SU1227198A1 - Method of producing polypeptides - Google Patents

Description

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This invention relates to the medical industry, in particular to the isolation of a biologically active substance from animal organs.

The purpose of the invention is to obtain 5 lipids that reduce cholesterol in the blood.

Example 1. 100 kg of large blood vessels of horses are taken. The vessels are cleaned, crushed. Grind- 10 vessels are loaded into the reactor, poured with 3% acetic acid solution (1 hour of raw materials and 6 parts of acetic acid solution) .. ZnClj is added to the solution at the rate of 1 g per 1 liter of 15 solution, extraction is carried out for 48 hours stirring 1 h. 3 times a day. The substrate is centrifuged, the supernatant is decanted, acetone is added at a temperature of 20 SRI 1: 6, mixed, the resulting precipitate is dried by filtration, crushed and dissolved in a 1% acetic acid solution at room temperature on the basis of 20 g of 25 powder in 1 l of a solution of acetic acid. After 2 h with continuous stirring, filter, remove the ballast substances. Acetic acid is added to the filtered solution to the lot to pH 4.5, sterile filtered through EKS plates, poured into vials to contain 10 mg of substance in each and lyophilized. The yield of the active substance is 1 kg. 35

P. Example 2. 5 kg of large blood vessels of sheep are taken as raw material. The vessels are cleaned, frozen and crushed. The prepared raw material is loaded into the reactor, pour 40 6 hours. 3% aqueous solution of acetic acid 12271982

With respect to 1 h, the raw material with added ZnCl, at the rate of 1 g per 1 l of the solution, extraction is carried out for 36 h, stirring 1 h 3 times a day. The substrate is centrifuged, the supernatant is decanted, acetone is added with a temperature of -4 ° C in a ratio of 1: 6, and the mixture is stirred. The precipitate obtained is dried and dissolved in a 1% solution of acetic acid at the rate of 20 g of powder per 1 l of solution of acetic acid. After 1 hour of continuous stirring, the ballast materials are removed by filtration.

The filtered solution is adjusted to pH 4.5, sterile filtered through EKS plates, poured into vials, set into each 10 mg of substance and lyophilized. Get 0.05 kg of the active substance.

Example 3. Take the vessels of pigs, sheep and calves in the amount of 200 kg, prepare them, grind and extracted with 3% solution of acetic acid with the addition of zinc chloride at the rate of 1 kg per 1 liter of acid for 72 hours, stirring. The substrate is centrifuged in a supernatant, then in a ratio of 1: 6, acetone is poured in and mixed.

The resulting precipitate is dried and dissolved in 1% acetic acid solution. After 3 hours with continuous stirring, the ballast 1e is filtered off.

The filtered solution is adjusted to pH 4.5 with acetic acid, filtered sterile, poured into vials containing 10 mg each, and lyophilized. The output of the active substance 2 kg.

Compiled by V. Brusyuszewska Editor N. Kishtulinets Tehred I. Popovich Corrector A.T.

Order 2235/4 Circulation 660 Subscription

VNIIPI USSR State Committee

for inventions and openings 113035, Moscow, Zh-35, Raushsk nab, d. 4/5

Production and printing company, Uzhgorod, st. Project, 4

Claims (1)

METHOD FOR PRODUCING POLYPEPTIDES, including extraction of crushed raw materials with a 3% aqueous solution of acetic acid in the presence of zinc chloride for 48-72 hours, centrifugation, treatment of the supernatant with acetone at a temperature of minus 3 to minus 5 ° C, followed by dissolution of the precipitate and filtering , characterized in that, in order to obtain polypeptides that lower cholesterol in the blood, the blood vessels of the animal are used as raw materials, and the sediment is dissolved in a 1% aqueous solution of acetic acid s. (/)>