification of the extract followed by spectrophotometry, characterized in that, in order to accelerate the process, the extraction is carried out with diethyl ether followed by drying the extract at 40-45 0 for 1218 min, and the selection of the color complex is carried out by extraction with tetrachloroethane. Proofreader G. Reshetnik