RU1681427C - Method of preparing of agent enhancing antibacterial resistivity of lung - Google Patents

Method of preparing of agent enhancing antibacterial resistivity of lung Download PDF

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Publication number
RU1681427C
RU1681427C SU4747745A RU1681427C RU 1681427 C RU1681427 C RU 1681427C SU 4747745 A SU4747745 A SU 4747745A RU 1681427 C RU1681427 C RU 1681427C
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RU
Russia
Prior art keywords
lung
hours
resistivity
preparing
target product
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Russian (ru)
Inventor
Г.М. Яковлев
В.Х. Хавинсон
А.Л. Кожемякин
О.В. Чайка
В.И. Семенова
Original Assignee
Яковлев Герман Михайлович
Хавинсон Владимир Хацкелевич
Кожемякин Андрей Леонидович
Чайка Олег Васильевич
Семенова Вера Ивановна
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Application filed by Яковлев Герман Михайлович, Хавинсон Владимир Хацкелевич, Кожемякин Андрей Леонидович, Чайка Олег Васильевич, Семенова Вера Ивановна filed Critical Яковлев Герман Михайлович
Priority to SU4747745 priority Critical patent/RU1681427C/en
Application granted granted Critical
Publication of RU1681427C publication Critical patent/RU1681427C/en

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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

FIELD: medicine, medicinal industry. SUBSTANCE: milled lung animal tissue is extracted with acetic acid at pH 2-4 for 24-48 h at 0 C with addition of zinc chloride (0.1-0.2 g/l) following by 10-15-fold excess of acetone at -3-10 C for 5-10 h. Prepared product is dissolved in distilled water, filtered, subjected for ultrafiltration and lyophilization. EFFECT: enhanced specific activity of the end product.

Description

Изобретение относится к медицинской промышленности и медицине и касается выделения биологически активных веществ из органического сырья. The invention relates to the medical industry and medicine and for the allocation of biologically active substances from organic raw materials.

Цель изобретения - повышение специфической активности целевого продукта. The purpose of the invention is to increase the specific activity of the target product.

П р и м е р. Берут 5 кг замороженной ткани легких крупного рогатого скота, измельчают на волчке. Сырье заливают 10-12 частями раствора уксусной кислоты до рН 3. Туда же добавляют 0,1 г на 1 л хлористого цинка и включают мешалку. Экстракцию проводят при 0оС в течение 24 ч при постоянном перемешивании.PRI me R. Take 5 kg of frozen tissue of the lungs of cattle, grind in a spinning top. The raw material is poured with 10-12 parts of a solution of acetic acid to pH 3. There, 0.1 g per 1 liter of zinc chloride is added and the mixer is turned on. The extraction is carried out at 0 about C for 24 hours with constant stirring.

По окончании экстракции субстрат сливают и центрифугируют 20 мин со скоростью 3000 об/мин. Надосадочную жидкость декантируют, к ней добавляют 12 ч. ацетона на 1 ч. субстрата при -3оС, перемешивают и формируют осадок 8 ч, после чего перемешивают и фильтруют.At the end of the extraction, the substrate is drained and centrifuged for 20 minutes at a speed of 3000 rpm. The supernatant was decanted and added thereto 12 hours. The acetone for 1 hour. The substrate at -3 ° C, and the formed precipitate was stirred for 8 hours, then stirred and filtered.

Полученный осадок высушивают, измельчают и растворяют в дистиллированной воде из расчета 100 г осадка на 5 л воды. В течение 2 ч раствор перемешивают и удаляют балластные белки фильтрованием. Фильтрат подвергают ультрафильтрации через полые волокна при комнатной температуре, давлении 0,6 кгс/см2 и скорости фильтрации 0,25 см/с. Ультрафильтрат лиофилизируют. Выход активного вещества 13 г из 1 кг сырья.The resulting precipitate is dried, crushed and dissolved in distilled water at the rate of 100 g of sediment in 5 l of water. The solution is stirred for 2 hours and the ballast proteins removed by filtration. The filtrate is subjected to ultrafiltration through hollow fibers at room temperature, a pressure of 0.6 kgf / cm 2 and a filtration rate of 0.25 cm / s. The ultrafiltrate is lyophilized. The output of the active substance 13 g from 1 kg of raw materials.

Вещество, получаемое по предлагаемому способу, приводит к достоверному повышению антибактериальной резистентности легких и ускорению ликвидации инфекционного процесса в органах дыхания в 2-3 раза по сравнению с прототипом, что подтверждается данными бактериологического и морфологического исследований легких. The substance obtained by the proposed method leads to a significant increase in the antibacterial resistance of the lungs and accelerates the elimination of the infectious process in the respiratory system by 2-3 times compared with the prototype, which is confirmed by the data of bacteriological and morphological studies of the lungs.

Claims (1)

СПОСОБ ПОЛУЧЕНИЯ ВЕЩЕСТВА, ПОВЫШАЮЩЕГО АНТИБАКТЕРИАЛЬНУЮ РЕЗИСТЕНТНОСТЬ ЛЕГКИХ, включающий измельчение органического сырья, экстракцию, фильтрование и сушку, отличающийся тем, что, с целью повышения специфической активности целевого продукта, измельченную ткань экстрагируют уксусной кислотой при рН 2 - 4 в течение 24 - 48 ч при 0oС с добавлением 0,1 - 0,2 г/л хлористого цинка, осаждение целевого продукта проводят ацетоном в соотношении 1 : 10 - 1 : 15 при температуре - 3 - 10oС в течение 5 - 10 ч, осадок растворяют в дистиллированной воде, фильтруют, фильтрат подвергают ультрафильтрации при комнатной температуре, а ультрафильтрат лиофилизируют.METHOD FOR PRODUCING A SUBSTANCE INCREASING ANTIBACTERIAL LIGHT RESISTANCE, including grinding of organic raw materials, extraction, filtering and drying, characterized in that, in order to increase the specific activity of the target product, the crushed tissue is extracted with acetic acid at pH 2-4 for 24 to 48 hours 0 o With the addition of 0.1 - 0.2 g / l of zinc chloride, the precipitation of the target product is carried out with acetone in a ratio of 1: 10 - 1: 15 at a temperature of - 3 - 10 o C for 5 to 10 hours, the precipitate is dissolved in distilled water, filtered, filter t is subjected to ultrafiltration at room temperature, and the ultrafiltrate is lyophilized.
SU4747745 1989-09-11 1989-09-11 Method of preparing of agent enhancing antibacterial resistivity of lung RU1681427C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU4747745 RU1681427C (en) 1989-09-11 1989-09-11 Method of preparing of agent enhancing antibacterial resistivity of lung

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Application Number Priority Date Filing Date Title
SU4747745 RU1681427C (en) 1989-09-11 1989-09-11 Method of preparing of agent enhancing antibacterial resistivity of lung

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RU1681427C true RU1681427C (en) 1994-11-15

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7625870B2 (en) 2004-06-22 2009-12-01 “Access Bioscience” CJSC Peptide substance restoring respiratory organs function

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Муравьев. Технология лекарств. М.: Медицина, 1980, т.1, с.182, 183, 314-215. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7625870B2 (en) 2004-06-22 2009-12-01 “Access Bioscience” CJSC Peptide substance restoring respiratory organs function

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Effective date: 20070912