RU1681427C - Method of preparing of agent enhancing antibacterial resistivity of lung - Google Patents
Method of preparing of agent enhancing antibacterial resistivity of lung Download PDFInfo
- Publication number
- RU1681427C RU1681427C SU4747745A RU1681427C RU 1681427 C RU1681427 C RU 1681427C SU 4747745 A SU4747745 A SU 4747745A RU 1681427 C RU1681427 C RU 1681427C
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- RU
- Russia
- Prior art keywords
- lung
- hours
- resistivity
- preparing
- target product
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
Description
Изобретение относится к медицинской промышленности и медицине и касается выделения биологически активных веществ из органического сырья. The invention relates to the medical industry and medicine and for the allocation of biologically active substances from organic raw materials.
Цель изобретения - повышение специфической активности целевого продукта. The purpose of the invention is to increase the specific activity of the target product.
П р и м е р. Берут 5 кг замороженной ткани легких крупного рогатого скота, измельчают на волчке. Сырье заливают 10-12 частями раствора уксусной кислоты до рН 3. Туда же добавляют 0,1 г на 1 л хлористого цинка и включают мешалку. Экстракцию проводят при 0оС в течение 24 ч при постоянном перемешивании.PRI me R. Take 5 kg of frozen tissue of the lungs of cattle, grind in a spinning top. The raw material is poured with 10-12 parts of a solution of acetic acid to pH 3. There, 0.1 g per 1 liter of zinc chloride is added and the mixer is turned on. The extraction is carried out at 0 about C for 24 hours with constant stirring.
По окончании экстракции субстрат сливают и центрифугируют 20 мин со скоростью 3000 об/мин. Надосадочную жидкость декантируют, к ней добавляют 12 ч. ацетона на 1 ч. субстрата при -3оС, перемешивают и формируют осадок 8 ч, после чего перемешивают и фильтруют.At the end of the extraction, the substrate is drained and centrifuged for 20 minutes at a speed of 3000 rpm. The supernatant was decanted and added thereto 12 hours. The acetone for 1 hour. The substrate at -3 ° C, and the formed precipitate was stirred for 8 hours, then stirred and filtered.
Полученный осадок высушивают, измельчают и растворяют в дистиллированной воде из расчета 100 г осадка на 5 л воды. В течение 2 ч раствор перемешивают и удаляют балластные белки фильтрованием. Фильтрат подвергают ультрафильтрации через полые волокна при комнатной температуре, давлении 0,6 кгс/см2 и скорости фильтрации 0,25 см/с. Ультрафильтрат лиофилизируют. Выход активного вещества 13 г из 1 кг сырья.The resulting precipitate is dried, crushed and dissolved in distilled water at the rate of 100 g of sediment in 5 l of water. The solution is stirred for 2 hours and the ballast proteins removed by filtration. The filtrate is subjected to ultrafiltration through hollow fibers at room temperature, a pressure of 0.6 kgf / cm 2 and a filtration rate of 0.25 cm / s. The ultrafiltrate is lyophilized. The output of the active substance 13 g from 1 kg of raw materials.
Вещество, получаемое по предлагаемому способу, приводит к достоверному повышению антибактериальной резистентности легких и ускорению ликвидации инфекционного процесса в органах дыхания в 2-3 раза по сравнению с прототипом, что подтверждается данными бактериологического и морфологического исследований легких. The substance obtained by the proposed method leads to a significant increase in the antibacterial resistance of the lungs and accelerates the elimination of the infectious process in the respiratory system by 2-3 times compared with the prototype, which is confirmed by the data of bacteriological and morphological studies of the lungs.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU4747745 RU1681427C (en) | 1989-09-11 | 1989-09-11 | Method of preparing of agent enhancing antibacterial resistivity of lung |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU4747745 RU1681427C (en) | 1989-09-11 | 1989-09-11 | Method of preparing of agent enhancing antibacterial resistivity of lung |
Publications (1)
Publication Number | Publication Date |
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RU1681427C true RU1681427C (en) | 1994-11-15 |
Family
ID=30441502
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU4747745 RU1681427C (en) | 1989-09-11 | 1989-09-11 | Method of preparing of agent enhancing antibacterial resistivity of lung |
Country Status (1)
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RU (1) | RU1681427C (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7625870B2 (en) | 2004-06-22 | 2009-12-01 | “Access Bioscience” CJSC | Peptide substance restoring respiratory organs function |
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1989
- 1989-09-11 RU SU4747745 patent/RU1681427C/en not_active IP Right Cessation
Non-Patent Citations (1)
Title |
---|
Муравьев. Технология лекарств. М.: Медицина, 1980, т.1, с.182, 183, 314-215. * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7625870B2 (en) | 2004-06-22 | 2009-12-01 | “Access Bioscience” CJSC | Peptide substance restoring respiratory organs function |
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Legal Events
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MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20070912 |