SU122339A1 - Method for determining amount of protein in milk, etc. liquids and apparatus for this method - Google Patents
Method for determining amount of protein in milk, etc. liquids and apparatus for this methodInfo
- Publication number
- SU122339A1 SU122339A1 SU619970A SU619970A SU122339A1 SU 122339 A1 SU122339 A1 SU 122339A1 SU 619970 A SU619970 A SU 619970A SU 619970 A SU619970 A SU 619970A SU 122339 A1 SU122339 A1 SU 122339A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- protein
- milk
- liquids
- determining amount
- amount
- Prior art date
Links
Description
Количество белка в молоке и других биологических жидкост х обычио определ етс химическим сиособом иутем обработки жидкости .различными химическими веществами. Этот способ требует сравнительно длительиого врелгени, затрат определеиного количества химических веществ и неудобен дл эксирессиых исследоваиий.The amount of protein in milk and other biological fluids can be determined by chemical means and the way the liquid is treated with various chemicals. This method requires a relatively long time, the cost of a certain number of chemical substances and is inconvenient for excision studies.
Описываемый способ не требует х М1 ческой обработки жидкости и может дать быстрый результат. Ofi основан на том, что количество белка определ ют ио стеиеии интенсивности с|)луоресцеиции содержащн.хс в белке молекул аминокислот - тирозина и.триптофана. The described method does not require liquid treatment of the liquid and can give a quick result. Ofi is based on the fact that the amount of protein is determined by the intensity of intensity with the |) lorescence of the contained xc in the protein of amino acid molecules - tyrosine and tryptophan.
При этом предполагаетс посто нство количественного содержани этих аминокислот в основном белке молока и пр ма иропорциоиальиость интенсивности флуоресценции от концеитрации тирозн ;а и триптофана .In this case, the constancy of the quantitative content of these amino acids in the basic protein of milk and the direct proportionality of the fluorescence intensity from the termination of thyroid awareness, and tryptophan are assumed.
По этому способу, исследуемую .жидкость подвергают действию лучей , .вызывающйх флуоресцеицню аминокислот, и затем измер ют H Iтенсивиость флуоресценции.Прибор дл определени количества белка в молоке по интенсивности флуоресцеицин тнрозина и триитофана изображен на чертеже.In this method, the test liquid is exposed to rays causing fluorescent amino acids, and then the fluorescence intensity is measured. An instrument for determining the amount of protein in milk by the intensity of fluorescein tyrosine and triitofan is shown in the figure.
В приборе имеетс ртутио-кварцева лампа /, лучи котЬрой через кондепсорную линзу 2 и кварцево-хлорный фильтр 3 падают-на кювету 4. Последн составлена из двух ультрафиолетовых-фильтров различной плотноетн, между которыми помещаетс исследуема жидкость. Сзади кюветы 4 помещен фильтр 5 из уранилового стекла, служащий дл зсилени нотока флуоресцеицин, а за фильтром 5-фотоэлемент 6, включенный в общую электрическую сеть с мнкроамнерметром 7.The device has a mercury-quartz lamp /, the rays of which through a condensation lens 2 and a quartz-chlorine filter 3 fall — onto a cuvette 4. The latter is composed of two ultraviolet filters of different tightness between which the liquid under study is placed. Behind the cuvette 4 there is a filter 5 made of uranyl glass, which serves to strengthen the flux of the flux cell, and behind the filter there is a 5 photo cell 6 connected to the common electrical network with an optical meter 7.
Отфильтрованный свет от ртутно-кварцевой лампы надает на первый светофильтр (например, тинаУФС-1) кюветы, возбуждает флуоресFiltered light from a mercury-quartz lamp impinges on a first light filter (for example, a TinaUFS-1) cell, excites a fluorescent light.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU619970A SU122339A1 (en) | 1959-02-17 | 1959-02-17 | Method for determining amount of protein in milk, etc. liquids and apparatus for this method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU619970A SU122339A1 (en) | 1959-02-17 | 1959-02-17 | Method for determining amount of protein in milk, etc. liquids and apparatus for this method |
Publications (1)
Publication Number | Publication Date |
---|---|
SU122339A1 true SU122339A1 (en) | 1959-11-30 |
Family
ID=48394038
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU619970A SU122339A1 (en) | 1959-02-17 | 1959-02-17 | Method for determining amount of protein in milk, etc. liquids and apparatus for this method |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU122339A1 (en) |
-
1959
- 1959-02-17 SU SU619970A patent/SU122339A1/en active
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